5suq - Revision history

OCA at 12:48, 4 October 2023

OCA — Wed, 04 Oct 2023 12:48:09 GMT

←Older revision		Revision as of 12:48, 4 October 2023
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-	==~~Structure~~ of ~~mRNA export factors~~==	+	==Crystal structure of the THO-Sub2 complex==
	<StructureSection load='5suq' size='340' side='right'caption='[[5suq]], [[Resolution\|resolution]] 6.00Å' scene=''>		<StructureSection load='5suq' size='340' side='right'caption='[[5suq]], [[Resolution\|resolution]] 6.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5suq]] is a 6 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Atcc_18824 Atcc 18824~~] and [~~http~~://en.wikipedia.org/wiki/~~Baker's_yeast Baker's yeast~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5SUQ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5SUQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5suq]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] and [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5SUQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5SUQ FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=KEG:12~~-~~TUNGSTOPHOSPHATE'>KEG</scene></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 6Å</td></tr>
-	~~<tr id='NonStdRes'><td class="sblockLbl"><b>~~[[~~Non-Standard_Residue~~\|~~NonStd Res:~~]]~~</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene>~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=KEG:12-TUNGSTOPHOSPHATE'>KEG</scene></td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>SUB2, YDL084W ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~559292 Baker's yeast])</td~~><~~/tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5suq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5suq OCA], [https://pdbe.org/5suq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5suq RCSB], [https://www.ebi.ac.uk/pdbsum/5suq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5suq ProSAT]</span></td></tr>
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b></td><td class="sblockDat"><span class='plainlinks~~'>~~[http://en.wikipedia.org/wiki/RNA_helicase RNA helicase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.4.13 3.6.4.13]~~ </~~span~~></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5suq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5suq OCA], [~~http~~://pdbe.org/5suq PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5suq RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5suq PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5suq ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/SUB2_YEAST SUB2_YEAST]] ATP-binding RNA helicase component of the TREX complex involved in transcription elongation and required for the export of mRNA out of the nucleus. SUB2 plays also a role in pre-mRNA splicing and spliceosome assembly. May be involved in rDNA and telomeric silencing, and maintenance of genome integrity.<ref>PMID:10077188</ref> <ref>PMID:11156602</ref> <ref>PMID:11156603</ref> <ref>PMID:11156604</ref> <ref>PMID:11463828</ref> <ref>PMID:11675790</ref> <ref>PMID:11696331</ref> <ref>PMID:11979277</ref> <ref>PMID:12034490</ref> <ref>PMID:12093753</ref> <ref>PMID:12417727</ref> <ref>PMID:12871933</ref> <ref>PMID:15942929</ref>	+	[https://www.uniprot.org/uniprot/SUB2_YEAST SUB2_YEAST] ATP-binding RNA helicase component of the TREX complex involved in transcription elongation and required for the export of mRNA out of the nucleus. SUB2 plays also a role in pre-mRNA splicing and spliceosome assembly. May be involved in rDNA and telomeric silencing, and maintenance of genome integrity.<ref>PMID:10077188</ref> <ref>PMID:11156602</ref> <ref>PMID:11156603</ref> <ref>PMID:11156604</ref> <ref>PMID:11463828</ref> <ref>PMID:11675790</ref> <ref>PMID:11696331</ref> <ref>PMID:11979277</ref> <ref>PMID:12034490</ref> <ref>PMID:12093753</ref> <ref>PMID:12417727</ref> <ref>PMID:12871933</ref> <ref>PMID:15942929</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 28:		Line 26:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Atcc 18824]]
-	[[Category: Baker's yeast]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~RNA helicase~~]]	+	[[Category: Saccharomyces cerevisiae]]
-	[[Category: ~~Blobel, G~~]]	+	[[Category: Saccharomyces cerevisiae S288C]]
-	[[Category: ~~Ren, Y~~]]	+	[[Category: Blobel G]]
-	[[Category: ~~Hydrolase]]~~	+	[[Category: Ren Y]]
-	~~[[Category: Mrna export~~]]	+

OCA at 11:11, 16 September 2020

OCA — Wed, 16 Sep 2020 11:11:56 GMT

←Older revision		Revision as of 11:11, 16 September 2020
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	==Structure of mRNA export factors==		==Structure of mRNA export factors==
-	<StructureSection load='5suq' size='340' side='right' caption='[[5suq]], [[Resolution\|resolution]] 6.00Å' scene=''>	+	<StructureSection load='5suq' size='340' side='right'caption='[[5suq]], [[Resolution\|resolution]] 6.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5suq]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5SUQ OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5SUQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5suq]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824] and [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5SUQ OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5SUQ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=KEG:12-TUNGSTOPHOSPHATE'>KEG</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=KEG:12-TUNGSTOPHOSPHATE'>KEG</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SUB2, YDL084W ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/RNA_helicase RNA helicase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.4.13 3.6.4.13] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/RNA_helicase RNA helicase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.4.13 3.6.4.13] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5suq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5suq OCA], [http://pdbe.org/5suq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5suq RCSB], [http://www.ebi.ac.uk/pdbsum/5suq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5suq ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5suq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5suq OCA], [http://pdbe.org/5suq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5suq RCSB], [http://www.ebi.ac.uk/pdbsum/5suq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5suq ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 20:		Line 21:
	</div>		</div>
	<div class="pdbe-citations 5suq" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5suq" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Helicase 3D structures\|Helicase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Atcc 18824]]
		+	[[Category: Baker's yeast]]
		+	[[Category: Large Structures]]
	[[Category: RNA helicase]]		[[Category: RNA helicase]]
	[[Category: Blobel, G]]		[[Category: Blobel, G]]

OCA at 16:14, 18 January 2017

OCA — Wed, 18 Jan 2017 16:14:14 GMT

←Older revision		Revision as of 16:14, 18 January 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5suq is ~~ON HOLD~~ ~~until Paper~~ Publication	+	==Structure of mRNA export factors==
		+	<StructureSection load='5suq' size='340' side='right' caption='[[5suq]], [[Resolution\|resolution]] 6.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5suq]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5SUQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5SUQ FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=KEG:12-TUNGSTOPHOSPHATE'>KEG</scene></td></tr>
		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/RNA_helicase RNA helicase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.4.13 3.6.4.13] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5suq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5suq OCA], [http://pdbe.org/5suq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5suq RCSB], [http://www.ebi.ac.uk/pdbsum/5suq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5suq ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/SUB2_YEAST SUB2_YEAST]] ATP-binding RNA helicase component of the TREX complex involved in transcription elongation and required for the export of mRNA out of the nucleus. SUB2 plays also a role in pre-mRNA splicing and spliceosome assembly. May be involved in rDNA and telomeric silencing, and maintenance of genome integrity.<ref>PMID:10077188</ref> <ref>PMID:11156602</ref> <ref>PMID:11156603</ref> <ref>PMID:11156604</ref> <ref>PMID:11463828</ref> <ref>PMID:11675790</ref> <ref>PMID:11696331</ref> <ref>PMID:11979277</ref> <ref>PMID:12034490</ref> <ref>PMID:12093753</ref> <ref>PMID:12417727</ref> <ref>PMID:12871933</ref> <ref>PMID:15942929</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	mRNA is cotranscrptionally processed and packaged into messenger ribonucleoprotein particles (mRNPs) in the nucleus. Prior to export through the nuclear pore, mRNPs undergo several obligatory remodeling reactions. In yeast, one of these reactions involves loading of the mRNA-binding protein Yra1 by the DEAD-box ATPase Sub2 as assisted by the hetero-pentameric THO complex. To obtain molecular insights into reaction mechanisms, we determined crystal structures of two relevant complexes: a THO hetero-pentamer bound to Sub2 at 6.0 A resolution; and Sub2 associated with an ATP analogue, RNA, and a C-terminal fragment of Yra1 (Yra1-C) at 2.6 A resolution. We found that the 25 nm long THO clamps Sub2 in a half-open configuration; in contrast, when bound to the ATP analogue, RNA and Yra1-C, Sub2 assumes a closed conformation. Both THO and Yra1-C stimulated Sub2's intrinsic ATPase activity. We propose that THO surveys common landmarks in each nuclear mRNP to localize Sub2 for targeted loading of Yra1.

-	~~Authors~~:	+	Structural and biochemical analyses of the DEAD-box ATPase Sub2 in association with THO or Yra1.,Ren Y, Schmiege P, Blobel G Elife. 2017 Jan 6;6. pii: e20070. doi: 10.7554/eLife.20070. PMID:28059701<ref>PMID:28059701</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 5suq" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: RNA helicase]]
		+	[[Category: Blobel, G]]
		+	[[Category: Ren, Y]]
		+	[[Category: Hydrolase]]
		+	[[Category: Mrna export]]

OCA at 15:10, 19 October 2016

OCA — Wed, 19 Oct 2016 15:10:45 GMT

←Older revision		Revision as of 15:10, 19 October 2016
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5suq is ON HOLD	+	The entry 5suq is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "5suq" [edit=sysop:move=sysop]

OCA — Wed, 10 Aug 2016 14:42:58 GMT

Protected "5suq" [edit=sysop:move=sysop]

←Older revision

Revision as of 14:42, 10 August 2016

OCA: New page: '''Unreleased structure''' The entry 5suq is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 10 Aug 2016 14:42:57 GMT

New page: '''Unreleased structure''' The entry 5suq is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 5suq is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]