5nuk - Revision history

OCA at 11:50, 1 February 2024

OCA — Thu, 01 Feb 2024 11:50:41 GMT

←Older revision		Revision as of 11:50, 1 February 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5nuk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NUK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5NUK FirstGlance]. <br>		<table><tr><td colspan='2'>[[5nuk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NUK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5NUK FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=Z80:3-(2-CHLORO-10H-PHENOTHIAZIN-10-YL)-N,N-DIMETHYLPROPAN-1-AMINE'>Z80</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.7Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=Z80:3-(2-CHLORO-10H-PHENOTHIAZIN-10-YL)-N,N-DIMETHYLPROPAN-1-AMINE'>Z80</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5nuk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nuk OCA], [https://pdbe.org/5nuk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5nuk RCSB], [https://www.ebi.ac.uk/pdbsum/5nuk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5nuk ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5nuk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nuk OCA], [https://pdbe.org/5nuk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5nuk RCSB], [https://www.ebi.ac.uk/pdbsum/5nuk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5nuk ProSAT]</span></td></tr>
	</table>		</table>

OCA at 08:25, 7 December 2022

OCA — Wed, 07 Dec 2022 08:25:15 GMT

←Older revision		Revision as of 08:25, 7 December 2022
Line 1:		Line 1:

	==Engineered beta-lactoglobulin: variant I56F-L39A-M107F in complex with chlorpromazine (LG-FAF-CLP)==		==Engineered beta-lactoglobulin: variant I56F-L39A-M107F in complex with chlorpromazine (LG-FAF-CLP)==
-	<StructureSection load='5nuk' size='340' side='right' caption='[[5nuk]], [[Resolution\|resolution]] 1.70Å' scene=''>	+	<StructureSection load='5nuk' size='340' side='right'caption='[[5nuk]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5nuk]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Bovin Bovin~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NUK OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5NUK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5nuk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NUK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5NUK FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=Z80:3-(2-CHLORO-10H-PHENOTHIAZIN-10-YL)-N,N-DIMETHYLPROPAN-1-AMINE'>Z80</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=Z80:3-(2-CHLORO-10H-PHENOTHIAZIN-10-YL)-N,N-DIMETHYLPROPAN-1-AMINE'>Z80</scene></td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">LGB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9913 BOVIN])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5nuk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nuk OCA], [https://pdbe.org/5nuk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5nuk RCSB], [https://www.ebi.ac.uk/pdbsum/5nuk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5nuk ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5nuk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nuk OCA], [~~http~~://pdbe.org/5nuk PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5nuk RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5nuk PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5nuk ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/LACB_BOVIN LACB_BOVIN]] Primary component of whey, it binds retinol and is probably involved in the transport of that molecule.	+	[https://www.uniprot.org/uniprot/LACB_BOVIN LACB_BOVIN] Primary component of whey, it binds retinol and is probably involved in the transport of that molecule.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 19:		Line 18:
	</div>		</div>
	<div class="pdbe-citations 5nuk" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5nuk" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Beta-lactoglobulin 3D structures\|Beta-lactoglobulin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bovin~~]]	+	[[Category: Bos taurus]]
-	[[Category: Bonarek, P]]	+	[[Category: Large Structures]]
-	[[Category: Lazinska, I]]	+	[[Category: Bonarek P]]
-	[[Category: Lewinski, K]]	+	[[Category: Lazinska I]]
-	[[Category: Loch~~, J I~~]]	+	[[Category: Lewinski K]]
-	[[Category: Szydlowska, J]]	+	[[Category: Loch JI]]
-	[[Category: Tworzydlo, M]]	+	[[Category: Szydlowska J]]
-	~~[[Category: Beta-lactoglobulin]]~~	+	[[Category: Tworzydlo M]]
-	~~[[Category: Chlorpromazine]]~~	+
-	~~[[Category: Complex]]~~	+
-	~~[[Category: Lipocalin]]~~	+
-	~~[[Category: Mutation]]~~	+
-	~~[[Category: Transport protein~~]]	+

OCA at 06:27, 18 April 2018

OCA — Wed, 18 Apr 2018 06:27:13 GMT

←Older revision		Revision as of 06:27, 18 April 2018
Line 3:		Line 3:
	<StructureSection load='5nuk' size='340' side='right' caption='[[5nuk]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='5nuk' size='340' side='right' caption='[[5nuk]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5nuk]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NUK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5NUK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5nuk]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bovin Bovin]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NUK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5NUK FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=Z80:3-(2-CHLORO-10H-PHENOTHIAZIN-10-YL)-N,N-DIMETHYLPROPAN-1-AMINE'>Z80</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=Z80:3-(2-CHLORO-10H-PHENOTHIAZIN-10-YL)-N,N-DIMETHYLPROPAN-1-AMINE'>Z80</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">LGB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9913 BOVIN])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5nuk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nuk OCA], [http://pdbe.org/5nuk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5nuk RCSB], [http://www.ebi.ac.uk/pdbsum/5nuk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5nuk ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5nuk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nuk OCA], [http://pdbe.org/5nuk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5nuk RCSB], [http://www.ebi.ac.uk/pdbsum/5nuk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5nuk ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/LACB_BOVIN LACB_BOVIN]] Primary component of whey, it binds retinol and is probably involved in the transport of that molecule.		[[http://www.uniprot.org/uniprot/LACB_BOVIN LACB_BOVIN]] Primary component of whey, it binds retinol and is probably involved in the transport of that molecule.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Chlorpromazine (CPZ) is a phenothiazine acting as dopamine antagonist. Aside from application in schizophrenia therapy, chlorpromazine is found to be a putative inhibitor of proteins involved in cancers, heritable autism disorder and prion diseases. Four new beta-lactoglobulin variants with double or triple substitutions: I56F/L39A, F105L/L39A, I56F/L39A/M107F or F105L/L39A/M107F changing the shape of the binding pocket were produced and their chlorpromazine binding properties have been investigated by X-ray crystallography, circular dichroism, isothermal titration calorimetry and thermophoresis. The CD spectra and crystal structures revealed that mutations do not affect the protein overall structure but in comparison to WT protein, variants possessing I56F substitution had lower stability while mutation F105L increased melting temperature of the protein. The new variants showed affinity to chlorpromazine in the range 4.2-15.4x10(3)M(-1). The CD spectra and crystal structures revealed complementarity of the binding pocket shape, to only one chlorpromazine chiral conformer. The (aR)-CPZ was bonded to variants containing I56F substitution while variants with F105L substitution preferred (aS)-CPZ.
		+
		+	The engineered beta-lactoglobulin with complementarity to the chlorpromazine chiral conformers.,Loch JI, Bonarek P, Tworzydlo M, Lazinska I, Szydlowska J, Lipowska J, Rzesikowska K, Lewinski K Int J Biol Macromol. 2018 Mar 16;114:85-96. doi: 10.1016/j.ijbiomac.2018.03.074. PMID:29555509<ref>PMID:29555509</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5nuk" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bovin]]
	[[Category: Bonarek, P]]		[[Category: Bonarek, P]]
	[[Category: Lazinska, I]]		[[Category: Lazinska, I]]

OCA at 05:51, 4 April 2018

OCA — Wed, 04 Apr 2018 05:51:40 GMT

←Older revision		Revision as of 05:51, 4 April 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry 5nuk is ON HOLD until Paper Publication~~	+	==Engineered beta-lactoglobulin: variant I56F-L39A-M107F in complex with chlorpromazine (LG-FAF-CLP)==
-		+	<StructureSection load='5nuk' size='340' side='right' caption='[[5nuk]], [[Resolution\|resolution]] 1.70Å' scene=''>
-	~~Authors: Loch, J.I., Bonarek, P., Tworzydlo, M., Lazinska, I., Szydlowska, J., Lewinski, K.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5nuk]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NUK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5NUK FirstGlance]. <br>
-	~~Description:~~ Engineered beta-lactoglobulin: variant I56F-L39A-M107F in complex with chlorpromazine (LG-FAF-CLP)	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=Z80:3-(2-CHLORO-10H-PHENOTHIAZIN-10-YL)-N,N-DIMETHYLPROPAN-1-AMINE'>Z80</scene></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5nuk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nuk OCA], [http://pdbe.org/5nuk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5nuk RCSB], [http://www.ebi.ac.uk/pdbsum/5nuk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5nuk ProSAT]</span></td></tr>
-	[[~~Category~~: ~~Loch~~, J.I]]	+	</table>
-	[[Category: ~~Tworzydlo~~, M]]	+	== Function ==
		+	[[http://www.uniprot.org/uniprot/LACB_BOVIN LACB_BOVIN]] Primary component of whey, it binds retinol and is probably involved in the transport of that molecule.
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Bonarek, P]]
		+	[[Category: Lazinska, I]]
	[[Category: Lewinski, K]]		[[Category: Lewinski, K]]
-	[[Category: ~~Lazinska~~, I]]	+	[[Category: Loch, J I]]
-	~~[[Category: Bonarek, P~~]]	+
	[[Category: Szydlowska, J]]		[[Category: Szydlowska, J]]
		+	[[Category: Tworzydlo, M]]
		+	[[Category: Beta-lactoglobulin]]
		+	[[Category: Chlorpromazine]]
		+	[[Category: Complex]]
		+	[[Category: Lipocalin]]
		+	[[Category: Mutation]]
		+	[[Category: Transport protein]]

OCA: Protected "5nuk" [edit=sysop:move=sysop]

OCA — Wed, 10 May 2017 09:22:10 GMT

Protected "5nuk" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:22, 10 May 2017

OCA: New page: '''Unreleased structure''' The entry 5nuk is ON HOLD until Paper Publication Authors: Loch, J.I., Bonarek, P., Tworzydlo, M., Lazinska, I., Szydlowska, J., Lewinski, K. Description: En...

OCA — Wed, 10 May 2017 09:22:09 GMT

New page: '''Unreleased structure''' The entry 5nuk is ON HOLD until Paper Publication Authors: Loch, J.I., Bonarek, P., Tworzydlo, M., Lazinska, I., Szydlowska, J., Lewinski, K. Description: En...

New page

'''Unreleased structure'''

The entry 5nuk is ON HOLD until Paper Publication

Authors: Loch, J.I., Bonarek, P., Tworzydlo, M., Lazinska, I., Szydlowska, J., Lewinski, K.

Description: Engineered beta-lactoglobulin: variant I56F-L39A-M107F in complex with chlorpromazine (LG-FAF-CLP)
[[Category: Unreleased Structures]]
[[Category: Loch, J.I]]
[[Category: Tworzydlo, M]]
[[Category: Lewinski, K]]
[[Category: Lazinska, I]]
[[Category: Bonarek, P]]
[[Category: Szydlowska, J]]