5o2a - Revision history

OCA at 19:04, 29 November 2023

OCA — Wed, 29 Nov 2023 19:04:52 GMT

←Older revision		Revision as of 19:04, 29 November 2023
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	<StructureSection load='5o2a' size='340' side='right'caption='[[5o2a]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='5o2a' size='340' side='right'caption='[[5o2a]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5o2a]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5O2A OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5O2A FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5o2a]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5O2A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5O2A FirstGlance]. <br>
-	</td></tr><tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non~~-~~Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=MLY:N-DIMETHYL-LYSINE'>MLY</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.9Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5o2a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5o2a OCA], [~~http~~://pdbe.org/5o2a PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5o2a RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5o2a PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5o2a ProSAT]</span></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MLY:N-DIMETHYL-LYSINE'>MLY</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5o2a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5o2a OCA], [https://pdbe.org/5o2a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5o2a RCSB], [https://www.ebi.ac.uk/pdbsum/5o2a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5o2a ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/FOLD_ECOLI FOLD_ECOLI]] Catalyzes the oxidation of 5,10-methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10-formyltetrahydrofolate. This enzyme is specific for NADP.<ref>PMID:1748668</ref>	+	[https://www.uniprot.org/uniprot/FOLD_ECOLI FOLD_ECOLI] Catalyzes the oxidation of 5,10-methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10-formyltetrahydrofolate. This enzyme is specific for NADP.<ref>PMID:1748668</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Escherichia coli K-12]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Koehnke, J]]	+	[[Category: Koehnke J]]
-	[[Category: Sikandar, A]]	+	[[Category: Sikandar A]]
-	~~[[Category: Carolacton]]~~	+
-	~~[[Category: Fold]]~~	+
-	~~[[Category: Inhibitor]]~~	+
-	~~[[Category: Natural product]]~~	+
-	~~[[Category: Oxidoreductase~~]]	+

OCA at 12:16, 26 August 2020

OCA — Wed, 26 Aug 2020 12:16:40 GMT

←Older revision		Revision as of 12:16, 26 August 2020
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	==FolD Q98H==		==FolD Q98H==
-	<StructureSection load='5o2a' size='340' side='right' caption='[[5o2a]], [[Resolution\|resolution]] 1.90Å' scene=''>	+	<StructureSection load='5o2a' size='340' side='right'caption='[[5o2a]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5o2a]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5O2A OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5O2A FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5o2a]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5O2A OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5O2A FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MLY:N-DIMETHYL-LYSINE'>MLY</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MLY:N-DIMETHYL-LYSINE'>MLY</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5o2a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5o2a OCA], [http://pdbe.org/5o2a PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5o2a RCSB], [http://www.ebi.ac.uk/pdbsum/5o2a PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5o2a ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5o2a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5o2a OCA], [http://pdbe.org/5o2a PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5o2a RCSB], [http://www.ebi.ac.uk/pdbsum/5o2a PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5o2a ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Koehnke, J]]		[[Category: Koehnke, J]]
	[[Category: Sikandar, A]]		[[Category: Sikandar, A]]

OCA at 06:19, 28 February 2018

OCA — Wed, 28 Feb 2018 06:19:34 GMT

←Older revision		Revision as of 06:19, 28 February 2018
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-	'''Unreleased structure'''

-	~~The entry~~ 5o2a is ~~ON HOLD~~ ~~until Paper~~ Publication	+	==FolD Q98H==
		+	<StructureSection load='5o2a' size='340' side='right' caption='[[5o2a]], [[Resolution\|resolution]] 1.90Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5o2a]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5O2A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5O2A FirstGlance]. <br>
		+	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MLY:N-DIMETHYL-LYSINE'>MLY</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5o2a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5o2a OCA], [http://pdbe.org/5o2a PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5o2a RCSB], [http://www.ebi.ac.uk/pdbsum/5o2a PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5o2a ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/FOLD_ECOLI FOLD_ECOLI]] Catalyzes the oxidation of 5,10-methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10-formyltetrahydrofolate. This enzyme is specific for NADP.<ref>PMID:1748668</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The natural product carolacton is a macrolide keto-carboxylic acid produced by the myxobacterium Sorangium cellulosum, and was originally described as an antibacterial compound. Here we show that carolacton targets FolD, a key enzyme from the folate-dependent C1 metabolism. We characterize the interaction between bacterial FolD and carolacton biophysically, structurally and biochemically. Carolacton binds FolD with nanomolar affinity, and the crystal structure of the FolD-carolacton complex reveals the mode of binding. We show that the human FolD orthologs, MTHFD1 and MTHFD2, are also inhibited in the low nM range, and that micromolar concentrations of carolacton inhibit the growth of cancer cell lines. As mitochondrial MTHFD2 is known to be upregulated in cancer cells, it may be possible to use carolacton as an inhibitor tool compound to assess MTHFD2 as an anti-cancer target.

-	~~Authors: Koehnke, J~~., ~~Sikandar~~, A.	+	The natural product carolacton inhibits folate-dependent C1 metabolism by targeting FolD/MTHFD.,Fu C, Sikandar A, Donner J, Zaburannyi N, Herrmann J, Reck M, Wagner-Dobler I, Koehnke J, Muller R Nat Commun. 2017 Nov 16;8(1):1529. doi: 10.1038/s41467-017-01671-5. PMID:29142318<ref>PMID:29142318</ref>

-	~~Description: FolD Q98H~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category: Unreleased Structures]]~~	+	</div>
-	~~[[Category~~: ~~Sikandar, A]]~~	+	<div class="pdbe-citations 5o2a" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Koehnke, J]]		[[Category: Koehnke, J]]
		+	[[Category: Sikandar, A]]
		+	[[Category: Carolacton]]
		+	[[Category: Fold]]
		+	[[Category: Inhibitor]]
		+	[[Category: Natural product]]
		+	[[Category: Oxidoreductase]]

OCA: Protected "5o2a" [edit=sysop:move=sysop]

OCA — Wed, 27 Sep 2017 11:58:50 GMT

Protected "5o2a" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:58, 27 September 2017

OCA: New page: '''Unreleased structure''' The entry 5o2a is ON HOLD until Paper Publication Authors: Koehnke, J., Sikandar, A. Description: FolD Q98H Category: Unreleased Structures [[Category: S...

OCA — Wed, 27 Sep 2017 11:58:49 GMT

New page: '''Unreleased structure''' The entry 5o2a is ON HOLD until Paper Publication Authors: Koehnke, J., Sikandar, A. Description: FolD Q98H Category: Unreleased Structures [[Category: S...

New page

'''Unreleased structure'''

The entry 5o2a is ON HOLD until Paper Publication

Authors: Koehnke, J., Sikandar, A.

Description: FolD Q98H
[[Category: Unreleased Structures]]
[[Category: Sikandar, A]]
[[Category: Koehnke, J]]