5tsy - Revision history

OCA at 13:10, 4 October 2023

OCA — Wed, 04 Oct 2023 13:10:57 GMT

←Older revision		Revision as of 13:10, 4 October 2023
Line 3:		Line 3:
	<StructureSection load='5tsy' size='340' side='right'caption='[[5tsy]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='5tsy' size='340' side='right'caption='[[5tsy]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5tsy]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Pseae Pseae~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5TSY OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5TSY FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5tsy]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5TSY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5TSY FirstGlance]. <br>
-	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~pelA~~, ~~PA3064 (~~[~~http://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=208964 PSEAE])~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.9Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5tsy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5tsy OCA], [~~http~~://pdbe.org/5tsy PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5tsy RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5tsy PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5tsy ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5tsy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5tsy OCA], [https://pdbe.org/5tsy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5tsy RCSB], [https://www.ebi.ac.uk/pdbsum/5tsy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5tsy ProSAT]</span></td></tr>
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/Q9HZE4_PSEAE Q9HZE4_PSEAE]
-	Bacterial biofilms present a significant medical challenge because they are recalcitrant to current therapeutic regimes. A key component of biofilm formation in the opportunistic human pathogen Pseudomonas aeruginosa is the biosynthesis of the exopolysaccharides Pel and Psl, which are involved in the formation and maintenance of the structural biofilm scaffold and protection against antimicrobials and host defenses. Given that the glycoside hydrolases PelAh and PslGh encoded in the pel and psl biosynthetic operons, respectively, are utilized for in vivo exopolysaccharide processing, we reasoned that these would provide specificity to target P. aeruginosa biofilms. Evaluating these enzymes as potential therapeutics, we demonstrate that these glycoside hydrolases selectively target and degrade the exopolysaccharide component of the biofilm matrix. PelAh and PslGh inhibit biofilm formation over a 24-hour period with a half maximal effective concentration (EC50) of 69.3 +/- 1.2 and 4.1 +/- 1.1 nM, respectively, and are capable of disrupting preexisting biofilms in 1 hour with EC50 of 35.7 +/- 1.1 and 12.9 +/- 1.1 nM, respectively. This treatment was effective against clinical and environmental P. aeruginosa isolates and reduced biofilm biomass by 58 to 94%. These noncytotoxic enzymes potentiated antibiotics because the addition of either enzyme to a sublethal concentration of colistin reduced viable bacterial counts by 2.5 orders of magnitude when used either prophylactically or on established 24-hour biofilms. In addition, PelAh was able to increase neutrophil killing by ~50%. This work illustrates the feasibility and benefits of using bacterial exopolysaccharide biosynthetic glycoside hydrolases to develop novel antibiofilm therapeutics.	+
-		+
-	Exopolysaccharide biosynthetic glycoside hydrolases can be utilized to disrupt and prevent Pseudomonas aeruginosa biofilms.,Baker P, Hill PJ, Snarr BD, Alnabelseya N, Pestrak MJ, Lee MJ, Jennings LK, Tam J, Melnyk RA, Parsek MR, Sheppard DC, Wozniak DJ, Howell PL Sci Adv. 2016 May 20;2(5):~~e1501632. doi: 10.1126~~/~~sciadv.1501632. eCollection 2016, May. PMID:27386527<ref>PMID:27386527<~~/~~ref>~~	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U~~.S. ~~National Library of Medicine.<br>~~	+
-	</~~div>~~	+
-	~~<div class="pdbe-citations 5tsy" style="background-color:#fffaf0;"></div>~~	+
-	~~== References ==~~	+
-	~~<references~~/>	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Pseae~~]]	+	[[Category: Pseudomonas aeruginosa PAO1]]
-	[[Category: Baker, P]]	+	[[Category: Baker P]]
-	[[Category: Howell~~, P L~~]]	+	[[Category: Howell PL]]
-	[[Category: Pfoh, R]]	+	[[Category: Pfoh R]]
-	~~[[Category: Glycoside hydrolase]]~~	+
-	~~[[Category: Hydrolase~~]]	+

OCA at 08:19, 8 January 2020

OCA — Wed, 08 Jan 2020 08:19:34 GMT

←Older revision		Revision as of 08:19, 8 January 2020
Line 1:		Line 1:

	==Structure of the glycoside hydrolase domain of PelA variant E218A from Pseudomonas aeruginosa==		==Structure of the glycoside hydrolase domain of PelA variant E218A from Pseudomonas aeruginosa==
-	<StructureSection load='5tsy' size='340' side='right' caption='[[5tsy]], [[Resolution\|resolution]] 1.90Å' scene=''>	+	<StructureSection load='5tsy' size='340' side='right'caption='[[5tsy]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5tsy]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseae Pseae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5TSY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5TSY FirstGlance]. <br>		<table><tr><td colspan='2'>[[5tsy]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseae Pseae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5TSY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5TSY FirstGlance]. <br>
Line 20:		Line 20:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Pseae]]		[[Category: Pseae]]
	[[Category: Baker, P]]		[[Category: Baker, P]]

OCA at 06:12, 8 March 2018

OCA — Thu, 08 Mar 2018 06:12:07 GMT

←Older revision		Revision as of 06:12, 8 March 2018
Line 3:		Line 3:
	<StructureSection load='5tsy' size='340' side='right' caption='[[5tsy]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='5tsy' size='340' side='right' caption='[[5tsy]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5tsy]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5TSY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5TSY FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5tsy]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseae Pseae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5TSY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5TSY FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5tsy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5tsy OCA], [http://pdbe.org/5tsy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5tsy RCSB], [http://www.ebi.ac.uk/pdbsum/5tsy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5tsy ProSAT]</span></td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">pelA, PA3064 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208964 PSEAE])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5tsy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5tsy OCA], [http://pdbe.org/5tsy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5tsy RCSB], [http://www.ebi.ac.uk/pdbsum/5tsy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5tsy ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 19:		Line 20:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Pseae]]
	[[Category: Baker, P]]		[[Category: Baker, P]]
	[[Category: Howell, P L]]		[[Category: Howell, P L]]

OCA at 07:49, 8 November 2017

OCA — Wed, 08 Nov 2017 07:49:38 GMT

←Older revision		Revision as of 07:49, 8 November 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5tsy is ~~ON HOLD until Paper~~ Publication	+	==Structure of the glycoside hydrolase domain of PelA variant E218A from Pseudomonas aeruginosa==
		+	<StructureSection load='5tsy' size='340' side='right' caption='[[5tsy]], [[Resolution\|resolution]] 1.90Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5tsy]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5TSY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5TSY FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5tsy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5tsy OCA], [http://pdbe.org/5tsy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5tsy RCSB], [http://www.ebi.ac.uk/pdbsum/5tsy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5tsy ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Bacterial biofilms present a significant medical challenge because they are recalcitrant to current therapeutic regimes. A key component of biofilm formation in the opportunistic human pathogen Pseudomonas aeruginosa is the biosynthesis of the exopolysaccharides Pel and Psl, which are involved in the formation and maintenance of the structural biofilm scaffold and protection against antimicrobials and host defenses. Given that the glycoside hydrolases PelAh and PslGh encoded in the pel and psl biosynthetic operons, respectively, are utilized for in vivo exopolysaccharide processing, we reasoned that these would provide specificity to target P. aeruginosa biofilms. Evaluating these enzymes as potential therapeutics, we demonstrate that these glycoside hydrolases selectively target and degrade the exopolysaccharide component of the biofilm matrix. PelAh and PslGh inhibit biofilm formation over a 24-hour period with a half maximal effective concentration (EC50) of 69.3 +/- 1.2 and 4.1 +/- 1.1 nM, respectively, and are capable of disrupting preexisting biofilms in 1 hour with EC50 of 35.7 +/- 1.1 and 12.9 +/- 1.1 nM, respectively. This treatment was effective against clinical and environmental P. aeruginosa isolates and reduced biofilm biomass by 58 to 94%. These noncytotoxic enzymes potentiated antibiotics because the addition of either enzyme to a sublethal concentration of colistin reduced viable bacterial counts by 2.5 orders of magnitude when used either prophylactically or on established 24-hour biofilms. In addition, PelAh was able to increase neutrophil killing by ~50%. This work illustrates the feasibility and benefits of using bacterial exopolysaccharide biosynthetic glycoside hydrolases to develop novel antibiofilm therapeutics.

-	~~Authors: Baker~~, P., ~~Pfoh~~, R., ~~Howell~~, P.L.	+	Exopolysaccharide biosynthetic glycoside hydrolases can be utilized to disrupt and prevent Pseudomonas aeruginosa biofilms.,Baker P, Hill PJ, Snarr BD, Alnabelseya N, Pestrak MJ, Lee MJ, Jennings LK, Tam J, Melnyk RA, Parsek MR, Sheppard DC, Wozniak DJ, Howell PL Sci Adv. 2016 May 20;2(5):e1501632. doi: 10.1126/sciadv.1501632. eCollection 2016, May. PMID:27386527<ref>PMID:27386527</ref>

-	~~Description: Structure~~ of the ~~glycoside hydrolase domain~~ of ~~PelA variant E218A from Pseudomonas aeruginosa~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category~~: ~~Unreleased Structures]]~~	+	</div>
		+	<div class="pdbe-citations 5tsy" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Baker, P]]		[[Category: Baker, P]]
-	[[Category: Howell, P.L]]	+	[[Category: Howell, P L]]
	[[Category: Pfoh, R]]		[[Category: Pfoh, R]]
		+	[[Category: Glycoside hydrolase]]
		+	[[Category: Hydrolase]]

OCA at 13:27, 27 September 2017

OCA — Wed, 27 Sep 2017 13:27:35 GMT

←Older revision		Revision as of 13:27, 27 September 2017
Line 7:		Line 7:
	Description: Structure of the glycoside hydrolase domain of PelA variant E218A from Pseudomonas aeruginosa		Description: Structure of the glycoside hydrolase domain of PelA variant E218A from Pseudomonas aeruginosa
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Howell, P.L]]
	[[Category: Baker, P]]		[[Category: Baker, P]]
		+	[[Category: Howell, P.L]]
	[[Category: Pfoh, R]]		[[Category: Pfoh, R]]

OCA at 13:46, 9 March 2017

OCA — Thu, 09 Mar 2017 13:46:53 GMT

←Older revision		Revision as of 13:46, 9 March 2017
Line 7:		Line 7:
	Description: Structure of the glycoside hydrolase domain of PelA variant E218A from Pseudomonas aeruginosa		Description: Structure of the glycoside hydrolase domain of PelA variant E218A from Pseudomonas aeruginosa
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Baker, P]]
	[[Category: Howell, P.L]]		[[Category: Howell, P.L]]
		+	[[Category: Baker, P]]
	[[Category: Pfoh, R]]		[[Category: Pfoh, R]]

OCA: Protected "5tsy" [edit=sysop:move=sysop]

OCA — Fri, 09 Dec 2016 17:50:10 GMT

Protected "5tsy" [edit=sysop:move=sysop]

←Older revision

Revision as of 17:50, 9 December 2016

OCA: New page: '''Unreleased structure''' The entry 5tsy is ON HOLD until Paper Publication Authors: Baker, P., Pfoh, R., Howell, P.L. Description: Structure of the glycoside hydrolase domain of PelA...

OCA — Fri, 09 Dec 2016 17:50:09 GMT

New page: '''Unreleased structure''' The entry 5tsy is ON HOLD until Paper Publication Authors: Baker, P., Pfoh, R., Howell, P.L. Description: Structure of the glycoside hydrolase domain of PelA...

New page

'''Unreleased structure'''

The entry 5tsy is ON HOLD until Paper Publication

Authors: Baker, P., Pfoh, R., Howell, P.L.

Description: Structure of the glycoside hydrolase domain of PelA variant E218A from Pseudomonas aeruginosa
[[Category: Unreleased Structures]]
[[Category: Baker, P]]
[[Category: Howell, P.L]]
[[Category: Pfoh, R]]