5o14 - Revision history

OCA at 12:20, 22 November 2023

OCA — Wed, 22 Nov 2023 12:20:43 GMT

←Older revision		Revision as of 12:20, 22 November 2023
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	==Co-crystal structure of a cross-reactive bactericidal human antibody targeting meningococcal vaccine antigen factor H binding protein==		==Co-crystal structure of a cross-reactive bactericidal human antibody targeting meningococcal vaccine antigen factor H binding protein==
-	<StructureSection load='5o14' size='340' side='right' caption='[[5o14]], [[Resolution\|resolution]] 2.19Å' scene=''>	+	<StructureSection load='5o14' size='340' side='right'caption='[[5o14]], [[Resolution\|resolution]] 2.19Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5o14]] is a 6 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~] and [~~http~~://en.wikipedia.org/wiki/~~Neimb Neimb~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5O14 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5O14 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5o14]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Neisseria_meningitidis_MC58 Neisseria meningitidis MC58]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5O14 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5O14 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat"~~><scene name~~=~~'pdbligand=CL:CHLORIDE+ION'>CL</scene~~>, ~~<scene name='pdbligand=EDO:1,~~2~~-ETHANEDIOL'>EDO</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.195Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~NMB1870 ([http~~:/~~/www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=~~5&id=122586 NEIMB])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5o14 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5o14 OCA], [~~http~~://pdbe.org/5o14 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5o14 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5o14 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5o14 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5o14 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5o14 OCA], [https://pdbe.org/5o14 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5o14 RCSB], [https://www.ebi.ac.uk/pdbsum/5o14 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5o14 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/FHBP_NEIMB FHBP_NEIMB] A bacterial surface lipoprotein that binds host (human) complement factor H (fH, gene CFH), binding contributes to the avoidance of complement-mediated lysis by N.meningitidis. Binding of fH to the bacteria surface is independent of bacterial sialic acid moieties (PubMed:16751403). fH binding affinity is high enough that it may sequester plasma fH, depleting its circulating levels and de-regulating complement in the host (Probable). This protein induces high levels of bactericidal antibodies in mice (PubMed:12642606, PubMed:15039331, PubMed:15664958, PubMed:21753121, PubMed:23133374).<ref>PMID:12642606</ref> <ref>PMID:15039331</ref> <ref>PMID:15664958</ref> <ref>PMID:16751403</ref> <ref>PMID:21753121</ref> <ref>PMID:23133374</ref> <ref>PMID:19225461</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
-	[[Category: ~~Neimb~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Lopez-Sagaseta, J~~]]	+	[[Category: Neisseria meningitidis MC58]]
-	[[Category: ~~Factor h binding protein]]~~	+	[[Category: Lopez-Sagaseta J]]
-	~~[[Category: Human fab]]~~	+
-	~~[[Category: Immune system]]~~	+
-	~~[[Category: Infection]]~~	+
-	~~[[Category: Outer membrane~~]]	+

OCA at 07:45, 22 February 2018

OCA — Thu, 22 Feb 2018 07:45:39 GMT

←Older revision		Revision as of 07:45, 22 February 2018
Line 3:		Line 3:
	<StructureSection load='5o14' size='340' side='right' caption='[[5o14]], [[Resolution\|resolution]] 2.19Å' scene=''>		<StructureSection load='5o14' size='340' side='right' caption='[[5o14]], [[Resolution\|resolution]] 2.19Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5o14]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5O14 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5O14 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5o14]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human] and [http://en.wikipedia.org/wiki/Neimb Neimb]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5O14 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5O14 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">NMB1870 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=122586 NEIMB])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5o14 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5o14 OCA], [http://pdbe.org/5o14 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5o14 RCSB], [http://www.ebi.ac.uk/pdbsum/5o14 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5o14 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5o14 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5o14 OCA], [http://pdbe.org/5o14 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5o14 RCSB], [http://www.ebi.ac.uk/pdbsum/5o14 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5o14 ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Data obtained recently in the United Kingdom following a nationwide infant immunization program against serogroup B Neisseria meningitidis (MenB) reported >80% 4CMenB vaccine-mediated protection. Factor H-binding protein (fHbp) is a meningococcal virulence factor and a component of two new MenB vaccines. Here, we investigated the structural bases underlying the fHbp-dependent protective antibody response in humans, which might inform future antigen design efforts. We present the co-crystal structure of a human antibody Fab targeting fHbp. The vaccine-elicited Fab 1A12 is cross-reactive and targets an epitope highly conserved across the repertoire of three naturally occurring fHbp variants. The free Fab structure highlights conformational rearrangements occurring upon antigen binding. Importantly, 1A12 is bactericidal against MenB strains expressing fHbp from all three variants. Our results reveal important immunological features potentially contributing to the broad protection conferred by fHbp vaccination. Our studies fuel the rationale of presenting conserved protein epitopes when developing broadly protective vaccines.
		+
		+	Crystal structure reveals vaccine elicited bactericidal human antibody targeting a conserved epitope on meningococcal fHbp.,Lopez-Sagaseta J, Beernink PT, Bianchi F, Santini L, Frigimelica E, Lucas AH, Pizza M, Bottomley MJ Nat Commun. 2018 Feb 6;9(1):528. doi: 10.1038/s41467-018-02827-7. PMID:29410413<ref>PMID:29410413</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5o14" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
		+	[[Category: Neimb]]
	[[Category: Lopez-Sagaseta, J]]		[[Category: Lopez-Sagaseta, J]]
	[[Category: Factor h binding protein]]		[[Category: Factor h binding protein]]

OCA at 06:24, 15 February 2018

OCA — Thu, 15 Feb 2018 06:24:50 GMT

←Older revision		Revision as of 06:24, 15 February 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5o14 is ~~ON HOLD until Paper Publication~~	+	==Co-crystal structure of a cross-reactive bactericidal human antibody targeting meningococcal vaccine antigen factor H binding protein==
-		+	<StructureSection load='5o14' size='340' side='right' caption='[[5o14]], [[Resolution\|resolution]] 2.19Å' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5o14]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5O14 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5O14 FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5o14 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5o14 OCA], [http://pdbe.org/5o14 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5o14 RCSB], [http://www.ebi.ac.uk/pdbsum/5o14 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5o14 ProSAT]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Lopez-Sagaseta, J]]
		+	[[Category: Factor h binding protein]]
		+	[[Category: Human fab]]
		+	[[Category: Immune system]]
		+	[[Category: Infection]]
		+	[[Category: Outer membrane]]

OCA at 11:54, 27 September 2017

OCA — Wed, 27 Sep 2017 11:54:17 GMT

←Older revision		Revision as of 11:54, 27 September 2017
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5o14 is ON HOLD	+	The entry 5o14 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "5o14" [edit=sysop:move=sysop]

OCA — Wed, 24 May 2017 10:07:12 GMT

Protected "5o14" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:07, 24 May 2017

OCA: New page: '''Unreleased structure''' The entry 5o14 is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 24 May 2017 10:07:11 GMT

New page: '''Unreleased structure''' The entry 5o14 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 5o14 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]