5nxs - Revision history

OCA at 12:15, 22 November 2023

OCA — Wed, 22 Nov 2023 12:15:34 GMT

←Older revision		Revision as of 12:15, 22 November 2023
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	==Crystal Structure of Human Pro-myostatin Precursor at 4.2 A Resolution with Experimental Phases from SeMet labelling==		==Crystal Structure of Human Pro-myostatin Precursor at 4.2 A Resolution with Experimental Phases from SeMet labelling==
-	<StructureSection load='5nxs' size='340' side='right' caption='[[5nxs]], [[Resolution\|resolution]] 4.19Å' scene=''>	+	<StructureSection load='5nxs' size='340' side='right'caption='[[5nxs]], [[Resolution\|resolution]] 4.19Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5nxs]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NXS OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5NXS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5nxs]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NXS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5NXS FirstGlance]. <br>
-	</td></tr><tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"~~><scene name~~=~~'pdbligand=MSE:SELENOMETHIONINE'>MSE</scene~~></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 4.19Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~MSTN, GDF8 ([http~~:/~~/www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5nxs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nxs OCA], [~~http~~://pdbe.org/5nxs PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5nxs RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5nxs PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5nxs ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5nxs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nxs OCA], [https://pdbe.org/5nxs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5nxs RCSB], [https://www.ebi.ac.uk/pdbsum/5nxs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5nxs ProSAT]</span></td></tr>
	</table>		</table>
	== Disease ==		== Disease ==
-	[~~[http~~://www.uniprot.org/uniprot/GDF8_HUMAN GDF8_HUMAN]] Myostatin-related muscle hypertrophy. The disease is caused by mutations affecting the gene represented in this entry.	+	[https://www.uniprot.org/uniprot/GDF8_HUMAN GDF8_HUMAN] Myostatin-related muscle hypertrophy. The disease is caused by mutations affecting the gene represented in this entry.
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/GDF8_HUMAN GDF8_HUMAN]] Acts specifically as a negative regulator of skeletal muscle growth.	+	[https://www.uniprot.org/uniprot/GDF8_HUMAN GDF8_HUMAN] Acts specifically as a negative regulator of skeletal muscle growth.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 5nxs" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5nxs" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Growth differentiation factor 3D STRUCTURES\|Growth differentiation factor 3D STRUCTURES]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
-	[[Category: ~~Cotton, T R~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Fischer, G~~]]	+	[[Category: Cotton TR]]
-	[[Category: ~~Hyvonen, M~~]]	+	[[Category: Fischer G]]
-	[[Category: ~~Cystine knot]]~~	+	[[Category: Hyvonen M]]
-	~~[[Category: Growth factor]]~~	+
-	~~[[Category: Signaling protein]]~~	+
-	~~[[Category: Tgfbeta family~~]]	+

OCA at 20:43, 24 January 2018

OCA — Wed, 24 Jan 2018 20:43:23 GMT

←Older revision		Revision as of 20:43, 24 January 2018
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	<StructureSection load='5nxs' size='340' side='right' caption='[[5nxs]], [[Resolution\|resolution]] 4.19Å' scene=''>		<StructureSection load='5nxs' size='340' side='right' caption='[[5nxs]], [[Resolution\|resolution]] 4.19Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5nxs]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NXS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5NXS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5nxs]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NXS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5NXS FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MSTN, GDF8 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5nxs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nxs OCA], [http://pdbe.org/5nxs PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5nxs RCSB], [http://www.ebi.ac.uk/pdbsum/5nxs PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5nxs ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5nxs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nxs OCA], [http://pdbe.org/5nxs PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5nxs RCSB], [http://www.ebi.ac.uk/pdbsum/5nxs PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5nxs ProSAT]</span></td></tr>
	</table>		</table>
Line 11:		Line 12:
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/GDF8_HUMAN GDF8_HUMAN]] Acts specifically as a negative regulator of skeletal muscle growth.		[[http://www.uniprot.org/uniprot/GDF8_HUMAN GDF8_HUMAN]] Acts specifically as a negative regulator of skeletal muscle growth.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Myostatin, a key regulator of muscle mass in vertebrates, is biosynthesised as a latent precursor in muscle and is activated by sequential proteolysis of the pro-domain. To investigate the molecular mechanism by which pro-myostatin remains latent, we have determined the structure of unprocessed pro-myostatin and analysed the properties of the protein in its different forms. Crystal structures and SAXS analyses show that pro-myostatin adopts an open, V-shaped structure with a domain-swapped arrangement. The pro-mature complex, after cleavage of the furin site, has significantly reduced activity compared with the mature growth factor and persists as a stable complex that is resistant to the natural antagonist follistatin. The latency appears to be conferred by a number of distinct features that collectively stabilise the interaction of the pro-domains with the mature growth factor, enabling a regulated stepwise activation process, distinct from the prototypical pro-TGF-beta1. These results provide a basis for understanding the effect of missense mutations in pro-myostatin and pave the way for the design of novel myostatin inhibitors.
		+
		+	Structure of the human myostatin precursor and determinants of growth factor latency.,Cotton TR, Fischer G, Wang X, McCoy JC, Czepnik M, Thompson TB, Hyvonen M EMBO J. 2018 Jan 12. pii: embj.201797883. doi: 10.15252/embj.201797883. PMID:29330193<ref>PMID:29330193</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5nxs" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
	[[Category: Cotton, T R]]		[[Category: Cotton, T R]]
	[[Category: Fischer, G]]		[[Category: Fischer, G]]

OCA at 06:49, 17 January 2018

OCA — Wed, 17 Jan 2018 06:49:59 GMT

←Older revision		Revision as of 06:49, 17 January 2018
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-	'''Unreleased structure'''

-	~~The entry~~ 5nxs is ~~ON HOLD until Paper Publication~~	+	==Crystal Structure of Human Pro-myostatin Precursor at 4.2 A Resolution with Experimental Phases from SeMet labelling==
-		+	<StructureSection load='5nxs' size='340' side='right' caption='[[5nxs]], [[Resolution\|resolution]] 4.19Å' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5nxs]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NXS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5NXS FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5nxs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nxs OCA], [http://pdbe.org/5nxs PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5nxs RCSB], [http://www.ebi.ac.uk/pdbsum/5nxs PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5nxs ProSAT]</span></td></tr>
		+	</table>
		+	== Disease ==
		+	[[http://www.uniprot.org/uniprot/GDF8_HUMAN GDF8_HUMAN]] Myostatin-related muscle hypertrophy. The disease is caused by mutations affecting the gene represented in this entry.
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/GDF8_HUMAN GDF8_HUMAN]] Acts specifically as a negative regulator of skeletal muscle growth.
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Cotton, T R]]
		+	[[Category: Fischer, G]]
		+	[[Category: Hyvonen, M]]
		+	[[Category: Cystine knot]]
		+	[[Category: Growth factor]]
		+	[[Category: Signaling protein]]
		+	[[Category: Tgfbeta family]]

OCA at 11:50, 27 September 2017

OCA — Wed, 27 Sep 2017 11:50:51 GMT

←Older revision		Revision as of 11:50, 27 September 2017
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5nxs is ON HOLD	+	The entry 5nxs is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "5nxs" [edit=sysop:move=sysop]

OCA — Wed, 17 May 2017 12:24:30 GMT

Protected "5nxs" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:24, 17 May 2017

OCA: New page: '''Unreleased structure''' The entry 5nxs is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 17 May 2017 12:24:29 GMT

New page: '''Unreleased structure''' The entry 5nxs is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 5nxs is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]