5opo - Revision history

OCA at 16:57, 13 December 2023

OCA — Wed, 13 Dec 2023 16:57:37 GMT

←Older revision		Revision as of 16:57, 13 December 2023
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	<StructureSection load='5opo' size='340' side='right'caption='[[5opo]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='5opo' size='340' side='right'caption='[[5opo]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5opo]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OPO OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5OPO FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5opo]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OPO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5OPO FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat"~~><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
-	~~<tr~~ id~~='related'><td class~~="~~sblockLbl~~">~~<b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5opl\|5opl]]~~, [[~~5opk~~\|~~5opk]], [[5opm\|5opm]], [[5opn\|5opn~~]]</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>NT5C2, NT5B, NT5CP, PNT5 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~9606 HUMAN])</td~~><~~/tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5opo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5opo OCA], [https://pdbe.org/5opo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5opo RCSB], [https://www.ebi.ac.uk/pdbsum/5opo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5opo ProSAT]</span></td></tr>
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b~~></td><~~td class~~=~~"sblockDat"><span class~~=~~'plainlinks~~'>~~[http://en.wikipedia.org/wiki/5'-nucleotidase 5'-nucleotidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.5 3.1.3.5]~~ </~~span~~></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5opo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5opo OCA], [~~http~~://pdbe.org/5opo PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5opo RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5opo PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5opo ProSAT]</span></td></tr>	+
	</table>		</table>
	== Disease ==		== Disease ==
-	[~~[http~~://www.uniprot.org/uniprot/5NTC_HUMAN 5NTC_HUMAN]] Autosomal recessive spastic paraplegia type 45. The disease is caused by mutations affecting the gene represented in this entry.<ref>PMID:24482476</ref>	+	[https://www.uniprot.org/uniprot/5NTC_HUMAN 5NTC_HUMAN] Autosomal recessive spastic paraplegia type 45. The disease is caused by mutations affecting the gene represented in this entry.<ref>PMID:24482476</ref>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/5NTC_HUMAN 5NTC_HUMAN]] May have a critical role in the maintenance of a constant composition of intracellular purine/pyrimidine nucleotides in cooperation with other nucleotidases. Preferentially hydrolyzes inosine 5'-monophosphate (IMP) and other purine nucleotides.	+	[https://www.uniprot.org/uniprot/5NTC_HUMAN 5NTC_HUMAN] May have a critical role in the maintenance of a constant composition of intracellular purine/pyrimidine nucleotides in cooperation with other nucleotidases. Preferentially hydrolyzes inosine 5'-monophosphate (IMP) and other purine nucleotides.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~5'-nucleotidase]]~~	+	[[Category: Homo sapiens]]
-	~~[[Category: Human~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Hnizda, A]]	+	[[Category: Hnizda A]]
-	[[Category: Pachl, P]]	+	[[Category: Pachl P]]
-	[[Category: Rezacova, P]]	+	[[Category: Rezacova P]]
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Nucleotidase]]~~	+
-	~~[[Category: Relapsed leukemia~~]]	+

OCA at 06:57, 16 October 2019

OCA — Wed, 16 Oct 2019 06:57:01 GMT

←Older revision		Revision as of 06:57, 16 October 2019
Line 1:		Line 1:

	==Crystal structure of R238G cN-II mutant==		==Crystal structure of R238G cN-II mutant==
-	<StructureSection load='5opo' size='340' side='right' caption='[[5opo]], [[Resolution\|resolution]] 2.00Å' scene=''>	+	<StructureSection load='5opo' size='340' side='right'caption='[[5opo]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5opo]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OPO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5OPO FirstGlance]. <br>		<table><tr><td colspan='2'>[[5opo]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OPO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5OPO FirstGlance]. <br>
Line 29:		Line 29:
	[[Category: 5'-nucleotidase]]		[[Category: 5'-nucleotidase]]
	[[Category: Human]]		[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Hnizda, A]]		[[Category: Hnizda, A]]
	[[Category: Pachl, P]]		[[Category: Pachl, P]]

OCA at 06:18, 20 June 2018

OCA — Wed, 20 Jun 2018 06:18:54 GMT

←Older revision		Revision as of 06:18, 20 June 2018
Line 3:		Line 3:
	<StructureSection load='5opo' size='340' side='right' caption='[[5opo]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='5opo' size='340' side='right' caption='[[5opo]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5opo]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OPO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5OPO FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5opo]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OPO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5OPO FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5opl\|5opl]], [[5opk\|5opk]], [[5opm\|5opm]], [[5opn\|5opn]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5opl\|5opl]], [[5opk\|5opk]], [[5opm\|5opm]], [[5opn\|5opn]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">NT5C2, NT5B, NT5CP, PNT5 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/5'-nucleotidase 5'-nucleotidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.5 3.1.3.5] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/5'-nucleotidase 5'-nucleotidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.5 3.1.3.5] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5opo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5opo OCA], [http://pdbe.org/5opo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5opo RCSB], [http://www.ebi.ac.uk/pdbsum/5opo PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5opo ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5opo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5opo OCA], [http://pdbe.org/5opo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5opo RCSB], [http://www.ebi.ac.uk/pdbsum/5opo PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5opo ProSAT]</span></td></tr>
Line 27:		Line 28:
	</StructureSection>		</StructureSection>
	[[Category: 5'-nucleotidase]]		[[Category: 5'-nucleotidase]]
		+	[[Category: Human]]
	[[Category: Hnizda, A]]		[[Category: Hnizda, A]]
	[[Category: Pachl, P]]		[[Category: Pachl, P]]

OCA at 07:30, 14 June 2018

OCA — Thu, 14 Jun 2018 07:30:46 GMT

←Older revision		Revision as of 07:30, 14 June 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5opo is ~~ON HOLD~~ ~~until Paper~~ Publication	+	==Crystal structure of R238G cN-II mutant==
		+	<StructureSection load='5opo' size='340' side='right' caption='[[5opo]], [[Resolution\|resolution]] 2.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5opo]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OPO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5OPO FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5opl\|5opl]], [[5opk\|5opk]], [[5opm\|5opm]], [[5opn\|5opn]]</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/5'-nucleotidase 5'-nucleotidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.5 3.1.3.5] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5opo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5opo OCA], [http://pdbe.org/5opo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5opo RCSB], [http://www.ebi.ac.uk/pdbsum/5opo PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5opo ProSAT]</span></td></tr>
		+	</table>
		+	== Disease ==
		+	[[http://www.uniprot.org/uniprot/5NTC_HUMAN 5NTC_HUMAN]] Autosomal recessive spastic paraplegia type 45. The disease is caused by mutations affecting the gene represented in this entry.<ref>PMID:24482476</ref>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/5NTC_HUMAN 5NTC_HUMAN]] May have a critical role in the maintenance of a constant composition of intracellular purine/pyrimidine nucleotides in cooperation with other nucleotidases. Preferentially hydrolyzes inosine 5'-monophosphate (IMP) and other purine nucleotides.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Activating mutations in NT5C2, a gene encoding cytosolic purine 5'-nucleotidase (cN-II), confer chemoresistance in relapsed acute lymphoblastic leukemia. Here we show that all mutants became independent of allosteric effects of ATP and thus constitutively active. Structural mapping of mutations described in patients demonstrates that 90% of leukemia-specific allelles directly affect two regulatory hotspots within the cN-II molecule-the helix A region: residues 355-365, and the intersubunit interface: helix B (232-242) and flexible interhelical loop L (400-418). Furthermore, analysis of hetero-oligomeric complexes combining wild-type (WT) and mutant subunits showed that the activation is transmitted from the mutated to the WT subunit. This intersubunit interaction forms structural basis of hyperactive NT5C2 in drug-resistant leukemia in which heterozygous NT5C2 mutation gave rise to hetero-tetramer mutant and WT proteins. This enabled us to define criteria to aid the prediction of NT5C2 drug resistance mutations in leukemia.

-	~~Authors: Hnizda~~, A., Pachl, P., ~~Rezacova~~, P.	+	Relapsed acute lymphoblastic leukemia-specific mutations in NT5C2 cluster into hotspots driving intersubunit stimulation.,Hnizda A, Fabry M, Moriyama T, Pachl P, Kugler M, Brinsa V, Ascher DB, Carroll WL, Novak P, Zaliova M, Trka J, Rezacova P, Yang JJ, Veverka V Leukemia. 2018 Jun;32(6):1393-1403. doi: 10.1038/s41375-018-0073-5. Epub 2018 Feb, 25. PMID:29535428<ref>PMID:29535428</ref>

-	~~Description: Crystal structure~~ of ~~R238G cN~~-~~II mutant~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 5opo" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: 5'-nucleotidase]]
	[[Category: Hnizda, A]]		[[Category: Hnizda, A]]
	[[Category: Pachl, P]]		[[Category: Pachl, P]]
	[[Category: Rezacova, P]]		[[Category: Rezacova, P]]
		+	[[Category: Hydrolase]]
		+	[[Category: Nucleotidase]]
		+	[[Category: Relapsed leukemia]]

OCA: Protected "5opo" [edit=sysop:move=sysop]

OCA — Wed, 27 Sep 2017 12:41:18 GMT

Protected "5opo" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:41, 27 September 2017

OCA: New page: '''Unreleased structure''' The entry 5opo is ON HOLD until Paper Publication Authors: Hnizda, A., Pachl, P., Rezacova, P. Description: Crystal structure of R238G cN-II mutant [[Categor...

OCA — Wed, 27 Sep 2017 12:41:17 GMT

New page: '''Unreleased structure''' The entry 5opo is ON HOLD until Paper Publication Authors: Hnizda, A., Pachl, P., Rezacova, P. Description: Crystal structure of R238G cN-II mutant [[Categor...

New page

'''Unreleased structure'''

The entry 5opo is ON HOLD until Paper Publication

Authors: Hnizda, A., Pachl, P., Rezacova, P.

Description: Crystal structure of R238G cN-II mutant
[[Category: Unreleased Structures]]
[[Category: Hnizda, A]]
[[Category: Pachl, P]]
[[Category: Rezacova, P]]