5tv0 - Revision history

OCA at 13:13, 4 October 2023

OCA — Wed, 04 Oct 2023 13:13:02 GMT

←Older revision		Revision as of 13:13, 4 October 2023
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	<StructureSection load='5tv0' size='340' side='right'caption='[[5tv0]], [[Resolution\|resolution]] 1.65Å' scene=''>		<StructureSection load='5tv0' size='340' side='right'caption='[[5tv0]], [[Resolution\|resolution]] 1.65Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5tv0]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Syny3 Syny3~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5TV0 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5TV0 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5tv0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Synechocystis_sp._PCC_6803_substr._Kazusa Synechocystis sp. PCC 6803 substr. Kazusa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5TV0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5TV0 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat">~~<scene name='pdbligand=EQ3:(3R)~~-~~3-HYDROXY-BETA~~,~~BETA-CAROTEN-4-ONE'>EQ3</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.648Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat">~~[[5tuw\|5tuw]], [[5tux\|5tux]]~~<~~/td~~></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EQ3:(3R)-3-HYDROXY-BETA,BETA-CAROTEN-4-ONE'>EQ3</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
-	<~~tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">slr1963 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1111708 SYNY3])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5tv0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5tv0 OCA], [https://pdbe.org/5tv0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5tv0 RCSB], [https://www.ebi.ac.uk/pdbsum/5tv0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5tv0 ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5tv0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5tv0 OCA], [~~http~~://pdbe.org/5tv0 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5tv0 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5tv0 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5tv0 ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/OCP_SYNY3 OCP_SYNY3]] Acts as a photo-protectant. Essential for inhibiting white and blue-green light non-photochemical quenching (NPQ). Binding carotenoids improves OCP's intrinsic photoprotectant activity by broadening its absorption spectrum and facilitating the dissipation of absorbed energy.<ref>PMID:16531492</ref>	+	[https://www.uniprot.org/uniprot/OCP_SYNY3 OCP_SYNY3] Acts as a photo-protectant. Essential for inhibiting white and blue-green light non-photochemical quenching (NPQ). Binding carotenoids improves OCP's intrinsic photoprotectant activity by broadening its absorption spectrum and facilitating the dissipation of absorbed energy.<ref>PMID:16531492</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Syny3~~]]	+	[[Category: Synechocystis sp. PCC 6803 substr. Kazusa]]
-	[[Category: Bandara, S]]	+	[[Category: Bandara S]]
-	[[Category: Ren, Z]]	+	[[Category: Ren Z]]
-	[[Category: Yang, X]]	+	[[Category: Yang X]]
-	~~[[Category: Carotenoid binding protein]]~~	+
-	~~[[Category: Dynamic crystallography]]~~	+
-	~~[[Category: Photoprotection]]~~	+
-	~~[[Category: Photoreceptor~~]]	+

OCA at 16:59, 11 December 2019

OCA — Wed, 11 Dec 2019 16:59:50 GMT

←Older revision		Revision as of 16:59, 11 December 2019
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	==crystal structure and light induced structural changes in orange carotenoid protein bound with 3 'OH echinenone==		==crystal structure and light induced structural changes in orange carotenoid protein bound with 3 'OH echinenone==
-	<StructureSection load='5tv0' size='340' side='right' caption='[[5tv0]], [[Resolution\|resolution]] 1.65Å' scene=''>	+	<StructureSection load='5tv0' size='340' side='right'caption='[[5tv0]], [[Resolution\|resolution]] 1.65Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5tv0]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5TV0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5TV0 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5tv0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Syny3 Syny3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5TV0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5TV0 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EQ3:(3R)-3-HYDROXY-BETA,BETA-CAROTEN-4-ONE'>EQ3</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EQ3:(3R)-3-HYDROXY-BETA,BETA-CAROTEN-4-ONE'>EQ3</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5tuw\|5tuw]], [[5tux\|5tux]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5tuw\|5tuw]], [[5tux\|5tux]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">slr1963 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1111708 SYNY3])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5tv0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5tv0 OCA], [http://pdbe.org/5tv0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5tv0 RCSB], [http://www.ebi.ac.uk/pdbsum/5tv0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5tv0 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5tv0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5tv0 OCA], [http://pdbe.org/5tv0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5tv0 RCSB], [http://www.ebi.ac.uk/pdbsum/5tv0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5tv0 ProSAT]</span></td></tr>
	</table>		</table>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Syny3]]
	[[Category: Bandara, S]]		[[Category: Bandara, S]]
	[[Category: Ren, Z]]		[[Category: Ren, Z]]

OCA at 10:39, 3 August 2017

OCA — Thu, 03 Aug 2017 10:39:19 GMT

←Older revision		Revision as of 10:39, 3 August 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5tv0 is ~~ON HOLD~~	+	==crystal structure and light induced structural changes in orange carotenoid protein bound with 3 'OH echinenone==
		+	<StructureSection load='5tv0' size='340' side='right' caption='[[5tv0]], [[Resolution\|resolution]] 1.65Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5tv0]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5TV0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5TV0 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EQ3:(3R)-3-HYDROXY-BETA,BETA-CAROTEN-4-ONE'>EQ3</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5tuw\|5tuw]], [[5tux\|5tux]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5tv0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5tv0 OCA], [http://pdbe.org/5tv0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5tv0 RCSB], [http://www.ebi.ac.uk/pdbsum/5tv0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5tv0 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/OCP_SYNY3 OCP_SYNY3]] Acts as a photo-protectant. Essential for inhibiting white and blue-green light non-photochemical quenching (NPQ). Binding carotenoids improves OCP's intrinsic photoprotectant activity by broadening its absorption spectrum and facilitating the dissipation of absorbed energy.<ref>PMID:16531492</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Photoprotection is essential for efficient photosynthesis. Cyanobacteria have evolved a unique photoprotective mechanism mediated by a water-soluble carotenoid-based photoreceptor known as orange carotenoid protein (OCP). OCP undergoes large conformational changes in response to intense blue light, and the photoactivated OCP facilitates dissipation of excess energy via direct interaction with allophycocyanins at the phycobilisome core. However, the structural events leading up to the OCP photoactivation remain elusive at the molecular level. Here we present direct observations of light-induced structural changes in OCP captured by dynamic crystallography. Difference electron densities between the dark and illuminated states reveal widespread and concerted atomic motions that lead to altered protein-pigment interactions, displacement of secondary structures, and domain separation. Based on these crystallographic observations together with site-directed mutagenesis, we propose a molecular mechanism for OCP light perception, in which the photochemical property of a conjugated carbonyl group is exploited. We hypothesize that the OCP photoactivation starts with keto-enol tautomerization of the essential 4-keto group in the carotenoid, which disrupts the strong hydrogen bonds between the bent chromophore and the protein moiety. Subsequent structural changes trapped in the crystal lattice offer a high-resolution glimpse of the initial molecular events as OCP begins to transition from the orange-absorbing state to the active red-absorbing state.

-	~~Authors: Yang, X~~., Bandara, S., Ren, Z.	+	Photoactivation mechanism of a carotenoid-based photoreceptor.,Bandara S, Ren Z, Lu L, Zeng X, Shin H, Zhao KH, Yang X Proc Natl Acad Sci U S A. 2017 Jun 13;114(24):6286-6291. doi:, 10.1073/pnas.1700956114. Epub 2017 May 30. PMID:28559328<ref>PMID:28559328</ref>

-	~~Description: crystal structure and light induced structural changes in orange carotenoid protein bound with 3 'OH echinenone~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category: Unreleased Structures]]~~	+	</div>
-	~~[[Category~~: ~~Yang, X]]~~	+	<div class="pdbe-citations 5tv0" style="background-color:#fffaf0;"></div>
-	~~[[Category: Ren, Z]]~~	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Bandara, S]]		[[Category: Bandara, S]]
		+	[[Category: Ren, Z]]
		+	[[Category: Yang, X]]
		+	[[Category: Carotenoid binding protein]]
		+	[[Category: Dynamic crystallography]]
		+	[[Category: Photoprotection]]
		+	[[Category: Photoreceptor]]

OCA at 10:33, 24 May 2017

OCA — Wed, 24 May 2017 10:33:38 GMT

←Older revision		Revision as of 10:33, 24 May 2017
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5tv0 is ON HOLD ~~until Paper Publication~~	+	The entry 5tv0 is ON HOLD

	Authors: Yang, X., Bandara, S., Ren, Z.		Authors: Yang, X., Bandara, S., Ren, Z.

OCA at 13:48, 9 March 2017

OCA — Thu, 09 Mar 2017 13:48:54 GMT

←Older revision		Revision as of 13:48, 9 March 2017
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	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Yang, X]]		[[Category: Yang, X]]
-	[[Category: Bandara, S]]
	[[Category: Ren, Z]]		[[Category: Ren, Z]]
		+	[[Category: Bandara, S]]

OCA at 14:48, 2 January 2017

OCA — Mon, 02 Jan 2017 14:48:27 GMT

←Older revision		Revision as of 14:48, 2 January 2017
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5tv0 is ON HOLD	+	The entry 5tv0 is ON HOLD until Paper Publication

	Authors: Yang, X., Bandara, S., Ren, Z.		Authors: Yang, X., Bandara, S., Ren, Z.

OCA: Protected "5tv0" [edit=sysop:move=sysop]

OCA — Fri, 09 Dec 2016 17:55:20 GMT

Protected "5tv0" [edit=sysop:move=sysop]

←Older revision

Revision as of 17:55, 9 December 2016

OCA: New page: '''Unreleased structure''' The entry 5tv0 is ON HOLD Authors: Yang, X., Bandara, S., Ren, Z. Description: crystal structure and light induced structural changes in orange carotenoid pr...

OCA — Fri, 09 Dec 2016 17:55:19 GMT

New page: '''Unreleased structure''' The entry 5tv0 is ON HOLD Authors: Yang, X., Bandara, S., Ren, Z. Description: crystal structure and light induced structural changes in orange carotenoid pr...

New page

'''Unreleased structure'''

The entry 5tv0 is ON HOLD

Authors: Yang, X., Bandara, S., Ren, Z.

Description: crystal structure and light induced structural changes in orange carotenoid protein bound with 3 'OH echinenone
[[Category: Unreleased Structures]]
[[Category: Yang, X]]
[[Category: Bandara, S]]
[[Category: Ren, Z]]