5o4q - Revision history

OCA at 19:08, 29 November 2023

OCA — Wed, 29 Nov 2023 19:08:10 GMT

←Older revision		Revision as of 19:08, 29 November 2023
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	<StructureSection load='5o4q' size='340' side='right'caption='[[5o4q]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='5o4q' size='340' side='right'caption='[[5o4q]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5o4q]] is a 12 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"actinomyces_griseoflavus"_krainsky_1914 "actinomyces~~ griseoflavus~~" krainsky 1914~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5O4Q OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5O4Q FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5o4q]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_griseoflavus Streptomyces griseoflavus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5O4Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5O4Q FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AZI:AZIDE+ION'>AZI</scene>, <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.9Å</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Laccase Laccase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.10.3.2 1.10.3.2] </span></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AZI:AZIDE+ION'>AZI</scene>, <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5o4q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5o4q OCA], [~~http~~://pdbe.org/5o4q PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5o4q RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5o4q PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5o4q ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5o4q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5o4q OCA], [https://pdbe.org/5o4q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5o4q RCSB], [https://www.ebi.ac.uk/pdbsum/5o4q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5o4q ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A0M4FJ81_9ACTN A0A0M4FJ81_9ACTN]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Actinomyces griseoflavus krainsky 1914]]
-	[[Category: Laccase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Gabdulkhakov, A G]]
-	[[Category: Tishchenko, T V]]
-	[[Category: Oxidoreductase]]
	[[Category: Streptomyces griseoflavus]]		[[Category: Streptomyces griseoflavus]]
-	[[Category: ~~Two-domain laccase~~]]	+	[[Category: Gabdulkhakov AG]]
		+	[[Category: Tishchenko TV]]

OCA at 06:52, 16 October 2019

OCA — Wed, 16 Oct 2019 06:52:00 GMT

←Older revision		Revision as of 06:52, 16 October 2019
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	==Crystal Structure of mutant M54L/M64L/M96L of Two-Domain Laccase from Streptomyces griseoflavus with 0.25 mM copper sulfate on growth medium==		==Crystal Structure of mutant M54L/M64L/M96L of Two-Domain Laccase from Streptomyces griseoflavus with 0.25 mM copper sulfate on growth medium==
-	<StructureSection load='5o4q' size='340' side='right' caption='[[5o4q]], [[Resolution\|resolution]] 1.90Å' scene=''>	+	<StructureSection load='5o4q' size='340' side='right'caption='[[5o4q]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5o4q]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/"actinomyces_griseoflavus"_krainsky_1914 "actinomyces griseoflavus" krainsky 1914]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5O4Q OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5O4Q FirstGlance]. <br>		<table><tr><td colspan='2'>[[5o4q]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/"actinomyces_griseoflavus"_krainsky_1914 "actinomyces griseoflavus" krainsky 1914]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5O4Q OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5O4Q FirstGlance]. <br>
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	</div>		</div>
	<div class="pdbe-citations 5o4q" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5o4q" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Laccase 3D structures\|Laccase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
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	[[Category: Actinomyces griseoflavus krainsky 1914]]		[[Category: Actinomyces griseoflavus krainsky 1914]]
	[[Category: Laccase]]		[[Category: Laccase]]
		+	[[Category: Large Structures]]
	[[Category: Gabdulkhakov, A G]]		[[Category: Gabdulkhakov, A G]]
	[[Category: Tishchenko, T V]]		[[Category: Tishchenko, T V]]

OCA at 05:51, 25 April 2018

OCA — Wed, 25 Apr 2018 05:51:07 GMT

←Older revision		Revision as of 05:51, 25 April 2018
Line 3:		Line 3:
	<StructureSection load='5o4q' size='340' side='right' caption='[[5o4q]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='5o4q' size='340' side='right' caption='[[5o4q]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5o4q]] is a 12 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5O4Q OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5O4Q FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5o4q]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/"actinomyces_griseoflavus"_krainsky_1914 "actinomyces griseoflavus" krainsky 1914]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5O4Q OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5O4Q FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AZI:AZIDE+ION'>AZI</scene>, <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AZI:AZIDE+ION'>AZI</scene>, <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Laccase Laccase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.10.3.2 1.10.3.2] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Laccase Laccase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.10.3.2 1.10.3.2] </span></td></tr>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Actinomyces griseoflavus krainsky 1914]]
	[[Category: Laccase]]		[[Category: Laccase]]
	[[Category: Gabdulkhakov, A G]]		[[Category: Gabdulkhakov, A G]]

OCA at 06:30, 28 March 2018

OCA — Wed, 28 Mar 2018 06:30:04 GMT

←Older revision		Revision as of 06:30, 28 March 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5o4q is ~~ON HOLD~~	+	==Crystal Structure of mutant M54L/M64L/M96L of Two-Domain Laccase from Streptomyces griseoflavus with 0.25 mM copper sulfate on growth medium==
		+	<StructureSection load='5o4q' size='340' side='right' caption='[[5o4q]], [[Resolution\|resolution]] 1.90Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5o4q]] is a 12 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5O4Q OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5O4Q FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AZI:AZIDE+ION'>AZI</scene>, <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Laccase Laccase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.10.3.2 1.10.3.2] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5o4q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5o4q OCA], [http://pdbe.org/5o4q PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5o4q RCSB], [http://www.ebi.ac.uk/pdbsum/5o4q PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5o4q ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Laccase belongs to the family of copper-containing oxidases. A study was made of the mechanism that sustains the incorporation of copper ions into the T2/T3 centers of recombinant two-domain laccase Streptomyces griseoflavus Ac-993. The occupancy of the T3 center by copper ions was found to increase with an increasing copper content in the culture medium and after dialysis of the protein preparation against a copper sulfate-containing buffer. The T2 center was filled only when overproducer strain cells were grown at a higher copper concentration in the medium. Two-domain laccases were assumed to possess a channel that serves to deliver copper ions to the T3 center during the formation of the three-dimensional laccase conformation and dialysis of the protein preparation. A narrower channel leads to the T2 center in two-domain laccases compared with three-domain ones, rendering the center less accessible for copper atoms. The incorporation of copper ions into the T2 center of two-domain laccases is likely to occur in the course of their biosynthesis or the formation of a functional trimer.

-	~~Authors:~~ Gabdulkhakov, ~~A.G.~~, ~~Tishchenko~~, T.V.	+	[Incorporation of Copper Ions into T2/T3 Centers of Two-Domain Laccases].,Gabdulkhakov AG, Kostareva OS, Kolyadenko IA, Mikhaylina AO, Trubitsina LI, Tishchenko SV Mol Biol (Mosk). 2018 Jan-Feb;52(1):29-35. doi: 10.7868/S0026898418010056. PMID:29512633<ref>PMID:29512633</ref>

-	~~Description: Crystal Structure of mutant M54L~~/~~M64L/M96L~~ of ~~Two~~-~~Domain~~ Laccase ~~from Streptomyces griseoflavus with 0.25 mM copper sulfate on growth medium~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
-	[[Category: Tishchenko, T.V]]	+	<div class="pdbe-citations 5o4q" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Gabdulkhakov, A.G~~]]	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Laccase]]
		+	[[Category: Gabdulkhakov, A G]]
		+	[[Category: Tishchenko, T V]]
		+	[[Category: Oxidoreductase]]
		+	[[Category: Streptomyces griseoflavus]]
		+	[[Category: Two-domain laccase]]

OCA: Protected "5o4q" [edit=sysop:move=sysop]

OCA — Wed, 27 Sep 2017 12:02:29 GMT

Protected "5o4q" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:02, 27 September 2017

OCA: New page: '''Unreleased structure''' The entry 5o4q is ON HOLD Authors: Gabdulkhakov, A.G., Tishchenko, T.V. Description: Crystal Structure of mutant M54L/M64L/M96L of Two-Domain Laccase from St...

OCA — Wed, 27 Sep 2017 12:02:28 GMT

New page: '''Unreleased structure''' The entry 5o4q is ON HOLD Authors: Gabdulkhakov, A.G., Tishchenko, T.V. Description: Crystal Structure of mutant M54L/M64L/M96L of Two-Domain Laccase from St...

New page

'''Unreleased structure'''

The entry 5o4q is ON HOLD

Authors: Gabdulkhakov, A.G., Tishchenko, T.V.

Description: Crystal Structure of mutant M54L/M64L/M96L of Two-Domain Laccase from Streptomyces griseoflavus with 0.25 mM copper sulfate on growth medium
[[Category: Unreleased Structures]]
[[Category: Tishchenko, T.V]]
[[Category: Gabdulkhakov, A.G]]