5tfy - Revision history

OCA at 15:40, 6 March 2024

OCA — Wed, 06 Mar 2024 15:40:48 GMT

←Older revision		Revision as of 15:40, 6 March 2024
Line 3:		Line 3:
	<SX load='5tfy' size='340' side='right' viewer='molstar' caption='[[5tfy]], [[Resolution\|resolution]] 3.40Å' scene=''>		<SX load='5tfy' size='340' side='right' viewer='molstar' caption='[[5tfy]], [[Resolution\|resolution]] 3.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5tfy]] is a 26 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Methj Methj~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5TFY OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5TFY FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5tfy]] is a 26 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanospirillum_hungatei_JF-1 Methanospirillum hungatei JF-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5TFY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5TFY FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5tfy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5tfy OCA], [~~http~~://pdbe.org/5tfy PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5tfy RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5tfy PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5tfy ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 3.4Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5tfy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5tfy OCA], [https://pdbe.org/5tfy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5tfy RCSB], [https://www.ebi.ac.uk/pdbsum/5tfy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5tfy ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/Q2FUM4_METHJ Q2FUM4_METHJ]] Flagellin is the subunit protein which polymerizes to form the filaments of archaeal flagella.[RuleBase:RU361282]	+	[https://www.uniprot.org/uniprot/Q2FUM4_METHJ Q2FUM4_METHJ] Flagellin is the subunit protein which polymerizes to form the filaments of archaeal flagella.[RuleBase:RU361282]
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	Archaea use flagella known as archaella-distinct both in protein composition and structure from bacterial flagella-to drive cell motility, but the structural basis of this function is unknown. Here, we report an atomic model of the archaella, based on the cryo electron microscopy (cryoEM) structure of the Methanospirillum hungatei archaellum at 3.4 A resolution. Each archaellum contains approximately 61,500 archaellin subunits organized into a curved helix with a diameter of 10 nm and average length of 10,000 nm. The tadpole-shaped archaellin monomer has two domains, a beta-barrel domain and a long, mildly kinked alpha-helix tail. Our structure reveals multiple post-translational modifications to the archaella, including six O-linked glycans and an unusual N-linked modification. The extensive interactions among neighbouring archaellins explain how the long but thin archaellum maintains the structural integrity required for motility-driving rotation. These extensive inter-subunit interactions and the absence of a central pore in the archaellum distinguish it from both the bacterial flagellum and type IV pili.	+
-		+
-	CryoEM structure of the Methanospirillum hungatei archaellum reveals structural features distinct from the bacterial flagellum and type IV pili.,Poweleit N, Ge P, Nguyen HH, Loo RR, Gunsalus RP, Zhou ZH Nat Microbiol. 2016 Dec 5;2:16222. doi: 10.1038/nmicrobiol.2016.222. PMID:27922015<ref>PMID:27922015</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 5tfy" style="background-color:#fffaf0;"></div>~~	+

	==See Also==		==See Also==
	*[[Flagellin 3D structures\|Flagellin 3D structures]]		*[[Flagellin 3D structures\|Flagellin 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</SX>		</SX>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Methj~~]]	+	[[Category: Methanospirillum hungatei JF-1]]
-	[[Category: Gunsalus~~, R P~~]]	+	[[Category: Gunsalus RP]]
-	[[Category: Peng, G]]	+	[[Category: Peng G]]
-	[[Category: Poweleit, N]]	+	[[Category: Poweleit N]]
-	[[Category: Zhou~~, Z H]]~~	+	[[Category: Zhou ZH]]
-	~~[[Category: Cell adhesion]]~~	+
-	~~[[Category: Cell motility and adhesion~~]]	+

OCA at 21:44, 10 April 2020

OCA — Fri, 10 Apr 2020 21:44:48 GMT

←Older revision		Revision as of 21:44, 10 April 2020
Line 3:		Line 3:
	<SX load='5tfy' size='340' side='right' viewer='molstar' caption='[[5tfy]], [[Resolution\|resolution]] 3.40Å' scene=''>		<SX load='5tfy' size='340' side='right' viewer='molstar' caption='[[5tfy]], [[Resolution\|resolution]] 3.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5tfy]] is a 26 chain structure with sequence from [http://en.wikipedia.org/wiki/Methj Methj]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5TFY OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5TFY FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5tfy]] is a 26 chain structure with sequence from [http://en.wikipedia.org/wiki/Methj Methj]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5TFY OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5TFY FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5tfy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5tfy OCA], [http://pdbe.org/5tfy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5tfy RCSB], [http://www.ebi.ac.uk/pdbsum/5tfy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5tfy ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5tfy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5tfy OCA], [http://pdbe.org/5tfy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5tfy RCSB], [http://www.ebi.ac.uk/pdbsum/5tfy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5tfy ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 19:36, 6 March 2020

OCA — Fri, 06 Mar 2020 19:36:52 GMT

←Older revision		Revision as of 19:36, 6 March 2020
Line 1:		Line 1:

	==The archaeal flagellum of Methanospirillum hungatei strain JF1.==		==The archaeal flagellum of Methanospirillum hungatei strain JF1.==
-	<~~StructureSection~~ load='5tfy' size='340' side='right'caption='[[5tfy]], [[Resolution\|resolution]] 3.40Å' scene=''>	+	<SX load='5tfy' size='340' side='right' viewer='molstar' caption='[[5tfy]], [[Resolution\|resolution]] 3.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5tfy]] is a 26 chain structure with sequence from [http://en.wikipedia.org/wiki/Methj Methj]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5TFY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5TFY FirstGlance]. <br>		<table><tr><td colspan='2'>[[5tfy]] is a 26 chain structure with sequence from [http://en.wikipedia.org/wiki/Methj Methj]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5TFY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5TFY FirstGlance]. <br>
Line 23:		Line 23:
	<references/>		<references/>
	__TOC__		__TOC__
-	</~~StructureSection~~>	+	</SX>
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Methj]]		[[Category: Methj]]

OCA at 07:52, 27 November 2019

OCA — Wed, 27 Nov 2019 07:52:50 GMT

←Older revision		Revision as of 07:52, 27 November 2019
Line 1:		Line 1:

	==The archaeal flagellum of Methanospirillum hungatei strain JF1.==		==The archaeal flagellum of Methanospirillum hungatei strain JF1.==
-	<StructureSection load='5tfy' size='340' side='right' caption='[[5tfy]], [[Resolution\|resolution]] 3.40Å' scene=''>	+	<StructureSection load='5tfy' size='340' side='right'caption='[[5tfy]], [[Resolution\|resolution]] 3.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5tfy]] is a 26 chain structure with sequence from [http://en.wikipedia.org/wiki/Methj Methj]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5TFY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5TFY FirstGlance]. <br>		<table><tr><td colspan='2'>[[5tfy]] is a 26 chain structure with sequence from [http://en.wikipedia.org/wiki/Methj Methj]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5TFY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5TFY FirstGlance]. <br>
Line 17:		Line 17:
	</div>		</div>
	<div class="pdbe-citations 5tfy" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5tfy" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Flagellin 3D structures\|Flagellin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Methj]]		[[Category: Methj]]
	[[Category: Gunsalus, R P]]		[[Category: Gunsalus, R P]]

OCA at 10:21, 8 November 2017

OCA — Wed, 08 Nov 2017 10:21:12 GMT

←Older revision		Revision as of 10:21, 8 November 2017
Line 3:		Line 3:
	<StructureSection load='5tfy' size='340' side='right' caption='[[5tfy]], [[Resolution\|resolution]] 3.40Å' scene=''>		<StructureSection load='5tfy' size='340' side='right' caption='[[5tfy]], [[Resolution\|resolution]] 3.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5tfy]] is a 26 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Methanospirillum_hungatei_jf-1_(strain_atcc_27890_/_dsm_864_/_nbrc_100397_/_jf-1) Methanospirillum hungatei jf-1 (strain atcc 27890 / dsm 864 / nbrc 100397 / jf-1)~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5TFY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5TFY FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5tfy]] is a 26 chain structure with sequence from [http://en.wikipedia.org/wiki/Methj Methj]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5TFY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5TFY FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5tfy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5tfy OCA], [http://pdbe.org/5tfy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5tfy RCSB], [http://www.ebi.ac.uk/pdbsum/5tfy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5tfy ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5tfy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5tfy OCA], [http://pdbe.org/5tfy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5tfy RCSB], [http://www.ebi.ac.uk/pdbsum/5tfy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5tfy ProSAT]</span></td></tr>
	</table>		</table>
Line 21:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Methj]]
	[[Category: Gunsalus, R P]]		[[Category: Gunsalus, R P]]
	[[Category: Peng, G]]		[[Category: Peng, G]]

OCA at 07:43, 18 January 2017

OCA — Wed, 18 Jan 2017 07:43:37 GMT

←Older revision		Revision as of 07:43, 18 January 2017
Line 8:		Line 8:
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/Q2FUM4_METHJ Q2FUM4_METHJ]] Flagellin is the subunit protein which polymerizes to form the filaments of archaeal flagella.[RuleBase:RU361282]		[[http://www.uniprot.org/uniprot/Q2FUM4_METHJ Q2FUM4_METHJ]] Flagellin is the subunit protein which polymerizes to form the filaments of archaeal flagella.[RuleBase:RU361282]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Archaea use flagella known as archaella-distinct both in protein composition and structure from bacterial flagella-to drive cell motility, but the structural basis of this function is unknown. Here, we report an atomic model of the archaella, based on the cryo electron microscopy (cryoEM) structure of the Methanospirillum hungatei archaellum at 3.4 A resolution. Each archaellum contains approximately 61,500 archaellin subunits organized into a curved helix with a diameter of 10 nm and average length of 10,000 nm. The tadpole-shaped archaellin monomer has two domains, a beta-barrel domain and a long, mildly kinked alpha-helix tail. Our structure reveals multiple post-translational modifications to the archaella, including six O-linked glycans and an unusual N-linked modification. The extensive interactions among neighbouring archaellins explain how the long but thin archaellum maintains the structural integrity required for motility-driving rotation. These extensive inter-subunit interactions and the absence of a central pore in the archaellum distinguish it from both the bacterial flagellum and type IV pili.
		+
		+	CryoEM structure of the Methanospirillum hungatei archaellum reveals structural features distinct from the bacterial flagellum and type IV pili.,Poweleit N, Ge P, Nguyen HH, Loo RR, Gunsalus RP, Zhou ZH Nat Microbiol. 2016 Dec 5;2:16222. doi: 10.1038/nmicrobiol.2016.222. PMID:27922015<ref>PMID:27922015</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5tfy" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 02:19, 11 December 2016

OCA — Sun, 11 Dec 2016 02:19:42 GMT

←Older revision		Revision as of 02:19, 11 December 2016
Line 1:		Line 1:
-	'''Unreleased structure'''

-	The ~~entry~~ 5tfy ~~is ON HOLD until Paper Publication~~	+	==The archaeal flagellum of Methanospirillum hungatei strain JF1.==
-		+	<StructureSection load='5tfy' size='340' side='right' caption='[[5tfy]], [[Resolution\|resolution]] 3.40Å' scene=''>
-	~~Authors~~: ~~Poweleit~~, N., ~~Peng~~, G., ~~Gunsalus, R~~.P., ~~Zhou, Z~~.H.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5tfy]] is a 26 chain structure with sequence from [http://en.wikipedia.org/wiki/Methanospirillum_hungatei_jf-1_(strain_atcc_27890_/_dsm_864_/_nbrc_100397_/_jf-1) Methanospirillum hungatei jf-1 (strain atcc 27890 / dsm 864 / nbrc 100397 / jf-1)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5TFY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5TFY FirstGlance]. <br>
-	~~Description~~: ~~The archaeal flagellum~~ of ~~Methanospirillum hungatei strain JF1~~.	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5tfy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5tfy OCA], [http://pdbe.org/5tfy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5tfy RCSB], [http://www.ebi.ac.uk/pdbsum/5tfy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5tfy ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/Q2FUM4_METHJ Q2FUM4_METHJ]] Flagellin is the subunit protein which polymerizes to form the filaments of archaeal flagella.[RuleBase:RU361282]
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Gunsalus, R P]]
		+	[[Category: Peng, G]]
	[[Category: Poweleit, N]]		[[Category: Poweleit, N]]
-	[[Category: ~~Peng~~, G]]	+	[[Category: Zhou, Z H]]
-	[[Category: ~~Zhou, Z.H~~]]	+	[[Category: Cell adhesion]]
-	[[Category: ~~Gunsalus, R.P~~]]	+	[[Category: Cell motility and adhesion]]

OCA at 15:45, 19 October 2016

OCA — Wed, 19 Oct 2016 15:45:43 GMT

←Older revision		Revision as of 15:45, 19 October 2016
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5tfy is ON HOLD	+	The entry 5tfy is ON HOLD until Paper Publication

	Authors: Poweleit, N., Peng, G., Gunsalus, R.P., Zhou, Z.H.		Authors: Poweleit, N., Peng, G., Gunsalus, R.P., Zhou, Z.H.

OCA: Protected "5tfy" [edit=sysop:move=sysop]

OCA — Wed, 05 Oct 2016 20:01:50 GMT

Protected "5tfy" [edit=sysop:move=sysop]

←Older revision

Revision as of 20:01, 5 October 2016

OCA: New page: '''Unreleased structure''' The entry 5tfy is ON HOLD Authors: Poweleit, N., Peng, G., Gunsalus, R.P., Zhou, Z.H. Description: The archaeal flagellum of Methanospirillum hungatei strain...

OCA — Wed, 05 Oct 2016 20:01:49 GMT

New page: '''Unreleased structure''' The entry 5tfy is ON HOLD Authors: Poweleit, N., Peng, G., Gunsalus, R.P., Zhou, Z.H. Description: The archaeal flagellum of Methanospirillum hungatei strain...

New page

'''Unreleased structure'''

The entry 5tfy is ON HOLD

Authors: Poweleit, N., Peng, G., Gunsalus, R.P., Zhou, Z.H.

Description: The archaeal flagellum of Methanospirillum hungatei strain JF1.
[[Category: Unreleased Structures]]
[[Category: Poweleit, N]]
[[Category: Peng, G]]
[[Category: Zhou, Z.H]]
[[Category: Gunsalus, R.P]]