5ovu - Revision history

OCA at 01:26, 28 December 2023

OCA — Thu, 28 Dec 2023 01:26:00 GMT

←Older revision		Revision as of 01:26, 28 December 2023
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	==Cupriavidus metallidurans BPH==		==Cupriavidus metallidurans BPH==
-	<StructureSection load='5ovu' size='340' side='right' caption='[[5ovu]], [[Resolution\|resolution]] 2.10Å' scene=''>	+	<StructureSection load='5ovu' size='340' side='right'caption='[[5ovu]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5ovu]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Atcc_43123 Atcc 43123~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OVU OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5OVU FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5ovu]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Cupriavidus_metallidurans Cupriavidus metallidurans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OVU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5OVU FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat"~~><scene name~~=~~'pdbligand=MLI:MALONATE+ION'>MLI</scene~~></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.1Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~Rmet_1198 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=~~5&id=119219 ATCC 43123])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MLI:MALONATE+ION'>MLI</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5ovu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ovu OCA], [~~http~~://pdbe.org/5ovu PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5ovu RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5ovu PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5ovu ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ovu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ovu OCA], [https://pdbe.org/5ovu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ovu RCSB], [https://www.ebi.ac.uk/pdbsum/5ovu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ovu ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q1LP42_CUPMC Q1LP42_CUPMC]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Atcc 43123~~]]	+	[[Category: Cupriavidus metallidurans]]
-	[[Category: ~~Albrecht, R~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Fuchs, A C.D~~]]	+	[[Category: Albrecht R]]
-	[[Category: ~~Hartmann, M D~~]]	+	[[Category: Fuchs ACD]]
-	[[Category: ~~Martin, J~~]]	+	[[Category: Hartmann MD]]
-	[[Category: ~~Beta-proteobacteria proteasome homologue]]~~	+	[[Category: Martin J]]
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Protein degradation~~]]	+

OCA at 07:30, 13 December 2017

OCA — Wed, 13 Dec 2017 07:30:55 GMT

←Older revision		Revision as of 07:30, 13 December 2017
Line 3:		Line 3:
	<StructureSection load='5ovu' size='340' side='right' caption='[[5ovu]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='5ovu' size='340' side='right' caption='[[5ovu]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5ovu]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OVU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5OVU FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5ovu]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_43123 Atcc 43123]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OVU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5OVU FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MLI:MALONATE+ION'>MLI</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MLI:MALONATE+ION'>MLI</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Rmet_1198 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=119219 ATCC 43123])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ovu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ovu OCA], [http://pdbe.org/5ovu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ovu RCSB], [http://www.ebi.ac.uk/pdbsum/5ovu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ovu ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ovu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ovu OCA], [http://pdbe.org/5ovu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ovu RCSB], [http://www.ebi.ac.uk/pdbsum/5ovu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ovu ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Eukaryotic and archaeal proteasomes are paradigms for self-compartmentalizing proteases. To a large extent, their function requires the interplay with hexameric ATPases associated with diverse cellular activities (AAA+) that act as substrate unfoldases. Bacteria have various types of self-compartmentalizing proteases; in addition to the proteasome itself, these include the proteasome homolog HslV, which functions together with the AAA+ ATPase HslU; the ClpP protease with its partner AAA+ ATPase ClpX; and Anbu, a recently characterized ancestral proteasome variant. Previous bioinformatic analysis has revealed a novel bacterial member of the proteasome family, BPH (Beta-proteobacteria proteasome homolog). Using cluster analysis, we here affirmed that BPH evolutionarily descends from HslV. Crystal structures of the Thiobacillus denitrificans and Cupriavidus metallidurans BPHs disclosed a homo-oligomeric double-ring architecture, in which the active sites face the interior of the cylinder. Using small-angle X-ray scattering (SAXS) and electron microscopy averaging, we found that BPH forms tetradecamers in solution, unlike the dodecamers seen in HslV. While the highly acidic inner surface of BPH was in striking contrast to the cavity characteristics of the proteasome and HslV, a classical proteasomal reaction mechanism could be inferred from the covalent binding of the proteasome-specific inhibitor epoxomicin to BPH. A ligand-bound structure implied that the elongated BPH inner pore loop may be involved in substrate recognition. The apparent lack of a partner unfoldase and other unique features, such as Ser replacing Thr as catalytic residue in certain BPH subfamilies, suggest a proteolytic function for BPH distinct from those of known bacterial self-compartmentalizing proteases. .
		+
		+	Structural characterization of the bacterial proteasome homolog BPH reveals a tetradecameric double-ring complex with unique inner cavity properties.,Fuchs ACD, Maldoner L, Hipp K, Hartmann MD, Martin J J Biol Chem. 2017 Nov 28. pii: M117.815258. doi: 10.1074/jbc.M117.815258. PMID:29183996<ref>PMID:29183996</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5ovu" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Atcc 43123]]
	[[Category: Albrecht, R]]		[[Category: Albrecht, R]]
	[[Category: Fuchs, A C.D]]		[[Category: Fuchs, A C.D]]

OCA at 07:12, 6 December 2017

OCA — Wed, 06 Dec 2017 07:12:44 GMT

←Older revision		Revision as of 07:12, 6 December 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5ovu is ~~ON HOLD until Paper Publication~~	+	==Cupriavidus metallidurans BPH==
-		+	<StructureSection load='5ovu' size='340' side='right' caption='[[5ovu]], [[Resolution\|resolution]] 2.10Å' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5ovu]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OVU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5OVU FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MLI:MALONATE+ION'>MLI</scene></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ovu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ovu OCA], [http://pdbe.org/5ovu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ovu RCSB], [http://www.ebi.ac.uk/pdbsum/5ovu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ovu ProSAT]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Albrecht, R]]
		+	[[Category: Fuchs, A C.D]]
		+	[[Category: Hartmann, M D]]
		+	[[Category: Martin, J]]
		+	[[Category: Beta-proteobacteria proteasome homologue]]
		+	[[Category: Hydrolase]]
		+	[[Category: Protein degradation]]

OCA: Protected "5ovu" [edit=sysop:move=sysop]

OCA — Wed, 27 Sep 2017 12:54:26 GMT

Protected "5ovu" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:54, 27 September 2017

OCA: New page: '''Unreleased structure''' The entry 5ovu is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

OCA — Wed, 27 Sep 2017 12:54:25 GMT

New page: '''Unreleased structure''' The entry 5ovu is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 5ovu is ON HOLD until Paper Publication

Authors:

Description:
[[Category: Unreleased Structures]]