5swl - Revision history

OCA at 12:49, 4 October 2023

OCA — Wed, 04 Oct 2023 12:49:03 GMT

←Older revision		Revision as of 12:49, 4 October 2023
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	<StructureSection load='5swl' size='340' side='right'caption='[[5swl]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='5swl' size='340' side='right'caption='[[5swl]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5swl]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"shigella_paradysenteriae"_weldin_1927 "shigella paradysenteriae" weldin 1927~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5SWL OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5SWL FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5swl]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Shigella_flexneri Shigella flexneri]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5SWL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5SWL FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat"~~><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.7Å</td></tr>
-	~~<tr~~ id~~='related'><td class~~="~~sblockLbl~~">~~<b>~~[[~~Related_structure~~\|~~Related:]]</b></td><td class="sblockDat">[[5swj\|5swj~~]]</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>spaL, mxiB, spa47, CP0149 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~623~~ "~~Shigella paradysenteriae~~" ~~Weldin 1927])</td~~><~~/tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5swl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5swl OCA], [https://pdbe.org/5swl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5swl RCSB], [https://www.ebi.ac.uk/pdbsum/5swl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5swl ProSAT]</span></td></tr>
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b></td><td class="sblockDat"><span class='plainlinks~~'>~~[http://en.wikipedia.org/wiki/H(+)-transporting_two-sector_ATPase H(+)-transporting two-sector ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.3.14 3.6.3.14]~~ </~~span~~></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5swl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5swl OCA], [~~http~~://pdbe.org/5swl PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5swl RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5swl PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5swl ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/~~SPAL_SHIFL SPAL_SHIFL~~]~~] Required for surface presentation~~ of ~~invasion plasmid antigens~~. ~~Probable catalytic subunit of~~ a ~~protein translocase~~. Required for invasion and ~~for secretion~~ of the ~~three IPA proteins~~.	+	[https://www.uniprot.org/uniprot/SCTN_SHIFL SCTN_SHIFL] ATPase component of the type III secretion system (T3SS), also called injectisome, which is used to inject bacterial effector proteins into eukaryotic host cells (PubMed:26947936, PubMed:27770024, PubMed:29595954). Acts as a molecular motor to provide the energy that is required for the export of proteins (Probable). Required for type III secretion apparatus (T3SA) formation, proper protein secretion, host cell invasion and virulence (PubMed:26947936, PubMed:27770024, PubMed:31162724). May play a critical role in T3SS substrate recognition, disassembly of the effector/chaperone complex and unfolding of the effector in an ATP-dependent manner prior to secretion (By similarity).[UniProtKB:P0A1B9]<ref>PMID:26947936</ref> <ref>PMID:27770024</ref> <ref>PMID:29595954</ref> <ref>PMID:31162724</ref> <ref>PMID:26947936</ref> <ref>PMID:27770024</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Shigella paradysenteriae weldin 1927]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Burgess, J L~~]]	+	[[Category: Shigella flexneri]]
-	[[Category: Burgess~~, R A~~]]	+	[[Category: Burgess JL]]
-	[[Category: ~~Dickenson, N E~~]]	+	[[Category: Burgess RA]]
-	[[Category: ~~Johnson, S J~~]]	+	[[Category: Dickenson NE]]
-	[[Category: ~~Morales, Y~~]]	+	[[Category: Johnson SJ]]
-	[[Category: ~~Atpase]]~~	+	[[Category: Morales Y]]
-	~~[[Category: Hydrolase~~]]	+

OCA at 16:51, 11 December 2019

OCA — Wed, 11 Dec 2019 16:51:27 GMT

←Older revision		Revision as of 16:51, 11 December 2019
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	==Crystal Structure of ATPase delta1-79 Spa47 E188A==		==Crystal Structure of ATPase delta1-79 Spa47 E188A==
-	<StructureSection load='5swl' size='340' side='right' caption='[[5swl]], [[Resolution\|resolution]] 2.70Å' scene=''>	+	<StructureSection load='5swl' size='340' side='right'caption='[[5swl]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5swl]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"shigella_paradysenteriae"_weldin_1927 "shigella paradysenteriae" weldin 1927]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5SWL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5SWL FirstGlance]. <br>		<table><tr><td colspan='2'>[[5swl]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"shigella_paradysenteriae"_weldin_1927 "shigella paradysenteriae" weldin 1927]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5SWL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5SWL FirstGlance]. <br>
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	</StructureSection>		</StructureSection>
	[[Category: Shigella paradysenteriae weldin 1927]]		[[Category: Shigella paradysenteriae weldin 1927]]
		+	[[Category: Large Structures]]
	[[Category: Burgess, J L]]		[[Category: Burgess, J L]]
	[[Category: Burgess, R A]]		[[Category: Burgess, R A]]

OCA at 14:18, 6 November 2017

OCA — Mon, 06 Nov 2017 14:18:29 GMT

←Older revision		Revision as of 14:18, 6 November 2017
Line 3:		Line 3:
	<StructureSection load='5swl' size='340' side='right' caption='[[5swl]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='5swl' size='340' side='right' caption='[[5swl]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5swl]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5SWL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5SWL FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5swl]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"shigella_paradysenteriae"_weldin_1927 "shigella paradysenteriae" weldin 1927]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5SWL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5SWL FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5swj\|5swj]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5swj\|5swj]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">spaL, mxiB, spa47, CP0149 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=623 "Shigella paradysenteriae" Weldin 1927])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/H(+)-transporting_two-sector_ATPase H(+)-transporting two-sector ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.3.14 3.6.3.14] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/H(+)-transporting_two-sector_ATPase H(+)-transporting two-sector ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.3.14 3.6.3.14] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5swl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5swl OCA], [http://pdbe.org/5swl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5swl RCSB], [http://www.ebi.ac.uk/pdbsum/5swl PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5swl ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5swl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5swl OCA], [http://pdbe.org/5swl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5swl RCSB], [http://www.ebi.ac.uk/pdbsum/5swl PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5swl ProSAT]</span></td></tr>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Shigella paradysenteriae weldin 1927]]
	[[Category: Burgess, J L]]		[[Category: Burgess, J L]]
	[[Category: Burgess, R A]]		[[Category: Burgess, R A]]

OCA at 18:16, 2 November 2016

OCA — Wed, 02 Nov 2016 18:16:27 GMT

←Older revision		Revision as of 18:16, 2 November 2016
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5swl is ~~ON HOLD until Paper~~ Publication	+	==Crystal Structure of ATPase delta1-79 Spa47 E188A==
		+	<StructureSection load='5swl' size='340' side='right' caption='[[5swl]], [[Resolution\|resolution]] 2.70Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5swl]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5SWL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5SWL FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5swj\|5swj]]</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/H(+)-transporting_two-sector_ATPase H(+)-transporting two-sector ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.3.14 3.6.3.14] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5swl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5swl OCA], [http://pdbe.org/5swl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5swl RCSB], [http://www.ebi.ac.uk/pdbsum/5swl PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5swl ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/SPAL_SHIFL SPAL_SHIFL]] Required for surface presentation of invasion plasmid antigens. Probable catalytic subunit of a protein translocase. Required for invasion and for secretion of the three IPA proteins.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Like many Gram-negative pathogens, Shigella rely on a complex type three secretion system (T3SS) to inject effector proteins into host cells, take over host functions, and ultimately establish infection. Despite these critical roles, the energetics and regulatory mechanisms controlling the T3SS and pathogen virulence remain largely unclear. In this study, we present a series of high-resolution crystal structures of Spa47 and use the structures to model an activated Spa47 oligomer, finding that ATP hydrolysis may be supported by specific sidechain contributions from adjacent protomers within the complex. Follow-up mutagenesis experiments targeting the predicted active site residues validate the oligomeric model and determined that each of the tested residues are essential for Spa47 ATPase activity, though they are not directly responsible for stable oligomer formation. While an N-terminal domain truncation was necessary for crystal formation, it resulted in strictly monomeric Spa47 that is unable to hydrolyze ATP, despite maintaining the canonical ATPase core structure and active site residues. Coupled with studies of ATPase inactive full-length Spa47 point mutants, we find that Spa47 oligomerization and ATP hydrolysis are needed for complete T3SS apparatus formation, a proper translocator secretion profile, and Shigella virulence. This work represents the first structure-function characterization of Spa47, uniquely complementing the multitude of included Shigella T3SS phenotype assays and providing a more complete understanding of T3SS ATPase-mediated pathogen virulence. Additionally, these findings provide a strong platform for follow-up studies evaluating regulation of Spa47 oligomerization in vivo as a much needed means of treating and perhaps preventing shigellosis.

-	~~Authors:~~ Morales, Y., Johnson, S.J.~~, Burgess, J~~.L.~~, Burgess, R~~.~~A., Dickenson, N.E~~.	+	Structural and Biochemical Characterization of Spa47 Provides Mechanistic Insight into Type III Secretion System ATPase Activation and Shigella Virulence Regulation.,Burgess JL, Burgess RA, Morales Y, Bouvang JM, Johnson SJ, Dickenson NE J Biol Chem. 2016 Oct 21. pii: jbc.M116.755256. PMID:27770024<ref>PMID:27770024</ref>

-	~~Description: Crystal Structure~~ of ~~ATPase delta1~~-~~79 Spa47 E188A~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
-	[[Category: ~~Dickenson~~, ~~N.E~~]]	+	<div class="pdbe-citations 5swl" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Burgess~~, ~~R.A~~]]	+	== References ==
-	[[Category: Johnson, S.J]]	+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Burgess, J L]]
		+	[[Category: Burgess, R A]]
		+	[[Category: Dickenson, N E]]
		+	[[Category: Johnson, S J]]
	[[Category: Morales, Y]]		[[Category: Morales, Y]]
-	[[Category: ~~Burgess, J.L~~]]	+	[[Category: Atpase]]
		+	[[Category: Hydrolase]]

OCA at 13:47, 14 September 2016

OCA — Wed, 14 Sep 2016 13:47:34 GMT

←Older revision		Revision as of 13:47, 14 September 2016
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5swl is ON HOLD	+	The entry 5swl is ON HOLD until Paper Publication

	Authors: Morales, Y., Johnson, S.J., Burgess, J.L., Burgess, R.A., Dickenson, N.E.		Authors: Morales, Y., Johnson, S.J., Burgess, J.L., Burgess, R.A., Dickenson, N.E.

OCA: Protected "5swl" [edit=sysop:move=sysop]

OCA — Fri, 09 Sep 2016 15:21:48 GMT

Protected "5swl" [edit=sysop:move=sysop]

←Older revision

Revision as of 15:21, 9 September 2016

OCA: New page: '''Unreleased structure''' The entry 5swl is ON HOLD Authors: Morales, Y., Johnson, S.J., Burgess, J.L., Burgess, R.A., Dickenson, N.E. Description: Crystal Structure of ATPase delta1-...

OCA — Fri, 09 Sep 2016 15:21:46 GMT

New page: '''Unreleased structure''' The entry 5swl is ON HOLD Authors: Morales, Y., Johnson, S.J., Burgess, J.L., Burgess, R.A., Dickenson, N.E. Description: Crystal Structure of ATPase delta1-...

New page

'''Unreleased structure'''

The entry 5swl is ON HOLD

Authors: Morales, Y., Johnson, S.J., Burgess, J.L., Burgess, R.A., Dickenson, N.E.

Description: Crystal Structure of ATPase delta1-79 Spa47 E188A
[[Category: Unreleased Structures]]
[[Category: Dickenson, N.E]]
[[Category: Burgess, R.A]]
[[Category: Johnson, S.J]]
[[Category: Morales, Y]]
[[Category: Burgess, J.L]]