5obx - Revision history

OCA at 09:39, 6 December 2023

OCA — Wed, 06 Dec 2023 09:39:26 GMT

←Older revision		Revision as of 09:39, 6 December 2023
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	<StructureSection load='5obx' size='340' side='right'caption='[[5obx]], [[Resolution\|resolution]] 1.78Å' scene=''>		<StructureSection load='5obx' size='340' side='right'caption='[[5obx]], [[Resolution\|resolution]] 1.78Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5obx]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OBX OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5OBX FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5obx]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycoplasma_genitalium_G37 Mycoplasma genitalium G37]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OBX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5OBX FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.78Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5obx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5obx OCA], [~~http~~://pdbe.org/5obx PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5obx RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5obx PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5obx ProSAT]</span></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5obx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5obx OCA], [https://pdbe.org/5obx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5obx RCSB], [https://www.ebi.ac.uk/pdbsum/5obx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5obx ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/DNAK_MYCGE DNAK_MYCGE]] Acts as a chaperone.	+	[https://www.uniprot.org/uniprot/DNAK_MYCGE DNAK_MYCGE] Acts as a chaperone.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Adell, M]]	+	[[Category: Mycoplasma genitalium G37]]
-	[[Category: Calisto, B]]	+	[[Category: Adell M]]
-	[[Category: Fita, I]]	+	[[Category: Calisto B]]
-	[[Category: Martinelli, L]]	+	[[Category: Fita I]]
-	~~[[Category: Atp hydrolysis]]~~	+	[[Category: Martinelli L]]
-	~~[[Category: Chaperone]]~~	+
-	~~[[Category: Co-factor]]~~	+
-	~~[[Category: Complex~~]]	+

OCA at 12:20, 26 August 2020

OCA — Wed, 26 Aug 2020 12:20:57 GMT

←Older revision		Revision as of 12:20, 26 August 2020
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	==Mycoplasma genitalium DnaK-NBD==		==Mycoplasma genitalium DnaK-NBD==
-	<StructureSection load='5obx' size='340' side='right' caption='[[5obx]], [[Resolution\|resolution]] 1.78Å' scene=''>	+	<StructureSection load='5obx' size='340' side='right'caption='[[5obx]], [[Resolution\|resolution]] 1.78Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5obx]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OBX OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5OBX FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5obx]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OBX OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5OBX FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5obx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5obx OCA], [http://pdbe.org/5obx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5obx RCSB], [http://www.ebi.ac.uk/pdbsum/5obx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5obx ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5obx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5obx OCA], [http://pdbe.org/5obx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5obx RCSB], [http://www.ebi.ac.uk/pdbsum/5obx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5obx ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	</div>		</div>
	<div class="pdbe-citations 5obx" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5obx" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Heat Shock Protein structures\|Heat Shock Protein structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Adell, M]]		[[Category: Adell, M]]
	[[Category: Calisto, B]]		[[Category: Calisto, B]]

OCA at 06:08, 9 May 2018

OCA — Wed, 09 May 2018 06:08:42 GMT

←Older revision		Revision as of 06:08, 9 May 2018
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-	'''Unreleased structure'''

-	~~The entry~~ 5obx is ~~ON HOLD~~	+	==Mycoplasma genitalium DnaK-NBD==
		+	<StructureSection load='5obx' size='340' side='right' caption='[[5obx]], [[Resolution\|resolution]] 1.78Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5obx]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OBX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5OBX FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5obx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5obx OCA], [http://pdbe.org/5obx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5obx RCSB], [http://www.ebi.ac.uk/pdbsum/5obx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5obx ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/DNAK_MYCGE DNAK_MYCGE]] Acts as a chaperone.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Hsp70 chaperones keep protein homeostasis facilitating the response of organisms to changes in external and internal conditions. Hsp70s have two domains-nucleotide binding domain (NBD) and substrate binding domain (SBD)-connected by a conserved hydrophobic linker. Functioning of Hsp70s depend on tightly regulated cycles of ATP hydrolysis allosterically coupled, often together with cochaperones, to the binding/release of peptide substrates. Here we describe the crystal structure of the Mycoplasma genitalium DnaK (MgDnaK) protein, an Hsp70 homolog, in the noncompact, nucleotide-bound/substrate-bound conformation. The MgDnaK structure resembles the one from the thermophilic eubacteria DnaK trapped in the same state. However, in MgDnaK the NBD and SBD domains remain close to each other despite the lack of direct interaction between them and with the linker contacting the two subdomains of SBD. These observations suggest that the structures might represent an intermediate of the protein where the conserved linker binds to the SBD to favor the noncompact state of the protein by stabilizing the SBDbeta-SBDalpha subdomains interaction, promoting the capacity of the protein to sample different conformations, which is critical for proper functioning of the molecular chaperone allosteric mechanism. Comparison of the solved structures indicates that the NBD remains essentially invariant in presence or absence of nucleotide.

-	~~Authors: Adell~~, M., Calisto~~, B.~~, Fita, I., Martinelli, L.	+	The nucleotide-bound/substrate-bound conformation of the Mycoplasma genitalium DnaK chaperone.,Adell M, Calisto BM, Fita I, Martinelli L Protein Sci. 2018 May;27(5):1000-1007. doi: 10.1002/pro.3401. Epub 2018 Apr 14. PMID:29520883<ref>PMID:29520883</ref>

-	~~Description: Mycoplasma genitalium DnaK-NBD~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category: Unreleased Structures]]~~	+	</div>
-	~~[[Category~~: ~~Calisto, B]]~~	+	<div class="pdbe-citations 5obx" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Adell, M]]		[[Category: Adell, M]]
-	[[Category: ~~Martinelli~~, L]]	+	[[Category: Calisto, B]]
	[[Category: Fita, I]]		[[Category: Fita, I]]
		+	[[Category: Martinelli, L]]
		+	[[Category: Atp hydrolysis]]
		+	[[Category: Chaperone]]
		+	[[Category: Co-factor]]
		+	[[Category: Complex]]

OCA at 03:33, 8 March 2018

OCA — Thu, 08 Mar 2018 03:33:25 GMT

←Older revision		Revision as of 03:33, 8 March 2018
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5obx is ON HOLD ~~until Paper Publication~~	+	The entry 5obx is ON HOLD

	Authors: Adell, M., Calisto, B., Fita, I., Martinelli, L.		Authors: Adell, M., Calisto, B., Fita, I., Martinelli, L.
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	Description: Mycoplasma genitalium DnaK-NBD		Description: Mycoplasma genitalium DnaK-NBD
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Martinelli, L]]
	[[Category: Calisto, B]]		[[Category: Calisto, B]]
	[[Category: Adell, M]]		[[Category: Adell, M]]
		+	[[Category: Martinelli, L]]
	[[Category: Fita, I]]		[[Category: Fita, I]]

OCA: Protected "5obx" [edit=sysop:move=sysop]

OCA — Wed, 27 Sep 2017 12:15:31 GMT

Protected "5obx" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:15, 27 September 2017

OCA: New page: '''Unreleased structure''' The entry 5obx is ON HOLD until Paper Publication Authors: Adell, M., Calisto, B., Fita, I., Martinelli, L. Description: Mycoplasma genitalium DnaK-NBD [[Cat...

OCA — Wed, 27 Sep 2017 12:15:30 GMT

New page: '''Unreleased structure''' The entry 5obx is ON HOLD until Paper Publication Authors: Adell, M., Calisto, B., Fita, I., Martinelli, L. Description: Mycoplasma genitalium DnaK-NBD [[Cat...

New page

'''Unreleased structure'''

The entry 5obx is ON HOLD until Paper Publication

Authors: Adell, M., Calisto, B., Fita, I., Martinelli, L.

Description: Mycoplasma genitalium DnaK-NBD
[[Category: Unreleased Structures]]
[[Category: Martinelli, L]]
[[Category: Calisto, B]]
[[Category: Adell, M]]
[[Category: Fita, I]]