5ob9 - Revision history

OCA at 09:38, 6 December 2023

OCA — Wed, 06 Dec 2023 09:38:58 GMT

←Older revision		Revision as of 09:38, 6 December 2023
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	<StructureSection load='5ob9' size='340' side='right'caption='[[5ob9]], [[Resolution\|resolution]] 2.38Å' scene=''>		<StructureSection load='5ob9' size='340' side='right'caption='[[5ob9]], [[Resolution\|resolution]] 2.38Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5ob9]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OB9 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5OB9 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5ob9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OB9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5OB9 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=9Q8:tris(oxidaniumylidynemethyl)-tris(oxidanyl)ruthenium'>9Q8</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.38Å</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=9Q8:tris(oxidaniumylidynemethyl)-tris(oxidanyl)ruthenium'>9Q8</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5ob9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ob9 OCA], [~~http~~://pdbe.org/5ob9 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5ob9 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5ob9 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5ob9 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ob9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ob9 OCA], [https://pdbe.org/5ob9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ob9 RCSB], [https://www.ebi.ac.uk/pdbsum/5ob9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ob9 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/LYSC_CHICK LYSC_CHICK]] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.<ref>PMID:22044478</ref>	+	[https://www.uniprot.org/uniprot/LYSC_CHICK LYSC_CHICK] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.<ref>PMID:22044478</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	[[Category: Gallus gallus]]		[[Category: Gallus gallus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: Lysozyme]]~~	+	[[Category: Ferraro G]]
-	[[Category: Ferraro, G]]	+	[[Category: Merlino A]]
-	[[Category: Merlino, A]]	+	[[Category: Pontillo N]]
-	[[Category: Pontillo, N]]	+
-	~~[[Category: Co releasing molecule]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Protein-metallodrug interaction~~]]	+

OCA at 12:20, 26 August 2020

OCA — Wed, 26 Aug 2020 12:20:20 GMT

←Older revision		Revision as of 12:20, 26 August 2020
Line 1:		Line 1:

	==X-ray structure of the adduct formed upon reaction of lysozyme with the compound fac-[RuII(CO)3Cl2(N3-MIM), MIM=methyl-imidazole (crystals grown using ethylene glycol==		==X-ray structure of the adduct formed upon reaction of lysozyme with the compound fac-[RuII(CO)3Cl2(N3-MIM), MIM=methyl-imidazole (crystals grown using ethylene glycol==
-	<StructureSection load='5ob9' size='340' side='right' caption='[[5ob9]], [[Resolution\|resolution]] 2.38Å' scene=''>	+	<StructureSection load='5ob9' size='340' side='right'caption='[[5ob9]], [[Resolution\|resolution]] 2.38Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5ob9]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OB9 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5OB9 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5ob9]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OB9 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5OB9 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=9Q8:tris(oxidaniumylidynemethyl)-tris(oxidanyl)ruthenium'>9Q8</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=9Q8:tris(oxidaniumylidynemethyl)-tris(oxidanyl)ruthenium'>9Q8</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5ob9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ob9 OCA], [http://pdbe.org/5ob9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ob9 RCSB], [http://www.ebi.ac.uk/pdbsum/5ob9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ob9 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5ob9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ob9 OCA], [http://pdbe.org/5ob9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ob9 RCSB], [http://www.ebi.ac.uk/pdbsum/5ob9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ob9 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 19:		Line 19:
	</div>		</div>
	<div class="pdbe-citations 5ob9" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5ob9" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Lysozyme 3D structures\|Lysozyme 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 24:		Line 27:
	</StructureSection>		</StructureSection>
	[[Category: Gallus gallus]]		[[Category: Gallus gallus]]
		+	[[Category: Large Structures]]
	[[Category: Lysozyme]]		[[Category: Lysozyme]]
	[[Category: Ferraro, G]]		[[Category: Ferraro, G]]

OCA: Protected "5ob9" [edit=sysop:move=sysop]

OCA — Fri, 04 Aug 2017 04:07:09 GMT

Protected "5ob9" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:07, 4 August 2017

OCA: New page: ==X-ray structure of the adduct formed upon reaction of lysozyme with the compound fac-[RuII(CO)3Cl2(N3-MIM), MIM=methyl-imidazole (crystals grown using ethylene glycol==
OCA — Fri, 04 Aug 2017 04:07:07 GMT

New page: ==X-ray structure of the adduct formed upon reaction of lysozyme with the compound fac-[RuII(CO)3Cl2(N3-MIM), MIM=methyl-imidazole (crystals grown using ethylene glycol== <StructureSectio...

New page

==X-ray structure of the adduct formed upon reaction of lysozyme with the compound fac-[RuII(CO)3Cl2(N3-MIM), MIM=methyl-imidazole (crystals grown using ethylene glycol==
<StructureSection load='5ob9' size='340' side='right' caption='[[5ob9]], [[Resolution|resolution]] 2.38Å' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5ob9]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OB9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5OB9 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=9Q8:tris(oxidaniumylidynemethyl)-tris(oxidanyl)ruthenium'>9Q8</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene></td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ob9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ob9 OCA], [http://pdbe.org/5ob9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ob9 RCSB], [http://www.ebi.ac.uk/pdbsum/5ob9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ob9 ProSAT]</span></td></tr>
</table>
== Function ==
[[http://www.uniprot.org/uniprot/LYSC_CHICK LYSC_CHICK]] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.<ref>PMID:22044478</ref>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
fac-[RuII(CO)3Cl2(N3-Imidazole)] (RuIIIM), fac-[RuII(CO)3Cl2(N3-methyl-imidazole)] (RuIIMIM) and fac-[RuII(CO)3Cl2(N3-methyl-benzimidazole)] (RuIIMBI) are three ruthenium based CO releasing molecules (Ru-CORMs) that are cytotoxic towards ovarian and colon carcinoma cell lines. Detailed structural information on the adducts formed upon reaction of RuIIIM and RuIIMIM with hen egg white lysozyme and of the three Ru-CORMs with bovine pancreatic ribonuclease is provided here by X-ray crystallography. Comparative analysis of seven crystal structures of these adducts allows one to delineate some general trends in the reactivity of these Ru-CORMs with proteins. Indeed, in all cases Ru-CORMs bind these model systems upon detachment of the azole ligand and concomitant coordination to a protein His or Asp residue. Apparently the three Ru-CORMs progressively dissociate losing azoles, chlorides, and one or two CO molecules. Data were compared with those reported in the literature for adducts of the same proteins with other Ru-CORMs and with in-solution data previously obtained on the same systems. These results are potentially useful for a better understanding of the chemistry, potential toxicity and mechanism of actions of these interesting Ru-CORMs and are helpful in defining the molecular mechanisms of CO release.

Ru-Based CO releasing molecules with azole ligands: interaction with proteins and the CO release mechanism disclosed by X-ray crystallography.,Pontillo N, Ferraro G, Messori L, Tamasi G, Merlino A Dalton Trans. 2017 Jul 25;46(29):9621-9629. doi: 10.1039/c7dt01991b. PMID:28702564<ref>PMID:28702564</ref>

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5ob9" style="background-color:#fffaf0;"></div>
== References ==
<references/>
TOC
</StructureSection>
[[Category: Gallus gallus]]
[[Category: Lysozyme]]
[[Category: Ferraro, G]]
[[Category: Merlino, A]]
[[Category: Pontillo, N]]
[[Category: Co releasing molecule]]
[[Category: Hydrolase]]
[[Category: Protein-metallodrug interaction]]