5oe6 - Revision history

OCA at 16:46, 13 December 2023

OCA — Wed, 13 Dec 2023 16:46:30 GMT

←Older revision		Revision as of 16:46, 13 December 2023
Line 3:		Line 3:
	<StructureSection load='5oe6' size='340' side='right'caption='[[5oe6]], [[Resolution\|resolution]] 1.67Å' scene=''>		<StructureSection load='5oe6' size='340' side='right'caption='[[5oe6]], [[Resolution\|resolution]] 1.67Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5oe6]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OE6 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5OE6 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5oe6]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OE6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5OE6 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=9SN:6~~-~~fluoroanthraniloyl-AMP'>9SN</scene>~~, ~~<scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.67Å</td></tr>
-	<tr id='~~activity~~'><td class="sblockLbl"><b>~~Activity~~:</b></td><td class="sblockDat"><~~span class~~='~~plainlinks~~'>~~[http:~~/~~/en.wikipedia.org/wiki/Ligase Ligase]~~, ~~with EC number [http~~:~~//www.brenda-enzymes.info/php/result_flat.php4?ecno=6.2.1.32 6.2.1.32]~~ </~~span~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=9SN:6-fluoroanthraniloyl-AMP'>9SN</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5oe6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5oe6 OCA], [~~http~~://pdbe.org/5oe6 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5oe6 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5oe6 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5oe6 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5oe6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5oe6 OCA], [https://pdbe.org/5oe6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5oe6 RCSB], [https://www.ebi.ac.uk/pdbsum/5oe6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5oe6 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/PQSA_PSEAE PQSA_PSEAE]] Catalyzes the formation of anthraniloyl-CoA, which is the priming step for entry into the Pseudomonas quinolone signal (PQS) biosynthetic pathway. Also active on a variety of aromatic substrates, including benzoate and chloro and fluoro derivatives of anthranilate.<ref>PMID:18083812</ref> <ref>PMID:18728009</ref>	+	[https://www.uniprot.org/uniprot/PQSA_PSEAE PQSA_PSEAE] Catalyzes the formation of anthraniloyl-CoA, which is the priming step for entry into the Pseudomonas quinolone signal (PQS) biosynthetic pathway. Also active on a variety of aromatic substrates, including benzoate and chloro and fluoro derivatives of anthranilate.<ref>PMID:18083812</ref> <ref>PMID:18728009</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 24:		Line 24:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Ligase~~]]	+	[[Category: Pseudomonas aeruginosa PAO1]]
-	[[Category: Blankenfeldt, W]]	+	[[Category: Blankenfeldt W]]
-	[[Category: Ewert, W]]	+	[[Category: Ewert W]]
-	[[Category: Witzgall, F]]	+	[[Category: Witzgall F]]
-	~~[[Category: 6-fluoroanthranilate]]~~	+
-	~~[[Category: 6-fluoroanthraniloyl-amp]]~~	+
-	~~[[Category: 6faba-amp]]~~	+
-	~~[[Category: Anl superfamily]]~~	+
-	~~[[Category: Anthranilate]]~~	+
-	~~[[Category: Anthraniloyl-amp]]~~	+
-	~~[[Category: Anthraniloyl-coa]]~~	+
-	~~[[Category: Aryl-coa ligase]]~~	+
-	~~[[Category: Fluor]]~~	+
-	~~[[Category: Pq]]~~	+
-	~~[[Category: Pqsa]]~~	+
-	~~[[Category: Pseudomonas aeruginosa]]~~	+
-	~~[[Category: Pseudomonas quinolone signal]]~~	+
-	~~[[Category: Quorum sensing~~]]	+

OCA at 12:22, 26 August 2020

OCA — Wed, 26 Aug 2020 12:22:04 GMT

←Older revision		Revision as of 12:22, 26 August 2020
Line 1:		Line 1:

	==Crystal structure of the N-terminal domain of PqsA in complex with 6-fluoroanthraniloyl-AMP (crystal form 1)==		==Crystal structure of the N-terminal domain of PqsA in complex with 6-fluoroanthraniloyl-AMP (crystal form 1)==
-	<StructureSection load='5oe6' size='340' side='right' caption='[[5oe6]], [[Resolution\|resolution]] 1.67Å' scene=''>	+	<StructureSection load='5oe6' size='340' side='right'caption='[[5oe6]], [[Resolution\|resolution]] 1.67Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5oe6]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OE6 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5OE6 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5oe6]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OE6 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5OE6 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=9SN:6-fluoroanthraniloyl-AMP'>9SN</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=9SN:6-fluoroanthraniloyl-AMP'>9SN</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ligase Ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.2.1.32 6.2.1.32] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ligase Ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.2.1.32 6.2.1.32] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5oe6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5oe6 OCA], [http://pdbe.org/5oe6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5oe6 RCSB], [http://www.ebi.ac.uk/pdbsum/5oe6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5oe6 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5oe6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5oe6 OCA], [http://pdbe.org/5oe6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5oe6 RCSB], [http://www.ebi.ac.uk/pdbsum/5oe6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5oe6 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 23:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Ligase]]		[[Category: Ligase]]
	[[Category: Blankenfeldt, W]]		[[Category: Blankenfeldt, W]]

OCA: Protected "5oe6" [edit=sysop:move=sysop]

OCA — Wed, 06 Sep 2017 03:39:46 GMT

Protected "5oe6" [edit=sysop:move=sysop]

←Older revision

Revision as of 03:39, 6 September 2017

OCA: New page: ==Crystal structure of the N-terminal domain of PqsA in complex with 6-fluoroanthraniloyl-AMP (crystal form 1)== == Structural highlights ==
<table><tr><td colspan='2'>[[5oe6]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OE6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5OE6 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=9SN:6-fluoroanthraniloyl-AMP'>9SN</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ligase Ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.2.1.32 6.2.1.32] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5oe6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5oe6 OCA], [http://pdbe.org/5oe6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5oe6 RCSB], [http://www.ebi.ac.uk/pdbsum/5oe6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5oe6 ProSAT]</span></td></tr>
</table>
== Function ==
[[http://www.uniprot.org/uniprot/PQSA_PSEAE PQSA_PSEAE]] Catalyzes the formation of anthraniloyl-CoA, which is the priming step for entry into the Pseudomonas quinolone signal (PQS) biosynthetic pathway. Also active on a variety of aromatic substrates, including benzoate and chloro and fluoro derivatives of anthranilate.<ref>PMID:18083812</ref> <ref>PMID:18728009</ref>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Pseudomonas aeruginosa, a prevalent pathogen in nosocomial infections and a major burden in cystic fibrosis, uses three interconnected quorum-sensing systems to coordinate virulence processes. At variance with other Gram-negatives, one of these systems relies on alkylquinolones (Pseudomonas Quinolone Signal, PQS) and may hence be an attractive target for new anti-infectives. Here, we report crystal structures of the N-terminal domain of anthranilate-CoA ligase PqsA, the first enzyme of PQS biosynthesis, in complex with anthraniloyl-AMP and 6-fluoroanthraniloyl-AMP (6FABA-AMP) at 1.4 and 1.7 A resolution. We find that PqsA belongs to an unrecognized subfamily of anthranilate-CoA ligases that recognizes the amino group of anthranilate through a water-mediated hydrogen bond. The complex with 6FABA-AMP explains why 6FABA, an inhibitor of PQS biosynthesis, is a good substrate of PqsA. Together, our data may pave a way to new pathoblockers in P. aeruginosa infections.

Structures of the N-terminal domain of PqsA in complex with anthraniloyl- and 6-fluoroanthraniloyl-AMP: substrate activation in Pseudomonas Quinolone Signal (PQS) biosynthesis.,Witzgall F, Ewert W, Blankenfeldt W Chembiochem. 2017 Aug 18. doi: 10.1002/cbic.201700374. PMID:28834007<ref>PMID:28834007</ref>

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5oe6" style="background-color:#fffaf0;"></div>
== References ==
<references/>
TOC
</StructureSection>
[[Category: Ligase]]
[[Category: Blankenfeldt, W]]
[[Category: Ewert, W]]
[[Category: Witzgall, F]]
[[Category: 6-fluoroanthranilate]]
[[Category: 6-fluoroanthraniloyl-amp]]
[[Category: 6faba-amp]]
[[Category: Anl superfamily]]
[[Category: Anthranilate]]
[[Category: Anthraniloyl-amp]]
[[Category: Anthraniloyl-coa]]
[[Category: Aryl-coa ligase]]
[[Category: Fluor]]
[[Category: Pq]]
[[Category: Pqsa]]
[[Category: Pseudomonas aeruginosa]]
[[Category: Pseudomonas quinolone signal]]
[[Category: Quorum sensing]]