5uw8 - Revision history

OCA at 14:29, 6 March 2024

OCA — Wed, 06 Mar 2024 14:29:56 GMT

←Older revision		Revision as of 14:29, 6 March 2024
Line 3:		Line 3:
	<StructureSection load='5uw8' size='340' side='right'caption='[[5uw8]], [[Resolution\|resolution]] 2.15Å' scene=''>		<StructureSection load='5uw8' size='340' side='right'caption='[[5uw8]], [[Resolution\|resolution]] 2.15Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5uw8]] is a 7 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Eco57 Eco57~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UW8 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5UW8 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5uw8]] is a 7 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_O157:H7 Escherichia coli O157:H7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UW8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5UW8 FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~>[[5uvn\|5uvn]], [[5uw2\|5uw2]]</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.15Å</td></tr>
-	~~<tr~~ id~~='gene'><td class~~="~~sblockLbl~~">~~<b>~~[[~~Gene~~\|~~Gene:~~]]~~</b></td><td class="sblockDat">mlaD, Z4556, ECs4072 ([http://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=83334 ECO57])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5uw8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5uw8 OCA], [https://pdbe.org/5uw8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5uw8 RCSB], [https://www.ebi.ac.uk/pdbsum/5uw8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5uw8 ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5uw8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5uw8 OCA], [~~http~~://pdbe.org/5uw8 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5uw8 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5uw8 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5uw8 ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/MLAD_ECO57 MLAD_ECO57]] Part of the ABC transporter complex MlaFEDB that actively prevents phospholipid accumulation at the cell surface. Probably maintains lipid asymmetry in the outer membrane by retrograde trafficking of phospholipids from the outer membrane to the inner membrane.[UniProtKB:P64604]	+	[https://www.uniprot.org/uniprot/MLAD_ECO57 MLAD_ECO57] Part of the ABC transporter complex MlaFEDB that actively prevents phospholipid accumulation at the cell surface. Probably maintains lipid asymmetry in the outer membrane by retrograde trafficking of phospholipids from the outer membrane to the inner membrane.[UniProtKB:P64604]
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	How phospholipids are trafficked between the bacterial inner and outer membranes through the hydrophilic space of the periplasm is not known. We report that members of the mammalian cell entry (MCE) protein family form hexameric assemblies with a central channel capable of mediating lipid transport. The E. coli MCE protein, MlaD, forms a ring associated with an ABC transporter complex in the inner membrane. A soluble lipid-binding protein, MlaC, ferries lipids between MlaD and an outer membrane protein complex. In contrast, EM structures of two other E. coli MCE proteins show that YebT forms an elongated tube consisting of seven stacked MCE rings, and PqiB adopts a syringe-like architecture. Both YebT and PqiB create channels of sufficient length to span the periplasmic space. This work reveals diverse architectures of highly conserved protein-based channels implicated in the transport of lipids between the membranes of bacteria and some eukaryotic organelles.	+
-		+
-	Architectures of Lipid Transport Systems for the Bacterial Outer Membrane.,Ekiert DC, Bhabha G, Isom GL, Greenan G, Ovchinnikov S, Henderson IR, Cox JS, Vale RD Cell. 2017 Apr 6;169(2):273-285.e17. doi: 10.1016/j.cell.2017.03.019. PMID:28388411<ref>PMID:28388411</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 5uw8" style="background-color:#fffaf0;"></div>~~	+
-	~~== References ==~~	+
-	~~<references/>~~	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Eco57~~]]	+	[[Category: Escherichia coli O157:H7]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Bhabha, G]]	+	[[Category: Bhabha G]]
-	[[Category: Ekiert~~, D C]]~~	+	[[Category: Ekiert DC]]
-	~~[[Category: Bacterial lipid transport]]~~	+
-	~~[[Category: Mce protein]]~~	+
-	~~[[Category: Transport protein~~]]	+

OCA at 16:44, 20 November 2019

OCA — Wed, 20 Nov 2019 16:44:18 GMT

←Older revision		Revision as of 16:44, 20 November 2019
Line 1:		Line 1:

	==Structure of E. coli MCE protein MlaD, core MCE domain==		==Structure of E. coli MCE protein MlaD, core MCE domain==
-	<StructureSection load='5uw8' size='340' side='right' caption='[[5uw8]], [[Resolution\|resolution]] 2.15Å' scene=''>	+	<StructureSection load='5uw8' size='340' side='right'caption='[[5uw8]], [[Resolution\|resolution]] 2.15Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5uw8]] is a 7 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UW8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5UW8 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5uw8]] is a 7 chain structure with sequence from [http://en.wikipedia.org/wiki/Eco57 Eco57]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UW8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5UW8 FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5uvn\|5uvn]], [[5uw2\|5uw2]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5uvn\|5uvn]], [[5uw2\|5uw2]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mlaD, Z4556, ECs4072 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83334 ECO57])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5uw8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5uw8 OCA], [http://pdbe.org/5uw8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5uw8 RCSB], [http://www.ebi.ac.uk/pdbsum/5uw8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5uw8 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5uw8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5uw8 OCA], [http://pdbe.org/5uw8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5uw8 RCSB], [http://www.ebi.ac.uk/pdbsum/5uw8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5uw8 ProSAT]</span></td></tr>
	</table>		</table>
Line 22:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Eco57]]
		+	[[Category: Large Structures]]
	[[Category: Bhabha, G]]		[[Category: Bhabha, G]]
	[[Category: Ekiert, D C]]		[[Category: Ekiert, D C]]

OCA at 06:24, 19 April 2017

OCA — Wed, 19 Apr 2017 06:24:57 GMT

←Older revision		Revision as of 06:24, 19 April 2017
Line 9:		Line 9:
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/MLAD_ECO57 MLAD_ECO57]] Part of the ABC transporter complex MlaFEDB that actively prevents phospholipid accumulation at the cell surface. Probably maintains lipid asymmetry in the outer membrane by retrograde trafficking of phospholipids from the outer membrane to the inner membrane.[UniProtKB:P64604]		[[http://www.uniprot.org/uniprot/MLAD_ECO57 MLAD_ECO57]] Part of the ABC transporter complex MlaFEDB that actively prevents phospholipid accumulation at the cell surface. Probably maintains lipid asymmetry in the outer membrane by retrograde trafficking of phospholipids from the outer membrane to the inner membrane.[UniProtKB:P64604]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	How phospholipids are trafficked between the bacterial inner and outer membranes through the hydrophilic space of the periplasm is not known. We report that members of the mammalian cell entry (MCE) protein family form hexameric assemblies with a central channel capable of mediating lipid transport. The E. coli MCE protein, MlaD, forms a ring associated with an ABC transporter complex in the inner membrane. A soluble lipid-binding protein, MlaC, ferries lipids between MlaD and an outer membrane protein complex. In contrast, EM structures of two other E. coli MCE proteins show that YebT forms an elongated tube consisting of seven stacked MCE rings, and PqiB adopts a syringe-like architecture. Both YebT and PqiB create channels of sufficient length to span the periplasmic space. This work reveals diverse architectures of highly conserved protein-based channels implicated in the transport of lipids between the membranes of bacteria and some eukaryotic organelles.
		+
		+	Architectures of Lipid Transport Systems for the Bacterial Outer Membrane.,Ekiert DC, Bhabha G, Isom GL, Greenan G, Ovchinnikov S, Henderson IR, Cox JS, Vale RD Cell. 2017 Apr 6;169(2):273-285.e17. doi: 10.1016/j.cell.2017.03.019. PMID:28388411<ref>PMID:28388411</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5uw8" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 11:48, 12 April 2017

OCA — Wed, 12 Apr 2017 11:48:55 GMT

←Older revision		Revision as of 11:48, 12 April 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry 5uw8 is ON HOLD~~	+	==Structure of E. coli MCE protein MlaD, core MCE domain==
-		+	<StructureSection load='5uw8' size='340' side='right' caption='[[5uw8]], [[Resolution\|resolution]] 2.15Å' scene=''>
-	~~Authors: Bhabha, G., Ekiert, D.C.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5uw8]] is a 7 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UW8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5UW8 FirstGlance]. <br>
-	~~Description:~~ Structure of E. coli MCE protein MlaD, core MCE domain	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5uvn\|5uvn]], [[5uw2\|5uw2]]</td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5uw8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5uw8 OCA], [http://pdbe.org/5uw8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5uw8 RCSB], [http://www.ebi.ac.uk/pdbsum/5uw8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5uw8 ProSAT]</span></td></tr>
-	[[~~Category~~: ~~Ekiert~~, D.C]]	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/MLAD_ECO57 MLAD_ECO57]] Part of the ABC transporter complex MlaFEDB that actively prevents phospholipid accumulation at the cell surface. Probably maintains lipid asymmetry in the outer membrane by retrograde trafficking of phospholipids from the outer membrane to the inner membrane.[UniProtKB:P64604]
		+	__TOC__
		+	</StructureSection>
	[[Category: Bhabha, G]]		[[Category: Bhabha, G]]
		+	[[Category: Ekiert, D C]]
		+	[[Category: Bacterial lipid transport]]
		+	[[Category: Mce protein]]
		+	[[Category: Transport protein]]

OCA: Protected "5uw8" [edit=sysop:move=sysop]

OCA — Thu, 09 Mar 2017 15:00:59 GMT

Protected "5uw8" [edit=sysop:move=sysop]

←Older revision

Revision as of 15:00, 9 March 2017

OCA: New page: '''Unreleased structure''' The entry 5uw8 is ON HOLD Authors: Bhabha, G., Ekiert, D.C. Description: Structure of E. coli MCE protein MlaD, core MCE domain [[Category: Unreleased Struct...

OCA — Thu, 09 Mar 2017 15:00:58 GMT

New page: '''Unreleased structure''' The entry 5uw8 is ON HOLD Authors: Bhabha, G., Ekiert, D.C. Description: Structure of E. coli MCE protein MlaD, core MCE domain [[Category: Unreleased Struct...

New page

'''Unreleased structure'''

The entry 5uw8 is ON HOLD

Authors: Bhabha, G., Ekiert, D.C.

Description: Structure of E. coli MCE protein MlaD, core MCE domain
[[Category: Unreleased Structures]]
[[Category: Ekiert, D.C]]
[[Category: Bhabha, G]]