5udz - Revision history

OCA at 13:26, 4 October 2023

OCA — Wed, 04 Oct 2023 13:26:07 GMT

←Older revision		Revision as of 13:26, 4 October 2023
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	<StructureSection load='5udz' size='340' side='right'caption='[[5udz]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='5udz' size='340' side='right'caption='[[5udz]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5udz]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UDZ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5UDZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5udz]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UDZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5UDZ FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat"~~><scene name~~=~~'pdbligand=ZN:ZINC+ION'>ZN</scene~~></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~LIN28A, CSDD1, LIN28, ZCCHC1 ([http~~:/~~/www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5udz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5udz OCA], [~~http~~://pdbe.org/5udz PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5udz RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5udz PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5udz ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5udz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5udz OCA], [https://pdbe.org/5udz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5udz RCSB], [https://www.ebi.ac.uk/pdbsum/5udz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5udz ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/LN28A_HUMAN LN28A_HUMAN]] Acts as a 'translational enhancer', driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis. Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in stabilizing the mRNAs. Binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA. Essential for skeletal muscle differentiation program through the translational up-regulation of IGF2 expression (By similarity). Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre-let-7 in embryonic stem (ES) cells contributes to the maintenance of ES cells. In contrast, LIN28A down-regulation in neural stem cells by miR-125, allows the processing of pre-let-7. Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation.<ref>PMID:18951094</ref> <ref>PMID:19703396</ref>	+	[https://www.uniprot.org/uniprot/LN28A_HUMAN LN28A_HUMAN] Acts as a 'translational enhancer', driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis. Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in stabilizing the mRNAs. Binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA. Essential for skeletal muscle differentiation program through the translational up-regulation of IGF2 expression (By similarity). Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre-let-7 in embryonic stem (ES) cells contributes to the maintenance of ES cells. In contrast, LIN28A down-regulation in neural stem cells by miR-125, allows the processing of pre-let-7. Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation.<ref>PMID:18951094</ref> <ref>PMID:19703396</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Nam, Y]]	+	[[Category: Nam Y]]
-	[[Category: Sliz, P]]	+	[[Category: Sliz P]]
-	[[Category: Wang, L]]	+	[[Category: Wang L]]
-	~~[[Category: Let-7]]~~	+
-	~~[[Category: Lin28]]~~	+
-	~~[[Category: Microrna]]~~	+
-	~~[[Category: Rna binding protein-rna complex~~]]	+

OCA at 09:28, 4 December 2019

OCA — Wed, 04 Dec 2019 09:28:50 GMT

←Older revision		Revision as of 09:28, 4 December 2019
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	==Human LIN28A in complex with let-7f-1 microRNA pre-element==		==Human LIN28A in complex with let-7f-1 microRNA pre-element==
-	<StructureSection load='5udz' size='340' side='right' caption='[[5udz]], [[Resolution\|resolution]] 2.00Å' scene=''>	+	<StructureSection load='5udz' size='340' side='right'caption='[[5udz]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5udz]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UDZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5UDZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5udz]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UDZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5UDZ FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">LIN28A, CSDD1, LIN28, ZCCHC1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5udz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5udz OCA], [http://pdbe.org/5udz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5udz RCSB], [http://www.ebi.ac.uk/pdbsum/5udz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5udz ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5udz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5udz OCA], [http://pdbe.org/5udz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5udz RCSB], [http://www.ebi.ac.uk/pdbsum/5udz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5udz ProSAT]</span></td></tr>
	</table>		</table>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Nam, Y]]		[[Category: Nam, Y]]
	[[Category: Sliz, P]]		[[Category: Sliz, P]]

OCA at 07:04, 29 March 2017

OCA — Wed, 29 Mar 2017 07:04:55 GMT

←Older revision		Revision as of 07:04, 29 March 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5udz is ~~ON HOLD~~ ~~until Paper~~ Publication	+	==Human LIN28A in complex with let-7f-1 microRNA pre-element==
		+	<StructureSection load='5udz' size='340' side='right' caption='[[5udz]], [[Resolution\|resolution]] 2.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5udz]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UDZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5UDZ FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5udz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5udz OCA], [http://pdbe.org/5udz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5udz RCSB], [http://www.ebi.ac.uk/pdbsum/5udz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5udz ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/LN28A_HUMAN LN28A_HUMAN]] Acts as a 'translational enhancer', driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis. Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in stabilizing the mRNAs. Binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA. Essential for skeletal muscle differentiation program through the translational up-regulation of IGF2 expression (By similarity). Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre-let-7 in embryonic stem (ES) cells contributes to the maintenance of ES cells. In contrast, LIN28A down-regulation in neural stem cells by miR-125, allows the processing of pre-let-7. Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation.<ref>PMID:18951094</ref> <ref>PMID:19703396</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	LIN28 is an RNA binding protein that plays crucial roles in pluripotency, glucose metabolism, tissue regeneration, and tumorigenesis. LIN28 binds to the let-7 primary and precursor microRNAs through bipartite recognition and induces degradation of let-7 precursors (pre-let-7) by promoting oligouridylation by terminal uridylyltransferases (TUTases). Here, we report that the zinc knuckle domain (ZKD) of mouse LIN28 recruits TUT4 to initiate the oligouridylation of let-7 precursors. Our crystal structure of human LIN28 in complex with a fragment of pre-let-7f-1 determined to 2.0 A resolution shows that the interaction between ZKD and RNA is constrained to a small cavity with a high druggability score. We demonstrate that the specific interaction between ZKD and pre-let-7 is necessary and sufficient to induce oligouridylation by recruiting the N-terminal fragment of TUT4 (NTUT4) and the formation of a stable ZKD:NTUT4:pre-let-7 ternary complex is crucial for the acquired processivity of TUT4.

-	~~Authors~~:	+	LIN28 Zinc Knuckle Domain Is Required and Sufficient to Induce let-7 Oligouridylation.,Wang L, Nam Y, Lee AK, Yu C, Roth K, Chen C, Ransey EM, Sliz P Cell Rep. 2017 Mar 14;18(11):2664-2675. doi: 10.1016/j.celrep.2017.02.044. PMID:28297670<ref>PMID:28297670</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 5udz" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Nam, Y]]
		+	[[Category: Sliz, P]]
		+	[[Category: Wang, L]]
		+	[[Category: Let-7]]
		+	[[Category: Lin28]]
		+	[[Category: Microrna]]
		+	[[Category: Rna binding protein-rna complex]]

OCA at 14:23, 9 March 2017

OCA — Thu, 09 Mar 2017 14:23:17 GMT

←Older revision		Revision as of 14:23, 9 March 2017
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5udz is ON HOLD	+	The entry 5udz is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "5udz" [edit=sysop:move=sysop]

OCA — Wed, 04 Jan 2017 15:43:34 GMT

Protected "5udz" [edit=sysop:move=sysop]

←Older revision

Revision as of 15:43, 4 January 2017

OCA: New page: '''Unreleased structure''' The entry 5udz is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 04 Jan 2017 15:43:33 GMT

New page: '''Unreleased structure''' The entry 5udz is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 5udz is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]