5oq9 - Revision history

OCA at 09:52, 15 November 2023

OCA — Wed, 15 Nov 2023 09:52:52 GMT

←Older revision		Revision as of 09:52, 15 November 2023
Line 3:		Line 3:
	<StructureSection load='5oq9' size='340' side='right'caption='[[5oq9]], [[Resolution\|resolution]] 1.51Å' scene=''>		<StructureSection load='5oq9' size='340' side='right'caption='[[5oq9]], [[Resolution\|resolution]] 1.51Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5oq9]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Lobhe Lobhe~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OQ9 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5OQ9 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5oq9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Lobophyllia_hemprichii Lobophyllia hemprichii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OQ9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5OQ9 FirstGlance]. <br>
-	</td></tr><tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non~~-~~Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=VYA:'>VYA</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.51Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5oq9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5oq9 OCA], [~~http~~://pdbe.org/5oq9 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5oq9 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5oq9 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5oq9 ProSAT]</span></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=VYA:2-[(4~{Z})-2-[(1~{S})-1-azanyl-3-methyl-butyl]-4-[(4-hydroxyphenyl)methylidene]-5-oxidanylidene-imidazol-1-yl]ethanoic+acid'>VYA</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5oq9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5oq9 OCA], [https://pdbe.org/5oq9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5oq9 RCSB], [https://www.ebi.ac.uk/pdbsum/5oq9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5oq9 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q5S6Z9_LOBHE Q5S6Z9_LOBHE]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 24:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Lobhe~~]]	+	[[Category: Lobophyllia hemprichii]]
-	[[Category: Cordon-Preciado, V]]	+	[[Category: Cordon-Preciado V]]
-	[[Category: Hutchison~~, C D.M~~]]	+	[[Category: Hutchison CDM]]
-	[[Category: Morgan~~, R M.L~~]]	+	[[Category: Morgan RML]]
-	[[Category: Thor~~, J J.van]]~~	+	[[Category: Van Thor JJ]]
-	~~[[Category: Fluorescent protein]]~~	+
-	~~[[Category: Xfel photoswitching~~]]	+

OCA at 06:57, 16 October 2019

OCA — Wed, 16 Oct 2019 06:57:11 GMT

←Older revision		Revision as of 06:57, 16 October 2019
Line 1:		Line 1:

	==XFEL structure of the off state of a reversibly photoswitching fluorescent protein determined using the droplet on demand injection method==		==XFEL structure of the off state of a reversibly photoswitching fluorescent protein determined using the droplet on demand injection method==
-	<StructureSection load='5oq9' size='340' side='right' caption='[[5oq9]], [[Resolution\|resolution]] 1.51Å' scene=''>	+	<StructureSection load='5oq9' size='340' side='right'caption='[[5oq9]], [[Resolution\|resolution]] 1.51Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5oq9]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Lobhe Lobhe]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OQ9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5OQ9 FirstGlance]. <br>		<table><tr><td colspan='2'>[[5oq9]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Lobhe Lobhe]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OQ9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5OQ9 FirstGlance]. <br>
Line 20:		Line 20:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Lobhe]]		[[Category: Lobhe]]
	[[Category: Cordon-Preciado, V]]		[[Category: Cordon-Preciado, V]]

OCA at 08:52, 14 November 2018

OCA — Wed, 14 Nov 2018 08:52:14 GMT

←Older revision		Revision as of 08:52, 14 November 2018
Line 3:		Line 3:
	<StructureSection load='5oq9' size='340' side='right' caption='[[5oq9]], [[Resolution\|resolution]] 1.51Å' scene=''>		<StructureSection load='5oq9' size='340' side='right' caption='[[5oq9]], [[Resolution\|resolution]] 1.51Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5oq9]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OQ9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5OQ9 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5oq9]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Lobhe Lobhe]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OQ9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5OQ9 FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=VYA:'>VYA</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=VYA:'>VYA</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5oq9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5oq9 OCA], [http://pdbe.org/5oq9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5oq9 RCSB], [http://www.ebi.ac.uk/pdbsum/5oq9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5oq9 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5oq9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5oq9 OCA], [http://pdbe.org/5oq9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5oq9 RCSB], [http://www.ebi.ac.uk/pdbsum/5oq9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5oq9 ProSAT]</span></td></tr>
Line 20:		Line 20:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Lobhe]]
	[[Category: Cordon-Preciado, V]]		[[Category: Cordon-Preciado, V]]
	[[Category: Hutchison, C D.M]]		[[Category: Hutchison, C D.M]]

OCA at 07:12, 6 December 2017

OCA — Wed, 06 Dec 2017 07:12:31 GMT

←Older revision		Revision as of 07:12, 6 December 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5oq9 is ~~ON HOLD~~	+	==XFEL structure of the off state of a reversibly photoswitching fluorescent protein determined using the droplet on demand injection method==
		+	<StructureSection load='5oq9' size='340' side='right' caption='[[5oq9]], [[Resolution\|resolution]] 1.51Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5oq9]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OQ9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5OQ9 FirstGlance]. <br>
		+	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=VYA:'>VYA</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5oq9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5oq9 OCA], [http://pdbe.org/5oq9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5oq9 RCSB], [http://www.ebi.ac.uk/pdbsum/5oq9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5oq9 ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The photochromic fluorescent protein Skylan-NS (Nonlinear Structured illumination variant mEos3.1H62L) is a reversibly photoswitchable fluorescent protein which has an unilluminated/ground state with an anionic and cis chromophore conformation and high fluorescence quantum yield. Photo-conversion with illumination at 515 nm generates a meta-stable intermediate with neutral trans-chromophore structure that has a 4 h lifetime. We present X-ray crystal structures of the cis (on) state at 1.9 Angstrom resolution and the trans (off) state at a limiting resolution of 1.55 Angstrom from serial femtosecond crystallography experiments conducted at SPring-8 Angstrom Compact Free Electron Laser (SACLA) at 7.0 keV and 10.5 keV, and at Linac Coherent Light Source (LCLS) at 9.5 keV. We present a comparison of the data reduction and structure determination statistics for the two facilities which differ in flux, beam characteristics and detector technologies. Furthermore, a comparison of droplet on demand, grease injection and Gas Dynamic Virtual Nozzle (GDVN) injection shows no significant differences in limiting resolution. The photoconversion of the on- to the off-state includes both internal and surface exposed protein structural changes, occurring in regions that lack crystal contacts in the orthorhombic crystal form.

-	~~Authors: Hutchison, C.D.M~~., Cordon-Preciado, V., Morgan, R~~.M.L.~~, van Thor, J.J.	+	X-ray Free Electron Laser Determination of Crystal Structures of Dark and Light States of a Reversibly Photoswitching Fluorescent Protein at Room Temperature.,Hutchison CDM, Cordon-Preciado V, Morgan RML, Nakane T, Ferreira J, Dorlhiac G, Sanchez-Gonzalez A, Johnson AS, Fitzpatrick A, Fare C, Marangos JP, Yoon CH, Hunter MS, DePonte DP, Boutet S, Owada S, Tanaka R, Tono K, Iwata S, van Thor JJ Int J Mol Sci. 2017 Sep 7;18(9). pii: ijms18091918. doi: 10.3390/ijms18091918. PMID:28880248<ref>PMID:28880248</ref>

-	~~Description: XFEL structure~~ of the ~~off state~~ of ~~a reversibly photoswitching fluorescent protein determined using the droplet on demand injection method~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category: Unreleased Structures]]~~	+	</div>
-	~~[[Category~~: ~~Van Thor, J.J]]~~	+	<div class="pdbe-citations 5oq9" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Cordon-Preciado, V]]		[[Category: Cordon-Preciado, V]]
-	[[Category: Morgan, R.M.L]]	+	[[Category: Hutchison, C D.M]]
-	[[Category: ~~Hutchison~~, C.~~D.M~~]]	+	[[Category: Morgan, R M.L]]
		+	[[Category: Thor, J J.van]]
		+	[[Category: Fluorescent protein]]
		+	[[Category: Xfel photoswitching]]

OCA: Protected "5oq9" [edit=sysop:move=sysop]

OCA — Wed, 27 Sep 2017 12:42:54 GMT

Protected "5oq9" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:42, 27 September 2017

OCA: New page: '''Unreleased structure''' The entry 5oq9 is ON HOLD Authors: Hutchison, C.D.M., Cordon-Preciado, V., Morgan, R.M.L., van Thor, J.J. Description: XFEL structure of the off state of a r...

OCA — Wed, 27 Sep 2017 12:42:53 GMT

New page: '''Unreleased structure''' The entry 5oq9 is ON HOLD Authors: Hutchison, C.D.M., Cordon-Preciado, V., Morgan, R.M.L., van Thor, J.J. Description: XFEL structure of the off state of a r...

New page

'''Unreleased structure'''

The entry 5oq9 is ON HOLD

Authors: Hutchison, C.D.M., Cordon-Preciado, V., Morgan, R.M.L., van Thor, J.J.

Description: XFEL structure of the off state of a reversibly photoswitching fluorescent protein determined using the droplet on demand injection method
[[Category: Unreleased Structures]]
[[Category: Van Thor, J.J]]
[[Category: Cordon-Preciado, V]]
[[Category: Morgan, R.M.L]]
[[Category: Hutchison, C.D.M]]