5nsq - Revision history

OCA at 12:07, 22 November 2023

OCA — Wed, 22 Nov 2023 12:07:04 GMT

←Older revision		Revision as of 12:07, 22 November 2023
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	==Structure of Leucyl aminopeptidase from Trypanosoma brucei in complex with Actinonin==		==Structure of Leucyl aminopeptidase from Trypanosoma brucei in complex with Actinonin==
-	<StructureSection load='5nsq' size='340' side='right' caption='[[5nsq]], [[Resolution\|resolution]] 3.00Å' scene=''>	+	<StructureSection load='5nsq' size='340' side='right'caption='[[5nsq]], [[Resolution\|resolution]] 3.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5nsq]] is a 6 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Tryb2 Tryb2~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NSQ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5NSQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5nsq]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Trypanosoma_brucei_brucei_TREU927 Trypanosoma brucei brucei TREU927]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NSQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5NSQ FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat"~~><scene name~~=~~'pdbligand=BB2:ACTINONIN'>BB2</scene~~>, ~~<scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3Å</td></tr>
-	<tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=~~UNK~~:~~UNKNOWN~~'>~~UNK~~</scene><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BB2:ACTINONIN'>BB2</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
-	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">Tb11.02.4440 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=185431 TRYB2])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5nsq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nsq OCA], [https://pdbe.org/5nsq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5nsq RCSB], [https://www.ebi.ac.uk/pdbsum/5nsq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5nsq ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5nsq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nsq OCA], [~~http~~://pdbe.org/5nsq PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5nsq RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5nsq PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5nsq ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q385B0_TRYB2 Q385B0_TRYB2]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Tryb2~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Timm, J~~]]	+	[[Category: Trypanosoma brucei brucei TREU927]]
-	[[Category: ~~Wilson, K~~]]	+	[[Category: Timm J]]
-	[[Category: ~~Aminopeptidase]]~~	+	[[Category: Wilson K]]
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: M17 leucyl aminopeptidase]]~~	+
-	~~[[Category: Tryanosoma brucei actinonin~~]]	+

OCA at 07:45, 22 February 2018

OCA — Thu, 22 Feb 2018 07:45:08 GMT

←Older revision		Revision as of 07:45, 22 February 2018
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	<StructureSection load='5nsq' size='340' side='right' caption='[[5nsq]], [[Resolution\|resolution]] 3.00Å' scene=''>		<StructureSection load='5nsq' size='340' side='right' caption='[[5nsq]], [[Resolution\|resolution]] 3.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5nsq]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NSQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5NSQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5nsq]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Tryb2 Tryb2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NSQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5NSQ FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BB2:ACTINONIN'>BB2</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BB2:ACTINONIN'>BB2</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Tb11.02.4440 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=185431 TRYB2])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5nsq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nsq OCA], [http://pdbe.org/5nsq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5nsq RCSB], [http://www.ebi.ac.uk/pdbsum/5nsq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5nsq ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5nsq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nsq OCA], [http://pdbe.org/5nsq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5nsq RCSB], [http://www.ebi.ac.uk/pdbsum/5nsq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5nsq ProSAT]</span></td></tr>
	</table>		</table>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Tryb2]]
	[[Category: Timm, J]]		[[Category: Timm, J]]
	[[Category: Wilson, K]]		[[Category: Wilson, K]]

OCA at 04:15, 30 August 2017

OCA — Wed, 30 Aug 2017 04:15:17 GMT

←Older revision		Revision as of 04:15, 30 August 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5nsq is ~~ON HOLD until Paper~~ Publication	+	==Structure of Leucyl aminopeptidase from Trypanosoma brucei in complex with Actinonin==
		+	<StructureSection load='5nsq' size='340' side='right' caption='[[5nsq]], [[Resolution\|resolution]] 3.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5nsq]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NSQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5NSQ FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BB2:ACTINONIN'>BB2</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5nsq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nsq OCA], [http://pdbe.org/5nsq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5nsq RCSB], [http://www.ebi.ac.uk/pdbsum/5nsq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5nsq ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Leucine aminopeptidase (LAP) is found in all kingdoms of life and catalyzes the metal-dependent hydrolysis of the N-terminal amino acid residue of peptide or amino acyl substrates. LAPs have been shown to participate in the N-terminal processing of certain proteins in mammalian cells and in homologous recombination and transcription regulation in bacteria, while in parasites, they are involved in host cell invasion and provision of essential amino acids for growth. The enzyme is essential for survival in Plasmodium falciparum, where its drug target potential has been suggested. We report here the X-ray structures of three kinetoplastid acidic LAPs (LAP-As from Trypanosoma brucei, Trypanosoma cruzi, and Leishmania major) which were solved in the metal-free and unliganded forms, as well as in a number of ligand complexes, providing insight into ligand binding, metal ion requirements, and oligomeric state. In addition, we analyzed mutant cells defective in LAP-A in Trypanosoma brucei, strongly suggesting that the enzyme is not required for the growth of this parasite either in vitro or in vivo. In procyclic cells, LAP-A was equally distributed throughout the cytoplasm, yet upon starvation, it relocalizes in particles that concentrate in the perinuclear region. Overexpression of the enzyme conferred a growth advantage when parasites were grown in leucine-deficient medium. Overall, the results suggest that in T. brucei, LAP-A may participate in protein degradation associated with nutrient depletion. IMPORTANCE Leucine aminopeptidases (LAPs) catalyze the hydrolysis of the N-terminal amino acid of peptides and are considered potential drug targets. They are involved in multiple functions ranging from host cell invasion and provision of essential amino acids to site-specific homologous recombination and transcription regulation. In kinetoplastid parasites, there are at least three distinct LAPs. The availability of the crystal structures provides important information for drug design. Here we report the structure of the acidic LAPs from three kinetoplastids in complex with different inhibitors and explore their role in Trypanosoma brucei survival under various nutrient conditions. Importantly, the acidic LAP is dispensable for growth both in vitro and in vivo, an observation that questions its use as a specific drug target. While LAP-A is not essential, leucine depletion and subcellular localization studies performed under starvation conditions suggest a possible function of LAP-A in the response to nutrient restriction.

-	~~Authors: Timm~~, J., Wilson, K.	+	Structural Characterization of Acidic M17 Leucine Aminopeptidases from the TriTryps and Evaluation of Their Role in Nutrient Starvation in Trypanosoma brucei.,Timm J, Valente M, Garcia-Caballero D, Wilson KS, Gonzalez-Pacanowska D mSphere. 2017 Aug 16;2(4). pii: e00226-17. doi: 10.1128/mSphere.00226-17., eCollection 2017 Jul-Aug. PMID:28815215<ref>PMID:28815215</ref>

-	~~Description: Structure~~ of ~~Leucyl aminopeptidase from Trypanosoma brucei in complex with Actinonin~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category~~: ~~Unreleased Structures]]~~	+	</div>
		+	<div class="pdbe-citations 5nsq" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Timm, J]]		[[Category: Timm, J]]
	[[Category: Wilson, K]]		[[Category: Wilson, K]]
		+	[[Category: Aminopeptidase]]
		+	[[Category: Hydrolase]]
		+	[[Category: M17 leucyl aminopeptidase]]
		+	[[Category: Tryanosoma brucei actinonin]]

OCA at 09:18, 10 May 2017

OCA — Wed, 10 May 2017 09:18:39 GMT

←Older revision		Revision as of 09:18, 10 May 2017
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5nsq is ON HOLD	+	The entry 5nsq is ON HOLD until Paper Publication

	Authors: Timm, J., Wilson, K.		Authors: Timm, J., Wilson, K.

OCA: Protected "5nsq" [edit=sysop:move=sysop]

OCA — Thu, 04 May 2017 09:40:54 GMT

Protected "5nsq" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:40, 4 May 2017

OCA: New page: '''Unreleased structure''' The entry 5nsq is ON HOLD Authors: Timm, J., Wilson, K. Description: Structure of Leucyl aminopeptidase from Trypanosoma brucei in complex with Actinonin [[C...

OCA — Thu, 04 May 2017 09:40:53 GMT

New page: '''Unreleased structure''' The entry 5nsq is ON HOLD Authors: Timm, J., Wilson, K. Description: Structure of Leucyl aminopeptidase from Trypanosoma brucei in complex with Actinonin [[C...

New page

'''Unreleased structure'''

The entry 5nsq is ON HOLD

Authors: Timm, J., Wilson, K.

Description: Structure of Leucyl aminopeptidase from Trypanosoma brucei in complex with Actinonin
[[Category: Unreleased Structures]]
[[Category: Timm, J]]
[[Category: Wilson, K]]