5tvu - Revision history

OCA at 13:13, 4 October 2023

OCA — Wed, 04 Oct 2023 13:13:53 GMT

←Older revision		Revision as of 13:13, 4 October 2023
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	<StructureSection load='5tvu' size='340' side='right'caption='[[5tvu]], [[Resolution\|resolution]] 3.50Å' scene=''>		<StructureSection load='5tvu' size='340' side='right'caption='[[5tvu]], [[Resolution\|resolution]] 3.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5tvu]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Brachidanio_rerio Brachidanio~~ rerio]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5TVU OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5TVU FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5tvu]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Danio_rerio Danio rerio]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5TVU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5TVU FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat"~~><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.5Å</td></tr>
-	~~<tr~~ id~~='related'><td class~~="~~sblockLbl~~">~~<b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5tvw\|5tvw]]~~, [[~~5tvx~~\|~~5tvx~~]]</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene></td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~trap1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=5~~&id=7955 Brachidanio rerio])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5tvu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5tvu OCA], [https://pdbe.org/5tvu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5tvu RCSB], [https://www.ebi.ac.uk/pdbsum/5tvu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5tvu ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5tvu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5tvu OCA], [~~http~~://pdbe.org/5tvu PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5tvu RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5tvu PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5tvu ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/F1Q9X9_DANRE F1Q9X9_DANRE]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Brachidanio~~ rerio]]	+	[[Category: Danio rerio]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Agard~~, D A~~]]	+	[[Category: Agard DA]]
-	[[Category: Betegon, M]]	+	[[Category: Betegon M]]
-	[[Category: Elnatan, D]]	+	[[Category: Elnatan D]]
-	~~[[Category: Atp]]~~	+
-	~~[[Category: Chaperone]]~~	+
-	~~[[Category: Hsp90]]~~	+
-	~~[[Category: PSI, Protein structure initiative]]~~	+
-	~~[[Category: Structural genomic]]~~	+
-	~~[[Category: Trap1~~]]	+

OCA at 13:20, 10 May 2019

OCA — Fri, 10 May 2019 13:20:09 GMT

←Older revision		Revision as of 13:20, 10 May 2019
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	==Crystal structure of mitochondrial Hsp90 (TRAP1) with ATP in absence of Mg==		==Crystal structure of mitochondrial Hsp90 (TRAP1) with ATP in absence of Mg==
-	<StructureSection load='5tvu' size='340' side='right' caption='[[5tvu]], [[Resolution\|resolution]] 3.50Å' scene=''>	+	<StructureSection load='5tvu' size='340' side='right'caption='[[5tvu]], [[Resolution\|resolution]] 3.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5tvu]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5TVU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5TVU FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5tvu]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Brachidanio_rerio Brachidanio rerio]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5TVU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5TVU FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5tvw\|5tvw]], [[5tvx\|5tvx]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5tvw\|5tvw]], [[5tvx\|5tvx]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">trap1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7955 Brachidanio rerio])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5tvu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5tvu OCA], [http://pdbe.org/5tvu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5tvu RCSB], [http://www.ebi.ac.uk/pdbsum/5tvu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5tvu ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5tvu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5tvu OCA], [http://pdbe.org/5tvu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5tvu RCSB], [http://www.ebi.ac.uk/pdbsum/5tvu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5tvu ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Hsp90 is a homodimeric ATP-dependent molecular chaperone that remodels its substrate 'client' proteins, facilitating their folding and activating them for biological function. Despite decades of research, the mechanism connecting ATP hydrolysis and chaperone function remains elusive. Particularly puzzling has been the apparent lack of cooperativity in hydrolysis of the ATP in each protomer. A crystal structure of the mitochondrial Hsp90, TRAP1, revealed that the catalytically active state is closed in a highly strained asymmetric conformation. This asymmetry, unobserved in other Hsp90 homologs, is due to buckling of one of the protomers and is most pronounced at the broadly conserved client-binding region. Here, we show that rather than being cooperative or independent, ATP hydrolysis on the two protomers is sequential and deterministic. Moreover, dimer asymmetry sets up differential hydrolysis rates for each protomer, such that the buckled conformation favors ATP hydrolysis. Remarkably, after the first hydrolysis, the dimer undergoes a flip in the asymmetry while remaining in a closed state for the second hydrolysis. From these results, we propose a model where direct coupling of ATP hydrolysis and conformational flipping rearranges client-binding sites, providing a paradigm of how energy from ATP hydrolysis can be used for client remodeling.
		+
		+	Symmetry broken and rebroken during the ATP hydrolysis cycle of the mitochondrial Hsp90 TRAP1.,Elnatan D, Betegon M, Liu Y, Ramelot T, Kennedy MA, Agard DA Elife. 2017 Jul 25;6. pii: e25235. doi: 10.7554/eLife.25235. PMID:28742020<ref>PMID:28742020</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5tvu" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Brachidanio rerio]]
		+	[[Category: Large Structures]]
	[[Category: Agard, D A]]		[[Category: Agard, D A]]
	[[Category: Betegon, M]]		[[Category: Betegon, M]]

OCA at 06:21, 14 March 2018

OCA — Wed, 14 Mar 2018 06:21:52 GMT

←Older revision		Revision as of 06:21, 14 March 2018
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-	'''Unreleased structure'''

-	~~The entry~~ 5tvu is ~~ON HOLD~~	+	==Crystal structure of mitochondrial Hsp90 (TRAP1) with ATP in absence of Mg==
-		+	<StructureSection load='5tvu' size='340' side='right' caption='[[5tvu]], [[Resolution\|resolution]] 3.50Å' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5tvu]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5TVU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5TVU FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5tvw\|5tvw]], [[5tvx\|5tvx]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5tvu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5tvu OCA], [http://pdbe.org/5tvu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5tvu RCSB], [http://www.ebi.ac.uk/pdbsum/5tvu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5tvu ProSAT]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Agard, D A]]
		+	[[Category: Betegon, M]]
		+	[[Category: Elnatan, D]]
		+	[[Category: Atp]]
		+	[[Category: Chaperone]]
		+	[[Category: Hsp90]]
		+	[[Category: PSI, Protein structure initiative]]
		+	[[Category: Structural genomic]]
		+	[[Category: Trap1]]

OCA: Protected "5tvu" [edit=sysop:move=sysop]

OCA — Fri, 09 Dec 2016 17:57:24 GMT

Protected "5tvu" [edit=sysop:move=sysop]

←Older revision

Revision as of 17:57, 9 December 2016

OCA: New page: '''Unreleased structure''' The entry 5tvu is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Fri, 09 Dec 2016 17:57:23 GMT

New page: '''Unreleased structure''' The entry 5tvu is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 5tvu is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]