5ugk - Revision history

OCA at 10:25, 14 June 2023

OCA — Wed, 14 Jun 2023 10:25:46 GMT

←Older revision		Revision as of 10:25, 14 June 2023
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	==Zinc-Binding Structure of a Catalytic Amyloid from Solid-State NMR Spectroscopy==		==Zinc-Binding Structure of a Catalytic Amyloid from Solid-State NMR Spectroscopy==
-	<StructureSection load='5ugk' size='340' side='right'caption='[[5ugk~~]], [[NMR_Ensembles_of_Models \| 20 NMR models~~]]' scene=''>	+	<StructureSection load='5ugk' size='340' side='right'caption='[[5ugk]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5ugk]] is a 12 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UGK OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5UGK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5ugk]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UGK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5UGK FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5ugk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ugk OCA], [~~http~~://pdbe.org/5ugk PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5ugk RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5ugk PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5ugk ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ugk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ugk OCA], [https://pdbe.org/5ugk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ugk RCSB], [https://www.ebi.ac.uk/pdbsum/5ugk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ugk ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: DeGrado~~, W F~~]]	+	[[Category: Synthetic construct]]
-	[[Category: Gosavi~~, P M~~]]	+	[[Category: DeGrado WF]]
-	[[Category: Hong, M]]	+	[[Category: Gosavi PM]]
-	[[Category: Korendovych~~, I V~~]]	+	[[Category: Hong M]]
-	[[Category: Lee, M]]	+	[[Category: Korendovych IV]]
-	[[Category: Makhlynets~~, O V~~]]	+	[[Category: Lee M]]
-	[[Category: Polizzi, N]]	+	[[Category: Makhlynets OV]]
-	[[Category: Wang, T]]	+	[[Category: Polizzi N]]
-	[[Category: Wu, H]]	+	[[Category: Wang T]]
-	[[Category: Wu, Y]]	+	[[Category: Wu H]]
-	~~[[Category: Amyloid]]~~	+	[[Category: Wu Y]]
-	~~[[Category: Metal binding protein~~]]	+

OCA at 09:06, 18 December 2019

OCA — Wed, 18 Dec 2019 09:06:27 GMT

←Older revision		Revision as of 09:06, 18 December 2019
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	==Zinc-Binding Structure of a Catalytic Amyloid from Solid-State NMR Spectroscopy==		==Zinc-Binding Structure of a Catalytic Amyloid from Solid-State NMR Spectroscopy==
-	<StructureSection load='5ugk' size='340' side='right' caption='[[5ugk]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>	+	<StructureSection load='5ugk' size='340' side='right'caption='[[5ugk]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5ugk]] is a 12 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UGK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5UGK FirstGlance]. <br>		<table><tr><td colspan='2'>[[5ugk]] is a 12 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UGK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5UGK FirstGlance]. <br>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: DeGrado, W F]]		[[Category: DeGrado, W F]]
	[[Category: Gosavi, P M]]		[[Category: Gosavi, P M]]

OCA at 09:16, 3 August 2017

OCA — Thu, 03 Aug 2017 09:16:03 GMT

←Older revision		Revision as of 09:16, 3 August 2017
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-	'''Unreleased structure'''

-	~~The entry~~ 5ugk is ~~ON HOLD until Paper~~ Publication	+	==Zinc-Binding Structure of a Catalytic Amyloid from Solid-State NMR Spectroscopy==
		+	<StructureSection load='5ugk' size='340' side='right' caption='[[5ugk]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5ugk]] is a 12 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UGK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5UGK FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ugk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ugk OCA], [http://pdbe.org/5ugk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ugk RCSB], [http://www.ebi.ac.uk/pdbsum/5ugk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ugk ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Throughout biology, amyloids are key structures in both functional proteins and the end product of pathologic protein misfolding. Amyloids might also represent an early precursor in the evolution of life because of their small molecular size and their ability to self-purify and catalyze chemical reactions. They also provide attractive backbones for advanced materials. When beta-strands of an amyloid are arranged parallel and in register, side chains from the same position of each chain align, facilitating metal chelation when the residues are good ligands such as histidine. High-resolution structures of metalloamyloids are needed to understand the molecular bases of metal-amyloid interactions. Here we combine solid-state NMR and structural bioinformatics to determine the structure of a zinc-bound metalloamyloid that catalyzes ester hydrolysis. The peptide forms amphiphilic parallel beta-sheets that assemble into stacked bilayers with alternating hydrophobic and polar interfaces. The hydrophobic interface is stabilized by apolar side chains from adjacent sheets, whereas the hydrated polar interface houses the Zn2+-binding histidines with binding geometries unusual in proteins. Each Zn2+ has two bis-coordinated histidine ligands, which bridge adjacent strands to form an infinite metal-ligand chain along the fibril axis. A third histidine completes the protein ligand environment, leaving a free site on the Zn2+ for water activation. This structure defines a class of materials, which we call metal-peptide frameworks. The structure reveals a delicate interplay through which metal ions stabilize the amyloid structure, which in turn shapes the ligand geometry and catalytic reactivity of Zn2.

-	~~Authors:~~ Lee, M., Wang, T., Makhlynets~~, O.V.~~, Wu, Y., Polizzi~~, N.~~, Wu, H., Gosavi, ~~P.M.~~, Korendovych~~, I.V.~~, DeGrado, W.F., ~~Hong, M~~.	+	Zinc-binding structure of a catalytic amyloid from solid-state NMR.,Lee M, Wang T, Makhlynets OV, Wu Y, Polizzi NF, Wu H, Gosavi PM, Stohr J, Korendovych IV, DeGrado WF, Hong M Proc Natl Acad Sci U S A. 2017 Jun 13;114(24):6191-6196. doi:, 10.1073/pnas.1706179114. Epub 2017 May 31. PMID:28566494<ref>PMID:28566494</ref>

-	~~Description: Zinc-Binding Structure~~ of ~~a Catalytic Amyloid from Solid-State NMR Spectroscopy~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category: Unreleased Structures]]~~	+	</div>
-	~~[[Category~~: ~~Degrado, W.F]]~~	+	<div class="pdbe-citations 5ugk" style="background-color:#fffaf0;"></div>
-	~~[[Category: Korendovych, I.V]]~~	+	== References ==
-	~~[[Category: Wang, T]]~~	+	<references/>
-	~~[[Category: Makhlynets, O.V]]~~	+	__TOC__
-	~~[[Category: Polizzi, N]]~~	+	</StructureSection>
-	[[Category: Wu, Y]]	+	[[Category: DeGrado, W F]]
-	[[Category: Gosavi, P.M]]	+	[[Category: Gosavi, P M]]
	[[Category: Hong, M]]		[[Category: Hong, M]]
-	[[Category: Wu, H]]	+	[[Category: Korendovych, I V]]
	[[Category: Lee, M]]		[[Category: Lee, M]]
		+	[[Category: Makhlynets, O V]]
		+	[[Category: Polizzi, N]]
		+	[[Category: Wang, T]]
		+	[[Category: Wu, H]]
		+	[[Category: Wu, Y]]
		+	[[Category: Amyloid]]
		+	[[Category: Metal binding protein]]

OCA at 19:02, 15 March 2017

OCA — Wed, 15 Mar 2017 19:02:25 GMT

←Older revision		Revision as of 19:02, 15 March 2017
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	Description: Zinc-Binding Structure of a Catalytic Amyloid from Solid-State NMR Spectroscopy		Description: Zinc-Binding Structure of a Catalytic Amyloid from Solid-State NMR Spectroscopy
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
		+	[[Category: Degrado, W.F]]
		+	[[Category: Korendovych, I.V]]
	[[Category: Wang, T]]		[[Category: Wang, T]]
		+	[[Category: Makhlynets, O.V]]
	[[Category: Polizzi, N]]		[[Category: Polizzi, N]]
	[[Category: Wu, Y]]		[[Category: Wu, Y]]
		+	[[Category: Gosavi, P.M]]
	[[Category: Hong, M]]		[[Category: Hong, M]]
	[[Category: Wu, H]]		[[Category: Wu, H]]
-	[[Category: Degrado, W.F]]
-	[[Category: Korendovych, I.V]]
-	[[Category: Makhlynets, O.V]]
-	[[Category: Gosavi, P.M]]
	[[Category: Lee, M]]		[[Category: Lee, M]]

OCA at 14:27, 9 March 2017

OCA — Thu, 09 Mar 2017 14:27:30 GMT

←Older revision		Revision as of 14:27, 9 March 2017
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5ugk is ON HOLD	+	The entry 5ugk is ON HOLD until Paper Publication

	Authors: Lee, M., Wang, T., Makhlynets, O.V., Wu, Y., Polizzi, N., Wu, H., Gosavi, P.M., Korendovych, I.V., DeGrado, W.F., Hong, M.		Authors: Lee, M., Wang, T., Makhlynets, O.V., Wu, Y., Polizzi, N., Wu, H., Gosavi, P.M., Korendovych, I.V., DeGrado, W.F., Hong, M.

OCA at 21:56, 25 January 2017

OCA — Wed, 25 Jan 2017 21:56:35 GMT

←Older revision		Revision as of 21:56, 25 January 2017
Line 3:		Line 3:
	The entry 5ugk is ON HOLD		The entry 5ugk is ON HOLD

-	Authors: ~~Myungwoon~~ Lee, ~~Tuo~~ Wang, ~~Olga V~~. Makhlynets, ~~Yibing~~ Wu, ~~Nicholas~~ Polizzi, ~~Haifan~~ Wu, ~~Pallavi M~~. Gosavi, ~~Ivan V~~. Korendovych, ~~William F~~. DeGrado, ~~Mei~~ Hong	+	Authors: Lee, M., Wang, T., Makhlynets, O.V., Wu, Y., Polizzi, N., Wu, H., Gosavi, P.M., Korendovych, I.V., DeGrado, W.F., Hong, M.

	Description: Zinc-Binding Structure of a Catalytic Amyloid from Solid-State NMR Spectroscopy		Description: Zinc-Binding Structure of a Catalytic Amyloid from Solid-State NMR Spectroscopy
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: ~~Myungwoon Lee, Tuo~~ Wang, ~~Olga V. Makhlynets~~, ~~Yibing~~ Wu, ~~Nicholas Polizzi~~, ~~Haifan~~ Wu, ~~Pallavi M~~. ~~Gosavi~~, ~~Ivan~~ V. ~~Korendovych~~, ~~William F~~. ~~Degrado~~, ~~Mei Hong~~]]	+	[[Category: Wang, T]]
		+	[[Category: Polizzi, N]]
		+	[[Category: Wu, Y]]
		+	[[Category: Hong, M]]
		+	[[Category: Wu, H]]
		+	[[Category: Degrado, W.F]]
		+	[[Category: Korendovych, I.V]]
		+	[[Category: Makhlynets, O.V]]
		+	[[Category: Gosavi, P.M]]
		+	[[Category: Lee, M]]

OCA: Protected "5ugk" [edit=sysop:move=sysop]

OCA — Wed, 18 Jan 2017 14:57:49 GMT

Protected "5ugk" [edit=sysop:move=sysop]

←Older revision

Revision as of 14:57, 18 January 2017

OCA: New page: '''Unreleased structure''' The entry 5ugk is ON HOLD Authors: Myungwoon Lee, Tuo Wang, Olga V. Makhlynets, Yibing Wu, Nicholas Polizzi, Haifan Wu, Pallavi M. Gosavi, Ivan V. Korendovych...

OCA — Wed, 18 Jan 2017 14:57:48 GMT

New page: '''Unreleased structure''' The entry 5ugk is ON HOLD Authors: Myungwoon Lee, Tuo Wang, Olga V. Makhlynets, Yibing Wu, Nicholas Polizzi, Haifan Wu, Pallavi M. Gosavi, Ivan V. Korendovych...

New page

'''Unreleased structure'''

The entry 5ugk is ON HOLD

Authors: Myungwoon Lee, Tuo Wang, Olga V. Makhlynets, Yibing Wu, Nicholas Polizzi, Haifan Wu, Pallavi M. Gosavi, Ivan V. Korendovych, William F. DeGrado, Mei Hong

Description: Zinc-Binding Structure of a Catalytic Amyloid from Solid-State NMR Spectroscopy
[[Category: Unreleased Structures]]
[[Category: Myungwoon Lee, Tuo Wang, Olga V. Makhlynets, Yibing Wu, Nicholas Polizzi, Haifan Wu, Pallavi M. Gosavi, Ivan V. Korendovych, William F. Degrado, Mei Hong]]