5nmw - Revision history

OCA at 13:09, 15 November 2023

OCA — Wed, 15 Nov 2023 13:09:34 GMT

←Older revision		Revision as of 13:09, 15 November 2023
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	<StructureSection load='5nmw' size='340' side='right'caption='[[5nmw]], [[Resolution\|resolution]] 1.89Å' scene=''>		<StructureSection load='5nmw' size='340' side='right'caption='[[5nmw]], [[Resolution\|resolution]] 1.89Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5nmw]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NMW OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5NMW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5nmw]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Zonocerus_variegatus Zonocerus variegatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NMW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5NMW FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.89Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5nmw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nmw OCA], [~~http~~://pdbe.org/5nmw PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5nmw RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5nmw PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5nmw ProSAT]</span></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5nmw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nmw OCA], [https://pdbe.org/5nmw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5nmw RCSB], [https://www.ebi.ac.uk/pdbsum/5nmw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5nmw ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/L0N8S9_9ORTH L0N8S9_9ORTH]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Faust, A]]	+	[[Category: Zonocerus variegatus]]
-	[[Category: Kubitza, C]]	+	[[Category: Faust A]]
-	[[Category: Ober, D]]	+	[[Category: Kubitza C]]
-	[[Category: Scheidig, A]]	+	[[Category: Ober D]]
-	~~[[Category: Fad]]~~	+	[[Category: Scheidig A]]
-	~~[[Category: Flavin-containing monooxygenase]]~~	+
-	~~[[Category: Oxidoreductase]]~~	+
-	~~[[Category: Pyrrolizidine alkaloid n-oxygenase]]~~	+
-	~~[[Category: Rossmann fold]]~~	+
-	~~[[Category: Senecionine]]~~	+
-	~~[[Category: Two dinucleotide binding domain flavoprotein~~]]	+

OCA at 12:08, 26 August 2020

OCA — Wed, 26 Aug 2020 12:08:19 GMT

←Older revision		Revision as of 12:08, 26 August 2020
Line 1:		Line 1:

	==Crystal Structure of the pyrrolizidine alkaloid N-oxygenase from Zonocerus variegatus in complex with FAD==		==Crystal Structure of the pyrrolizidine alkaloid N-oxygenase from Zonocerus variegatus in complex with FAD==
-	<StructureSection load='5nmw' size='340' side='right' caption='[[5nmw]], [[Resolution\|resolution]] 1.89Å' scene=''>	+	<StructureSection load='5nmw' size='340' side='right'caption='[[5nmw]], [[Resolution\|resolution]] 1.89Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5nmw]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NMW OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5NMW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5nmw]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NMW OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5NMW FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5nmw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nmw OCA], [http://pdbe.org/5nmw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5nmw RCSB], [http://www.ebi.ac.uk/pdbsum/5nmw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5nmw ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5nmw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nmw OCA], [http://pdbe.org/5nmw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5nmw RCSB], [http://www.ebi.ac.uk/pdbsum/5nmw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5nmw ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	</div>		</div>
	<div class="pdbe-citations 5nmw" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5nmw" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Monooxygenase 3D structures\|Monooxygenase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Faust, A]]		[[Category: Faust, A]]
	[[Category: Kubitza, C]]		[[Category: Kubitza, C]]

OCA at 06:26, 9 May 2018

OCA — Wed, 09 May 2018 06:26:10 GMT

←Older revision		Revision as of 06:26, 9 May 2018
Line 3:		Line 3:
	<StructureSection load='5nmw' size='340' side='right' caption='[[5nmw]], [[Resolution\|resolution]] 1.89Å' scene=''>		<StructureSection load='5nmw' size='340' side='right' caption='[[5nmw]], [[Resolution\|resolution]] 1.89Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5nmw]] is a 4 chain structure ~~with sequence from [http://en.wikipedia.org/wiki/Zonocerus_variegatus Zonocerus variegatus]~~. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NMW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5NMW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5nmw]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NMW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5NMW FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">pno ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=907066 Zonocerus variegatus])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5nmw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nmw OCA], [http://pdbe.org/5nmw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5nmw RCSB], [http://www.ebi.ac.uk/pdbsum/5nmw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5nmw ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5nmw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nmw OCA], [http://pdbe.org/5nmw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5nmw RCSB], [http://www.ebi.ac.uk/pdbsum/5nmw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5nmw ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The high-resolution crystal structure of the flavin-dependent monooxygenase (FMO) from the African locust Zonocerus variegatus is presented and the kinetics of structure-based protein variants are discussed. Z. variegatus expresses three flavin-dependent monooxygenase (ZvFMO) isoforms which contribute to a counterstrategy against pyrrolizidine alkaloids (PAs). PAs are protoxic compounds produced by some angiosperm lineages as a chemical defence against herbivores. N-Oxygenation of PAs and the accumulation of PA N-oxides within their haemolymph result in two evolutionary advantages for these insects: (i) they circumvent the defence mechanism of their food plants and (ii) they can use PA N-oxides to protect themselves against predators, which cannot cope with the toxic PAs. Despite a high degree of sequence identity and a similar substrate spectrum, the three ZvFMO isoforms differ greatly in enzyme activity. Here, the crystal structure of the Z. variegatus PA N-oxygenase (ZvPNO), the most active ZvFMO isoform, is reported at 1.6 A resolution together with kinetic studies of a second isoform, ZvFMOa. This is the first available crystal structure of an FMO from class B (of six different FMO subclasses, A-F) within the family of flavin-dependent monooxygenases that originates from a more highly developed organism than yeast. Despite the differences in sequence between family members, their overall structure is very similar. This indicates the need for high conservation of the three-dimensional structure for this type of reaction throughout all kingdoms of life. Nevertheless, this structure provides the closest relative to the human enzyme that is currently available for modelling studies. Of note, the crystal structure of ZvPNO reveals a unique dimeric arrangement as well as small conformational changes within the active site that have not been observed before. A newly observed kink within helix alpha8 close to the substrate-binding path might indicate a potential mechanism for product release. The data show that even single amino-acid exchanges in the substrate-entry path, rather than the binding site, have a significant impact on the specific enzyme activity of the isoforms.
		+
		+	Crystal structure of pyrrolizidine alkaloid N-oxygenase from the grasshopper Zonocerus variegatus.,Kubitza C, Faust A, Gutt M, Gath L, Ober D, Scheidig AJ Acta Crystallogr D Struct Biol. 2018 May 1;74(Pt 5):422-432. doi:, 10.1107/S2059798318003510. Epub 2018 Apr 24. PMID:29717713<ref>PMID:29717713</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5nmw" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Zonocerus variegatus]]
	[[Category: Faust, A]]		[[Category: Faust, A]]
	[[Category: Kubitza, C]]		[[Category: Kubitza, C]]

OCA at 07:00, 28 March 2018

OCA — Wed, 28 Mar 2018 07:00:20 GMT

←Older revision		Revision as of 07:00, 28 March 2018
Line 3:		Line 3:
	<StructureSection load='5nmw' size='340' side='right' caption='[[5nmw]], [[Resolution\|resolution]] 1.89Å' scene=''>		<StructureSection load='5nmw' size='340' side='right' caption='[[5nmw]], [[Resolution\|resolution]] 1.89Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5nmw]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NMW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5NMW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5nmw]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Zonocerus_variegatus Zonocerus variegatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NMW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5NMW FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">pno ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=907066 Zonocerus variegatus])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5nmw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nmw OCA], [http://pdbe.org/5nmw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5nmw RCSB], [http://www.ebi.ac.uk/pdbsum/5nmw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5nmw ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5nmw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nmw OCA], [http://pdbe.org/5nmw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5nmw RCSB], [http://www.ebi.ac.uk/pdbsum/5nmw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5nmw ProSAT]</span></td></tr>
	</table>		</table>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Zonocerus variegatus]]
	[[Category: Faust, A]]		[[Category: Faust, A]]
	[[Category: Kubitza, C]]		[[Category: Kubitza, C]]

OCA at 06:19, 14 March 2018

OCA — Wed, 14 Mar 2018 06:19:43 GMT

←Older revision		Revision as of 06:19, 14 March 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5nmw is ~~ON HOLD until Paper Publication~~	+	==Crystal Structure of the pyrrolizidine alkaloid N-oxygenase from Zonocerus variegatus in complex with FAD==
-		+	<StructureSection load='5nmw' size='340' side='right' caption='[[5nmw]], [[Resolution\|resolution]] 1.89Å' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5nmw]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NMW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5NMW FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5nmw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nmw OCA], [http://pdbe.org/5nmw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5nmw RCSB], [http://www.ebi.ac.uk/pdbsum/5nmw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5nmw ProSAT]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Faust, A]]
		+	[[Category: Kubitza, C]]
		+	[[Category: Ober, D]]
		+	[[Category: Scheidig, A]]
		+	[[Category: Fad]]
		+	[[Category: Flavin-containing monooxygenase]]
		+	[[Category: Oxidoreductase]]
		+	[[Category: Pyrrolizidine alkaloid n-oxygenase]]
		+	[[Category: Rossmann fold]]
		+	[[Category: Senecionine]]
		+	[[Category: Two dinucleotide binding domain flavoprotein]]

OCA: Protected "5nmw" [edit=sysop:move=sysop]

OCA — Wed, 19 Apr 2017 09:40:19 GMT

Protected "5nmw" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:40, 19 April 2017

OCA: New page: '''Unreleased structure''' The entry 5nmw is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

OCA — Wed, 19 Apr 2017 09:40:18 GMT

New page: '''Unreleased structure''' The entry 5nmw is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 5nmw is ON HOLD until Paper Publication

Authors:

Description:
[[Category: Unreleased Structures]]