5o6u - Revision history

OCA at 19:09, 29 November 2023

OCA — Wed, 29 Nov 2023 19:09:59 GMT

←Older revision		Revision as of 19:09, 29 November 2023
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	==Structure of the Cascade-I-Fv R-loop complex from Shewanella putrefaciens==		==Structure of the Cascade-I-Fv R-loop complex from Shewanella putrefaciens==
-	<StructureSection load='5o6u' size='340' side='right' caption='[[5o6u]], [[Resolution\|resolution]] 3.25Å' scene=''>	+	<StructureSection load='5o6u' size='340' side='right'caption='[[5o6u]], [[Resolution\|resolution]] 3.25Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5o6u]] is a 8 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/ ~~] and [http://en.wikipedia.org/wiki/Shepc Shepc~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5O6U OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5O6U FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5o6u]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Shewanella_putrefaciens_CN-32 Shewanella putrefaciens CN-32]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5O6U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5O6U FirstGlance]. <br>
-	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>Sputcn32_1823 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~319224 SHEPC])~~, ~~Sputcn32_1821 (~~[~~http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=319224 SHEPC~~]~~), Sputcn32_1822 ([http://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=319224 SHEPC])~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.25Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5o6u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5o6u OCA], [~~http~~://pdbe.org/5o6u PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5o6u RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5o6u PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5o6u ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5o6u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5o6u OCA], [https://pdbe.org/5o6u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5o6u RCSB], [https://www.ebi.ac.uk/pdbsum/5o6u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5o6u ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A4Y6G3_SHEPC A4Y6G3_SHEPC]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Shepc~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Altegoer, F~~]]	+	[[Category: Shewanella putrefaciens CN-32]]
-	[[Category: ~~Bange, G~~]]	+	[[Category: Altegoer F]]
-	[[Category: ~~Pausch, P~~]]	+	[[Category: Bange G]]
-	[[Category: ~~Adaptive immunity]]~~	+	[[Category: Pausch P]]
-	~~[[Category: Antiviral protein]]~~	+
-	~~[[Category: Cascade]]~~	+
-	~~[[Category: Crispr]]~~	+
-	~~[[Category: Rna surveillance~~]]	+

OCA at 07:30, 21 February 2019

OCA — Thu, 21 Feb 2019 07:30:09 GMT

←Older revision		Revision as of 07:30, 21 February 2019
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	</div>		</div>
	<div class="pdbe-citations 5o6u" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5o6u" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[CRISPR subtype I-F\|CRISPR subtype I-F]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 00:02, 16 November 2017

OCA — Thu, 16 Nov 2017 00:02:49 GMT

←Older revision		Revision as of 00:02, 16 November 2017
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	<StructureSection load='5o6u' size='340' side='right' caption='[[5o6u]], [[Resolution\|resolution]] 3.25Å' scene=''>		<StructureSection load='5o6u' size='340' side='right' caption='[[5o6u]], [[Resolution\|resolution]] 3.25Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5o6u]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5O6U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5O6U FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5o6u]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/ ] and [http://en.wikipedia.org/wiki/Shepc Shepc]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5O6U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5O6U FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5o6u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5o6u OCA], [http://pdbe.org/5o6u PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5o6u RCSB], [http://www.ebi.ac.uk/pdbsum/5o6u PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5o6u ProSAT]</span></td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Sputcn32_1823 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=319224 SHEPC]), Sputcn32_1821 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=319224 SHEPC]), Sputcn32_1822 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=319224 SHEPC])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5o6u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5o6u OCA], [http://pdbe.org/5o6u PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5o6u RCSB], [http://www.ebi.ac.uk/pdbsum/5o6u PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5o6u ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Shepc]]
	[[Category: Altegoer, F]]		[[Category: Altegoer, F]]
	[[Category: Bange, G]]		[[Category: Bange, G]]

OCA: Protected "5o6u" [edit=sysop:move=sysop]

OCA — Thu, 17 Aug 2017 06:06:45 GMT

Protected "5o6u" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:06, 17 August 2017

OCA: New page: ==Structure of the Cascade-I-Fv R-loop complex from Shewanella putrefaciens== == Structural highlights ==
<table><tr><td colspan='2'>[[5o6u]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5O6U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5O6U FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5o6u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5o6u OCA], [http://pdbe.org/5o6u PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5o6u RCSB], [http://www.ebi.ac.uk/pdbsum/5o6u PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5o6u ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
CRISPR-Cas systems are prokaryotic immune systems against invading nucleic acids. Type I CRISPR-Cas systems employ highly diverse, multi-subunit surveillance Cascade complexes that facilitate duplex formation between crRNA and complementary target DNA for R-loop formation, retention, and DNA degradation by the subsequently recruited nuclease Cas3. Typically, the large subunit recognizes bona fide targets through the PAM (protospacer adjacent motif), and the small subunit guides the non-target DNA strand. Here, we present the Apo- and target-DNA-bound structures of the I-Fv (type I-F variant) Cascade lacking the small and large subunits. Large and small subunits are functionally replaced by the 5' terminal crRNA cap Cas5fv and the backbone protein Cas7fv, respectively. Cas5fv facilitates PAM recognition from the DNA major groove site, in contrast to all other described type I systems. Comparison of the type I-Fv Cascade with an anti-CRISPR protein-bound I-F Cascade reveals that the type I-Fv structure differs substantially at known anti-CRISPR protein target sites and might therefore be resistant to viral Cascade interception.

Structural Variation of Type I-F CRISPR RNA Guided DNA Surveillance.,Pausch P, Muller-Esparza H, Gleditzsch D, Altegoer F, Randau L, Bange G Mol Cell. 2017 Aug 2. pii: S1097-2765(17)30494-X. doi:, 10.1016/j.molcel.2017.06.036. PMID:28781236<ref>PMID:28781236</ref>

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5o6u" style="background-color:#fffaf0;"></div>
== References ==
<references/>
TOC
</StructureSection>
[[Category: Altegoer, F]]
[[Category: Bange, G]]
[[Category: Pausch, P]]
[[Category: Adaptive immunity]]
[[Category: Antiviral protein]]
[[Category: Cascade]]
[[Category: Crispr]]
[[Category: Rna surveillance]]