5npt - Revision history

OCA at 07:27, 1 May 2024

OCA — Wed, 01 May 2024 07:27:26 GMT

←Older revision		Revision as of 07:27, 1 May 2024
Line 9:		Line 9:
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/R4IT35_9ASCO R4IT35_9ASCO]		[https://www.uniprot.org/uniprot/R4IT35_9ASCO R4IT35_9ASCO]
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The elongation of single-stranded DNA repeats at the 3'-ends of chromosomes by telomerase is a key process in maintaining genome integrity in eukaryotes. Abnormal activation of telomerase leads to uncontrolled cell division, whereas its down-regulation is attributed to ageing and several pathologies related to early cell death. Telomerase function is based on the dynamic interactions of its catalytic subunit (TERT) with nucleic acids-telomerase RNA, telomeric DNA and the DNA/RNA heteroduplex. Here, we present the crystallographic and NMR structures of the N-terminal (TEN) domain of TERT from the thermotolerant yeast Hansenula polymorpha and demonstrate the structural conservation of the core motif in evolutionarily divergent organisms. We identify the TEN residues that are involved in interactions with the telomerase RNA and in the recognition of the 'fork' at the distal end of the DNA product/RNA template heteroduplex. We propose that the TEN domain assists telomerase biological function and is involved in restricting the size of the heteroduplex during telomere repeat synthesis.
-
-	Structure and function of the N-terminal domain of the yeast telomerase reverse transcriptase.,Petrova OA, Mantsyzov AB, Rodina EV, Efimov SV, Hackenberg C, Hakanpaa J, Klochkov VV, Lebedev AA, Chugunova AA, Malyavko AN, Zatsepin TS, Mishin AV, Zvereva MI, Lamzin VS, Dontsova OA, Polshakov VI Nucleic Acids Res. 2017 Dec 23. pii: 4774275. doi: 10.1093/nar/gkx1275. PMID:29294091<ref>PMID:29294091</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 5npt" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Telomerase 3D structures\|Telomerase 3D structures]]		*[[Telomerase 3D structures\|Telomerase 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 13:20, 15 November 2023

OCA — Wed, 15 Nov 2023 13:20:49 GMT

←Older revision		Revision as of 13:20, 15 November 2023
Line 3:		Line 3:
	<StructureSection load='5npt' size='340' side='right'caption='[[5npt]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='5npt' size='340' side='right'caption='[[5npt]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5npt]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Atcc_34438 Atcc 34438~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NPT OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5NPT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5npt]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Ogataea_polymorpha Ogataea polymorpha]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NPT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5NPT FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5npt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5npt OCA], [~~http~~://pdbe.org/5npt PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5npt RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5npt PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5npt ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.4Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5npt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5npt OCA], [https://pdbe.org/5npt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5npt RCSB], [https://www.ebi.ac.uk/pdbsum/5npt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5npt ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/R4IT35_9ASCO R4IT35_9ASCO]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 17:		Line 20:

	==See Also==		==See Also==
-	*[[Telomerase\|Telomerase]]	+	*[[Telomerase 3D structures\|Telomerase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Atcc 34438]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Dontsova~~, O A~~]]	+	[[Category: Ogataea polymorpha]]
-	[[Category: Hackenberg, C]]	+	[[Category: Dontsova OA]]
-	[[Category: Hakanpaa, J]]	+	[[Category: Hackenberg C]]
-	[[Category: Lamzin~~, V S~~]]	+	[[Category: Hakanpaa J]]
-	[[Category: Lebedev~~, A A~~]]	+	[[Category: Lamzin VS]]
-	[[Category: Petrova~~, O A~~]]	+	[[Category: Lebedev AA]]
-	[[Category: Rodina~~, E V~~]]	+	[[Category: Petrova OA]]
-	[[Category: Zvereva~~, M I]]~~	+	[[Category: Rodina EV]]
-	~~[[Category: Telomerase]]~~	+	[[Category: Zvereva MI]]
-	~~[[Category: Transcription~~]]	+

OCA at 12:09, 26 August 2020

OCA — Wed, 26 Aug 2020 12:09:55 GMT

←Older revision		Revision as of 12:09, 26 August 2020
Line 1:		Line 1:

	==Structure of the N-terminal domain of the yeast telomerase reverse transcriptase==		==Structure of the N-terminal domain of the yeast telomerase reverse transcriptase==
-	<StructureSection load='5npt' size='340' side='right' caption='[[5npt]], [[Resolution\|resolution]] 2.40Å' scene=''>	+	<StructureSection load='5npt' size='340' side='right'caption='[[5npt]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5npt]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_34438 Atcc 34438]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NPT OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5NPT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5npt]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_34438 Atcc 34438]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NPT OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5NPT FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5npt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5npt OCA], [http://pdbe.org/5npt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5npt RCSB], [http://www.ebi.ac.uk/pdbsum/5npt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5npt ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5npt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5npt OCA], [http://pdbe.org/5npt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5npt RCSB], [http://www.ebi.ac.uk/pdbsum/5npt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5npt ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 15:		Line 15:
	</div>		</div>
	<div class="pdbe-citations 5npt" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5npt" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Telomerase\|Telomerase]]
	== References ==		== References ==
	<references/>		<references/>
Line 20:		Line 23:
	</StructureSection>		</StructureSection>
	[[Category: Atcc 34438]]		[[Category: Atcc 34438]]
		+	[[Category: Large Structures]]
	[[Category: Dontsova, O A]]		[[Category: Dontsova, O A]]
	[[Category: Hackenberg, C]]		[[Category: Hackenberg, C]]

OCA at 08:11, 17 January 2018

OCA — Wed, 17 Jan 2018 08:11:12 GMT

←Older revision		Revision as of 08:11, 17 January 2018
Line 3:		Line 3:
	<StructureSection load='5npt' size='340' side='right' caption='[[5npt]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='5npt' size='340' side='right' caption='[[5npt]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5npt]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NPT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5NPT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5npt]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_34438 Atcc 34438]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NPT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5NPT FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5npt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5npt OCA], [http://pdbe.org/5npt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5npt RCSB], [http://www.ebi.ac.uk/pdbsum/5npt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5npt ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5npt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5npt OCA], [http://pdbe.org/5npt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5npt RCSB], [http://www.ebi.ac.uk/pdbsum/5npt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5npt ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The elongation of single-stranded DNA repeats at the 3'-ends of chromosomes by telomerase is a key process in maintaining genome integrity in eukaryotes. Abnormal activation of telomerase leads to uncontrolled cell division, whereas its down-regulation is attributed to ageing and several pathologies related to early cell death. Telomerase function is based on the dynamic interactions of its catalytic subunit (TERT) with nucleic acids-telomerase RNA, telomeric DNA and the DNA/RNA heteroduplex. Here, we present the crystallographic and NMR structures of the N-terminal (TEN) domain of TERT from the thermotolerant yeast Hansenula polymorpha and demonstrate the structural conservation of the core motif in evolutionarily divergent organisms. We identify the TEN residues that are involved in interactions with the telomerase RNA and in the recognition of the 'fork' at the distal end of the DNA product/RNA template heteroduplex. We propose that the TEN domain assists telomerase biological function and is involved in restricting the size of the heteroduplex during telomere repeat synthesis.
		+
		+	Structure and function of the N-terminal domain of the yeast telomerase reverse transcriptase.,Petrova OA, Mantsyzov AB, Rodina EV, Efimov SV, Hackenberg C, Hakanpaa J, Klochkov VV, Lebedev AA, Chugunova AA, Malyavko AN, Zatsepin TS, Mishin AV, Zvereva MI, Lamzin VS, Dontsova OA, Polshakov VI Nucleic Acids Res. 2017 Dec 23. pii: 4774275. doi: 10.1093/nar/gkx1275. PMID:29294091<ref>PMID:29294091</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5npt" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Atcc 34438]]
	[[Category: Dontsova, O A]]		[[Category: Dontsova, O A]]
	[[Category: Hackenberg, C]]		[[Category: Hackenberg, C]]

OCA at 06:11, 20 December 2017

OCA — Wed, 20 Dec 2017 06:11:24 GMT

←Older revision		Revision as of 06:11, 20 December 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5npt ~~is ON HOLD until Paper Publication~~	+	==Structure of the N-terminal domain of the yeast telomerase reverse transcriptase==
-		+	<StructureSection load='5npt' size='340' side='right' caption='[[5npt]], [[Resolution\|resolution]] 2.40Å' scene=''>
-	~~Authors~~: ~~Rodina, E~~.V.~~, Lebedev, A~~.A.~~, Hakanpaa, J~~.~~, Hackenberg, C~~.~~, Petrova, O~~.A.~~, Zvereva, M~~.I.~~, Dontsova, O~~.A., ~~Lamzin, V~~.S.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5npt]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NPT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5NPT FirstGlance]. <br>
-	~~Description: Structure of the N~~-~~terminal domain of the yeast telomerase reverse transcriptase~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5npt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5npt OCA], [http://pdbe.org/5npt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5npt RCSB], [http://www.ebi.ac.uk/pdbsum/5npt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5npt ProSAT]</span></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[~~[Category~~: ~~Dontsova~~, O.A]]	+	__TOC__
-	[[Category: ~~Lebedev~~, A.A]]	+	</StructureSection>
		+	[[Category: Dontsova, O A]]
	[[Category: Hackenberg, C]]		[[Category: Hackenberg, C]]
	[[Category: Hakanpaa, J]]		[[Category: Hakanpaa, J]]
-	[[Category: ~~Zvereva~~, ~~M.I~~]]	+	[[Category: Lamzin, V S]]
-	[[Category: Rodina, E.V]]	+	[[Category: Lebedev, A A]]
-	[[Category: ~~Lamzin~~, ~~V.S~~]]	+	[[Category: Petrova, O A]]
-	[[Category: ~~Petrova, O.A~~]]	+	[[Category: Rodina, E V]]
		+	[[Category: Zvereva, M I]]
		+	[[Category: Telomerase]]
		+	[[Category: Transcription]]

OCA at 11:38, 27 September 2017

OCA — Wed, 27 Sep 2017 11:38:48 GMT

←Older revision		Revision as of 11:38, 27 September 2017
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5npt is ON HOLD	+	The entry 5npt is ON HOLD until Paper Publication

-	Authors: Rodina, E.V., Lebedev, A., Hakanpaa, J., Hackenberg, C., Petrova, O.A., Zvereva, M.I., Dontsova, O.A., Lamzin, V.S.	+	Authors: Rodina, E.V., Lebedev, A.A., Hakanpaa, J., Hackenberg, C., Petrova, O.A., Zvereva, M.I., Dontsova, O.A., Lamzin, V.S.

-	Description: Structure of the N-terminal domain of the telomerase reverse transcriptase ~~from~~	+	Description: Structure of the N-terminal domain of the yeast telomerase reverse transcriptase
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Dontsova, O.A]]		[[Category: Dontsova, O.A]]
-	[[Category: Lebedev, A]]	+	[[Category: Lebedev, A.A]]
	[[Category: Hackenberg, C]]		[[Category: Hackenberg, C]]
	[[Category: Hakanpaa, J]]		[[Category: Hakanpaa, J]]

OCA: Protected "5npt" [edit=sysop:move=sysop]

OCA — Thu, 27 Apr 2017 10:45:45 GMT

Protected "5npt" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:45, 27 April 2017

OCA: New page: '''Unreleased structure''' The entry 5npt is ON HOLD Authors: Rodina, E.V., Lebedev, A., Hakanpaa, J., Hackenberg, C., Petrova, O.A., Zvereva, M.I., Dontsova, O.A., Lamzin, V.S. Descri...

OCA — Thu, 27 Apr 2017 10:45:44 GMT

New page: '''Unreleased structure''' The entry 5npt is ON HOLD Authors: Rodina, E.V., Lebedev, A., Hakanpaa, J., Hackenberg, C., Petrova, O.A., Zvereva, M.I., Dontsova, O.A., Lamzin, V.S. Descri...

New page

'''Unreleased structure'''

The entry 5npt is ON HOLD

Authors: Rodina, E.V., Lebedev, A., Hakanpaa, J., Hackenberg, C., Petrova, O.A., Zvereva, M.I., Dontsova, O.A., Lamzin, V.S.

Description: Structure of the N-terminal domain of the telomerase reverse transcriptase from
[[Category: Unreleased Structures]]
[[Category: Dontsova, O.A]]
[[Category: Lebedev, A]]
[[Category: Hackenberg, C]]
[[Category: Hakanpaa, J]]
[[Category: Zvereva, M.I]]
[[Category: Rodina, E.V]]
[[Category: Lamzin, V.S]]
[[Category: Petrova, O.A]]