5osa - Revision history

OCA at 12:58, 20 December 2023

OCA — Wed, 20 Dec 2023 12:58:34 GMT

←Older revision		Revision as of 12:58, 20 December 2023
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	<StructureSection load='5osa' size='340' side='right'caption='[[5osa]], [[Resolution\|resolution]] 2.75Å' scene=''>		<StructureSection load='5osa' size='340' side='right'caption='[[5osa]], [[Resolution\|resolution]] 2.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5osa]] is a 5 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Lk3_transgenic_mice Lk3 transgenic mice~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OSA OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5OSA FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5osa]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Gloeobacter_violaceus_PCC_7421 Gloeobacter violaceus PCC 7421] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OSA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5OSA FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=D12:DODECANE'>D12</scene>, <scene name='pdbligand=Y01:CHOLESTEROL+HEMISUCCINATE'>Y01</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.7500024Å</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Gabra1, Gabra-1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=D12:DODECANE'>D12</scene>, <scene name='pdbligand=Y01:CHOLESTEROL+HEMISUCCINATE'>Y01</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5osa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5osa OCA], [~~http~~://pdbe.org/5osa PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5osa RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5osa PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5osa ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5osa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5osa OCA], [https://pdbe.org/5osa PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5osa RCSB], [https://www.ebi.ac.uk/pdbsum/5osa PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5osa ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/GLIC_GLOVI GLIC_GLOVI]] Cationic channel with similar permeabilities for Na(+) and K(+), that is activated by an increase of the proton concentration on the extracellular side. Displays no permeability for chloride ions. Shows slow kinetics of activation, no desensitization and a single channel conductance of 8 pS. Might contribute to adaptation to external pH change.<ref>PMID:17167423</ref>	+	[https://www.uniprot.org/uniprot/GBRA1_MOUSE GBRA1_MOUSE] Ligand-gated chloride channel which is a component of the heteropentameric receptor for GABA, the major inhibitory neurotransmitter in the brain (PubMed:27129275). Plays an important role in the formation of functional inhibitory GABAergic synapses in addition to mediating synaptic inhibition as a GABA-gated ion channel (PubMed:27129275). The gamma2 subunit is necessary but not sufficient for a rapid formation of active synaptic contacts and the synaptogenic effect of this subunit is influenced by the type of alpha and beta subunits present in the receptor pentamer (PubMed:27129275). The alpha1/beta2/gamma2 receptor and the alpha1/beta3/gamma2 receptor exhibit synaptogenic activity (PubMed:27129275). GABRA1-mediated plasticity in the orbitofrontal cortex regulates context-dependent action selection (PubMed:25348603). Functions also as histamine receptor and mediates cellular responses to histamine (By similarity).[UniProtKB:P62813]<ref>PMID:25348603</ref> <ref>PMID:27129275</ref> [https://www.uniprot.org/uniprot/GLIC_GLOVI GLIC_GLOVI] Cationic channel with similar permeabilities for Na(+) and K(+), that is activated by an increase of the proton concentration on the extracellular side. Displays no permeability for chloride ions. Shows slow kinetics of activation, no desensitization and a single channel conductance of 8 pS. Might contribute to adaptation to external pH change.<ref>PMID:17167423</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 5osa" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5osa" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Ion channels 3D structures\|Ion channels 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Gloeobacter violaceus PCC 7421]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Lk3 transgenic mice~~]]	+	[[Category: Mus musculus]]
-	[[Category: Gold~~, M G~~]]	+	[[Category: Gold MG]]
-	[[Category: Laverty~~, D C~~]]	+	[[Category: Laverty DC]]
-	[[Category: Smart~~, T G]]~~	+	[[Category: Smart TG]]
-	~~[[Category: Gabaa-receptor ion channel ion transport extracellular ligand gated ion channel]]~~	+
-	~~[[Category: Transport protein~~]]	+

OCA at 09:26, 30 October 2019

OCA — Wed, 30 Oct 2019 09:26:08 GMT

←Older revision		Revision as of 09:26, 30 October 2019
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	==GLIC-GABAAR alpha1 chimera crystallized at pH4.6==		==GLIC-GABAAR alpha1 chimera crystallized at pH4.6==
-	<StructureSection load='5osa' size='340' side='right' caption='[[5osa]], [[Resolution\|resolution]] 2.75Å' scene=''>	+	<StructureSection load='5osa' size='340' side='right'caption='[[5osa]], [[Resolution\|resolution]] 2.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5osa]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OSA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5OSA FirstGlance]. <br>		<table><tr><td colspan='2'>[[5osa]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OSA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5OSA FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=D12:DODECANE'>D12</scene>, <scene name='pdbligand=Y01:CHOLESTEROL+HEMISUCCINATE'>Y01</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=D12:DODECANE'>D12</scene>, <scene name='pdbligand=Y01:CHOLESTEROL+HEMISUCCINATE'>Y01</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">~~N326_04548~~ ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Gabra1, Gabra-1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5osa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5osa OCA], [http://pdbe.org/5osa PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5osa RCSB], [http://www.ebi.ac.uk/pdbsum/5osa PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5osa ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5osa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5osa OCA], [http://pdbe.org/5osa PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5osa RCSB], [http://www.ebi.ac.uk/pdbsum/5osa PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5osa ProSAT]</span></td></tr>
	</table>		</table>
Line 23:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Lk3 transgenic mice]]		[[Category: Lk3 transgenic mice]]
	[[Category: Gold, M G]]		[[Category: Gold, M G]]

OCA at 10:14, 15 November 2017

OCA — Wed, 15 Nov 2017 10:14:06 GMT

←Older revision		Revision as of 10:14, 15 November 2017
Line 3:		Line 3:
	<StructureSection load='5osa' size='340' side='right' caption='[[5osa]], [[Resolution\|resolution]] 2.75Å' scene=''>		<StructureSection load='5osa' size='340' side='right' caption='[[5osa]], [[Resolution\|resolution]] 2.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5osa]] is a 5 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OSA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5OSA FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5osa]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OSA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5OSA FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=D12:DODECANE'>D12</scene>, <scene name='pdbligand=Y01:CHOLESTEROL+HEMISUCCINATE'>Y01</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=D12:DODECANE'>D12</scene>, <scene name='pdbligand=Y01:CHOLESTEROL+HEMISUCCINATE'>Y01</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">N326_04548 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5osa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5osa OCA], [http://pdbe.org/5osa PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5osa RCSB], [http://www.ebi.ac.uk/pdbsum/5osa PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5osa ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5osa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5osa OCA], [http://pdbe.org/5osa PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5osa RCSB], [http://www.ebi.ac.uk/pdbsum/5osa PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5osa ProSAT]</span></td></tr>
	</table>		</table>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Lk3 transgenic mice]]
	[[Category: Gold, M G]]		[[Category: Gold, M G]]
	[[Category: Laverty, D C]]		[[Category: Laverty, D C]]

OCA at 06:56, 11 October 2017

OCA — Wed, 11 Oct 2017 06:56:08 GMT

←Older revision		Revision as of 06:56, 11 October 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5osa is ~~ON HOLD~~ ~~until Paper~~ Publication	+	==GLIC-GABAAR alpha1 chimera crystallized at pH4.6==
		+	<StructureSection load='5osa' size='340' side='right' caption='[[5osa]], [[Resolution\|resolution]] 2.75Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5osa]] is a 5 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OSA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5OSA FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=D12:DODECANE'>D12</scene>, <scene name='pdbligand=Y01:CHOLESTEROL+HEMISUCCINATE'>Y01</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5osa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5osa OCA], [http://pdbe.org/5osa PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5osa RCSB], [http://www.ebi.ac.uk/pdbsum/5osa PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5osa ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/GLIC_GLOVI GLIC_GLOVI]] Cationic channel with similar permeabilities for Na(+) and K(+), that is activated by an increase of the proton concentration on the extracellular side. Displays no permeability for chloride ions. Shows slow kinetics of activation, no desensitization and a single channel conductance of 8 pS. Might contribute to adaptation to external pH change.<ref>PMID:17167423</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	gamma-Aminobutyric acid receptors (GABAARs) are vital for controlling excitability in the brain. This is emphasized by the numerous neuropsychiatric disorders that result from receptor dysfunction. A critical component of most native GABAARs is the alpha subunit. Its transmembrane domain is the target for many modulators, including endogenous brain neurosteroids that impact anxiety, stress and depression, and for therapeutic drugs, such as general anesthetics. Understanding the basis for the modulation of GABAAR function requires high-resolution structures. Here we present the first atomic structures of a GABAAR chimera at 2.8-A resolution, including those bound with potentiating and inhibitory neurosteroids. These structures define new allosteric binding sites for these modulators that are associated with the alpha-subunit transmembrane domain. Our findings will enable the exploitation of neurosteroids for therapeutic drug design to regulate GABAARs in neurological disorders.

-	~~Authors: Laverty~~, D~~.C.~~, Gold, M~~.G.~~, Smart~~, T~~.G.	+	Crystal structures of a GABAA-receptor chimera reveal new endogenous neurosteroid-binding sites.,Laverty D, Thomas P, Field M, Andersen OJ, Gold MG, Biggin PC, Gielen M, Smart TG Nat Struct Mol Biol. 2017 Oct 2. doi: 10.1038/nsmb.3477. PMID:28967882<ref>PMID:28967882</ref>

-	~~Description: GLIC-GABAAR alpha1 chimera crystallized at pH4~~.6	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category~~: ~~Unreleased Structures]]~~	+	</div>
-	[[Category: Gold, M.G]]	+	<div class="pdbe-citations 5osa" style="background-color:#fffaf0;"></div>
-	[[Category: Laverty, D.C]]	+	== References ==
-	[[Category: Smart, T.G]]	+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Gold, M G]]
		+	[[Category: Laverty, D C]]
		+	[[Category: Smart, T G]]
		+	[[Category: Gabaa-receptor ion channel ion transport extracellular ligand gated ion channel]]
		+	[[Category: Transport protein]]

OCA: Protected "5osa" [edit=sysop:move=sysop]

OCA — Wed, 27 Sep 2017 12:47:31 GMT

Protected "5osa" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:47, 27 September 2017

OCA: New page: '''Unreleased structure''' The entry 5osa is ON HOLD until Paper Publication Authors: Laverty, D.C., Gold, M.G., Smart, T.G. Description: GLIC-GABAAR alpha1 chimera crystallized at pH4...

OCA — Wed, 27 Sep 2017 12:47:30 GMT

New page: '''Unreleased structure''' The entry 5osa is ON HOLD until Paper Publication Authors: Laverty, D.C., Gold, M.G., Smart, T.G. Description: GLIC-GABAAR alpha1 chimera crystallized at pH4...

New page

'''Unreleased structure'''

The entry 5osa is ON HOLD until Paper Publication

Authors: Laverty, D.C., Gold, M.G., Smart, T.G.

Description: GLIC-GABAAR alpha1 chimera crystallized at pH4.6
[[Category: Unreleased Structures]]
[[Category: Gold, M.G]]
[[Category: Laverty, D.C]]
[[Category: Smart, T.G]]