5u70 - Revision history

OCA at 15:45, 6 March 2024

OCA — Wed, 06 Mar 2024 15:45:17 GMT

←Older revision		Revision as of 15:45, 6 March 2024
Line 3:		Line 3:
	<SX load='5u70' size='340' side='right' viewer='molstar' caption='[[5u70]], [[Resolution\|resolution]] 3.70Å' scene=''>		<SX load='5u70' size='340' side='right' viewer='molstar' caption='[[5u70]], [[Resolution\|resolution]] 3.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5u70]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Chick Chick~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5U70 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5U70 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5u70]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5U70 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5U70 FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~>[[5u76\|5u76]]</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 3.7Å</td></tr>
-	~~<tr~~ id~~='gene'><td class~~="~~sblockLbl~~">~~<b>~~[[~~Gene~~\|~~Gene:~~]]~~</b></td><td class="sblockDat">KCNT1, SLACK ([http://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=9031 CHICK])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5u70 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5u70 OCA], [https://pdbe.org/5u70 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5u70 RCSB], [https://www.ebi.ac.uk/pdbsum/5u70 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5u70 ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5u70 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5u70 OCA], [~~http~~://pdbe.org/5u70 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5u70 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5u70 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5u70 ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/KCNT1_CHICK KCNT1_CHICK]] Generates outwardly rectifying currents that are suppressed by elevation of intracellular calcium.	+	[https://www.uniprot.org/uniprot/KCNT1_CHICK KCNT1_CHICK] Generates outwardly rectifying currents that are suppressed by elevation of intracellular calcium.
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	The stable structural conformations that occur along the complete reaction coordinate for ion channel opening have never been observed. In this study, we describe the equilibrium ensemble of structures of Slo2.2, a neuronal Na+-activated K+ channel, as a function of the Na+ concentration. We find that Slo2.2 exists in multiple closed conformations whose relative occupancies are independent of Na+ concentration. An open conformation emerges from an ensemble of closed conformations in a highly Na+-dependent manner, without evidence of Na+-dependent intermediates. In other words, channel opening is a highly concerted, switch-like process. The midpoint of the structural titration matches that of the functional titration. A maximum open conformation probability approaching 1.0 and maximum functional open probability approaching 0.7 imply that, within the class of open channels, there is a subclass that is not permeable to ions.	+
-		+
-	Structural Titration of Slo2.2, a Na+-Dependent K+ Channel.,Hite RK, MacKinnon R Cell. 2017 Jan 26;168(3):390-399.e11. doi: 10.1016/j.cell.2016.12.030. Epub 2017 , Jan 19. PMID:28111072<ref>PMID:28111072</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 5u70" style="background-color:#fffaf0;"></div>~~	+

	==See Also==		==See Also==
	*[[Potassium channel 3D structures\|Potassium channel 3D structures]]		*[[Potassium channel 3D structures\|Potassium channel 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</SX>		</SX>
-	[[Category: ~~Chick~~]]	+	[[Category: Gallus gallus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Hite~~, R K~~]]	+	[[Category: Hite RK]]
-	[[Category: MacKinnon, R]]	+	[[Category: MacKinnon R]]
-	~~[[Category: Ion channel]]~~	+
-	~~[[Category: Potassium channel]]~~	+
-	~~[[Category: Transport protein~~]]	+

OCA at 21:51, 10 April 2020

OCA — Fri, 10 Apr 2020 21:51:53 GMT

←Older revision		Revision as of 21:51, 10 April 2020
Line 3:		Line 3:
	<SX load='5u70' size='340' side='right' viewer='molstar' caption='[[5u70]], [[Resolution\|resolution]] 3.70Å' scene=''>		<SX load='5u70' size='340' side='right' viewer='molstar' caption='[[5u70]], [[Resolution\|resolution]] 3.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5u70]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Chick Chick]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5U70 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5U70 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5u70]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Chick Chick]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5U70 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5U70 FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5u76\|5u76]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5u76\|5u76]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">KCNT1, SLACK ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9031 CHICK])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">KCNT1, SLACK ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9031 CHICK])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5u70 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5u70 OCA], [http://pdbe.org/5u70 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5u70 RCSB], [http://www.ebi.ac.uk/pdbsum/5u70 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5u70 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5u70 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5u70 OCA], [http://pdbe.org/5u70 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5u70 RCSB], [http://www.ebi.ac.uk/pdbsum/5u70 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5u70 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 19:41, 6 March 2020

OCA — Fri, 06 Mar 2020 19:41:46 GMT

←Older revision		Revision as of 19:41, 6 March 2020
Line 1:		Line 1:

	==Chicken Slo2.2 in an open conformation vitrified in the presence of 300 mM NaCl==		==Chicken Slo2.2 in an open conformation vitrified in the presence of 300 mM NaCl==
-	<~~StructureSection~~ load='5u70' size='340' side='right'caption='[[5u70]], [[Resolution\|resolution]] 3.70Å' scene=''>	+	<SX load='5u70' size='340' side='right' viewer='molstar' caption='[[5u70]], [[Resolution\|resolution]] 3.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5u70]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Chick Chick]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5U70 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5U70 FirstGlance]. <br>		<table><tr><td colspan='2'>[[5u70]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Chick Chick]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5U70 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5U70 FirstGlance]. <br>
Line 21:		Line 21:

	==See Also==		==See Also==
-	*[[Potassium ~~Channel~~\|Potassium ~~Channel~~]]	+	*[[Potassium channel 3D structures\|Potassium channel 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
-	</~~StructureSection~~>	+	</SX>
	[[Category: Chick]]		[[Category: Chick]]
	[[Category: Large Structures]]		[[Category: Large Structures]]

OCA at 16:43, 20 November 2019

OCA — Wed, 20 Nov 2019 16:43:12 GMT

←Older revision		Revision as of 16:43, 20 November 2019
Line 1:		Line 1:

	==Chicken Slo2.2 in an open conformation vitrified in the presence of 300 mM NaCl==		==Chicken Slo2.2 in an open conformation vitrified in the presence of 300 mM NaCl==
-	<StructureSection load='5u70' size='340' side='right' caption='[[5u70]], [[Resolution\|resolution]] 3.70Å' scene=''>	+	<StructureSection load='5u70' size='340' side='right'caption='[[5u70]], [[Resolution\|resolution]] 3.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5u70]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Chick Chick]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5U70 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5U70 FirstGlance]. <br>		<table><tr><td colspan='2'>[[5u70]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Chick Chick]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5U70 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5U70 FirstGlance]. <br>
Line 27:		Line 27:
	</StructureSection>		</StructureSection>
	[[Category: Chick]]		[[Category: Chick]]
		+	[[Category: Large Structures]]
	[[Category: Hite, R K]]		[[Category: Hite, R K]]
	[[Category: MacKinnon, R]]		[[Category: MacKinnon, R]]

OCA at 09:09, 18 July 2018

OCA — Wed, 18 Jul 2018 09:09:56 GMT

←Older revision		Revision as of 09:09, 18 July 2018
Line 19:		Line 19:
	</div>		</div>
	<div class="pdbe-citations 5u70" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5u70" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Potassium Channel\|Potassium Channel]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 06:07, 16 November 2017

OCA — Thu, 16 Nov 2017 06:07:46 GMT

←Older revision		Revision as of 06:07, 16 November 2017
Line 3:		Line 3:
	<StructureSection load='5u70' size='340' side='right' caption='[[5u70]], [[Resolution\|resolution]] 3.70Å' scene=''>		<StructureSection load='5u70' size='340' side='right' caption='[[5u70]], [[Resolution\|resolution]] 3.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5u70]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5U70 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5U70 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5u70]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Chick Chick]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5U70 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5U70 FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5u76\|5u76]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5u76\|5u76]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">KCNT1, SLACK ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9031 CHICK])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5u70 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5u70 OCA], [http://pdbe.org/5u70 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5u70 RCSB], [http://www.ebi.ac.uk/pdbsum/5u70 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5u70 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5u70 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5u70 OCA], [http://pdbe.org/5u70 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5u70 RCSB], [http://www.ebi.ac.uk/pdbsum/5u70 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5u70 ProSAT]</span></td></tr>
	</table>		</table>
Line 22:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Chick]]
	[[Category: Hite, R K]]		[[Category: Hite, R K]]
	[[Category: MacKinnon, R]]		[[Category: MacKinnon, R]]

OCA at 09:29, 10 March 2017

OCA — Fri, 10 Mar 2017 09:29:46 GMT

←Older revision		Revision as of 09:29, 10 March 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5u70 is ~~ON HOLD~~	+	==Chicken Slo2.2 in an open conformation vitrified in the presence of 300 mM NaCl==
		+	<StructureSection load='5u70' size='340' side='right' caption='[[5u70]], [[Resolution\|resolution]] 3.70Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5u70]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5U70 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5U70 FirstGlance]. <br>
		+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5u76\|5u76]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5u70 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5u70 OCA], [http://pdbe.org/5u70 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5u70 RCSB], [http://www.ebi.ac.uk/pdbsum/5u70 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5u70 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/KCNT1_CHICK KCNT1_CHICK]] Generates outwardly rectifying currents that are suppressed by elevation of intracellular calcium.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The stable structural conformations that occur along the complete reaction coordinate for ion channel opening have never been observed. In this study, we describe the equilibrium ensemble of structures of Slo2.2, a neuronal Na+-activated K+ channel, as a function of the Na+ concentration. We find that Slo2.2 exists in multiple closed conformations whose relative occupancies are independent of Na+ concentration. An open conformation emerges from an ensemble of closed conformations in a highly Na+-dependent manner, without evidence of Na+-dependent intermediates. In other words, channel opening is a highly concerted, switch-like process. The midpoint of the structural titration matches that of the functional titration. A maximum open conformation probability approaching 1.0 and maximum functional open probability approaching 0.7 imply that, within the class of open channels, there is a subclass that is not permeable to ions.

-	~~Authors: Hite, R~~.K., ~~MacKinnon~~, R.	+	Structural Titration of Slo2.2, a Na+-Dependent K+ Channel.,Hite RK, MacKinnon R Cell. 2017 Jan 26;168(3):390-399.e11. doi: 10.1016/j.cell.2016.12.030. Epub 2017 , Jan 19. PMID:28111072<ref>PMID:28111072</ref>

-	~~Description: Chicken Slo2.2 in an open conformation vitrified in~~ the ~~presence~~ of ~~300 mM NaCl~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
-	[[Category: ~~Mackinnon~~, R]]	+	<div class="pdbe-citations 5u70" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Hite, R.K~~]]	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Hite, R K]]
		+	[[Category: MacKinnon, R]]
		+	[[Category: Ion channel]]
		+	[[Category: Potassium channel]]
		+	[[Category: Transport protein]]

OCA at 15:08, 2 January 2017

OCA — Mon, 02 Jan 2017 15:08:25 GMT

←Older revision		Revision as of 15:08, 2 January 2017
Line 3:		Line 3:
	The entry 5u70 is ON HOLD		The entry 5u70 is ON HOLD

-	Authors: ~~Richard~~ K. ~~Hite~~, ~~Roderick~~ MacKinnon	+	Authors: Hite, R.K., MacKinnon, R.

	Description: Chicken Slo2.2 in an open conformation vitrified in the presence of 300 mM NaCl		Description: Chicken Slo2.2 in an open conformation vitrified in the presence of 300 mM NaCl
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: ~~Richard K.~~ Hite, ~~Roderick Mackinnon~~]]	+	[[Category: Mackinnon, R]]
		+	[[Category: Hite, R.K]]

OCA: Protected "5u70" [edit=sysop:move=sysop]

OCA — Thu, 22 Dec 2016 20:28:55 GMT

Protected "5u70" [edit=sysop:move=sysop]

←Older revision

Revision as of 20:28, 22 December 2016

OCA: New page: '''Unreleased structure''' The entry 5u70 is ON HOLD Authors: Richard K. Hite, Roderick MacKinnon Description: Chicken Slo2.2 in an open conformation vitrified in the presence of 300 m...

OCA — Thu, 22 Dec 2016 20:28:54 GMT

New page: '''Unreleased structure''' The entry 5u70 is ON HOLD Authors: Richard K. Hite, Roderick MacKinnon Description: Chicken Slo2.2 in an open conformation vitrified in the presence of 300 m...

New page

'''Unreleased structure'''

The entry 5u70 is ON HOLD

Authors: Richard K. Hite, Roderick MacKinnon

Description: Chicken Slo2.2 in an open conformation vitrified in the presence of 300 mM NaCl
[[Category: Unreleased Structures]]
[[Category: Richard K. Hite, Roderick Mackinnon]]