5ufy - Revision history

OCA at 14:27, 6 March 2024

OCA — Wed, 06 Mar 2024 14:27:03 GMT

←Older revision		Revision as of 14:27, 6 March 2024
Line 1:		Line 1:

	==Structure of Streptococcus pneumoniae peptidoglycan O-acetyltransferase A (OatA) C-terminal catalytic domain==		==Structure of Streptococcus pneumoniae peptidoglycan O-acetyltransferase A (OatA) C-terminal catalytic domain==
-	<StructureSection load='5ufy' size='340' side='right' caption='[[5ufy]], [[Resolution\|resolution]] 1.12Å' scene=''>	+	<StructureSection load='5ufy' size='340' side='right'caption='[[5ufy]], [[Resolution\|resolution]] 1.12Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5ufy]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"diplococcus_pneumoniae"_(klein_1884)_weichselbaum_1886 "diplococcus~~ pneumoniae~~" (klein 1884) weichselbaum 1886~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UFY OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5UFY FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5ufy]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_pneumoniae Streptococcus pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UFY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5UFY FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat"~~><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.12Å</td></tr>
-	~~<tr~~ id~~='related'><td class~~="~~sblockLbl~~">~~<b>~~[[~~Related_structure~~\|~~Related:]]</b></td><td class="sblockDat">[[5ug1\|5ug1~~]]</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">oatA_2, oatA, oatA_1, oatA_3, ERS020138_01729, ERS020158_01919, ERS020525_00845, ERS020528_00979, ERS020532_00615, ERS020726_01157, ERS020726_01160, ERS020726_01165, ERS021733_04500, ERS232524_00457 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=~~1313~~ "~~Diplococcus pneumoniae~~" ~~(Klein 1884) Weichselbaum 1886])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ufy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ufy OCA], [https://pdbe.org/5ufy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ufy RCSB], [https://www.ebi.ac.uk/pdbsum/5ufy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ufy ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5ufy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ufy OCA], [~~http~~://pdbe.org/5ufy PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5ufy RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5ufy PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5ufy ProSAT]</span></td></tr>	+
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/Q8CY83_STRR6 Q8CY83_STRR6]
-	The O-acetylation of the essential cell wall polymer peptidoglycan occurs in most Gram-positive bacterial pathogens, including species of Staphylococcus, Streptococcus and Enterococcus. This modification to peptidoglycan protects these pathogens from the lytic action of the lysozymes of innate immunity systems and, as such, is recognized as a virulence factor. The key enzyme involved, peptidoglycan O-acetyltransferase A (OatA) represents a particular challenge to biochemical study since it is a membrane associated protein whose substrate is the insoluble peptidoglycan cell wall polymer. OatA is predicted to be bimodular, being comprised of an N-terminal integral membrane domain linked to a C-terminal extracytoplasmic domain. We present herein the first biochemical and kinetic characterization of the C-terminal catalytic domain of OatA from two important human pathogens, Staphylococcus aureus and Streptococcus pneumoniae. Using both pseudosubstrates and novel biosynthetically-prepared peptidoglycan polymers, we characterized distinct substrate specificities for the two enzymes. In addition, the high resolution crystal structure of the C-terminal domain reveals an SGNH/GDSL-like hydrolase fold with a catalytic triad of amino acids but with a non-canonical oxyanion hole structure. Site-specific replacements confirmed the identity of the catalytic and oxyanion hole residues. A model is presented for the O-acetylation of peptidoglycan whereby the translocation of acetyl groups from a cytoplasmic source across the cytoplasmic membrane is catalyzed by the N-terminal domain of OatA for their transfer to peptidoglycan by its C-terminal domain. This study on the structure-function relationship of OatA provides a molecular and mechanistic understanding of this bacterial resistance mechanism opening the prospect for novel chemotherapeutic exploration to enhance innate immunity protection against Gram-positive pathogens.	+
-		+
-	In vitro characterization of the antivirulence target of Gram-positive pathogens, peptidoglycan O-acetyltransferase A (OatA).,Sychantha D, Jones CS, Little DJ, Moynihan PJ, Robinson H, Galley NF, Roper DI, Dowson CG, Howell PL, Clarke AJ PLoS Pathog. 2017 Oct 27;13(10):~~e1006667. doi: 10.1371~~/~~journal.ppat.1006667., eCollection 2017 Oct. PMID:29077761<ref>PMID:29077761<~~/~~ref>~~	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U~~.S. ~~National Library of Medicine.<br>~~	+
-	</~~div>~~	+
-	~~<div class="pdbe-citations 5ufy" style="background-color:#fffaf0;"></div>~~	+
-	~~== References ==~~	+
-	~~<references~~/>	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Clarke, A J~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Dowson, C G~~]]	+	[[Category: Streptococcus pneumoniae]]
-	[[Category: ~~Galley, N F~~]]	+	[[Category: Clarke AJ]]
-	[[Category: ~~Howell, P L~~]]	+	[[Category: Dowson CG]]
-	[[Category: ~~Jones, C~~]]	+	[[Category: Galley NF]]
-	[[Category: ~~Little, D J~~]]	+	[[Category: Howell PL]]
-	[[Category: ~~Moynihan, P J~~]]	+	[[Category: Jones C]]
-	[[Category: ~~Robinson, H~~]]	+	[[Category: Little DJ]]
-	[[Category: ~~Roper, D I~~]]	+	[[Category: Moynihan PJ]]
-	[[Category: ~~Sychantha, D~~]]	+	[[Category: Robinson H]]
-	[[Category: ~~Atytpical alpha/beta hydrolase~~]]	+	[[Category: Roper DI]]
-	[[Category: ~~Catalytic triad]]~~	+	[[Category: Sychantha D]]
-	~~[[Category: Transferase~~]]	+

OCA at 07:47, 6 December 2017

OCA — Wed, 06 Dec 2017 07:47:17 GMT

←Older revision		Revision as of 07:47, 6 December 2017
Line 3:		Line 3:
	<StructureSection load='5ufy' size='340' side='right' caption='[[5ufy]], [[Resolution\|resolution]] 1.12Å' scene=''>		<StructureSection load='5ufy' size='340' side='right' caption='[[5ufy]], [[Resolution\|resolution]] 1.12Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5ufy]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UFY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5UFY FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5ufy]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"diplococcus_pneumoniae"_(klein_1884)_weichselbaum_1886 "diplococcus pneumoniae" (klein 1884) weichselbaum 1886]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UFY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5UFY FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5ug1\|5ug1]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5ug1\|5ug1]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">oatA_2, oatA, oatA_1, oatA_3, ERS020138_01729, ERS020158_01919, ERS020525_00845, ERS020528_00979, ERS020532_00615, ERS020726_01157, ERS020726_01160, ERS020726_01165, ERS021733_04500, ERS232524_00457 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1313 "Diplococcus pneumoniae" (Klein 1884) Weichselbaum 1886])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ufy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ufy OCA], [http://pdbe.org/5ufy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ufy RCSB], [http://www.ebi.ac.uk/pdbsum/5ufy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ufy ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ufy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ufy OCA], [http://pdbe.org/5ufy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ufy RCSB], [http://www.ebi.ac.uk/pdbsum/5ufy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ufy ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The O-acetylation of the essential cell wall polymer peptidoglycan occurs in most Gram-positive bacterial pathogens, including species of Staphylococcus, Streptococcus and Enterococcus. This modification to peptidoglycan protects these pathogens from the lytic action of the lysozymes of innate immunity systems and, as such, is recognized as a virulence factor. The key enzyme involved, peptidoglycan O-acetyltransferase A (OatA) represents a particular challenge to biochemical study since it is a membrane associated protein whose substrate is the insoluble peptidoglycan cell wall polymer. OatA is predicted to be bimodular, being comprised of an N-terminal integral membrane domain linked to a C-terminal extracytoplasmic domain. We present herein the first biochemical and kinetic characterization of the C-terminal catalytic domain of OatA from two important human pathogens, Staphylococcus aureus and Streptococcus pneumoniae. Using both pseudosubstrates and novel biosynthetically-prepared peptidoglycan polymers, we characterized distinct substrate specificities for the two enzymes. In addition, the high resolution crystal structure of the C-terminal domain reveals an SGNH/GDSL-like hydrolase fold with a catalytic triad of amino acids but with a non-canonical oxyanion hole structure. Site-specific replacements confirmed the identity of the catalytic and oxyanion hole residues. A model is presented for the O-acetylation of peptidoglycan whereby the translocation of acetyl groups from a cytoplasmic source across the cytoplasmic membrane is catalyzed by the N-terminal domain of OatA for their transfer to peptidoglycan by its C-terminal domain. This study on the structure-function relationship of OatA provides a molecular and mechanistic understanding of this bacterial resistance mechanism opening the prospect for novel chemotherapeutic exploration to enhance innate immunity protection against Gram-positive pathogens.
		+
		+	In vitro characterization of the antivirulence target of Gram-positive pathogens, peptidoglycan O-acetyltransferase A (OatA).,Sychantha D, Jones CS, Little DJ, Moynihan PJ, Robinson H, Galley NF, Roper DI, Dowson CG, Howell PL, Clarke AJ PLoS Pathog. 2017 Oct 27;13(10):e1006667. doi: 10.1371/journal.ppat.1006667., eCollection 2017 Oct. PMID:29077761<ref>PMID:29077761</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5ufy" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 07:16, 25 October 2017

OCA — Wed, 25 Oct 2017 07:16:50 GMT

←Older revision		Revision as of 07:16, 25 October 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5ufy is ~~ON HOLD until Paper Publication~~	+	==Structure of Streptococcus pneumoniae peptidoglycan O-acetyltransferase A (OatA) C-terminal catalytic domain==
-		+	<StructureSection load='5ufy' size='340' side='right' caption='[[5ufy]], [[Resolution\|resolution]] 1.12Å' scene=''>
-	~~Authors~~: ~~Sychantha, D~~.~~, Jones, C~~.~~, Little, D~~.J., ~~Moynihan, P~~.J., ~~Robinson~~, H., ~~Galley, N~~.F., ~~Roper, D~~.I., Dowson, C.G., Howell, P.L~~., Clarke, A.J.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5ufy]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UFY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5UFY FirstGlance]. <br>
-	~~Description~~: ~~Structure of Streptococcus pneumoniae peptidoglycan O-acetyltransferase A (OatA)~~ C~~-terminal catalytic domain~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5ug1\|5ug1]]</td></tr>
-	[[Category: ~~Clarke~~, A.J]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ufy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ufy OCA], [http://pdbe.org/5ufy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ufy RCSB], [http://www.ebi.ac.uk/pdbsum/5ufy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ufy ProSAT]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Clarke, A J]]
		+	[[Category: Dowson, C G]]
		+	[[Category: Galley, N F]]
		+	[[Category: Howell, P L]]
		+	[[Category: Jones, C]]
		+	[[Category: Little, D J]]
		+	[[Category: Moynihan, P J]]
	[[Category: Robinson, H]]		[[Category: Robinson, H]]
		+	[[Category: Roper, D I]]
	[[Category: Sychantha, D]]		[[Category: Sychantha, D]]
-	[[Category: ~~Roper, D.I~~]]	+	[[Category: Atytpical alpha/beta hydrolase]]
-	[[Category: ~~Jones, C~~]]	+	[[Category: Catalytic triad]]
-	[[Category: ~~Little, D.J]]~~	+	[[Category: Transferase]]
-	~~[[Category: Howell, P.L]]~~	+
-	~~[[Category: Moynihan, P.J]]~~	+
-	~~[[Category: Dowson, C.G]]~~	+
-	~~[[Category: Galley, N.F~~]]	+

OCA at 13:38, 27 September 2017

OCA — Wed, 27 Sep 2017 13:38:10 GMT

←Older revision		Revision as of 13:38, 27 September 2017
Line 7:		Line 7:
	Description: Structure of Streptococcus pneumoniae peptidoglycan O-acetyltransferase A (OatA) C-terminal catalytic domain		Description: Structure of Streptococcus pneumoniae peptidoglycan O-acetyltransferase A (OatA) C-terminal catalytic domain
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Howell, P.L]]
-	[[Category: Moynihan, P.J]]
-	[[Category: Dowson, C.G]]
	[[Category: Clarke, A.J]]		[[Category: Clarke, A.J]]
	[[Category: Robinson, H]]		[[Category: Robinson, H]]
	[[Category: Sychantha, D]]		[[Category: Sychantha, D]]
	[[Category: Roper, D.I]]		[[Category: Roper, D.I]]
-	[[Category: Galley, N.F]]
	[[Category: Jones, C]]		[[Category: Jones, C]]
	[[Category: Little, D.J]]		[[Category: Little, D.J]]
		+	[[Category: Howell, P.L]]
		+	[[Category: Moynihan, P.J]]
		+	[[Category: Dowson, C.G]]
		+	[[Category: Galley, N.F]]

OCA at 19:01, 15 March 2017

OCA — Wed, 15 Mar 2017 19:01:11 GMT

←Older revision		Revision as of 19:01, 15 March 2017
Line 7:		Line 7:
	Description: Structure of Streptococcus pneumoniae peptidoglycan O-acetyltransferase A (OatA) C-terminal catalytic domain		Description: Structure of Streptococcus pneumoniae peptidoglycan O-acetyltransferase A (OatA) C-terminal catalytic domain
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
		+	[[Category: Howell, P.L]]
		+	[[Category: Moynihan, P.J]]
		+	[[Category: Dowson, C.G]]
	[[Category: Clarke, A.J]]		[[Category: Clarke, A.J]]
	[[Category: Robinson, H]]		[[Category: Robinson, H]]
	[[Category: Sychantha, D]]		[[Category: Sychantha, D]]
	[[Category: Roper, D.I]]		[[Category: Roper, D.I]]
		+	[[Category: Galley, N.F]]
	[[Category: Jones, C]]		[[Category: Jones, C]]
	[[Category: Little, D.J]]		[[Category: Little, D.J]]
-	[[Category: Howell, P.L]]
-	[[Category: Moynihan, P.J]]
-	[[Category: Dowson, C.G]]
-	[[Category: Galley, N.F]]

OCA at 14:26, 9 March 2017

OCA — Thu, 09 Mar 2017 14:26:05 GMT

←Older revision		Revision as of 14:26, 9 March 2017
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5ufy is ON HOLD	+	The entry 5ufy is ON HOLD until Paper Publication

	Authors: Sychantha, D., Jones, C., Little, D.J., Moynihan, P.J., Robinson, H., Galley, N.F., Roper, D.I., Dowson, C.G., Howell, P.L., Clarke, A.J.		Authors: Sychantha, D., Jones, C., Little, D.J., Moynihan, P.J., Robinson, H., Galley, N.F., Roper, D.I., Dowson, C.G., Howell, P.L., Clarke, A.J.

-	Description: Structure of Streptococcus pneumoniae peptidoglycan O-acetyltransferase A (OatA)	+	Description: Structure of Streptococcus pneumoniae peptidoglycan O-acetyltransferase A (OatA) C-terminal catalytic domain
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Clarke, A.J]]		[[Category: Clarke, A.J]]

OCA: Protected "5ufy" [edit=sysop:move=sysop]

OCA — Wed, 18 Jan 2017 14:55:07 GMT

Protected "5ufy" [edit=sysop:move=sysop]

←Older revision

Revision as of 14:55, 18 January 2017

OCA: New page: '''Unreleased structure''' The entry 5ufy is ON HOLD Authors: Sychantha, D., Jones, C., Little, D.J., Moynihan, P.J., Robinson, H., Galley, N.F., Roper, D.I., Dowson, C.G., Howell, P.L....

OCA — Wed, 18 Jan 2017 14:55:05 GMT

New page: '''Unreleased structure''' The entry 5ufy is ON HOLD Authors: Sychantha, D., Jones, C., Little, D.J., Moynihan, P.J., Robinson, H., Galley, N.F., Roper, D.I., Dowson, C.G., Howell, P.L....

New page

'''Unreleased structure'''

The entry 5ufy is ON HOLD

Authors: Sychantha, D., Jones, C., Little, D.J., Moynihan, P.J., Robinson, H., Galley, N.F., Roper, D.I., Dowson, C.G., Howell, P.L., Clarke, A.J.

Description: Structure of Streptococcus pneumoniae peptidoglycan O-acetyltransferase A (OatA)
[[Category: Unreleased Structures]]
[[Category: Clarke, A.J]]
[[Category: Robinson, H]]
[[Category: Sychantha, D]]
[[Category: Roper, D.I]]
[[Category: Jones, C]]
[[Category: Little, D.J]]
[[Category: Howell, P.L]]
[[Category: Moynihan, P.J]]
[[Category: Dowson, C.G]]
[[Category: Galley, N.F]]