5o59 - Revision history

OCA at 19:08, 29 November 2023

OCA — Wed, 29 Nov 2023 19:08:36 GMT

←Older revision		Revision as of 19:08, 29 November 2023
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	<StructureSection load='5o59' size='340' side='right'caption='[[5o59]], [[Resolution\|resolution]] 1.75Å' scene=''>		<StructureSection load='5o59' size='340' side='right'caption='[[5o59]], [[Resolution\|resolution]] 1.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5o59]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Hypocrea_atroviridis Hypocrea atroviridis~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5O59 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5O59 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5o59]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Trichoderma_atroviride_IMI_206040 Trichoderma atroviride IMI 206040]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5O59 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5O59 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BTB:2-[BIS-(2-HYDROXY-ETHYL)-AMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>BTB</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=GS1:1-THIO-BETA-D-GLUCOPYRANOSE'>GS1</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=~~PEG~~:~~DI(HYDROXYETHYL)ETHER~~'>~~PEG~~</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.75Å</td></tr>
-	~~<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat">~~<scene name='pdbligand=~~PCA~~:~~PYROGLUTAMIC+ACID~~'>~~PCA~~</scene></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BTB:2-[BIS-(2-HYDROXY-ETHYL)-AMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>BTB</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=GS1:1-THIO-BETA-D-GLUCOPYRANOSE'>GS1</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5o59 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5o59 OCA], [~~http~~://pdbe.org/5o59 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5o59 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5o59 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5o59 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5o59 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5o59 OCA], [https://pdbe.org/5o59 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5o59 RCSB], [https://www.ebi.ac.uk/pdbsum/5o59 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5o59 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/G9NTY1_HYPAI G9NTY1_HYPAI]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Hypocrea atroviridis]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Borisova, A S~~]]	+	[[Category: Trichoderma atroviride IMI 206040]]
-	[[Category: ~~Hansson, H~~]]	+	[[Category: Borisova AS]]
-	[[Category: ~~Stahlberg, J~~]]	+	[[Category: Hansson H]]
-	[[Category: ~~Cellobiohydrolase]]~~	+	[[Category: Stahlberg J]]
-	~~[[Category: Cellulase]]~~	+
-	~~[[Category: Glycoside hydrolase]]~~	+
-	~~[[Category: Hydrolase~~]]	+

OCA at 07:47, 11 March 2020

OCA — Wed, 11 Mar 2020 07:47:55 GMT

←Older revision		Revision as of 07:47, 11 March 2020
Line 1:		Line 1:

	==Cellobiohydrolase Cel7A from T. atroviride==		==Cellobiohydrolase Cel7A from T. atroviride==
-	<StructureSection load='5o59' size='340' side='right' caption='[[5o59]], [[Resolution\|resolution]] 1.75Å' scene=''>	+	<StructureSection load='5o59' size='340' side='right'caption='[[5o59]], [[Resolution\|resolution]] 1.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5o59]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Hypocrea_atroviridis Hypocrea atroviridis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5O59 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5O59 FirstGlance]. <br>		<table><tr><td colspan='2'>[[5o59]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Hypocrea_atroviridis Hypocrea atroviridis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5O59 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5O59 FirstGlance]. <br>
Line 17:		Line 17:
	</div>		</div>
	<div class="pdbe-citations 5o59" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5o59" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Cellobiohydrolase 3D structures\|Cellobiohydrolase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
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	</StructureSection>		</StructureSection>
	[[Category: Hypocrea atroviridis]]		[[Category: Hypocrea atroviridis]]
		+	[[Category: Large Structures]]
	[[Category: Borisova, A S]]		[[Category: Borisova, A S]]
	[[Category: Hansson, H]]		[[Category: Hansson, H]]

OCA at 05:49, 31 January 2018

OCA — Wed, 31 Jan 2018 05:49:59 GMT

←Older revision		Revision as of 05:49, 31 January 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5o59 is ~~ON HOLD until Paper~~ Publication	+	==Cellobiohydrolase Cel7A from T. atroviride==
		+	<StructureSection load='5o59' size='340' side='right' caption='[[5o59]], [[Resolution\|resolution]] 1.75Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5o59]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Hypocrea_atroviridis Hypocrea atroviridis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5O59 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5O59 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BTB:2-[BIS-(2-HYDROXY-ETHYL)-AMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>BTB</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=GS1:1-THIO-BETA-D-GLUCOPYRANOSE'>GS1</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5o59 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5o59 OCA], [http://pdbe.org/5o59 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5o59 RCSB], [http://www.ebi.ac.uk/pdbsum/5o59 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5o59 ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Background: The ascomycete fungus Trichoderma reesei is the predominant source of enzymes for industrial conversion of lignocellulose. Its glycoside hydrolase family 7 cellobiohydrolase (GH7 CBH) TreCel7A constitutes nearly half of the enzyme cocktail by weight and is the major workhorse in the cellulose hydrolysis process. The orthologs from Trichoderma atroviride (TatCel7A) and Trichoderma harzianum (ThaCel7A) show high sequence identity with TreCel7A, ~ 80%, and represent naturally evolved combinations of cellulose-binding tunnel-enclosing loop motifs, which have been suggested to influence intrinsic cellobiohydrolase properties, such as endo-initiation, processivity, and off-rate. Results: The TatCel7A, ThaCel7A, and TreCel7A enzymes were characterized for comparison of function. The catalytic domain of TatCel7A was crystallized, and two structures were determined: without ligand and with thio-cellotriose in the active site. Initial hydrolysis of bacterial cellulose was faster with TatCel7A than either ThaCel7A or TreCel7A. In synergistic saccharification of pretreated corn stover, both TatCel7A and ThaCel7A were more efficient than TreCel7A, although TatCel7A was more sensitive to thermal inactivation. Structural analyses and molecular dynamics (MD) simulations were performed to elucidate important structure/function correlations. Moreover, reverse conservation analysis (RCA) of sequence diversity revealed divergent regions of interest located outside the cellulose-binding tunnel of Trichoderma spp. GH7 CBHs. Conclusions: We hypothesize that the combination of loop motifs is the main determinant for the observed differences in Cel7A activity on cellulosic substrates. Fine-tuning of the loop flexibility appears to be an important evolutionary target in Trichoderma spp., a conclusion supported by the RCA data. Our results indicate that, for industrial use, it would be beneficial to combine loop motifs from TatCel7A with the thermostability features of TreCel7A. Furthermore, one region implicated in thermal unfolding is suggested as a primary target for protein engineering.

-	~~Authors: Borisova~~, A.S., ~~Stahlberg~~, J., ~~Hansson~~, H.	+	Correlation of structure, function and protein dynamics in GH7 cellobiohydrolases from Trichoderma atroviride, T. reesei and T. harzianum.,Borisova AS, Eneyskaya EV, Jana S, Badino SF, Kari J, Amore A, Karlsson M, Hansson H, Sandgren M, Himmel ME, Westh P, Payne CM, Kulminskaya AA, Stahlberg J Biotechnol Biofuels. 2018 Jan 13;11:5. doi: 10.1186/s13068-017-1006-7., eCollection 2018. PMID:29344086<ref>PMID:29344086</ref>

-	~~Description: Cellobiohydrolase Cel7A from T~~. ~~atroviride~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 5o59" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Hypocrea atroviridis]]
		+	[[Category: Borisova, A S]]
	[[Category: Hansson, H]]		[[Category: Hansson, H]]
	[[Category: Stahlberg, J]]		[[Category: Stahlberg, J]]
-	[[Category: ~~Borisova, A.S~~]]	+	[[Category: Cellobiohydrolase]]
		+	[[Category: Cellulase]]
		+	[[Category: Glycoside hydrolase]]
		+	[[Category: Hydrolase]]

OCA: Protected "5o59" [edit=sysop:move=sysop]

OCA — Wed, 27 Sep 2017 12:03:10 GMT

Protected "5o59" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:03, 27 September 2017

OCA: New page: '''Unreleased structure''' The entry 5o59 is ON HOLD until Paper Publication Authors: Borisova, A.S., Stahlberg, J., Hansson, H. Description: Cellobiohydrolase Cel7A from T. atroviride...

OCA — Wed, 27 Sep 2017 12:03:09 GMT

New page: '''Unreleased structure''' The entry 5o59 is ON HOLD until Paper Publication Authors: Borisova, A.S., Stahlberg, J., Hansson, H. Description: Cellobiohydrolase Cel7A from T. atroviride...

New page

'''Unreleased structure'''

The entry 5o59 is ON HOLD until Paper Publication

Authors: Borisova, A.S., Stahlberg, J., Hansson, H.

Description: Cellobiohydrolase Cel7A from T. atroviride
[[Category: Unreleased Structures]]
[[Category: Hansson, H]]
[[Category: Stahlberg, J]]
[[Category: Borisova, A.S]]