5o1v - Revision history

OCA at 19:04, 29 November 2023

OCA — Wed, 29 Nov 2023 19:04:02 GMT

←Older revision		Revision as of 19:04, 29 November 2023
Line 3:		Line 3:
	<StructureSection load='5o1v' size='340' side='right'caption='[[5o1v]], [[Resolution\|resolution]] 1.72Å' scene=''>		<StructureSection load='5o1v' size='340' side='right'caption='[[5o1v]], [[Resolution\|resolution]] 1.72Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5o1v]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5O1V OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5O1V FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5o1v]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5O1V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5O1V FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat"~~><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.723Å</td></tr>
-	~~<tr~~ id~~='NonStdRes'><td class~~="~~sblockLbl~~">~~<b>~~[[~~Non-Standard_Residue~~\|~~NonStd Res:~~]]~~</b></td><td class="sblockDat"><scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene>~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene></td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>WNK3, KIAA1566, PRKWNK3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~9606 HUMAN])</td~~><~~/tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5o1v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5o1v OCA], [https://pdbe.org/5o1v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5o1v RCSB], [https://www.ebi.ac.uk/pdbsum/5o1v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5o1v ProSAT]</span></td></tr>
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b~~></td><~~td class~~=~~"sblockDat"><span class~~=~~'plainlinks~~'>[http://en.wikipedia.org/wiki/Non-specific_serine/threonine_protein_kinase Non-specific serine/threonine protein kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.1 2.7.11.1] </~~span~~></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5o1v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5o1v OCA], [~~http~~://pdbe.org/5o1v PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5o1v RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5o1v PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5o1v ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/WNK3_HUMAN WNK3_HUMAN]] Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively (PubMed:16275913, PubMed:16275911, PubMed:16357011). Phosphorylates WNK4. Regulates the phosphorylation of SLC12A1 and SLC12A2. Increases Ca(2+) influx mediated by TRPV5 and TRPV6 by enhancing their membrane expression level via a kinase-dependent pathway (PubMed:18768590). Inhibits the activity of KCNJ1 by decreasing its expression at the cell membrane in a non-catalytic manner.<ref>PMID:16275911</ref> <ref>PMID:16275913</ref> <ref>PMID:16357011</ref> <ref>PMID:16501604</ref> <ref>PMID:17975670</ref> <ref>PMID:18768590</ref> <ref>PMID:20525693</ref> Isoform 1, isoform 2, isoform 3 and isoform 4 stimulate the activity of SLC12A1, SLC12A2 and SLC12A3 and inhibit the activity of SLC12A4, SLC12A5, SLC12A6 and SLC12A7. According to PubMed:19470686, isoform 1 inhibits the activity of SLC12A3.<ref>PMID:19470686</ref> <ref>PMID:21613606</ref>	+	[https://www.uniprot.org/uniprot/WNK3_HUMAN WNK3_HUMAN] Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively (PubMed:16275913, PubMed:16275911, PubMed:16357011). Phosphorylates WNK4. Regulates the phosphorylation of SLC12A1 and SLC12A2. Increases Ca(2+) influx mediated by TRPV5 and TRPV6 by enhancing their membrane expression level via a kinase-dependent pathway (PubMed:18768590). Inhibits the activity of KCNJ1 by decreasing its expression at the cell membrane in a non-catalytic manner.<ref>PMID:16275911</ref> <ref>PMID:16275913</ref> <ref>PMID:16357011</ref> <ref>PMID:16501604</ref> <ref>PMID:17975670</ref> <ref>PMID:18768590</ref> <ref>PMID:20525693</ref> Isoform 1, isoform 2, isoform 3 and isoform 4 stimulate the activity of SLC12A1, SLC12A2 and SLC12A3 and inhibit the activity of SLC12A4, SLC12A5, SLC12A6 and SLC12A7. According to PubMed:19470686, isoform 1 inhibits the activity of SLC12A3.<ref>PMID:19470686</ref> <ref>PMID:21613606</ref>

	==See Also==		==See Also==
-	*[[Serine/threonine protein kinase\|Serine/threonine protein kinase]]	+	*[[Serine/threonine protein kinase 3D structures\|Serine/threonine protein kinase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: Non-specific serine/threonine protein kinase]]~~	+	[[Category: Arrowsmith CH]]
-	[[Category: Arrowsmith~~, C H~~]]	+	[[Category: Berridge G]]
-	[[Category: Berridge, G]]	+	[[Category: Bountra C]]
-	[[Category: Bountra, C]]	+	[[Category: Bufton JC]]
-	[[Category: Bufton~~, J C~~]]	+	[[Category: Bullock A]]
-	[[Category: Bullock, A]]	+	[[Category: Burgess-Brown NA]]
-	[[Category: Burgess-Brown~~, N A~~]]	+	[[Category: Chalk R]]
-	[[Category: Chalk, R]]	+	[[Category: Dixon-Clarke SE]]
-	~~[[Category: Delft, F von~~]]	+	[[Category: Edwards AM]]
-	[[Category: Dixon-Clarke~~, S E~~]]	+	[[Category: Fairhead M]]
-	[[Category: Edwards~~, A M~~]]	+	[[Category: Kopec J]]
-	[[Category: Fairhead, M]]	+	[[Category: Kupinska K]]
-	[[Category: Kopec, J]]	+	[[Category: Pinkas DM]]
-	[[Category: Kupinska, K]]	+	[[Category: Sethi R]]
-	[[Category: Pinkas~~, D M]]~~	+	[[Category: Wang D]]
-	~~[[Category: Structural genomic~~]]	+	[[Category: Von Delft F]]
-	[[Category: Sethi, R]]	+
-	[[Category: Wang, D]]	+
-	[[Category: ~~Sgc]]~~	+
-	~~[[Category: Wnk3~~]]	+

OCA at 06:50, 16 October 2019

OCA — Wed, 16 Oct 2019 06:50:01 GMT

←Older revision		Revision as of 06:50, 16 October 2019
Line 1:		Line 1:

	==Crystal structure of WNK3 kinase domain in a monophosphorylated apo state (A-loop swapped)==		==Crystal structure of WNK3 kinase domain in a monophosphorylated apo state (A-loop swapped)==
-	<StructureSection load='5o1v' size='340' side='right' caption='[[5o1v]], [[Resolution\|resolution]] 1.72Å' scene=''>	+	<StructureSection load='5o1v' size='340' side='right'caption='[[5o1v]], [[Resolution\|resolution]] 1.72Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5o1v]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5O1V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5O1V FirstGlance]. <br>		<table><tr><td colspan='2'>[[5o1v]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5O1V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5O1V FirstGlance]. <br>
Line 12:		Line 12:
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/WNK3_HUMAN WNK3_HUMAN]] Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively (PubMed:16275913, PubMed:16275911, PubMed:16357011). Phosphorylates WNK4. Regulates the phosphorylation of SLC12A1 and SLC12A2. Increases Ca(2+) influx mediated by TRPV5 and TRPV6 by enhancing their membrane expression level via a kinase-dependent pathway (PubMed:18768590). Inhibits the activity of KCNJ1 by decreasing its expression at the cell membrane in a non-catalytic manner.<ref>PMID:16275911</ref> <ref>PMID:16275913</ref> <ref>PMID:16357011</ref> <ref>PMID:16501604</ref> <ref>PMID:17975670</ref> <ref>PMID:18768590</ref> <ref>PMID:20525693</ref> Isoform 1, isoform 2, isoform 3 and isoform 4 stimulate the activity of SLC12A1, SLC12A2 and SLC12A3 and inhibit the activity of SLC12A4, SLC12A5, SLC12A6 and SLC12A7. According to PubMed:19470686, isoform 1 inhibits the activity of SLC12A3.<ref>PMID:19470686</ref> <ref>PMID:21613606</ref>		[[http://www.uniprot.org/uniprot/WNK3_HUMAN WNK3_HUMAN]] Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively (PubMed:16275913, PubMed:16275911, PubMed:16357011). Phosphorylates WNK4. Regulates the phosphorylation of SLC12A1 and SLC12A2. Increases Ca(2+) influx mediated by TRPV5 and TRPV6 by enhancing their membrane expression level via a kinase-dependent pathway (PubMed:18768590). Inhibits the activity of KCNJ1 by decreasing its expression at the cell membrane in a non-catalytic manner.<ref>PMID:16275911</ref> <ref>PMID:16275913</ref> <ref>PMID:16357011</ref> <ref>PMID:16501604</ref> <ref>PMID:17975670</ref> <ref>PMID:18768590</ref> <ref>PMID:20525693</ref> Isoform 1, isoform 2, isoform 3 and isoform 4 stimulate the activity of SLC12A1, SLC12A2 and SLC12A3 and inhibit the activity of SLC12A4, SLC12A5, SLC12A6 and SLC12A7. According to PubMed:19470686, isoform 1 inhibits the activity of SLC12A3.<ref>PMID:19470686</ref> <ref>PMID:21613606</ref>
		+
		+	==See Also==
		+	*[[Serine/threonine protein kinase\|Serine/threonine protein kinase]]
	== References ==		== References ==
	<references/>		<references/>
Line 17:		Line 20:
	</StructureSection>		</StructureSection>
	[[Category: Human]]		[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Non-specific serine/threonine protein kinase]]		[[Category: Non-specific serine/threonine protein kinase]]
	[[Category: Arrowsmith, C H]]		[[Category: Arrowsmith, C H]]

OCA: Protected "5o1v" [edit=sysop:move=sysop]

OCA — Mon, 06 Nov 2017 10:46:15 GMT

Protected "5o1v" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:46, 6 November 2017

OCA: New page: ==Crystal structure of WNK3 kinase domain in a monophosphorylated apo state (A-loop swapped)== == Structural highlights ==
<table><tr><td colspan='2'>[[5o1v]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5O1V OCA]. For a guided tour on the structure components use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5O1V FirstGlance]. 
</td></tr><tr id='ligand'><td class="sblockLbl">[[Ligand|Ligands:]]</td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
<tr id='NonStdRes'><td class="sblockLbl">[[Non-Standard_Residue|NonStd Res:]]</td><td class="sblockDat"><scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene></td></tr>
<tr id='gene'><td class="sblockLbl">[[Gene|Gene:]]</td><td class="sblockDat">WNK3, KIAA1566, PRKWNK3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
<tr id='activity'><td class="sblockLbl">Activity:</td><td class="sblockDat">[http://en.wikipedia.org/wiki/Non-specific_serine/threonine_protein_kinase Non-specific serine/threonine protein kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.1 2.7.11.1] </td></tr>
<tr id='resources'><td class="sblockLbl">Resources:</td><td class="sblockDat">[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5o1v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5o1v OCA], [http://pdbe.org/5o1v PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5o1v RCSB], [http://www.ebi.ac.uk/pdbsum/5o1v PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5o1v ProSAT]</td></tr>
</table>
== Function ==
[[http://www.uniprot.org/uniprot/WNK3_HUMAN WNK3_HUMAN]] Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively (PubMed:16275913, PubMed:16275911, PubMed:16357011). Phosphorylates WNK4. Regulates the phosphorylation of SLC12A1 and SLC12A2. Increases Ca(2+) influx mediated by TRPV5 and TRPV6 by enhancing their membrane expression level via a kinase-dependent pathway (PubMed:18768590). Inhibits the activity of KCNJ1 by decreasing its expression at the cell membrane in a non-catalytic manner.<ref>PMID:16275911</ref> <ref>PMID:16275913</ref> <ref>PMID:16357011</ref> <ref>PMID:16501604</ref> <ref>PMID:17975670</ref> <ref>PMID:18768590</ref> <ref>PMID:20525693</ref> Isoform 1, isoform 2, isoform 3 and isoform 4 stimulate the activity of SLC12A1, SLC12A2 and SLC12A3 and inhibit the activity of SLC12A4, SLC12A5, SLC12A6 and SLC12A7. According to PubMed:19470686, isoform 1 inhibits the activity of SLC12A3.<ref>PMID:19470686</ref> <ref>PMID:21613606</ref>
== References ==
<references/>
TOC
</StructureSection>
[[Category: Human]]
[[Category: Non-specific serine/threonine protein kinase]]
[[Category: Arrowsmith, C H]]
[[Category: Berridge, G]]
[[Category: Bountra, C]]
[[Category: Bufton, J C]]
[[Category: Bullock, A]]
[[Category: Burgess-Brown, N A]]
[[Category: Chalk, R]]
[[Category: Delft, F von]]
[[Category: Dixon-Clarke, S E]]
[[Category: Edwards, A M]]
[[Category: Fairhead, M]]
[[Category: Kopec, J]]
[[Category: Kupinska, K]]
[[Category: Pinkas, D M]]
[[Category: Structural genomic]]
[[Category: Sethi, R]]
[[Category: Wang, D]]
[[Category: Sgc]]
[[Category: Wnk3]]