5ojm - Revision history

OCA at 16:52, 13 December 2023

OCA — Wed, 13 Dec 2023 16:52:13 GMT

←Older revision		Revision as of 16:52, 13 December 2023
Line 1:		Line 1:

	==Structure of a chimaeric beta3-alpha5 GABAA receptor in complex with nanobody Nb25==		==Structure of a chimaeric beta3-alpha5 GABAA receptor in complex with nanobody Nb25==
-	<StructureSection load='5ojm' size='340' side='right' caption='[[5ojm]], [[Resolution\|resolution]] 3.30Å' scene=''>	+	<StructureSection load='5ojm' size='340' side='right'caption='[[5ojm]], [[Resolution\|resolution]] 3.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5ojm]] is a 10 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Camelus_glama Camelus~~ glama] and [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OJM OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5OJM FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5ojm]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens], [https://en.wikipedia.org/wiki/Lama_glama Lama glama] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OJM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5OJM FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.3Å</td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GABRA5 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5ojm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ojm OCA], [~~http~~://pdbe.org/5ojm PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5ojm RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5ojm PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5ojm ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ojm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ojm OCA], [https://pdbe.org/5ojm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ojm RCSB], [https://www.ebi.ac.uk/pdbsum/5ojm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ojm ProSAT]</span></td></tr>
	</table>		</table>
		+	== Disease ==
		+	[https://www.uniprot.org/uniprot/GBRB3_HUMAN GBRB3_HUMAN] Autism;Childhood absence epilepsy. Disease susceptibility is associated with variations affecting the gene represented in this entry.
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/GBRB3_HUMAN GBRB3_HUMAN] GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel.[https://www.uniprot.org/uniprot/GBRA5_HUMAN GBRA5_HUMAN] GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 25:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Camelus glama~~]]	+	[[Category: Homo sapiens]]
-	[[Category: ~~Human~~]]	+	[[Category: Lama glama]]
-	[[Category: ~~Aricescu, A R~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Colibus, L De~~]]	+	[[Category: Synthetic construct]]
-	[[Category: ~~Masiulis, S~~]]	+	[[Category: Aricescu AR]]
-	[[Category: ~~Miller, P S~~]]	+	[[Category: De Colibus L]]
-	[[Category: ~~Pardon, E]]~~	+	[[Category: Masiulis S]]
-	~~[[Category: Scott,~~ S]]	+	[[Category: Miller PS]]
-	[[Category: ~~Steyaert, J~~]]	+	[[Category: Pardon E]]
-	[[Category: ~~Gabaa receptor~~]]	+	[[Category: Scott S]]
-	[[Category: ~~Ion channel~~]]	+	[[Category: Steyaert J]]
-	[[Category: ~~Membrane protein]]~~	+
-	~~[[Category: Nanobody complex]]~~	+
-	~~[[Category: Neurosteroid~~]]	+

OCA at 10:13, 15 November 2017

OCA — Wed, 15 Nov 2017 10:13:42 GMT

←Older revision		Revision as of 10:13, 15 November 2017
Line 3:		Line 3:
	<StructureSection load='5ojm' size='340' side='right' caption='[[5ojm]], [[Resolution\|resolution]] 3.30Å' scene=''>		<StructureSection load='5ojm' size='340' side='right' caption='[[5ojm]], [[Resolution\|resolution]] 3.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5ojm]] is a 10 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OJM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5OJM FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5ojm]] is a 10 chain structure with sequence from [http://en.wikipedia.org/wiki/Camelus_glama Camelus glama] and [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OJM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5OJM FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GABRA5 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ojm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ojm OCA], [http://pdbe.org/5ojm PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ojm RCSB], [http://www.ebi.ac.uk/pdbsum/5ojm PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ojm ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ojm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ojm OCA], [http://pdbe.org/5ojm PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ojm RCSB], [http://www.ebi.ac.uk/pdbsum/5ojm PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ojm ProSAT]</span></td></tr>
	</table>		</table>
Line 20:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Camelus glama]]
		+	[[Category: Human]]
	[[Category: Aricescu, A R]]		[[Category: Aricescu, A R]]
	[[Category: Colibus, L De]]		[[Category: Colibus, L De]]

OCA at 06:56, 25 October 2017

OCA — Wed, 25 Oct 2017 06:56:11 GMT

←Older revision		Revision as of 06:56, 25 October 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5ojm is ~~ON HOLD~~	+	==Structure of a chimaeric beta3-alpha5 GABAA receptor in complex with nanobody Nb25==
		+	<StructureSection load='5ojm' size='340' side='right' caption='[[5ojm]], [[Resolution\|resolution]] 3.30Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5ojm]] is a 10 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OJM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5OJM FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ojm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ojm OCA], [http://pdbe.org/5ojm PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ojm RCSB], [http://www.ebi.ac.uk/pdbsum/5ojm PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ojm ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Type A gamma-aminobutyric acid receptors (GABAARs) are the principal mediators of inhibitory neurotransmission in the human brain. Endogenous neurosteroids interact with GABAARs to regulate acute and chronic anxiety and are potent sedative, analgesic, anticonvulsant and anesthetic agents. Their mode of binding and mechanism of receptor potentiation, however, remain unknown. Here we report crystal structures of a chimeric GABAAR construct in apo and pregnanolone-bound states. The neurosteroid-binding site is mechanically coupled to the helices lining the ion channel pore and modulates the desensitization-gate conformation. We demonstrate that the equivalent site is responsible for physiological, heteromeric GABAAR potentiation and explain the contrasting modulatory properties of 3a versus 3b neurosteroid epimers. These results illustrate how peripheral lipid ligands can regulate the desensitization gate of GABAARs, a process of broad relevance to pentameric ligand-gated ion channels.

-	~~Authors:~~ Miller~~, P.S.~~, Scott, S., Masiulis, S., De Colibus, L., Pardon, E., Steyaert, J., Aricescu~~, A~~.R.	+	Structural basis for GABAA receptor potentiation by neurosteroids.,Miller PS, Scott S, Masiulis S, De Colibus L, Pardon E, Steyaert J, Aricescu AR Nat Struct Mol Biol. 2017 Oct 9. doi: 10.1038/nsmb.3484. PMID:28991263<ref>PMID:28991263</ref>

-	~~Description: Structure of~~ a ~~chimaeric beta3~~-~~alpha5 GABAA receptor in complex with nanobody Nb25~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 5ojm" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Aricescu, A R]]
		+	[[Category: Colibus, L De]]
	[[Category: Masiulis, S]]		[[Category: Masiulis, S]]
-	[[Category: Miller, P.S]]	+	[[Category: Miller, P S]]
-	~~[[Category: De Colibus, L~~]]	+
	[[Category: Pardon, E]]		[[Category: Pardon, E]]
-	[[Category: Aricescu, A.R]]
-	[[Category: Steyaert, J]]
	[[Category: Scott, S]]		[[Category: Scott, S]]
		+	[[Category: Steyaert, J]]
		+	[[Category: Gabaa receptor]]
		+	[[Category: Ion channel]]
		+	[[Category: Membrane protein]]
		+	[[Category: Nanobody complex]]
		+	[[Category: Neurosteroid]]

OCA: Protected "5ojm" [edit=sysop:move=sysop]

OCA — Wed, 27 Sep 2017 12:30:32 GMT

Protected "5ojm" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:30, 27 September 2017

OCA: New page: '''Unreleased structure''' The entry 5ojm is ON HOLD Authors: Miller, P.S., Scott, S., Masiulis, S., De Colibus, L., Pardon, E., Steyaert, J., Aricescu, A.R. Description: Structure of ...

OCA — Wed, 27 Sep 2017 12:30:31 GMT

New page: '''Unreleased structure''' The entry 5ojm is ON HOLD Authors: Miller, P.S., Scott, S., Masiulis, S., De Colibus, L., Pardon, E., Steyaert, J., Aricescu, A.R. Description: Structure of ...

New page

'''Unreleased structure'''

The entry 5ojm is ON HOLD

Authors: Miller, P.S., Scott, S., Masiulis, S., De Colibus, L., Pardon, E., Steyaert, J., Aricescu, A.R.

Description: Structure of a chimaeric beta3-alpha5 GABAA receptor in complex with nanobody Nb25
[[Category: Unreleased Structures]]
[[Category: Masiulis, S]]
[[Category: Miller, P.S]]
[[Category: De Colibus, L]]
[[Category: Pardon, E]]
[[Category: Aricescu, A.R]]
[[Category: Steyaert, J]]
[[Category: Scott, S]]