5u32 - Revision history

OCA at 14:24, 6 March 2024

OCA — Wed, 06 Mar 2024 14:24:50 GMT

←Older revision		Revision as of 14:24, 6 March 2024
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	<StructureSection load='5u32' size='340' side='right'caption='[[5u32]], [[Resolution\|resolution]] 2.19Å' scene=''>		<StructureSection load='5u32' size='340' side='right'caption='[[5u32]], [[Resolution\|resolution]] 2.19Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5u32]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Canal Canal~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5U32 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5U32 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5u32]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Candida_albicans_SC5314 Candida albicans SC5314]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5U32 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5U32 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat">~~<scene name='pdbligand=GDP:GUANOSINE~~-~~5-DIPHOSPHATE'>GDP</scene>~~, ~~<scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.191Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>LIG1, RLG1, TRL1, CAALFM_C702060WA, CaJ7.0238, CaO19.13864, CaO19.6511 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~237561 CANAL])</td~~><~~/tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b~~></td><~~td class~~=~~"sblockDat"><span class~~=~~'plainlinks~~'>~~[http://en.wikipedia.org/wiki/RNA_ligase_(ATP) RNA ligase (ATP)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.5.1.3 6.5.1.3]~~ </~~span~~></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5u32 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5u32 OCA], [https://pdbe.org/5u32 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5u32 RCSB], [https://www.ebi.ac.uk/pdbsum/5u32 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5u32 ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5u32 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5u32 OCA], [~~http~~://pdbe.org/5u32 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5u32 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5u32 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5u32 ProSAT]</span></td></tr>	+
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/TRNL_CANAL TRNL_CANAL] One of the two proteins required for the splicing of precursor tRNA molecules containing introns. The ligation activity requires three enzymatic activities: phosphorylation of the 5' terminus of the 3' half-tRNA in the presence of ATP, opening of the 2'3'-cyclic phosphodiester bond of the 5' half-tRNA leaving a 2'-phosphomonoester and ligation of the two tRNA halves in an ATP-dependent reaction.
-	Fungal tRNA ligase (Trl1) is an essential enzyme that repairs RNA breaks with 2',3'-cyclic-PO4 and 5'-OH ends inflicted during tRNA splicing and non-canonical mRNA splicing in the fungal unfolded protein response. ~~Trl1 is composed~~ of ~~C-terminal cyclic phosphodiesterase and central polynucleotide kinase domains that heal~~ the ~~broken ends to generate the 3'-OH,2'-PO4 and 5'-PO4 termini~~ required for sealing by an N-terminal ligase domain. Trl1 enzymes are found in all human fungal pathogens and are promising targets for antifungal drug discovery because their domain compositions and biochemical mechanisms are unique compared to the ~~mammalian RtcB-type tRNA~~ splicing ~~enzyme~~. ~~A distinctive feature~~ of ~~Trl1 is its preferential use~~ of ~~GTP as phosphate donor for~~ the ~~RNA kinase reaction. Here we report~~ the ~~2.2 A crystal structure~~ of ~~the kinase domain~~ of ~~Trl1 from~~ the ~~fungal pathogen Candida albicans with GDP and Mg2+ in the active site. The P~~-~~loop phosphotransferase fold~~ of the ~~kinase is embellished by~~ a ~~unique~~ 'G-~~loop' element that accounts for guanine nucleotide specificity. Mutations~~ of ~~amino acids that contact~~ the ~~guanine nucleobase efface kinase activity~~ in ~~vitro and Trl1 function in vivo. Our findings fortify the case for the Trl1 kinase as~~ an ~~antifungal target~~.	+
-		+
-	Structural basis for the GTP specificity of the RNA kinase domain of fungal tRNA ligase.,Remus BS, Goldgur Y, Shuman S Nucleic Acids Res. 2017 Nov 20. pii: 4641907. doi: 10.1093/nar/gkx1159. PMID:29165709<ref>PMID:29165709</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 5u32" style="background-color:#fffaf0;"></div>~~	+
-	~~== References ==~~	+
-	~~<references/>~~	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Canal~~]]	+	[[Category: Candida albicans SC5314]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Goldgur, Y]]	+	[[Category: Goldgur Y]]
-	[[Category: Remus~~, B S~~]]	+	[[Category: Remus BS]]
-	[[Category: Shuman, S]]	+	[[Category: Shuman S]]
-	~~[[Category: Polynucleotide kinase]]~~	+
-	~~[[Category: Rna ligase]]~~	+
-	~~[[Category: Rna repair]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 09:10, 1 January 2020

OCA — Wed, 01 Jan 2020 09:10:23 GMT

←Older revision		Revision as of 09:10, 1 January 2020
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	==Crystal Structure of Fungal RNA Kinase==		==Crystal Structure of Fungal RNA Kinase==
-	<StructureSection load='5u32' size='340' side='right' caption='[[5u32]], [[Resolution\|resolution]] 2.19Å' scene=''>	+	<StructureSection load='5u32' size='340' side='right'caption='[[5u32]], [[Resolution\|resolution]] 2.19Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5u32]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Canal Canal]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5U32 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5U32 FirstGlance]. <br>		<table><tr><td colspan='2'>[[5u32]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Canal Canal]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5U32 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5U32 FirstGlance]. <br>
Line 23:		Line 23:
	</StructureSection>		</StructureSection>
	[[Category: Canal]]		[[Category: Canal]]
		+	[[Category: Large Structures]]
	[[Category: Goldgur, Y]]		[[Category: Goldgur, Y]]
	[[Category: Remus, B S]]		[[Category: Remus, B S]]

OCA at 07:31, 13 December 2017

OCA — Wed, 13 Dec 2017 07:31:34 GMT

←Older revision		Revision as of 07:31, 13 December 2017
Line 9:		Line 9:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5u32 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5u32 OCA], [http://pdbe.org/5u32 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5u32 RCSB], [http://www.ebi.ac.uk/pdbsum/5u32 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5u32 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5u32 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5u32 OCA], [http://pdbe.org/5u32 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5u32 RCSB], [http://www.ebi.ac.uk/pdbsum/5u32 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5u32 ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Fungal tRNA ligase (Trl1) is an essential enzyme that repairs RNA breaks with 2',3'-cyclic-PO4 and 5'-OH ends inflicted during tRNA splicing and non-canonical mRNA splicing in the fungal unfolded protein response. Trl1 is composed of C-terminal cyclic phosphodiesterase and central polynucleotide kinase domains that heal the broken ends to generate the 3'-OH,2'-PO4 and 5'-PO4 termini required for sealing by an N-terminal ligase domain. Trl1 enzymes are found in all human fungal pathogens and are promising targets for antifungal drug discovery because their domain compositions and biochemical mechanisms are unique compared to the mammalian RtcB-type tRNA splicing enzyme. A distinctive feature of Trl1 is its preferential use of GTP as phosphate donor for the RNA kinase reaction. Here we report the 2.2 A crystal structure of the kinase domain of Trl1 from the fungal pathogen Candida albicans with GDP and Mg2+ in the active site. The P-loop phosphotransferase fold of the kinase is embellished by a unique 'G-loop' element that accounts for guanine nucleotide specificity. Mutations of amino acids that contact the guanine nucleobase efface kinase activity in vitro and Trl1 function in vivo. Our findings fortify the case for the Trl1 kinase as an antifungal target.
		+
		+	Structural basis for the GTP specificity of the RNA kinase domain of fungal tRNA ligase.,Remus BS, Goldgur Y, Shuman S Nucleic Acids Res. 2017 Nov 20. pii: 4641907. doi: 10.1093/nar/gkx1159. PMID:29165709<ref>PMID:29165709</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5u32" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 10:20, 22 November 2017

OCA — Wed, 22 Nov 2017 10:20:25 GMT

←Older revision		Revision as of 10:20, 22 November 2017
Line 3:		Line 3:
	<StructureSection load='5u32' size='340' side='right' caption='[[5u32]], [[Resolution\|resolution]] 2.19Å' scene=''>		<StructureSection load='5u32' size='340' side='right' caption='[[5u32]], [[Resolution\|resolution]] 2.19Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5u32]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5U32 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5U32 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5u32]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Canal Canal]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5U32 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5U32 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">LIG1, RLG1, TRL1, CAALFM_C702060WA, CaJ7.0238, CaO19.13864, CaO19.6511 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=237561 CANAL])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/RNA_ligase_(ATP) RNA ligase (ATP)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.5.1.3 6.5.1.3] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/RNA_ligase_(ATP) RNA ligase (ATP)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.5.1.3 6.5.1.3] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5u32 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5u32 OCA], [http://pdbe.org/5u32 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5u32 RCSB], [http://www.ebi.ac.uk/pdbsum/5u32 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5u32 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5u32 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5u32 OCA], [http://pdbe.org/5u32 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5u32 RCSB], [http://www.ebi.ac.uk/pdbsum/5u32 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5u32 ProSAT]</span></td></tr>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Canal]]
	[[Category: Goldgur, Y]]		[[Category: Goldgur, Y]]
	[[Category: Remus, B S]]		[[Category: Remus, B S]]

OCA at 07:31, 15 November 2017

OCA — Wed, 15 Nov 2017 07:31:27 GMT

←Older revision		Revision as of 07:31, 15 November 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5u32 is ~~ON HOLD until Paper Publication~~	+	==Crystal Structure of Fungal RNA Kinase==
-		+	<StructureSection load='5u32' size='340' side='right' caption='[[5u32]], [[Resolution\|resolution]] 2.19Å' scene=''>
-	~~Authors~~: ~~Shuman~~, S., ~~Goldgur~~, Y., ~~Remus~~, B.S.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5u32]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5U32 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5U32 FirstGlance]. <br>
-	~~Description~~: ~~Crystal Structure of Fungal RNA Kinase~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/RNA_ligase_(ATP) RNA ligase (ATP)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.5.1.3 6.5.1.3] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5u32 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5u32 OCA], [http://pdbe.org/5u32 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5u32 RCSB], [http://www.ebi.ac.uk/pdbsum/5u32 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5u32 ProSAT]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Goldgur, Y]]
		+	[[Category: Remus, B S]]
	[[Category: Shuman, S]]		[[Category: Shuman, S]]
-	[[Category: ~~Remus, B.S~~]]	+	[[Category: Polynucleotide kinase]]
-	[[Category: ~~Goldgur, Y~~]]	+	[[Category: Rna ligase]]
		+	[[Category: Rna repair]]
		+	[[Category: Transferase]]

OCA at 07:59, 4 October 2017

OCA — Wed, 04 Oct 2017 07:59:03 GMT

←Older revision		Revision as of 07:59, 4 October 2017
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5u32 is ON HOLD	+	The entry 5u32 is ON HOLD until Paper Publication

	Authors: Shuman, S., Goldgur, Y., Remus, B.S.		Authors: Shuman, S., Goldgur, Y., Remus, B.S.
Line 7:		Line 7:
	Description: Crystal Structure of Fungal RNA Kinase		Description: Crystal Structure of Fungal RNA Kinase
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Goldgur, Y]]
	[[Category: Shuman, S]]		[[Category: Shuman, S]]
	[[Category: Remus, B.S]]		[[Category: Remus, B.S]]
		+	[[Category: Goldgur, Y]]

OCA at 13:32, 27 September 2017

OCA — Wed, 27 Sep 2017 13:32:19 GMT

←Older revision		Revision as of 13:32, 27 September 2017
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5u32 is ON HOLD ~~until Paper Publication~~	+	The entry 5u32 is ON HOLD

	Authors: Shuman, S., Goldgur, Y., Remus, B.S.		Authors: Shuman, S., Goldgur, Y., Remus, B.S.

OCA at 14:21, 18 January 2017

OCA — Wed, 18 Jan 2017 14:21:01 GMT

←Older revision		Revision as of 14:21, 18 January 2017
Line 3:		Line 3:
	The entry 5u32 is ON HOLD until Paper Publication		The entry 5u32 is ON HOLD until Paper Publication

-	Authors: Shuman, S., Goldgur , Y., Remus, B.S.	+	Authors: Shuman, S., Goldgur, Y., Remus, B.S.

	Description: Crystal Structure of Fungal RNA Kinase		Description: Crystal Structure of Fungal RNA Kinase
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Goldgur , Y]]	+	[[Category: Goldgur, Y]]
	[[Category: Shuman, S]]		[[Category: Shuman, S]]
	[[Category: Remus, B.S]]		[[Category: Remus, B.S]]

OCA at 17:36, 15 December 2016

OCA — Thu, 15 Dec 2016 17:36:07 GMT

←Older revision		Revision as of 17:36, 15 December 2016
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5u32 is ON HOLD	+	The entry 5u32 is ON HOLD until Paper Publication

	Authors: Shuman, S., Goldgur , Y., Remus, B.S.		Authors: Shuman, S., Goldgur , Y., Remus, B.S.

-	Description: Fungal RNA Kinase	+	Description: Crystal Structure of Fungal RNA Kinase
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Goldgur , Y]]		[[Category: Goldgur , Y]]
	[[Category: Shuman, S]]		[[Category: Shuman, S]]
	[[Category: Remus, B.S]]		[[Category: Remus, B.S]]

OCA: Protected "5u32" [edit=sysop:move=sysop]

OCA — Fri, 09 Dec 2016 18:15:00 GMT

Protected "5u32" [edit=sysop:move=sysop]

←Older revision

Revision as of 18:15, 9 December 2016