5o8t - Revision history

OCA at 09:37, 6 December 2023

OCA — Wed, 06 Dec 2023 09:37:11 GMT

←Older revision		Revision as of 09:37, 6 December 2023
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	<StructureSection load='5o8t' size='340' side='right'caption='[[5o8t]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='5o8t' size='340' side='right'caption='[[5o8t]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5o8t]] is a 5 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Aplca Aplca~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5O8T OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5O8T FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5o8t]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Aplysia_californica Aplysia californica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5O8T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5O8T FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=SY9:STRYCHNINE'>SY9</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.2Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5o8t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5o8t OCA], [~~http~~://pdbe.org/5o8t PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5o8t RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5o8t PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5o8t ProSAT]</span></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=SY9:STRYCHNINE'>SY9</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5o8t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5o8t OCA], [https://pdbe.org/5o8t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5o8t RCSB], [https://www.ebi.ac.uk/pdbsum/5o8t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5o8t ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q8WSF8_APLCA Q8WSF8_APLCA]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 5o8t" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5o8t" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Acetylcholine binding protein 3D structures\|Acetylcholine binding protein 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Aplca~~]]	+	[[Category: Aplysia californica]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Dawson, A]]	+	[[Category: Dawson A]]
-	[[Category: Hunter~~, W N]]~~	+	[[Category: Hunter WN]]
-	~~[[Category: Souza, J O.de~~]]	+	[[Category: Trumper P]]
-	[[Category: Trumper, P]]	+	[[Category: De Souza JO]]
-	[[Category: ~~Receptor]]~~	+
-	~~[[Category: Signaling protein~~]]	+

OCA at 04:34, 13 February 2020

OCA — Thu, 13 Feb 2020 04:34:18 GMT

←Older revision		Revision as of 04:34, 13 February 2020
Line 1:		Line 1:

	==Crystal structure of wild type Aplysia californica AChBP in complex with strychnine==		==Crystal structure of wild type Aplysia californica AChBP in complex with strychnine==
-	<StructureSection load='5o8t' size='340' side='right' caption='[[5o8t]], [[Resolution\|resolution]] 2.20Å' scene=''>	+	<StructureSection load='5o8t' size='340' side='right'caption='[[5o8t]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5o8t]] is a 5 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5O8T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5O8T FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5o8t]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Aplca Aplca]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5O8T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5O8T FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=SY9:STRYCHNINE'>SY9</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=SY9:STRYCHNINE'>SY9</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5o8t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5o8t OCA], [http://pdbe.org/5o8t PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5o8t RCSB], [http://www.ebi.ac.uk/pdbsum/5o8t PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5o8t ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5o8t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5o8t OCA], [http://pdbe.org/5o8t PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5o8t RCSB], [http://www.ebi.ac.uk/pdbsum/5o8t PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5o8t ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Protein-engineering methods have been exploited to produce a surrogate system for the extracellular neurotransmitter-binding site of a heteromeric human ligand-gated ion channel, the glycine receptor. This approach circumvents two major issues: the inherent experimental difficulties in working with a membrane-bound ion channel and the complication that a heteromeric assembly is necessary to create a key, physiologically relevant binding site. Residues that form the orthosteric site in a highly stable ortholog, acetylcholine-binding protein, were selected for substitution. Recombinant proteins were prepared and characterized in stepwise fashion exploiting a range of biophysical techniques, including X-ray crystallography, married to the use of selected chemical probes. The decision making and development of the surrogate, which is termed a glycine-binding protein, are described, and comparisons are provided with wild-type and homomeric systems that establish features of molecular recognition in the binding site and the confidence that the system is suited for use in early-stage drug discovery targeting a heteromeric alpha/beta glycine receptor.
		+
		+	Engineering a surrogate human heteromeric alpha/beta glycine receptor orthosteric site exploiting the structural homology and stability of acetylcholine-binding protein.,Dawson A, Trumper P, de Souza JO, Parker H, Jones MJ, Hales TG, Hunter WN IUCrJ. 2019 Sep 4;6(Pt 6):1014-1023. doi: 10.1107/S205225251901114X. eCollection , 2019 Nov 1. PMID:31709057<ref>PMID:31709057</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5o8t" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Aplca]]
		+	[[Category: Large Structures]]
	[[Category: Dawson, A]]		[[Category: Dawson, A]]
	[[Category: Hunter, W N]]		[[Category: Hunter, W N]]

OCA at 05:24, 27 June 2018

OCA — Wed, 27 Jun 2018 05:24:37 GMT

←Older revision		Revision as of 05:24, 27 June 2018
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-	'''Unreleased structure'''

-	~~The entry 5o8t is ON HOLD until Paper Publication~~	+	==Crystal structure of wild type Aplysia californica AChBP in complex with strychnine==
-		+	<StructureSection load='5o8t' size='340' side='right' caption='[[5o8t]], [[Resolution\|resolution]] 2.20Å' scene=''>
-	~~Authors: Dawson, A., Hunter, W.N., de Souza, J.O., Trumper, P.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5o8t]] is a 5 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5O8T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5O8T FirstGlance]. <br>
-	~~Description:~~ Crystal structure of wild type Aplysia californica AChBP in complex with strychnine	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=SY9:STRYCHNINE'>SY9</scene></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5o8t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5o8t OCA], [http://pdbe.org/5o8t PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5o8t RCSB], [http://www.ebi.ac.uk/pdbsum/5o8t PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5o8t ProSAT]</span></td></tr>
-	[[~~Category~~: ~~Hunter~~, W.N]]	+	</table>
-	[[~~Category~~: ~~De Souza~~, J.O]]	+	__TOC__
		+	</StructureSection>
	[[Category: Dawson, A]]		[[Category: Dawson, A]]
		+	[[Category: Hunter, W N]]
		+	[[Category: Souza, J O.de]]
	[[Category: Trumper, P]]		[[Category: Trumper, P]]
		+	[[Category: Receptor]]
		+	[[Category: Signaling protein]]

OCA: Protected "5o8t" [edit=sysop:move=sysop]

OCA — Wed, 27 Sep 2017 12:10:04 GMT

Protected "5o8t" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:10, 27 September 2017

OCA: New page: '''Unreleased structure''' The entry 5o8t is ON HOLD until Paper Publication Authors: Dawson, A., Hunter, W.N., de Souza, J.O., Trumper, P. Description: Crystal structure of wild type ...

OCA — Wed, 27 Sep 2017 12:10:02 GMT

New page: '''Unreleased structure''' The entry 5o8t is ON HOLD until Paper Publication Authors: Dawson, A., Hunter, W.N., de Souza, J.O., Trumper, P. Description: Crystal structure of wild type ...

New page

'''Unreleased structure'''

The entry 5o8t is ON HOLD until Paper Publication

Authors: Dawson, A., Hunter, W.N., de Souza, J.O., Trumper, P.

Description: Crystal structure of wild type Aplysia californica AChBP in complex with strychnine
[[Category: Unreleased Structures]]
[[Category: Hunter, W.N]]
[[Category: De Souza, J.O]]
[[Category: Dawson, A]]
[[Category: Trumper, P]]