5tha - Revision history

OCA at 10:02, 21 December 2022

OCA — Wed, 21 Dec 2022 10:02:24 GMT

←Older revision		Revision as of 10:02, 21 December 2022
Line 3:		Line 3:
	<StructureSection load='5tha' size='340' side='right'caption='[[5tha]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='5tha' size='340' side='right'caption='[[5tha]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5tha]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5THA OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5THA FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5tha]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5THA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5THA FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GP3:DIGUANOSINE-5-TRIPHOSPHATE'>GP3</scene>, <scene name='pdbligand=UNX:UNKNOWN+ATOM+OR+ION'>UNX</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GP3:DIGUANOSINE-5-TRIPHOSPHATE'>GP3</scene>, <scene name='pdbligand=UNX:UNKNOWN+ATOM+OR+ION'>UNX</scene></td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GEMIN5 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5tha FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5tha OCA], [https://pdbe.org/5tha PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5tha RCSB], [https://www.ebi.ac.uk/pdbsum/5tha PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5tha ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5tha FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5tha OCA], [~~http~~://pdbe.org/5tha PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5tha RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5tha PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5tha ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/GEMI5_HUMAN GEMI5_HUMAN]] The SMN complex plays a catalyst role in the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome. Thereby, plays an important role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP. In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP. Dissociation by the SMN complex of CLNS1A from the trapped Sm proteins and their transfer to an SMN-Sm complex triggers the assembly of core snRNPs and their transport to the nucleus. GEMIN5 acts as the snRNA-binding protein of the SMN complex.<ref>PMID:11714716</ref> <ref>PMID:16857593</ref> <ref>PMID:18984161</ref>	+	[https://www.uniprot.org/uniprot/GEMI5_HUMAN GEMI5_HUMAN] The SMN complex plays a catalyst role in the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome. Thereby, plays an important role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP. In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP. Dissociation by the SMN complex of CLNS1A from the trapped Sm proteins and their transfer to an SMN-Sm complex triggers the assembly of core snRNPs and their transport to the nucleus. GEMIN5 acts as the snRNA-binding protein of the SMN complex.<ref>PMID:11714716</ref> <ref>PMID:16857593</ref> <ref>PMID:18984161</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Arrowsmith~~, C H~~]]	+	[[Category: Arrowsmith CH]]
-	[[Category: Bian, C]]	+	[[Category: Bian C]]
-	[[Category: Bountra, C]]	+	[[Category: Bountra C]]
-	[[Category: Cerovina, T]]	+	[[Category: Cerovina T]]
-	[[Category: Chao, X]]	+	[[Category: Chao X]]
-	[[Category: Edwards~~, A M~~]]	+	[[Category: Edwards AM]]
-	[[Category: He, H]]	+	[[Category: He H]]
-	[[Category: Min, J]]	+	[[Category: Min J]]
-	~~[[Category: Structural genomic~~]]	+	[[Category: Seitova A]]
-	[[Category: Seitova, A]]	+	[[Category: Tempel W]]
-	[[Category: Tempel, W]]	+
-	~~[[Category: Rna binding protein]]~~	+
-	~~[[Category: Sgc]]~~	+
-	~~[[Category: Wd40 repeat~~]]	+

OCA at 12:10, 23 September 2020

OCA — Wed, 23 Sep 2020 12:10:59 GMT

←Older revision		Revision as of 12:10, 23 September 2020
Line 1:		Line 1:

	==Gemin5 WD40 repeats in complex with a guanosyl moiety==		==Gemin5 WD40 repeats in complex with a guanosyl moiety==
-	<StructureSection load='5tha' size='340' side='right' caption='[[5tha]], [[Resolution\|resolution]] 1.80Å' scene=''>	+	<StructureSection load='5tha' size='340' side='right'caption='[[5tha]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5tha]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5THA OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5THA FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5tha]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5THA OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5THA FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GP3:DIGUANOSINE-5-TRIPHOSPHATE'>GP3</scene>, <scene name='pdbligand=UNX:UNKNOWN+ATOM+OR+ION'>UNX</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GP3:DIGUANOSINE-5-TRIPHOSPHATE'>GP3</scene>, <scene name='pdbligand=UNX:UNKNOWN+ATOM+OR+ION'>UNX</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5tha FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5tha OCA], [http://pdbe.org/5tha PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5tha RCSB], [http://www.ebi.ac.uk/pdbsum/5tha PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5tha ProSAT]</span></td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GEMIN5 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5tha FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5tha OCA], [http://pdbe.org/5tha PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5tha RCSB], [http://www.ebi.ac.uk/pdbsum/5tha PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5tha ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 22:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Arrowsmith, C H]]		[[Category: Arrowsmith, C H]]
	[[Category: Bian, C]]		[[Category: Bian, C]]

OCA at 06:16, 10 May 2017

OCA — Wed, 10 May 2017 06:16:19 GMT

←Older revision		Revision as of 06:16, 10 May 2017
Line 31:		Line 31:
	[[Category: Min, J]]		[[Category: Min, J]]
	[[Category: Structural genomic]]		[[Category: Structural genomic]]
		+	[[Category: Seitova, A]]
	[[Category: Tempel, W]]		[[Category: Tempel, W]]
	[[Category: Rna binding protein]]		[[Category: Rna binding protein]]
	[[Category: Sgc]]		[[Category: Sgc]]
	[[Category: Wd40 repeat]]		[[Category: Wd40 repeat]]

OCA at 07:23, 18 January 2017

OCA — Wed, 18 Jan 2017 07:23:02 GMT

←Older revision		Revision as of 07:23, 18 January 2017
Line 9:		Line 9:
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/GEMI5_HUMAN GEMI5_HUMAN]] The SMN complex plays a catalyst role in the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome. Thereby, plays an important role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP. In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP. Dissociation by the SMN complex of CLNS1A from the trapped Sm proteins and their transfer to an SMN-Sm complex triggers the assembly of core snRNPs and their transport to the nucleus. GEMIN5 acts as the snRNA-binding protein of the SMN complex.<ref>PMID:11714716</ref> <ref>PMID:16857593</ref> <ref>PMID:18984161</ref>		[[http://www.uniprot.org/uniprot/GEMI5_HUMAN GEMI5_HUMAN]] The SMN complex plays a catalyst role in the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome. Thereby, plays an important role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP. In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP. Dissociation by the SMN complex of CLNS1A from the trapped Sm proteins and their transfer to an SMN-Sm complex triggers the assembly of core snRNPs and their transport to the nucleus. GEMIN5 acts as the snRNA-binding protein of the SMN complex.<ref>PMID:11714716</ref> <ref>PMID:16857593</ref> <ref>PMID:18984161</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	In cytoplasm, the survival of motor neuron (SMN) complex delivers pre-small nuclear RNAs (pre-snRNAs) to the heptameric Sm ring for the assembly of the ring complex on pre-snRNAs at the conserved Sm site [A(U)4-6G]. Gemin5, a WD40 protein component of the SMN complex, is responsible for recognizing pre-snRNAs. In addition, Gemin5 has been reported to specifically bind to the m7G cap. In this study, we show that the WD40 domain of Gemin5 is both necessary and sufficient for binding the Sm site of pre-snRNAs by isothermal titration calorimetry (ITC) and mutagenesis assays. We further determined the crystal structures of the WD40 domain of Gemin5 in complex with the Sm site or m7G cap of pre-snRNA, which reveal that the WD40 domain of Gemin5 recognizes the Sm site and m7G cap of pre-snRNAs via two distinct binding sites by respective base-specific interactions. In addition, we also uncovered a novel role of Gemin5 in escorting the truncated forms of U1 pre-snRNAs for proper disposal. Overall, the elucidated Gemin5 structures will contribute to a better understanding of Gemin5 in small nuclear ribonucleic protein (snRNP) biogenesis as well as, potentially, other cellular activities.
		+
		+	Structural insights into Gemin5-guided selection of pre-snRNAs for snRNP assembly.,Xu C, Ishikawa H, Izumikawa K, Li L, He H, Nobe Y, Yamauchi Y, Shahjee HM, Wu XH, Yu YT, Isobe T, Takahashi N, Min J Genes Dev. 2016 Nov 1;30(21):2376-2390. Epub 2016 Nov 10. PMID:27881600<ref>PMID:27881600</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5tha" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 01:04, 20 October 2016

OCA — Thu, 20 Oct 2016 01:04:14 GMT

←Older revision		Revision as of 01:04, 20 October 2016
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5tha is ~~ON HOLD~~	+	==Gemin5 WD40 repeats in complex with a guanosyl moiety==
-		+	<StructureSection load='5tha' size='340' side='right' caption='[[5tha]], [[Resolution\|resolution]] 1.80Å' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5tha]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5THA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5THA FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GP3:DIGUANOSINE-5-TRIPHOSPHATE'>GP3</scene>, <scene name='pdbligand=UNX:UNKNOWN+ATOM+OR+ION'>UNX</scene></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5tha FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5tha OCA], [http://pdbe.org/5tha PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5tha RCSB], [http://www.ebi.ac.uk/pdbsum/5tha PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5tha ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/GEMI5_HUMAN GEMI5_HUMAN]] The SMN complex plays a catalyst role in the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome. Thereby, plays an important role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP. In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP. Dissociation by the SMN complex of CLNS1A from the trapped Sm proteins and their transfer to an SMN-Sm complex triggers the assembly of core snRNPs and their transport to the nucleus. GEMIN5 acts as the snRNA-binding protein of the SMN complex.<ref>PMID:11714716</ref> <ref>PMID:16857593</ref> <ref>PMID:18984161</ref>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Arrowsmith, C H]]
		+	[[Category: Bian, C]]
		+	[[Category: Bountra, C]]
		+	[[Category: Cerovina, T]]
		+	[[Category: Chao, X]]
		+	[[Category: Edwards, A M]]
		+	[[Category: He, H]]
		+	[[Category: Min, J]]
		+	[[Category: Structural genomic]]
		+	[[Category: Tempel, W]]
		+	[[Category: Rna binding protein]]
		+	[[Category: Sgc]]
		+	[[Category: Wd40 repeat]]

OCA: Protected "5tha" [edit=sysop:move=sysop]

OCA — Wed, 05 Oct 2016 20:05:44 GMT

Protected "5tha" [edit=sysop:move=sysop]

←Older revision

Revision as of 20:05, 5 October 2016

OCA: New page: '''Unreleased structure''' The entry 5tha is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 05 Oct 2016 20:05:43 GMT

New page: '''Unreleased structure''' The entry 5tha is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 5tha is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]