5o3j - Revision history

OCA at 19:06, 29 November 2023

OCA — Wed, 29 Nov 2023 19:06:32 GMT

←Older revision		Revision as of 19:06, 29 November 2023
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	<StructureSection load='5o3j' size='340' side='right'caption='[[5o3j]], [[Resolution\|resolution]] 2.97Å' scene=''>		<StructureSection load='5o3j' size='340' side='right'caption='[[5o3j]], [[Resolution\|resolution]] 2.97Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5o3j]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5O3J OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5O3J FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5o3j]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5O3J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5O3J FirstGlance]. <br>
-	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~TIA1 (~~[~~http://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=9606 HUMAN])~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.97Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5o3j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5o3j OCA], [~~http~~://pdbe.org/5o3j PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5o3j RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5o3j PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5o3j ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5o3j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5o3j OCA], [https://pdbe.org/5o3j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5o3j RCSB], [https://www.ebi.ac.uk/pdbsum/5o3j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5o3j ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/TIA1_HUMAN TIA1_HUMAN]] Involved in alternative pre-RNA splicing and regulation of mRNA translation by binding to AU-rich elements (AREs) located in mRNA 3' untranslated regions (3' UTRs). Possesses nucleolytic activity against cytotoxic lymphocyte target cells. May be involved in apoptosis.	+	[https://www.uniprot.org/uniprot/TIA1_HUMAN TIA1_HUMAN] Involved in alternative pre-RNA splicing and regulation of mRNA translation by binding to AU-rich elements (AREs) located in mRNA 3' untranslated regions (3' UTRs). Possesses nucleolytic activity against cytotoxic lymphocyte target cells. May be involved in apoptosis.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Hennig, J]]	+	[[Category: Hennig J]]
-	[[Category: Jagtap~~, P K.A~~]]	+	[[Category: Jagtap PKA]]
-	[[Category: Sattler, M]]	+	[[Category: Sattler M]]
-	[[Category: Sonntag, M]]	+	[[Category: Sonntag M]]
-	~~[[Category: Rna binding protein]]~~	+
-	~~[[Category: Rrm]]~~	+
-	~~[[Category: Tia-1~~]]	+

OCA at 06:51, 16 October 2019

OCA — Wed, 16 Oct 2019 06:51:04 GMT

←Older revision		Revision as of 06:51, 16 October 2019
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	==Crystal structure of TIA-1 RRM2 in complex with RNA==		==Crystal structure of TIA-1 RRM2 in complex with RNA==
-	<StructureSection load='5o3j' size='340' side='right' caption='[[5o3j]], [[Resolution\|resolution]] 2.97Å' scene=''>	+	<StructureSection load='5o3j' size='340' side='right'caption='[[5o3j]], [[Resolution\|resolution]] 2.97Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5o3j]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5O3J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5O3J FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5o3j]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5O3J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5O3J FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5o3j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5o3j OCA], [http://pdbe.org/5o3j PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5o3j RCSB], [http://www.ebi.ac.uk/pdbsum/5o3j PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5o3j ProSAT]</span></td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TIA1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5o3j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5o3j OCA], [http://pdbe.org/5o3j PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5o3j RCSB], [http://www.ebi.ac.uk/pdbsum/5o3j PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5o3j ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Hennig, J]]		[[Category: Hennig, J]]
	[[Category: Jagtap, P K.A]]		[[Category: Jagtap, P K.A]]

OCA: Protected "5o3j" [edit=sysop:move=sysop]

OCA — Wed, 09 Aug 2017 09:00:45 GMT

Protected "5o3j" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:00, 9 August 2017

OCA: New page: ==Crystal structure of TIA-1 RRM2 in complex with RNA== == Struct...

OCA — Wed, 09 Aug 2017 09:00:39 GMT

New page: ==Crystal structure of TIA-1 RRM2 in complex with RNA== <StructureSection load='5o3j' size='340' side='right' caption='5o3j, resolution 2.97Å' scene=''> == Struct...

New page

==Crystal structure of TIA-1 RRM2 in complex with RNA==
<StructureSection load='5o3j' size='340' side='right' caption='[[5o3j]], [[Resolution|resolution]] 2.97Å' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5o3j]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5O3J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5O3J FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5o3j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5o3j OCA], [http://pdbe.org/5o3j PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5o3j RCSB], [http://www.ebi.ac.uk/pdbsum/5o3j PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5o3j ProSAT]</span></td></tr>
</table>
== Function ==
[[http://www.uniprot.org/uniprot/TIA1_HUMAN TIA1_HUMAN]] Involved in alternative pre-RNA splicing and regulation of mRNA translation by binding to AU-rich elements (AREs) located in mRNA 3' untranslated regions (3' UTRs). Possesses nucleolytic activity against cytotoxic lymphocyte target cells. May be involved in apoptosis.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Multi-domain proteins play critical roles in fine-tuning essential processes in cellular signaling and gene regulation. Typically, multiple globular domains that are connected by flexible linkers undergo dynamic rearrangements upon binding to protein, DNA or RNA ligands. RNA binding proteins (RBPs) represent an important class of multi-domain proteins, which regulate gene expression by recognizing linear or structured RNA sequence motifs. Here, we employ segmental perdeuteration of the three RNA recognition motif (RRM) domains in the RBP TIA-1 using Sortase A mediated protein ligation. We show that domain-selective perdeuteration combined with contrast-matched small-angle neutron scattering (SANS), SAXS and computational modeling provides valuable information to precisely define relative domain arrangements. The approach is generally applicable to study conformational arrangements of individual domains in multi-domain proteins and changes induced by ligand binding.

Segmental, Domain-Selective Perdeuteration and Small-Angle Neutron Scattering for Structural Analysis of Multi-Domain Proteins.,Sonntag M, Jagtap PKA, Simon B, Appavou MS, Geerlof A, Stehle R, Gabel F, Hennig J, Sattler M Angew Chem Int Ed Engl. 2017 Aug 1;56(32):9322-9325. doi: 10.1002/anie.201702904., Epub 2017 Jul 5. PMID:28636238<ref>PMID:28636238</ref>

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5o3j" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Hennig, J]]
[[Category: Jagtap, P K.A]]
[[Category: Sattler, M]]
[[Category: Sonntag, M]]
[[Category: Rna binding protein]]
[[Category: Rrm]]
[[Category: Tia-1]]