5npo - Revision history

OCA at 13:20, 15 November 2023

OCA — Wed, 15 Nov 2023 13:20:21 GMT

←Older revision		Revision as of 13:20, 15 November 2023
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	<StructureSection load='5npo' size='340' side='right'caption='[[5npo]], [[Resolution\|resolution]] 1.95Å' scene=''>		<StructureSection load='5npo' size='340' side='right'caption='[[5npo]], [[Resolution\|resolution]] 1.95Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5npo]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NPO OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5NPO FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5npo]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NPO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5NPO FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.95Å</td></tr>
-	<tr id='~~activity~~'><td class="sblockLbl"><b>~~Activity~~:</b></td><td class="sblockDat"><~~span class~~='~~plainlinks~~'>~~[http://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6]~~ </~~span~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5npo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5npo OCA], [~~http~~://pdbe.org/5npo PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5npo RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5npo PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5npo ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5npo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5npo OCA], [https://pdbe.org/5npo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5npo RCSB], [https://www.ebi.ac.uk/pdbsum/5npo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5npo ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/BLAT_ECOLX BLAT_ECOLX]] TEM-type are the most prevalent beta-lactamases in enterobacteria; they hydrolyze the beta-lactam bond in susceptible beta-lactam antibiotics, thus conferring resistance to penicillins and cephalosporins. TEM-3 and TEM-4 are capable of hydrolyzing cefotaxime and ceftazidime. TEM-5 is capable of hydrolyzing ceftazidime. TEM-6 is capable of hydrolyzing ceftazidime and aztreonam. TEM-8/CAZ-2, TEM-16/CAZ-7 and TEM-24/CAZ-6 are markedly active against ceftazidime. IRT-4 shows resistance to beta-lactamase inhibitors.	+	[https://www.uniprot.org/uniprot/BLAT_ECOLX BLAT_ECOLX] TEM-type are the most prevalent beta-lactamases in enterobacteria; they hydrolyze the beta-lactam bond in susceptible beta-lactam antibiotics, thus conferring resistance to penicillins and cephalosporins. TEM-3 and TEM-4 are capable of hydrolyzing cefotaxime and ceftazidime. TEM-5 is capable of hydrolyzing ceftazidime. TEM-6 is capable of hydrolyzing ceftazidime and aztreonam. TEM-8/CAZ-2, TEM-16/CAZ-7 and TEM-24/CAZ-6 are markedly active against ceftazidime. IRT-4 shows resistance to beta-lactamase inhibitors.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Beta-lactamase~~]]	+	[[Category: Escherichia coli]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Cohen-Khait, R]]	+	[[Category: Cohen-Khait R]]
-	[[Category: Dym, O]]	+	[[Category: Dym O]]
-	[[Category: Hamer-Rogotner, S]]	+	[[Category: Hamer-Rogotner S]]
-	[[Category: Schreiber, G]]	+	[[Category: Schreiber G]]
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Tem1 beta-lactamase hetrodimer~~]]	+

OCA at 12:09, 26 August 2020

OCA — Wed, 26 Aug 2020 12:09:45 GMT

←Older revision		Revision as of 12:09, 26 August 2020
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	==Promiscuous Protein Self-Assembly as a Function of Protein Stability==		==Promiscuous Protein Self-Assembly as a Function of Protein Stability==
-	<StructureSection load='5npo' size='340' side='right' caption='[[5npo]], [[Resolution\|resolution]] 1.95Å' scene=''>	+	<StructureSection load='5npo' size='340' side='right'caption='[[5npo]], [[Resolution\|resolution]] 1.95Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5npo]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NPO OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5NPO FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5npo]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NPO OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5NPO FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5npo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5npo OCA], [http://pdbe.org/5npo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5npo RCSB], [http://www.ebi.ac.uk/pdbsum/5npo PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5npo ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5npo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5npo OCA], [http://pdbe.org/5npo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5npo RCSB], [http://www.ebi.ac.uk/pdbsum/5npo PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5npo ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	</div>		</div>
	<div class="pdbe-citations 5npo" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5npo" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Beta-lactamase 3D structures\|Beta-lactamase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
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	</StructureSection>		</StructureSection>
	[[Category: Beta-lactamase]]		[[Category: Beta-lactamase]]
		+	[[Category: Large Structures]]
	[[Category: Cohen-Khait, R]]		[[Category: Cohen-Khait, R]]
	[[Category: Dym, O]]		[[Category: Dym, O]]

OCA at 06:11, 20 December 2017

OCA — Wed, 20 Dec 2017 06:11:24 GMT

←Older revision		Revision as of 06:11, 20 December 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5npo is ~~ON HOLD until Paper~~ Publication	+	==Promiscuous Protein Self-Assembly as a Function of Protein Stability==
		+	<StructureSection load='5npo' size='340' side='right' caption='[[5npo]], [[Resolution\|resolution]] 1.95Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5npo]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NPO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5NPO FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5npo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5npo OCA], [http://pdbe.org/5npo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5npo RCSB], [http://www.ebi.ac.uk/pdbsum/5npo PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5npo ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/BLAT_ECOLX BLAT_ECOLX]] TEM-type are the most prevalent beta-lactamases in enterobacteria; they hydrolyze the beta-lactam bond in susceptible beta-lactam antibiotics, thus conferring resistance to penicillins and cephalosporins. TEM-3 and TEM-4 are capable of hydrolyzing cefotaxime and ceftazidime. TEM-5 is capable of hydrolyzing ceftazidime. TEM-6 is capable of hydrolyzing ceftazidime and aztreonam. TEM-8/CAZ-2, TEM-16/CAZ-7 and TEM-24/CAZ-6 are markedly active against ceftazidime. IRT-4 shows resistance to beta-lactamase inhibitors.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Proteins have evolved to balance efficient binding of desired partners with rejection of unwanted interactions. To investigate the evolution of protein-protein interactions, we selected a random library of pre-stabilized TEM1 beta-lactamase against wild-type TEM1 using yeast surface display. Three mutations were sufficient to achieve micromolar affinity binding between the two. The X-ray structure emphasized that the main contribution of the selected mutations was to modify the protein fold, specifically removing the N'-terminal helix, which consequently allowed protein coupling via a beta-sheet-mediated interaction resembling amyloid interaction mode. The only selected mutation located at the interaction interface (E58V) is reminiscent of the single mutation commonly causing sickle-cell anemia. Interestingly, the evolved mutations cannot be inserted into the wild-type protein due to reduced thermal stability of the resulting mutant protein. These results reveal a simple mechanism by which undesirable binding is purged by loss of thermal stability.

-	~~Authors~~:	+	Promiscuous Protein Binding as a Function of Protein Stability.,Cohen-Khait R, Dym O, Hamer-Rogotner S, Schreiber G Structure. 2017 Dec 5;25(12):1867-1874.e3. doi: 10.1016/j.str.2017.11.002. PMID:29211984<ref>PMID:29211984</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 5npo" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Beta-lactamase]]
		+	[[Category: Cohen-Khait, R]]
		+	[[Category: Dym, O]]
		+	[[Category: Hamer-Rogotner, S]]
		+	[[Category: Schreiber, G]]
		+	[[Category: Hydrolase]]
		+	[[Category: Tem1 beta-lactamase hetrodimer]]

OCA: Protected "5npo" [edit=sysop:move=sysop]

OCA — Thu, 27 Apr 2017 10:45:30 GMT

Protected "5npo" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:45, 27 April 2017

OCA: New page: '''Unreleased structure''' The entry 5npo is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

OCA — Thu, 27 Apr 2017 10:45:29 GMT

New page: '''Unreleased structure''' The entry 5npo is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 5npo is ON HOLD until Paper Publication

Authors:

Description:
[[Category: Unreleased Structures]]