5uxc - Revision history

OCA at 17:12, 11 December 2019

OCA — Wed, 11 Dec 2019 17:12:39 GMT

←Older revision		Revision as of 17:12, 11 December 2019
Line 1:		Line 1:

	==Crystal structure of macrolide 2'-phosphotransferase MphH from Brachybacterium faecium in complex with GDP==		==Crystal structure of macrolide 2'-phosphotransferase MphH from Brachybacterium faecium in complex with GDP==
-	<StructureSection load='5uxc' size='340' side='right' caption='[[5uxc]], [[Resolution\|resolution]] 1.72Å' scene=''>	+	<StructureSection load='5uxc' size='340' side='right'caption='[[5uxc]], [[Resolution\|resolution]] 1.72Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5uxc]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Brafd Brafd]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UXC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5UXC FirstGlance]. <br>		<table><tr><td colspan='2'>[[5uxc]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Brafd Brafd]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UXC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5UXC FirstGlance]. <br>
Line 24:		Line 24:
	</StructureSection>		</StructureSection>
	[[Category: Brafd]]		[[Category: Brafd]]
		+	[[Category: Large Structures]]
	[[Category: Anderson, W F]]		[[Category: Anderson, W F]]
	[[Category: Structural genomic]]		[[Category: Structural genomic]]

OCA at 08:21, 22 February 2018

OCA — Thu, 22 Feb 2018 08:21:52 GMT

←Older revision		Revision as of 08:21, 22 February 2018
Line 3:		Line 3:
	<StructureSection load='5uxc' size='340' side='right' caption='[[5uxc]], [[Resolution\|resolution]] 1.72Å' scene=''>		<StructureSection load='5uxc' size='340' side='right' caption='[[5uxc]], [[Resolution\|resolution]] 1.72Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5uxc]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UXC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5UXC FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5uxc]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Brafd Brafd]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UXC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5UXC FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZIT:AZITHROMYCIN'>ZIT</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZIT:AZITHROMYCIN'>ZIT</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5uxb\|5uxb]], [[5uxd\|5uxd]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5uxb\|5uxb]], [[5uxd\|5uxd]], [[5uxa\|5uxa]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Bfae_22410 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=446465 BRAFD])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5uxc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5uxc OCA], [http://pdbe.org/5uxc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5uxc RCSB], [http://www.ebi.ac.uk/pdbsum/5uxc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5uxc ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5uxc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5uxc OCA], [http://pdbe.org/5uxc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5uxc RCSB], [http://www.ebi.ac.uk/pdbsum/5uxc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5uxc ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The production of antibiotics by microbes in the environment and their use in medicine and agriculture select for existing and emerging resistance. To address this inevitability, prudent development of antibiotic drugs requires careful consideration of resistance evolution. Here, we identify the molecular basis for expanded substrate specificity in MphI, a macrolide kinase (Mph) that does not confer resistance to erythromycin, in contrast to other known Mphs. Using a combination of phylogenetics, drug-resistance phenotypes, and in vitro enzyme assays, we find that MphI and MphK phosphorylate erythromycin poorly resulting in an antibiotic-sensitive phenotype. Using likelihood reconstruction of ancestral sequences and site-saturation combinatorial mutagenesis, supported by Mph crystal structures, we determine that two non-obvious mutations in combination expand the substrate range. This approach should be applicable for studying the functional evolution of any antibiotic resistance enzyme and for evaluating the evolvability of resistance enzymes to new generations of antibiotic scaffolds.
		+
		+	The evolution of substrate discrimination in macrolide antibiotic resistance enzymes.,Pawlowski AC, Stogios PJ, Koteva K, Skarina T, Evdokimova E, Savchenko A, Wright GD Nat Commun. 2018 Jan 9;9(1):112. doi: 10.1038/s41467-017-02680-0. PMID:29317655<ref>PMID:29317655</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5uxc" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Brafd]]
	[[Category: Anderson, W F]]		[[Category: Anderson, W F]]
	[[Category: Structural genomic]]		[[Category: Structural genomic]]

OCA at 08:25, 6 October 2017

OCA — Fri, 06 Oct 2017 08:25:14 GMT

←Older revision		Revision as of 08:25, 6 October 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5uxc ~~is ON HOLD~~	+	==Crystal structure of macrolide 2'-phosphotransferase MphH from Brachybacterium faecium in complex with GDP==
-		+	<StructureSection load='5uxc' size='340' side='right' caption='[[5uxc]], [[Resolution\|resolution]] 1.72Å' scene=''>
-	~~Authors~~: ~~Stogios, P~~.J., ~~Skarina~~, T., ~~Wawrzak~~, Z., ~~Yim~~, V., ~~Savchenko~~, A., ~~Anderson~~, W.F., ~~Center for Structural Genomics of Infectious Diseases (CSGID)~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5uxc]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UXC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5UXC FirstGlance]. <br>
-	~~Description~~: ~~Crystal structure of macrolide 2'-phosphotransferase MphH from Brachybacterium faecium in complex with GDP~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZIT:AZITHROMYCIN'>ZIT</scene></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5uxb\|5uxb]], [[5uxd\|5uxd]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5uxc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5uxc OCA], [http://pdbe.org/5uxc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5uxc RCSB], [http://www.ebi.ac.uk/pdbsum/5uxc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5uxc ProSAT]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Anderson, W F]]
		+	[[Category: Structural genomic]]
	[[Category: Savchenko, A]]		[[Category: Savchenko, A]]
		+	[[Category: Skarina, T]]
		+	[[Category: Stogios, P J]]
		+	[[Category: Wawrzak, Z]]
	[[Category: Yim, V]]		[[Category: Yim, V]]
-	[[Category: ~~Stogios, P.J~~]]	+	[[Category: Alpha/beta protein]]
-	[[Category: ~~Wawrzak, Z~~]]	+	[[Category: Antibiotic resistance]]
-	[[Category: ~~Skarina, T~~]]	+	[[Category: Azithromycin]]
-	[[Category: ~~Anderson, W.F~~]]	+	[[Category: Cave bacterium]]
-	[[Category: ~~Center For Structural Genomics Of Infectious Diseases (~~Csgid)]]	+	[[Category: Csgid]]
		+	[[Category: Kinase]]
		+	[[Category: Macrolide]]
		+	[[Category: National institute of allergy and infectious disease]]
		+	[[Category: Niaid]]
		+	[[Category: Phosphotransferase]]
		+	[[Category: Transferase-antibiotic complex]]

OCA at 19:28, 15 March 2017

OCA — Wed, 15 Mar 2017 19:28:47 GMT

←Older revision		Revision as of 19:28, 15 March 2017
Line 7:		Line 7:
	Description: Crystal structure of macrolide 2'-phosphotransferase MphH from Brachybacterium faecium in complex with GDP		Description: Crystal structure of macrolide 2'-phosphotransferase MphH from Brachybacterium faecium in complex with GDP
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Wawrzak, Z]]
	[[Category: Savchenko, A]]		[[Category: Savchenko, A]]
	[[Category: Yim, V]]		[[Category: Yim, V]]
	[[Category: Stogios, P.J]]		[[Category: Stogios, P.J]]
		+	[[Category: Wawrzak, Z]]
	[[Category: Skarina, T]]		[[Category: Skarina, T]]
	[[Category: Anderson, W.F]]		[[Category: Anderson, W.F]]
	[[Category: Center For Structural Genomics Of Infectious Diseases (Csgid)]]		[[Category: Center For Structural Genomics Of Infectious Diseases (Csgid)]]

OCA: Protected "5uxc" [edit=sysop:move=sysop]

OCA — Thu, 09 Mar 2017 15:03:32 GMT

Protected "5uxc" [edit=sysop:move=sysop]

←Older revision

Revision as of 15:03, 9 March 2017

OCA: New page: '''Unreleased structure''' The entry 5uxc is ON HOLD Authors: Stogios, P.J., Skarina, T., Wawrzak, Z., Yim, V., Savchenko, A., Anderson, W.F., Center for Structural Genomics of Infectio...

OCA — Thu, 09 Mar 2017 15:03:31 GMT

New page: '''Unreleased structure''' The entry 5uxc is ON HOLD Authors: Stogios, P.J., Skarina, T., Wawrzak, Z., Yim, V., Savchenko, A., Anderson, W.F., Center for Structural Genomics of Infectio...

New page

'''Unreleased structure'''

The entry 5uxc is ON HOLD

Authors: Stogios, P.J., Skarina, T., Wawrzak, Z., Yim, V., Savchenko, A., Anderson, W.F., Center for Structural Genomics of Infectious Diseases (CSGID)

Description: Crystal structure of macrolide 2'-phosphotransferase MphH from Brachybacterium faecium in complex with GDP
[[Category: Unreleased Structures]]
[[Category: Wawrzak, Z]]
[[Category: Savchenko, A]]
[[Category: Yim, V]]
[[Category: Stogios, P.J]]
[[Category: Skarina, T]]
[[Category: Anderson, W.F]]
[[Category: Center For Structural Genomics Of Infectious Diseases (Csgid)]]