5nkw - Revision history

OCA at 13:03, 15 November 2023

OCA — Wed, 15 Nov 2023 13:03:51 GMT

←Older revision		Revision as of 13:03, 15 November 2023
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	<StructureSection load='5nkw' size='340' side='right'caption='[[5nkw]], [[Resolution\|resolution]] 1.48Å' scene=''>		<StructureSection load='5nkw' size='340' side='right'caption='[[5nkw]], [[Resolution\|resolution]] 1.48Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5nkw]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Lentinus_similis Lentinus~~ similis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NKW OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5NKW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5nkw]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Panus_similis Panus similis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NKW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5NKW FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=~~NAG~~:N-~~ACETYL~~-~~D-GLUCOSAMINE~~'>~~NAG~~</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.48Å</td></tr>
-	~~<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat">~~<scene name='pdbligand=~~HIC~~:4-~~METHYL~~-~~HISTIDINE~~'>~~HIC~~</scene></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=HIC:4-METHYL-HISTIDINE'>HIC</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5nkw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nkw OCA], [~~http~~://pdbe.org/5nkw PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5nkw RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5nkw PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5nkw ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5nkw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nkw OCA], [https://pdbe.org/5nkw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5nkw RCSB], [https://www.ebi.ac.uk/pdbsum/5nkw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5nkw ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A0S2GKZ1_9APHY A0A0S2GKZ1_9APHY]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 24:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Lentinus~~ similis]]	+	[[Category: Panus similis]]
-	[[Category: Frandsen~~, K E.H~~]]	+	[[Category: Frandsen KEH]]
-	[[Category: Leggio, L Lo]]	+	[[Category: Lo Leggio L]]
-	[[Category: Poulsen, J ~~C.N~~]]	+	[[Category: Poulsen J-CN]]
-	[[Category: Tandrup, T]]	+	[[Category: Tandrup T]]
-	~~[[Category: Enzyme]]~~	+
-	~~[[Category: Unknown function~~]]	+

OCA at 12:07, 26 August 2020

OCA — Wed, 26 Aug 2020 12:07:02 GMT

←Older revision		Revision as of 12:07, 26 August 2020
Line 1:		Line 1:

	==X-ray crystal structure of an AA9 LPMO==		==X-ray crystal structure of an AA9 LPMO==
-	<StructureSection load='5nkw' size='340' side='right' caption='[[5nkw]], [[Resolution\|resolution]] 1.48Å' scene=''>	+	<StructureSection load='5nkw' size='340' side='right'caption='[[5nkw]], [[Resolution\|resolution]] 1.48Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5nkw]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NKW OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5NKW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5nkw]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Lentinus_similis Lentinus similis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NKW OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5NKW FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=HIC:4-METHYL-HISTIDINE'>HIC</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=HIC:4-METHYL-HISTIDINE'>HIC</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5nkw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nkw OCA], [http://pdbe.org/5nkw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5nkw RCSB], [http://www.ebi.ac.uk/pdbsum/5nkw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5nkw ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5nkw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nkw OCA], [http://pdbe.org/5nkw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5nkw RCSB], [http://www.ebi.ac.uk/pdbsum/5nkw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5nkw ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 21:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Lentinus similis]]
	[[Category: Frandsen, K E.H]]		[[Category: Frandsen, K E.H]]
	[[Category: Leggio, L Lo]]		[[Category: Leggio, L Lo]]

OCA at 06:09, 1 November 2017

OCA — Wed, 01 Nov 2017 06:09:21 GMT

←Older revision		Revision as of 06:09, 1 November 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5nkw is ~~ON HOLD until Paper~~ Publication	+	==X-ray crystal structure of an AA9 LPMO==
		+	<StructureSection load='5nkw' size='340' side='right' caption='[[5nkw]], [[Resolution\|resolution]] 1.48Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5nkw]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NKW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5NKW FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=HIC:4-METHYL-HISTIDINE'>HIC</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5nkw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nkw OCA], [http://pdbe.org/5nkw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5nkw RCSB], [http://www.ebi.ac.uk/pdbsum/5nkw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5nkw ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Lytic polysaccharide monooxygenases (LPMOs) are industrially important copper-dependent enzymes that oxidatively cleave polysaccharides. Here we present a functional and structural characterization of two closely related AA9-family LPMOs from Lentinus similis (LsAA9A) and Collariella virescens (CvAA9A). LsAA9A and CvAA9A cleave a range of polysaccharides, including cellulose, xyloglucan, mixed-linkage glucan and glucomannan. LsAA9A additionally cleaves isolated xylan substrates. The structures of CvAA9A and of LsAA9A bound to cellulosic and non-cellulosic oligosaccharides provide insight into the molecular determinants of their specificity. Spectroscopic measurements reveal differences in copper co-ordination upon the binding of xylan and glucans. LsAA9A activity is less sensitive to the reducing agent potential when cleaving xylan, suggesting that distinct catalytic mechanisms exist for xylan and glucan cleavage. Overall, these data show that AA9 LPMOs can display different apparent substrate specificities dependent upon both productive protein-carbohydrate interactions across a binding surface and also electronic considerations at the copper active site.

-	~~Authors: Tandrup, T~~., Frandsen, ~~K.E.H.~~, ~~Poulsen~~, ~~J.-C.~~N., Lo Leggio, L.	+	Structural and electronic determinants of lytic polysaccharide monooxygenase reactivity on polysaccharide substrates.,Simmons TJ, Frandsen KEH, Ciano L, Tryfona T, Lenfant N, Poulsen JC, Wilson LFL, Tandrup T, Tovborg M, Schnorr K, Johansen KS, Henrissat B, Walton PH, Lo Leggio L, Dupree P Nat Commun. 2017 Oct 20;8(1):1064. doi: 10.1038/s41467-017-01247-3. PMID:29057953<ref>PMID:29057953</ref>

-	~~Description~~: ~~Auxiliary activity 9~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
-	[[Category: ~~Poulsen~~, ~~J.-C.N~~]]	+	<div class="pdbe-citations 5nkw" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Frandsen~~, K.~~E.H~~]]	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Frandsen, K E.H]]
		+	[[Category: Leggio, L Lo]]
		+	[[Category: Poulsen, J C.N]]
	[[Category: Tandrup, T]]		[[Category: Tandrup, T]]
-	[[Category: ~~Lo Leggio, L~~]]	+	[[Category: Enzyme]]
		+	[[Category: Unknown function]]

OCA at 09:25, 4 May 2017

OCA — Thu, 04 May 2017 09:25:51 GMT

←Older revision		Revision as of 09:25, 4 May 2017
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5nkw is ON HOLD	+	The entry 5nkw is ON HOLD until Paper Publication

	Authors: Tandrup, T., Frandsen, K.E.H., Poulsen, J.-C.N., Lo Leggio, L.		Authors: Tandrup, T., Frandsen, K.E.H., Poulsen, J.-C.N., Lo Leggio, L.

OCA: Protected "5nkw" [edit=sysop:move=sysop]

OCA — Wed, 12 Apr 2017 08:06:56 GMT

Protected "5nkw" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:06, 12 April 2017

OCA: New page: '''Unreleased structure''' The entry 5nkw is ON HOLD Authors: Tandrup, T., Frandsen, K.E.H., Poulsen, J.-C.N., Lo Leggio, L. Description: Auxiliary activity 9 [[Category: Unreleased St...

OCA — Wed, 12 Apr 2017 08:06:55 GMT

New page: '''Unreleased structure''' The entry 5nkw is ON HOLD Authors: Tandrup, T., Frandsen, K.E.H., Poulsen, J.-C.N., Lo Leggio, L. Description: Auxiliary activity 9 [[Category: Unreleased St...

New page

'''Unreleased structure'''

The entry 5nkw is ON HOLD

Authors: Tandrup, T., Frandsen, K.E.H., Poulsen, J.-C.N., Lo Leggio, L.

Description: Auxiliary activity 9
[[Category: Unreleased Structures]]
[[Category: Poulsen, J.-C.N]]
[[Category: Frandsen, K.E.H]]
[[Category: Tandrup, T]]
[[Category: Lo Leggio, L]]