5u7f - Revision history

OCA at 07:03, 3 April 2024

OCA — Wed, 03 Apr 2024 07:03:09 GMT

←Older revision		Revision as of 07:03, 3 April 2024
Line 3:		Line 3:
	<StructureSection load='5u7f' size='340' side='right'caption='[[5u7f]], [[Resolution\|resolution]] 1.79Å' scene=''>		<StructureSection load='5u7f' size='340' side='right'caption='[[5u7f]], [[Resolution\|resolution]] 1.79Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5u7f]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Eco57 Eco57~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5U7F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5U7F FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5u7f]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_O157:H7 Escherichia coli O157:H7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5U7F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5U7F FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>~~, ~~<scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.789Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>~~[[~~Related_structure~~\|~~Related:~~]]~~</b></td><td class="sblockDat"><div style='overflow: auto~~; ~~max-height: 3em;'>[[5u7e\|5u7e]], [[5u7h\|5u7h]]</div>~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>nudB, Z2917, ECs2575 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~83334 ECO57])</td~~><~~/tr>~~	+
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b~~></td><~~td class~~=~~"sblockDat"><span class~~=~~'plainlinks~~'>[https://en.wikipedia.org/wiki/Dihydroneopterin_triphosphate_diphosphatase Dihydroneopterin triphosphate diphosphatase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.67 3.6.1.67] </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5u7f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5u7f OCA], [https://pdbe.org/5u7f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5u7f RCSB], [https://www.ebi.ac.uk/pdbsum/5u7f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5u7f ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5u7f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5u7f OCA], [https://pdbe.org/5u7f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5u7f RCSB], [https://www.ebi.ac.uk/pdbsum/5u7f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5u7f ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/~~NUDB_ECO57 NUDB_ECO57]~~] Catalyzes the hydrolysis of dihydroneopterin triphosphate to dihydroneopterin monophosphate and pyrophosphate. Required for efficient folate biosynthesis. Can also hydrolyze nucleoside triphosphates with a preference for dATP.~~[UniProtKB:P0AFC0]~~	+	[https://www.uniprot.org/uniprot/NUDB_ECOLI NUDB_ECOLI] Catalyzes the hydrolysis of dihydroneopterin triphosphate to dihydroneopterin monophosphate and pyrophosphate. Required for efficient folate biosynthesis. Can also hydrolyze nucleoside triphosphates with a preference for dATP.<ref>PMID:8798731</ref> <ref>PMID:17698004</ref>
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	Dihydroneopterin triphosphate pyrophosphatase (DHNTPase), a member of the Mg2+ dependent Nudix hydrolase superfamily, is the recently-discovered enzyme that functions in the second step of the pterin branch of the folate biosynthetic pathway in E. coli. DHNTPase is of interest because inhibition of enzymes in bacterial folate biosynthetic pathways is a strategy for antibiotic development. We determined crystal structures of DHNTPase with and without activating, Mg2+-mimicking metals Co2+ and Ni2+. Four metal ions, identified by anomalous scattering, and stoichiometrically confirmed in solution by isothermal titration calorimetry, are held in place by Glu56 and Glu60 within the Nudix sequence motif, Glu117, waters, and a sulfate ion, of which the latter is further stabilized by a salt bridge with Lys7. In silico docking of the DHNTP substrate reveals a binding mode in which the pterin ring moiety is nestled in a largely hydrophobic pocket, the beta-phosphate activated for nucleophilic attack overlays with the crystallographic sulfate and is in line with an activated water molecule, and remaining phosphate groups are stabilized by all four identified metal ions. The structures and binding data provide new details regarding DHNTPase metal requirements, mechanism, and suggest a strategy for efficient inhibition.	+
-		+
-	Metal ion coordination in the E. coli Nudix hydrolase dihydroneopterin triphosphate pyrophosphatase: New clues into catalytic mechanism.,Hill SE, Nguyen E, Ukachukwu CU, Freeman DM, Quirk S, Lieberman RL PLoS One. 2017 Jul 25;12(7):e0180241. doi: 10.1371/journal.pone.0180241., eCollection 2017. PMID:28742822<ref>PMID:~~28742822~~</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.~~<br>	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 5u7f" style="background-color~~:~~#fffaf0;">~~</~~div~~>	+
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Dihydroneopterin triphosphate diphosphatase]]~~	+	[[Category: Escherichia coli O157:H7]]
-	~~[[Category~~: ~~Eco57~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Hill~~, S E~~]]	+	[[Category: Hill SE]]
-	[[Category: Lieberman~~, R L~~]]	+	[[Category: Lieberman RL]]
-	[[Category: Nguyen, E]]	+	[[Category: Nguyen E]]
-	~~[[Category: Dihydroneopterin triphosphate pyrophosphohydrolase]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Metal ion binding]]~~	+
-	~~[[Category: Nudix hydrolase]]~~	+
-	~~[[Category: Tetranuclear~~]]	+

OCA at 07:06, 16 March 2022

OCA — Wed, 16 Mar 2022 07:06:43 GMT

←Older revision		Revision as of 07:06, 16 March 2022
Line 1:		Line 1:

	==Co-bound dihydroneopterin triphosphate pyrophosphohydrolase from E. coli==		==Co-bound dihydroneopterin triphosphate pyrophosphohydrolase from E. coli==
-	<StructureSection load='5u7f' size='340' side='right' caption='[[5u7f]], [[Resolution\|resolution]] 1.79Å' scene=''>	+	<StructureSection load='5u7f' size='340' side='right'caption='[[5u7f]], [[Resolution\|resolution]] 1.79Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5u7f]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Eco57 Eco57]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5U7F OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5U7F FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5u7f]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Eco57 Eco57]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5U7F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5U7F FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5u7e\|5u7e]], [[5u7h\|5u7h]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[5u7e\|5u7e]], [[5u7h\|5u7h]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">nudB, Z2917, ECs2575 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83334 ECO57])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">nudB, Z2917, ECs2575 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83334 ECO57])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Dihydroneopterin_triphosphate_diphosphatase Dihydroneopterin triphosphate diphosphatase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.67 3.6.1.67] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Dihydroneopterin_triphosphate_diphosphatase Dihydroneopterin triphosphate diphosphatase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.67 3.6.1.67] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5u7f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5u7f OCA], [~~http~~://pdbe.org/5u7f PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5u7f RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5u7f PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5u7f ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5u7f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5u7f OCA], [https://pdbe.org/5u7f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5u7f RCSB], [https://www.ebi.ac.uk/pdbsum/5u7f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5u7f ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/NUDB_ECO57 NUDB_ECO57]] Catalyzes the hydrolysis of dihydroneopterin triphosphate to dihydroneopterin monophosphate and pyrophosphate. Required for efficient folate biosynthesis. Can also hydrolyze nucleoside triphosphates with a preference for dATP.[UniProtKB:P0AFC0]	+	[[https://www.uniprot.org/uniprot/NUDB_ECO57 NUDB_ECO57]] Catalyzes the hydrolysis of dihydroneopterin triphosphate to dihydroneopterin monophosphate and pyrophosphate. Required for efficient folate biosynthesis. Can also hydrolyze nucleoside triphosphates with a preference for dATP.[UniProtKB:P0AFC0]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 27:		Line 27:
	[[Category: Dihydroneopterin triphosphate diphosphatase]]		[[Category: Dihydroneopterin triphosphate diphosphatase]]
	[[Category: Eco57]]		[[Category: Eco57]]
		+	[[Category: Large Structures]]
	[[Category: Hill, S E]]		[[Category: Hill, S E]]
	[[Category: Lieberman, R L]]		[[Category: Lieberman, R L]]

OCA at 10:21, 22 November 2017

OCA — Wed, 22 Nov 2017 10:21:29 GMT

←Older revision		Revision as of 10:21, 22 November 2017
Line 3:		Line 3:
	<StructureSection load='5u7f' size='340' side='right' caption='[[5u7f]], [[Resolution\|resolution]] 1.79Å' scene=''>		<StructureSection load='5u7f' size='340' side='right' caption='[[5u7f]], [[Resolution\|resolution]] 1.79Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5u7f]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5U7F OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5U7F FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5u7f]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Eco57 Eco57]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5U7F OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5U7F FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5u7e\|5u7e]], [[5u7h\|5u7h]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5u7e\|5u7e]], [[5u7h\|5u7h]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">nudB, Z2917, ECs2575 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83334 ECO57])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Dihydroneopterin_triphosphate_diphosphatase Dihydroneopterin triphosphate diphosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.67 3.6.1.67] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Dihydroneopterin_triphosphate_diphosphatase Dihydroneopterin triphosphate diphosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.67 3.6.1.67] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5u7f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5u7f OCA], [http://pdbe.org/5u7f PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5u7f RCSB], [http://www.ebi.ac.uk/pdbsum/5u7f PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5u7f ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5u7f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5u7f OCA], [http://pdbe.org/5u7f PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5u7f RCSB], [http://www.ebi.ac.uk/pdbsum/5u7f PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5u7f ProSAT]</span></td></tr>
Line 25:		Line 26:
	</StructureSection>		</StructureSection>
	[[Category: Dihydroneopterin triphosphate diphosphatase]]		[[Category: Dihydroneopterin triphosphate diphosphatase]]
		+	[[Category: Eco57]]
	[[Category: Hill, S E]]		[[Category: Hill, S E]]
	[[Category: Lieberman, R L]]		[[Category: Lieberman, R L]]

OCA at 09:14, 9 August 2017

OCA — Wed, 09 Aug 2017 09:14:39 GMT

←Older revision		Revision as of 09:14, 9 August 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5u7f is ~~ON HOLD until Paper~~ Publication	+	==Co-bound dihydroneopterin triphosphate pyrophosphohydrolase from E. coli==
		+	<StructureSection load='5u7f' size='340' side='right' caption='[[5u7f]], [[Resolution\|resolution]] 1.79Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5u7f]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5U7F OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5U7F FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5u7e\|5u7e]], [[5u7h\|5u7h]]</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Dihydroneopterin_triphosphate_diphosphatase Dihydroneopterin triphosphate diphosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.67 3.6.1.67] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5u7f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5u7f OCA], [http://pdbe.org/5u7f PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5u7f RCSB], [http://www.ebi.ac.uk/pdbsum/5u7f PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5u7f ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/NUDB_ECO57 NUDB_ECO57]] Catalyzes the hydrolysis of dihydroneopterin triphosphate to dihydroneopterin monophosphate and pyrophosphate. Required for efficient folate biosynthesis. Can also hydrolyze nucleoside triphosphates with a preference for dATP.[UniProtKB:P0AFC0]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Dihydroneopterin triphosphate pyrophosphatase (DHNTPase), a member of the Mg2+ dependent Nudix hydrolase superfamily, is the recently-discovered enzyme that functions in the second step of the pterin branch of the folate biosynthetic pathway in E. coli. DHNTPase is of interest because inhibition of enzymes in bacterial folate biosynthetic pathways is a strategy for antibiotic development. We determined crystal structures of DHNTPase with and without activating, Mg2+-mimicking metals Co2+ and Ni2+. Four metal ions, identified by anomalous scattering, and stoichiometrically confirmed in solution by isothermal titration calorimetry, are held in place by Glu56 and Glu60 within the Nudix sequence motif, Glu117, waters, and a sulfate ion, of which the latter is further stabilized by a salt bridge with Lys7. In silico docking of the DHNTP substrate reveals a binding mode in which the pterin ring moiety is nestled in a largely hydrophobic pocket, the beta-phosphate activated for nucleophilic attack overlays with the crystallographic sulfate and is in line with an activated water molecule, and remaining phosphate groups are stabilized by all four identified metal ions. The structures and binding data provide new details regarding DHNTPase metal requirements, mechanism, and suggest a strategy for efficient inhibition.

-	~~Authors: Nguyen,~~ E., Hill, S.E., ~~Lieberman~~, R.L.	+	Metal ion coordination in the E. coli Nudix hydrolase dihydroneopterin triphosphate pyrophosphatase: New clues into catalytic mechanism.,Hill SE, Nguyen E, Ukachukwu CU, Freeman DM, Quirk S, Lieberman RL PLoS One. 2017 Jul 25;12(7):e0180241. doi: 10.1371/journal.pone.0180241., eCollection 2017. PMID:28742822<ref>PMID:28742822</ref>

-	~~Description: Co~~-~~bound dihydroneopterin~~ triphosphate ~~pyrophosphohydrolase from E. coli~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
-	[[Category: Lieberman, R.L]]	+	<div class="pdbe-citations 5u7f" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Dihydroneopterin triphosphate diphosphatase]]
		+	[[Category: Hill, S E]]
		+	[[Category: Lieberman, R L]]
	[[Category: Nguyen, E]]		[[Category: Nguyen, E]]
-	[[Category: ~~Hill, S.E~~]]	+	[[Category: Dihydroneopterin triphosphate pyrophosphohydrolase]]
		+	[[Category: Hydrolase]]
		+	[[Category: Metal ion binding]]
		+	[[Category: Nudix hydrolase]]
		+	[[Category: Tetranuclear]]

OCA at 18:48, 15 March 2017

OCA — Wed, 15 Mar 2017 18:48:57 GMT

←Older revision		Revision as of 18:48, 15 March 2017
Line 7:		Line 7:
	Description: Co-bound dihydroneopterin triphosphate pyrophosphohydrolase from E. coli		Description: Co-bound dihydroneopterin triphosphate pyrophosphohydrolase from E. coli
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Nguyen, E]]
	[[Category: Lieberman, R.L]]		[[Category: Lieberman, R.L]]
		+	[[Category: Nguyen, E]]
	[[Category: Hill, S.E]]		[[Category: Hill, S.E]]

OCA at 14:14, 9 March 2017

OCA — Thu, 09 Mar 2017 14:14:06 GMT

←Older revision		Revision as of 14:14, 9 March 2017
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5u7f is ON HOLD	+	The entry 5u7f is ON HOLD until Paper Publication

	Authors: Nguyen, E., Hill, S.E., Lieberman, R.L.		Authors: Nguyen, E., Hill, S.E., Lieberman, R.L.

OCA at 15:09, 2 January 2017

OCA — Mon, 02 Jan 2017 15:09:08 GMT

←Older revision		Revision as of 15:09, 2 January 2017
Line 3:		Line 3:
	The entry 5u7f is ON HOLD		The entry 5u7f is ON HOLD

-	Authors: Nguyen, ~~Elaine~~, Hill, ~~Shannon~~ E., Lieberman, ~~Raquel~~ L.	+	Authors: Nguyen, E., Hill, S.E., Lieberman, R.L.

	Description: Co-bound dihydroneopterin triphosphate pyrophosphohydrolase from E. coli		Description: Co-bound dihydroneopterin triphosphate pyrophosphohydrolase from E. coli
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Nguyen, ~~Elaine, Hill, Shannon~~ E]]	+	[[Category: Nguyen, E]]
-	[[Category: Lieberman, ~~Raquel~~ L]]	+	[[Category: Lieberman, R.L]]
		+	[[Category: Hill, S.E]]

OCA: Protected "5u7f" [edit=sysop:move=sysop]

OCA — Thu, 22 Dec 2016 20:29:48 GMT

Protected "5u7f" [edit=sysop:move=sysop]

←Older revision

Revision as of 20:29, 22 December 2016

OCA: New page: '''Unreleased structure''' The entry 5u7f is ON HOLD Authors: Nguyen, Elaine, Hill, Shannon E., Lieberman, Raquel L. Description: Co-bound dihydroneopterin triphosphate pyrophosphohydr...

OCA — Thu, 22 Dec 2016 20:29:46 GMT

New page: '''Unreleased structure''' The entry 5u7f is ON HOLD Authors: Nguyen, Elaine, Hill, Shannon E., Lieberman, Raquel L. Description: Co-bound dihydroneopterin triphosphate pyrophosphohydr...

New page

'''Unreleased structure'''

The entry 5u7f is ON HOLD

Authors: Nguyen, Elaine, Hill, Shannon E., Lieberman, Raquel L.

Description: Co-bound dihydroneopterin triphosphate pyrophosphohydrolase from E. coli
[[Category: Unreleased Structures]]
[[Category: Nguyen, Elaine, Hill, Shannon E]]
[[Category: Lieberman, Raquel L]]