5oxw - Revision history

OCA at 01:28, 28 December 2023

OCA — Thu, 28 Dec 2023 01:28:01 GMT

←Older revision		Revision as of 01:28, 28 December 2023
Line 1:		Line 1:

	==Structure of NeqN from Nanoarchaeum equitans==		==Structure of NeqN from Nanoarchaeum equitans==
-	<StructureSection load='5oxw' size='340' side='right' caption='[[5oxw]], [[Resolution\|resolution]] 2.61Å' scene=''>	+	<StructureSection load='5oxw' size='340' side='right'caption='[[5oxw]], [[Resolution\|resolution]] 2.61Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5oxw]] is a 8 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"nanoarchaeum_equitans"_huber_et_al._2002 "nanoarchaeum~~ equitans~~" huber et al. 2002~~] and [~~http~~://en.wikipedia.org/wiki/~~Microarchaeum_sp._kin4~~-~~m Microarchaeum sp. kin4~~-m]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OXW OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5OXW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5oxw]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Nanoarchaeum_equitans Nanoarchaeum equitans] and [https://en.wikipedia.org/wiki/Nanoarchaeum_equitans_Kin4-M Nanoarchaeum equitans Kin4-M]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OXW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5OXW FirstGlance]. <br>
-	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~NEQ068 (~~[~~http://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=228908 Microarchaeum sp. Kin4-M])~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.61Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5oxw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5oxw OCA], [~~http~~://pdbe.org/5oxw PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5oxw RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5oxw PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5oxw ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5oxw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5oxw OCA], [https://pdbe.org/5oxw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5oxw RCSB], [https://www.ebi.ac.uk/pdbsum/5oxw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5oxw ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q74N74_NANEQ Q74N74_NANEQ]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 20:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Nanoarchaeum equitans ~~huber et al. 2002~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Microarchaeum sp. kin4~~-m]]	+	[[Category: Nanoarchaeum equitans]]
-	[[Category: Aparicio, D]]	+	[[Category: Nanoarchaeum equitans Kin4-M]]
-	[[Category: Fita, I]]	+	[[Category: Aparicio D]]
-	[[Category: Perez-Luque, R]]	+	[[Category: Fita I]]
-	[[Category: Ribo, M]]	+	[[Category: Perez-Luque R]]
-	~~[[Category: Intein complex]]~~	+	[[Category: Ribo M]]
-	~~[[Category: Splicing~~]]	+

OCA at 08:26, 17 October 2018

OCA — Wed, 17 Oct 2018 08:26:46 GMT

←Older revision		Revision as of 08:26, 17 October 2018
Line 1:		Line 1:

-	==~~Intein~~==	+	==Structure of NeqN from Nanoarchaeum equitans==
	<StructureSection load='5oxw' size='340' side='right' caption='[[5oxw]], [[Resolution\|resolution]] 2.61Å' scene=''>		<StructureSection load='5oxw' size='340' side='right' caption='[[5oxw]], [[Resolution\|resolution]] 2.61Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5oxw]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OXW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5OXW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5oxw]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/"nanoarchaeum_equitans"_huber_et_al._2002 "nanoarchaeum equitans" huber et al. 2002] and [http://en.wikipedia.org/wiki/Microarchaeum_sp._kin4-m Microarchaeum sp. kin4-m]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OXW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5OXW FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5oxw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5oxw OCA], [http://pdbe.org/5oxw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5oxw RCSB], [http://www.ebi.ac.uk/pdbsum/5oxw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5oxw ProSAT]</span></td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">NEQ068 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=228908 Microarchaeum sp. Kin4-M])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5oxw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5oxw OCA], [http://pdbe.org/5oxw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5oxw RCSB], [http://www.ebi.ac.uk/pdbsum/5oxw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5oxw ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Split inteins are expressed as two separated subunits (N-intein and C-intein) fused to the corresponding exteins. The specific association of both intein subunits precedes protein splicing, which results in excision of the intein subunits and in ligation, by a peptide bond, of the concomitant exteins. Catalytically active intein precursors are typically too reactive for crystallization or even isolation. Neq pol is the trans-intein of the B-type DNA polymerase I split gene from hyperthermophile Nanoarchaeum equitans. We have determined the crystal structures of both the isolated NeqN and the complex of NeqN and NeqC subunits carrying the wild-type sequences, including the essential catalytic residues Ser1 and Thr+1, in addition to seven and three residues of the N- and C-exteins, respectively. These structures provide detailed information on the unique oxyester chemistry of the splicing mechanism of Neq pol and of the extensive rearrangements that occur in NeqN during the association step.
		+
		+	Structural Insights into Subunits Assembly and the Oxyester Splicing Mechanism of Neq pol Split Intein.,Gordo V, Aparicio D, Perez-Luque R, Benito A, Vilanova M, Uson I, Fita I, Ribo M Cell Chem Biol. 2018 Jul 19;25(7):871-879.e2. doi:, 10.1016/j.chembiol.2018.04.008. Epub 2018 May 10. PMID:29754955<ref>PMID:29754955</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5oxw" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Nanoarchaeum equitans huber et al. 2002]]
		+	[[Category: Microarchaeum sp. kin4-m]]
	[[Category: Aparicio, D]]		[[Category: Aparicio, D]]
	[[Category: Fita, I]]		[[Category: Fita, I]]

OCA at 07:47, 10 October 2018

OCA — Wed, 10 Oct 2018 07:47:05 GMT

←Older revision		Revision as of 07:47, 10 October 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5oxw ~~is ON HOLD until Paper Publication~~	+	==Intein==
-		+	<StructureSection load='5oxw' size='340' side='right' caption='[[5oxw]], [[Resolution\|resolution]] 2.61Å' scene=''>
-	~~Authors~~: ~~Aparicio, D~~.~~, Perez~~-~~Luque, R~~.~~, Ribo, M~~.~~, Fita, I~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5oxw]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OXW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5OXW FirstGlance]. <br>
-	~~Description~~: ~~Intein~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5oxw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5oxw OCA], [http://pdbe.org/5oxw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5oxw RCSB], [http://www.ebi.ac.uk/pdbsum/5oxw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5oxw ProSAT]</span></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[[~~Category~~: ~~Perez-Luque~~, R]]	+	__TOC__
		+	</StructureSection>
	[[Category: Aparicio, D]]		[[Category: Aparicio, D]]
	[[Category: Fita, I]]		[[Category: Fita, I]]
		+	[[Category: Perez-Luque, R]]
	[[Category: Ribo, M]]		[[Category: Ribo, M]]
		+	[[Category: Intein complex]]
		+	[[Category: Splicing]]

OCA at 05:30, 25 October 2017

OCA — Wed, 25 Oct 2017 05:30:47 GMT

←Older revision		Revision as of 05:30, 25 October 2017
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5oxw is ON HOLD	+	The entry 5oxw is ON HOLD until Paper Publication

	Authors: Aparicio, D., Perez-Luque, R., Ribo, M., Fita, I.		Authors: Aparicio, D., Perez-Luque, R., Ribo, M., Fita, I.

OCA: Protected "5oxw" [edit=sysop:move=sysop]

OCA — Wed, 27 Sep 2017 12:59:00 GMT

Protected "5oxw" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:59, 27 September 2017

OCA: New page: '''Unreleased structure''' The entry 5oxw is ON HOLD Authors: Aparicio, D., Perez-Luque, R., Ribo, M., Fita, I. Description: Intein Category: Unreleased Structures [[Category: Pere...

OCA — Wed, 27 Sep 2017 12:58:59 GMT

New page: '''Unreleased structure''' The entry 5oxw is ON HOLD Authors: Aparicio, D., Perez-Luque, R., Ribo, M., Fita, I. Description: Intein Category: Unreleased Structures [[Category: Pere...

New page

'''Unreleased structure'''

The entry 5oxw is ON HOLD

Authors: Aparicio, D., Perez-Luque, R., Ribo, M., Fita, I.

Description: Intein
[[Category: Unreleased Structures]]
[[Category: Perez-Luque, R]]
[[Category: Aparicio, D]]
[[Category: Fita, I]]
[[Category: Ribo, M]]