5oli - Revision history

OCA: Redirecting to 6qe4

OCA — Wed, 13 Mar 2019 11:46:56 GMT

Redirecting to 6qe4

←Older revision		Revision as of 11:46, 13 March 2019
Line 1:		Line 1:
-		+	#REDIRECT [[6qe4]] This PDB entry is obsolete and replaced by 6qe4
-	~~==Crystal structure of human IBA57==~~	+
-	~~<StructureSection load='5oli' size='340' side='right' caption='~~[[~~5oli~~]]~~, [[Resolution\|resolution]] 2.30Å' scene=''>~~	+
-	~~== Structural highlights ==~~	+
-	<table><tr><td colspan='2'>[[5oli]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OLI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5OLI FirstGlance]. <br>	+
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=I3C:5-AMINO-2,4,6-TRIIODOBENZENE-1,3-DICARBOXYLIC+ACID'>I3C</scene></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5oli FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5oli OCA], [http://pdbe.org/5oli PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5oli RCSB], [http://www.ebi.ac.uk/pdbsum/5oli PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5oli ProSAT]</span></td></tr>	+
-	~~</table>~~	+
-	~~== Disease ==~~	+
-	~~[[http://www.uniprot.org/uniprot/CAF17_HUMAN CAF17_HUMAN]] Hypotonia-cerebral atrophy-hyperglycinemia syndrome. The disease is caused by mutations affecting the gene represented in this~~ entry~~. The disease~~ is ~~caused by mutations affecting the gene represented in this entry.~~	+
-	~~== Function ==~~	+
-	[[http://www.uniprot.org/uniprot/CAF17_HUMAN CAF17_HUMAN]] Involved in the maturation of mitochondrial 4Fe-4S proteins functioning late in the iron-sulfur cluster assembly pathway.<ref>PMID:23462291</ref>	+
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	~~The maturation of mitochondrial iron-sulfur proteins requires a complex protein machinery. Human IBA57 protein was proposed to act in a late phase of this machinery, along with GLRX5, ISCA1,~~ and ~~ISCA2. However, a molecular picture on how these proteins cooperate is not defined yet. We show here that IBA57 forms a heterodimeric complex with ISCA2~~ by bridging a [2Fe-2S] cluster, that [2Fe-2S] cluster binding is absolutely required to promote the complex formation, and that the cysteine of the conserved motif characterizing IBA57 protein family and the three conserved cysteines of the ISCA protein family act as cluster ligands. The [2Fe-2S] heterodimeric complex is the final product when IBA57 is either exposed to [2Fe-2S] ISCA2 or in the presence of [2Fe-2S] GLRX5 and apo ISCA2. We also find that the [2Fe-2S] ISCA2-IBA57 complex is resistant to highly oxidative environments and is capable of reactivating apo aconitase in vitro. Collectively, our data delinate a [2Fe-2S] cluster transfer pathway involving three partner proteins of the mitochondrial ISC machinery, that is, GLRX5, ISCA2 and IBA57, which leads to the formation of a [2Fe-2S] ISCA2-IBA57 complex.	+
-		+
-	IBA57 Recruits ISCA2 to Form a [2Fe-2S] Cluster-Mediated Complex.,Gourdoupis S, Nasta V, Calderone V, Ciofi-Baffoni S, Banci L J Am Chem Soc. 2018 Oct 31;140(43):14401-14412. doi: 10.1021/jacs.8b09061. Epub, 2018 Oct 17. PMID:30269484<ref>PMID:30269484</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 5oli" style="background-color:#fffaf0;"></div>~~	+
-	~~== References ==~~	+
-	~~<references/>~~	+
-	~~__TOC__~~	+
-	~~</StructureSection>~~	+
-	~~[[Category: Calderone, V]]~~	+
-	~~[[Category: Ciofi-Baffoni, S]]~~	+
-	~~[[Category: Gourdoupis, S]]~~	+
-	~~[[Category: Fe-s protein biogenesis]]~~	+
-	~~[[Category: I3c phasing]]~~	+
-	~~[[Category: Infantile leukodystrophy]]~~	+
-	~~[[Category: Mitochondrial protein]]~~	+
-	~~[[Category: Protein binding]]~~	+

OCA at 12:07, 7 November 2018

OCA — Wed, 07 Nov 2018 12:07:24 GMT

←Older revision		Revision as of 12:07, 7 November 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	The entry ~~5oli~~ is ~~ON HOLD~~ ~~until Paper~~ Publication	+	==Crystal structure of human IBA57==
		+	<StructureSection load='5oli' size='340' side='right' caption='[[5oli]], [[Resolution\|resolution]] 2.30Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5oli]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OLI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5OLI FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=I3C:5-AMINO-2,4,6-TRIIODOBENZENE-1,3-DICARBOXYLIC+ACID'>I3C</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5oli FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5oli OCA], [http://pdbe.org/5oli PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5oli RCSB], [http://www.ebi.ac.uk/pdbsum/5oli PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5oli ProSAT]</span></td></tr>
		+	</table>
		+	== Disease ==
		+	[[http://www.uniprot.org/uniprot/CAF17_HUMAN CAF17_HUMAN]] Hypotonia-cerebral atrophy-hyperglycinemia syndrome. The disease is caused by mutations affecting the gene represented in this entry. The disease is caused by mutations affecting the gene represented in this entry.
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/CAF17_HUMAN CAF17_HUMAN]] Involved in the maturation of mitochondrial 4Fe-4S proteins functioning late in the iron-sulfur cluster assembly pathway.<ref>PMID:23462291</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The maturation of mitochondrial iron-sulfur proteins requires a complex protein machinery. Human IBA57 protein was proposed to act in a late phase of this machinery, along with GLRX5, ISCA1, and ISCA2. However, a molecular picture on how these proteins cooperate is not defined yet. We show here that IBA57 forms a heterodimeric complex with ISCA2 by bridging a [2Fe-2S] cluster, that [2Fe-2S] cluster binding is absolutely required to promote the complex formation, and that the cysteine of the conserved motif characterizing IBA57 protein family and the three conserved cysteines of the ISCA protein family act as cluster ligands. The [2Fe-2S] heterodimeric complex is the final product when IBA57 is either exposed to [2Fe-2S] ISCA2 or in the presence of [2Fe-2S] GLRX5 and apo ISCA2. We also find that the [2Fe-2S] ISCA2-IBA57 complex is resistant to highly oxidative environments and is capable of reactivating apo aconitase in vitro. Collectively, our data delinate a [2Fe-2S] cluster transfer pathway involving three partner proteins of the mitochondrial ISC machinery, that is, GLRX5, ISCA2 and IBA57, which leads to the formation of a [2Fe-2S] ISCA2-IBA57 complex.

-	~~Authors: Calderone~~, V., Ciofi-Baffoni, S., ~~Gourdoupis, S~~.	+	IBA57 Recruits ISCA2 to Form a [2Fe-2S] Cluster-Mediated Complex.,Gourdoupis S, Nasta V, Calderone V, Ciofi-Baffoni S, Banci L J Am Chem Soc. 2018 Oct 31;140(43):14401-14412. doi: 10.1021/jacs.8b09061. Epub, 2018 Oct 17. PMID:30269484<ref>PMID:30269484</ref>

-	~~Description: Crystal structure~~ of ~~human IBA57~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category~~: ~~Unreleased Structures]]~~	+	</div>
		+	<div class="pdbe-citations 5oli" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Calderone, V]]		[[Category: Calderone, V]]
-	[[Category: Gourdoupis, S]]
	[[Category: Ciofi-Baffoni, S]]		[[Category: Ciofi-Baffoni, S]]
		+	[[Category: Gourdoupis, S]]
		+	[[Category: Fe-s protein biogenesis]]
		+	[[Category: I3c phasing]]
		+	[[Category: Infantile leukodystrophy]]
		+	[[Category: Mitochondrial protein]]
		+	[[Category: Protein binding]]

OCA: Protected "5oli" [edit=sysop:move=sysop]

OCA — Wed, 27 Sep 2017 12:33:40 GMT

Protected "5oli" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:33, 27 September 2017

OCA: New page: '''Unreleased structure''' The entry 5oli is ON HOLD until Paper Publication Authors: Calderone, V., Ciofi-Baffoni, S., Gourdoupis, S. Description: Crystal structure of human IBA57 [[C...

OCA — Wed, 27 Sep 2017 12:33:39 GMT

New page: '''Unreleased structure''' The entry 5oli is ON HOLD until Paper Publication Authors: Calderone, V., Ciofi-Baffoni, S., Gourdoupis, S. Description: Crystal structure of human IBA57 [[C...

New page

'''Unreleased structure'''

The entry 5oli is ON HOLD until Paper Publication

Authors: Calderone, V., Ciofi-Baffoni, S., Gourdoupis, S.

Description: Crystal structure of human IBA57
[[Category: Unreleased Structures]]
[[Category: Calderone, V]]
[[Category: Gourdoupis, S]]
[[Category: Ciofi-Baffoni, S]]