5nkx - Revision history

OCA at 13:03, 15 November 2023

OCA — Wed, 15 Nov 2023 13:03:56 GMT

←Older revision		Revision as of 13:03, 15 November 2023
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	==HRSV M2-1 core domain, P3221 crystal form==		==HRSV M2-1 core domain, P3221 crystal form==
-	<StructureSection load='5nkx' size='340' side='right' caption='[[5nkx]], [[Resolution\|resolution]] 2.00Å' scene=''>	+	<StructureSection load='5nkx' size='340' side='right'caption='[[5nkx]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5nkx]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Hrsv Hrsv~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NKX OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5NKX FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5nkx]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Human_orthopneumovirus Human orthopneumovirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NKX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5NKX FirstGlance]. <br>
-	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">M2-1, ~~M2 (~~[~~http://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=11250 HRSV])~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.0000868Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5nkx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nkx OCA], [~~http~~://pdbe.org/5nkx PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5nkx RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5nkx PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5nkx ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5nkx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nkx OCA], [https://pdbe.org/5nkx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5nkx RCSB], [https://www.ebi.ac.uk/pdbsum/5nkx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5nkx ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q4KRW3_HRSV Q4KRW3_HRSV]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 16:		Line 18:
	</div>		</div>
	<div class="pdbe-citations 5nkx" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5nkx" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Elongation factor 3D structures\|Elongation factor 3D structures]]
		+	*[[M2 protein\|M2 protein]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Hrsv~~]]	+	[[Category: Human orthopneumovirus]]
-	[[Category: ~~Hernandez, Y Almeida~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Josts, I~~]]	+	[[Category: Almeida Hernandez Y]]
-	[[Category: ~~Molina,~~ I G]]	+	[[Category: Josts I]]
-	[[Category: ~~Pray-Gay, G de~~]]	+	[[Category: Molina IG]]
-	[[Category: Tidow, H]]	+	[[Category: Tidow H]]
-	[[Category: ~~Viral protein~~]]	+	[[Category: De Pray-Gay G]]

OCA at 06:26, 18 April 2018

OCA — Wed, 18 Apr 2018 06:26:54 GMT

←Older revision		Revision as of 06:26, 18 April 2018
Line 3:		Line 3:
	<StructureSection load='5nkx' size='340' side='right' caption='[[5nkx]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='5nkx' size='340' side='right' caption='[[5nkx]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5nkx]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NKX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5NKX FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5nkx]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Hrsv Hrsv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NKX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5NKX FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5nkx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nkx OCA], [http://pdbe.org/5nkx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5nkx RCSB], [http://www.ebi.ac.uk/pdbsum/5nkx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5nkx ProSAT]</span></td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">M2-1, M2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11250 HRSV])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5nkx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nkx OCA], [http://pdbe.org/5nkx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5nkx RCSB], [http://www.ebi.ac.uk/pdbsum/5nkx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5nkx ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Human syncytial respiratory virus is a nonsegmented negative-strand RNA virus with serious implications for respiratory disease in infants, and has recently been reclassified into a new family, Pneumoviridae. One of the main reasons for this classification is the unique presence of a transcriptional antiterminator, called M2-1. The puzzling mechanism of action of M2-1, which is a rarity among antiterminators in viruses and is part of the RNA polymerase complex, relies on dissecting the structure and function of this multidomain tetramer. The RNA-binding activity is located in a monomeric globular `core' domain, a high-resolution crystal structure of which is now presented. The structure reveals a compact domain which is superimposable on the full-length M2-1 tetramer, with additional electron density for the C-terminal tail that was not observed in the previous models. Moreover, its folding stability was determined through chemical denaturation, which shows that the secondary and tertiary structure unfold concomitantly, which is indicative of a two-state equilibrium. These results constitute a further step in the understanding of this unique RNA-binding domain, for which there is no sequence or structural counterpart outside this virus family, in addition to its implications in transcription regulation and its likeliness as an antiviral target.
		+
		+	Structure and stability of the Human respiratory syncytial virus M2-1 RNA-binding core domain reveals a compact and cooperative folding unit.,Molina IG, Josts I, Almeida Hernandez Y, Esperante S, Salgueiro M, Garcia Alai MM, de Prat-Gay G, Tidow H Acta Crystallogr F Struct Biol Commun. 2018 Jan 1;74(Pt 1):23-30. doi:, 10.1107/S2053230X17017381. Epub 2017 Dec 15. PMID:29372904<ref>PMID:29372904</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5nkx" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Hrsv]]
	[[Category: Hernandez, Y Almeida]]		[[Category: Hernandez, Y Almeida]]
	[[Category: Josts, I]]		[[Category: Josts, I]]

OCA at 05:44, 3 January 2018

OCA — Wed, 03 Jan 2018 05:44:53 GMT

←Older revision		Revision as of 05:44, 3 January 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5nkx ~~is ON HOLD until Paper Publication~~	+	==HRSV M2-1 core domain, P3221 crystal form==
-		+	<StructureSection load='5nkx' size='340' side='right' caption='[[5nkx]], [[Resolution\|resolution]] 2.00Å' scene=''>
-	~~Authors~~: ~~Hernandez, Y~~.A.~~, Josts, I~~.~~, Molina, I~~.G.~~, de Pray~~-~~Gay, G~~.~~, Tidow, H~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5nkx]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NKX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5NKX FirstGlance]. <br>
-	~~Description~~: ~~HRSV M2~~-~~1 core domain~~, ~~P3221 crystal form~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5nkx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nkx OCA], [http://pdbe.org/5nkx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5nkx RCSB], [http://www.ebi.ac.uk/pdbsum/5nkx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5nkx ProSAT]</span></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[[~~Category~~: ~~De Pray~~-~~Gay, G]~~]	+	__TOC__
-	[[Category: ~~Molina~~, ~~I.G~~]]	+	</StructureSection>
		+	[[Category: Hernandez, Y Almeida]]
	[[Category: Josts, I]]		[[Category: Josts, I]]
-	[[Category: ~~Hernandez~~, ~~Y.A~~]]	+	[[Category: Molina, I G]]
		+	[[Category: Pray-Gay, G de]]
	[[Category: Tidow, H]]		[[Category: Tidow, H]]
		+	[[Category: Viral protein]]

OCA at 11:34, 27 September 2017

OCA — Wed, 27 Sep 2017 11:34:19 GMT

←Older revision		Revision as of 11:34, 27 September 2017
Line 3:		Line 3:
	The entry 5nkx is ON HOLD until Paper Publication		The entry 5nkx is ON HOLD until Paper Publication

-	Authors: Hernandez, Y.A., Tidow, H.	+	Authors: Hernandez, Y.A., Josts, I., Molina, I.G., de Pray-Gay, G., Tidow, H.

	Description: HRSV M2-1 core domain, P3221 crystal form		Description: HRSV M2-1 core domain, P3221 crystal form
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
		+	[[Category: De Pray-Gay, G]]
		+	[[Category: Molina, I.G]]
		+	[[Category: Josts, I]]
	[[Category: Hernandez, Y.A]]		[[Category: Hernandez, Y.A]]
	[[Category: Tidow, H]]		[[Category: Tidow, H]]

OCA: Protected "5nkx" [edit=sysop:move=sysop]

OCA — Wed, 12 Apr 2017 08:07:08 GMT

Protected "5nkx" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:07, 12 April 2017

OCA: New page: '''Unreleased structure''' The entry 5nkx is ON HOLD until Paper Publication Authors: Hernandez, Y.A., Tidow, H. Description: HRSV M2-1 core domain, P3221 crystal form [[Category: Unre...

OCA — Wed, 12 Apr 2017 08:07:07 GMT

New page: '''Unreleased structure''' The entry 5nkx is ON HOLD until Paper Publication Authors: Hernandez, Y.A., Tidow, H. Description: HRSV M2-1 core domain, P3221 crystal form [[Category: Unre...

New page

'''Unreleased structure'''

The entry 5nkx is ON HOLD until Paper Publication

Authors: Hernandez, Y.A., Tidow, H.

Description: HRSV M2-1 core domain, P3221 crystal form
[[Category: Unreleased Structures]]
[[Category: Hernandez, Y.A]]
[[Category: Tidow, H]]