5us5 - Revision history

OCA at 10:27, 14 June 2023

OCA — Wed, 14 Jun 2023 10:27:15 GMT

←Older revision		Revision as of 10:27, 14 June 2023
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	==Solution structure of the IreB homodimer==		==Solution structure of the IreB homodimer==
-	<StructureSection load='5us5' size='340' side='right'caption='[[5us5~~]], [[NMR_Ensembles_of_Models \| 20 NMR models~~]]' scene=''>	+	<StructureSection load='5us5' size='340' side='right'caption='[[5us5]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5us5]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Entfa Entfa~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5US5 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5US5 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5us5]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterococcus_faecalis_V583 Enterococcus faecalis V583]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5US5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5US5 FirstGlance]. <br>
-	</td></tr>~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">EF_1202 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=226185 ENTFA])</td></tr>~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5us5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5us5 OCA], [https://pdbe.org/5us5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5us5 RCSB], [https://www.ebi.ac.uk/pdbsum/5us5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5us5 ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5us5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5us5 OCA], [~~http~~://pdbe.org/5us5 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5us5 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5us5 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5us5 ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Y1202_ENTFA Y1202_ENTFA]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 20:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Entfa~~]]	+	[[Category: Enterococcus faecalis V583]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: Structural genomic]]~~	+	[[Category: Kristich CJ]]
-	[[Category: Kristich~~, C J~~]]	+	[[Category: Lytle BL]]
-	[[Category: Lytle~~, B L~~]]	+	[[Category: Peterson FC]]
-	[[Category: Peterson~~, F C~~]]	+	[[Category: Volkman BF]]
-	[[Category: Volkman~~, B F]]~~	+
-	~~[[Category: Cesg]]~~	+
-	~~[[Category: Dimer]]~~	+
-	~~[[Category: Ireb]]~~	+
-	~~[[Category: PSI, Protein structure initiative]]~~	+
-	~~[[Category: Structure from molmol~~]]	+

OCA at 17:11, 11 December 2019

OCA — Wed, 11 Dec 2019 17:11:22 GMT

←Older revision		Revision as of 17:11, 11 December 2019
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	==Solution structure of the IreB homodimer==		==Solution structure of the IreB homodimer==
-	<StructureSection load='5us5' size='340' side='right' caption='[[5us5]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>	+	<StructureSection load='5us5' size='340' side='right'caption='[[5us5]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5us5]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Entfa Entfa]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5US5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5US5 FirstGlance]. <br>		<table><tr><td colspan='2'>[[5us5]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Entfa Entfa]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5US5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5US5 FirstGlance]. <br>
Line 21:		Line 21:
	</StructureSection>		</StructureSection>
	[[Category: Entfa]]		[[Category: Entfa]]
		+	[[Category: Large Structures]]
	[[Category: Structural genomic]]		[[Category: Structural genomic]]
	[[Category: Kristich, C J]]		[[Category: Kristich, C J]]

OCA at 06:10, 16 November 2017

OCA — Thu, 16 Nov 2017 06:10:02 GMT

←Older revision		Revision as of 06:10, 16 November 2017
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-	'''Unreleased structure'''

-	~~The entry~~ 5us5 is ~~ON HOLD until Paper~~ Publication	+	==Solution structure of the IreB homodimer==
		+	<StructureSection load='5us5' size='340' side='right' caption='[[5us5]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5us5]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Entfa Entfa]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5US5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5US5 FirstGlance]. <br>
		+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">EF_1202 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=226185 ENTFA])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5us5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5us5 OCA], [http://pdbe.org/5us5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5us5 RCSB], [http://www.ebi.ac.uk/pdbsum/5us5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5us5 ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Enterococcus faecalis, a leading cause of hospital-acquired infections, exhibits intrinsic resistance to most cephalosporins, which are antibiotics in the beta-lactam family that target cell-wall biosynthesis. A comprehensive understanding of the underlying genetic and biochemical mechanisms of cephalosporin resistance in E. faecalis is lacking. We previously determined that a transmembrane serine/threonine kinase (IreK) and its cognate phosphatase (IreP) reciprocally regulate cephalosporin resistance in E. faecalis, dependent on the kinase activity of IreK. Other than IreK itself, thus far the only known substrate for reversible phosphorylation by IreK and IreP is IreB, a small protein of unknown function that is well conserved in low-GC Gram-positive bacteria. We previously showed that IreB acts as a negative regulator of cephalosporin resistance in E. faecalis. However, the biochemical mechanism by which IreB modulates cephalosporin resistance remains unknown. As a next step toward an understanding of the mechanism by which IreB regulates resistance, we initiated a structure-function study on IreB. The NMR solution structure of IreB was determined, revealing that IreB adopts a unique fold and forms a dimer in vitro. Dimerization of IreB was confirmed in vivo. Substitutions at the dimer interface impaired IreB function and stability in vivo, indicating that dimerization is functionally important for the biological activity of IreB. Hence, these studies provide new insights into the structure and function of a widely conserved protein of unknown function that is an important regulator of antimicrobial resistance in E. faecalis.

-	~~Authors: Lytle~~, ~~B.L~~., ~~Peterson~~, ~~F.C.~~, ~~Volkman~~, ~~B.F.~~, ~~Kristich~~, C.J.~~, Center for Eukaryotic Structural Genomics~~ (~~CESG~~)	+	Structure and Dimerization of IreB, a Negative Regulator of Cephalosporin Resistance in Enterococcus faecalis.,Hall CL, Lytle BL, Jensen D, Hoff JS, Peterson FC, Volkman BF, Kristich CJ J Mol Biol. 2017 Jul 21;429(15):2324-2336. doi: 10.1016/j.jmb.2017.05.019. Epub, 2017 May 24. PMID:28551334<ref>PMID:28551334</ref>

-	~~Description: Solution structure~~ of the ~~IreB homodimer~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
-	[[Category: ~~Lytle, B.L~~]]	+	<div class="pdbe-citations 5us5" style="background-color:#fffaf0;"></div>
-	[[Category: Kristich, C.J]]	+	== References ==
-	[[Category: Peterson, F.C]]	+	<references/>
-	[[Category: ~~Center For Eukaryotic Structural Genomics (~~Cesg)]]	+	__TOC__
-	[[Category: ~~Volkman~~, ~~B.F~~]]	+	</StructureSection>
		+	[[Category: Entfa]]
		+	[[Category: Structural genomic]]
		+	[[Category: Kristich, C J]]
		+	[[Category: Lytle, B L]]
		+	[[Category: Peterson, F C]]
		+	[[Category: Volkman, B F]]
		+	[[Category: Cesg]]
		+	[[Category: Dimer]]
		+	[[Category: Ireb]]
		+	[[Category: PSI, Protein structure initiative]]
		+	[[Category: Structure from molmol]]

OCA at 11:19, 5 April 2017

OCA — Wed, 05 Apr 2017 11:19:00 GMT

←Older revision		Revision as of 11:19, 5 April 2017
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5us5 is ON HOLD	+	The entry 5us5 is ON HOLD until Paper Publication

	Authors: Lytle, B.L., Peterson, F.C., Volkman, B.F., Kristich, C.J., Center for Eukaryotic Structural Genomics (CESG)		Authors: Lytle, B.L., Peterson, F.C., Volkman, B.F., Kristich, C.J., Center for Eukaryotic Structural Genomics (CESG)

OCA at 19:20, 15 March 2017

OCA — Wed, 15 Mar 2017 19:20:17 GMT

←Older revision		Revision as of 19:20, 15 March 2017
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	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Lytle, B.L]]		[[Category: Lytle, B.L]]
		+	[[Category: Kristich, C.J]]
	[[Category: Peterson, F.C]]		[[Category: Peterson, F.C]]
	[[Category: Center For Eukaryotic Structural Genomics (Cesg)]]		[[Category: Center For Eukaryotic Structural Genomics (Cesg)]]
-	[[Category: Kristich, C.J]]
	[[Category: Volkman, B.F]]		[[Category: Volkman, B.F]]

OCA: Protected "5us5" [edit=sysop:move=sysop]

OCA — Thu, 09 Mar 2017 14:51:39 GMT

Protected "5us5" [edit=sysop:move=sysop]

←Older revision

Revision as of 14:51, 9 March 2017

OCA: New page: '''Unreleased structure''' The entry 5us5 is ON HOLD Authors: Lytle, B.L., Peterson, F.C., Volkman, B.F., Kristich, C.J., Center for Eukaryotic Structural Genomics (CESG) Description: ...

OCA — Thu, 09 Mar 2017 14:51:38 GMT

New page: '''Unreleased structure''' The entry 5us5 is ON HOLD Authors: Lytle, B.L., Peterson, F.C., Volkman, B.F., Kristich, C.J., Center for Eukaryotic Structural Genomics (CESG) Description: ...

New page

'''Unreleased structure'''

The entry 5us5 is ON HOLD

Authors: Lytle, B.L., Peterson, F.C., Volkman, B.F., Kristich, C.J., Center for Eukaryotic Structural Genomics (CESG)

Description: Solution structure of the IreB homodimer
[[Category: Unreleased Structures]]
[[Category: Lytle, B.L]]
[[Category: Peterson, F.C]]
[[Category: Center For Eukaryotic Structural Genomics (Cesg)]]
[[Category: Kristich, C.J]]
[[Category: Volkman, B.F]]