5uxo - Revision history

OCA at 16:46, 20 November 2019

OCA — Wed, 20 Nov 2019 16:46:27 GMT

←Older revision		Revision as of 16:46, 20 November 2019
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	==Type II DAH7PS from Pseudomonas aeruginosa==		==Type II DAH7PS from Pseudomonas aeruginosa==
-	<StructureSection load='5uxo' size='340' side='right' caption='[[5uxo]], [[Resolution\|resolution]] 2.35Å' scene=''>	+	<StructureSection load='5uxo' size='340' side='right'caption='[[5uxo]], [[Resolution\|resolution]] 2.35Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5uxo]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseae Pseae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UXO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5UXO FirstGlance]. <br>		<table><tr><td colspan='2'>[[5uxo]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseae Pseae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UXO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5UXO FirstGlance]. <br>
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	</StructureSection>		</StructureSection>
	[[Category: 3-deoxy-7-phosphoheptulonate synthase]]		[[Category: 3-deoxy-7-phosphoheptulonate synthase]]
		+	[[Category: Large Structures]]
	[[Category: Pseae]]		[[Category: Pseae]]
	[[Category: Jameson, G B]]		[[Category: Jameson, G B]]

OCA at 08:25, 2 May 2018

OCA — Wed, 02 May 2018 08:25:47 GMT

←Older revision		Revision as of 08:25, 2 May 2018
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	<StructureSection load='5uxo' size='340' side='right' caption='[[5uxo]], [[Resolution\|resolution]] 2.35Å' scene=''>		<StructureSection load='5uxo' size='340' side='right' caption='[[5uxo]], [[Resolution\|resolution]] 2.35Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5uxo]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UXO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5UXO FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5uxo]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseae Pseae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UXO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5UXO FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=PEP:PHOSPHOENOLPYRUVATE'>PEP</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=PEP:PHOSPHOENOLPYRUVATE'>PEP</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5uxm\|5uxm]], [[5uxn\|5uxn]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5uxm\|5uxm]], [[5uxn\|5uxn]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PA2843 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208964 PSEAE])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/3-deoxy-7-phosphoheptulonate_synthase 3-deoxy-7-phosphoheptulonate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.54 2.5.1.54] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/3-deoxy-7-phosphoheptulonate_synthase 3-deoxy-7-phosphoheptulonate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.54 2.5.1.54] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5uxo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5uxo OCA], [http://pdbe.org/5uxo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5uxo RCSB], [http://www.ebi.ac.uk/pdbsum/5uxo PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5uxo ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5uxo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5uxo OCA], [http://pdbe.org/5uxo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5uxo RCSB], [http://www.ebi.ac.uk/pdbsum/5uxo PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5uxo ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The shikimate pathway is responsible for the biosynthesis of key aromatic metabolites in microorganisms and plants. The enzyme 3-deoxy-d- arabino-heptulosonate 7-phosphate synthase (DAH7PS) catalyzes the first step of the pathway and DAH7PSs are classified as either type I or type II. The DAH7PSs from Pseudomonas aeruginosa are of particular interest as open reading frames encoding four putative DAH7PS isoenzymes, two classified as type Ialpha and two classified as type II, have been identified. Here, the structure of a type II DAH7PS enzyme from P. aeruginosa (PAO1) has been determined at 1.54 A resolution, in complex with its allosteric inhibitor tryptophan. Structural differences in the extra-barrel elements, when compared to other type II DAH7PS enzymes, directly relate to the formation of a distinct quaternary conformation with consequences for allosteric function and the control of flux to branching pathways. In contrast to the well-characterized Mycobacterium tuberculosis type II DAH7PS, which binds multiple allosteric inhibitors, this PaeDAH7PS(PA2843) is observed to be modestly allosterically inhibited by a single aromatic amino acid, tryptophan. In addition, structures in complex with tyrosine or with no allosteric ligand bound were determined. These structures provide new insights into the linkages between the active and allosteric sites. With four putative DAH7PS enzymes, P. aeruginosa appears to have evolved control of shikimate pathway flux at the genetic level, rather than control by multiple allosteric effectors to a single type II DAH7PS, as in M. tuberculosis. Type II DAH7PSs, thus, appear to have a more varied evolutionary trajectory than previously indicated.
		+
		+	A Pseudoisostructural Type II DAH7PS Enzyme from Pseudomonas aeruginosa: Alternative Evolutionary Strategies to Control Shikimate Pathway Flux.,Sterritt OW, Kessans SA, Jameson GB, Parker EJ Biochemistry. 2018 Apr 16. doi: 10.1021/acs.biochem.8b00082. PMID:29608284<ref>PMID:29608284</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5uxo" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: 3-deoxy-7-phosphoheptulonate synthase]]		[[Category: 3-deoxy-7-phosphoheptulonate synthase]]
		+	[[Category: Pseae]]
	[[Category: Jameson, G B]]		[[Category: Jameson, G B]]
	[[Category: Parker, E J]]		[[Category: Parker, E J]]

OCA at 06:24, 28 February 2018

OCA — Wed, 28 Feb 2018 06:24:27 GMT

←Older revision		Revision as of 06:24, 28 February 2018
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-	'''Unreleased structure'''

-	~~The entry~~ 5uxo is ~~ON HOLD until Paper Publication~~	+	==Type II DAH7PS from Pseudomonas aeruginosa==
-		+	<StructureSection load='5uxo' size='340' side='right' caption='[[5uxo]], [[Resolution\|resolution]] 2.35Å' scene=''>
-	~~Authors~~: ~~Sterritt~~, O.W., ~~Jameson~~, G.B., ~~Parker~~, E.J.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5uxo]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UXO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5UXO FirstGlance]. <br>
-	~~Description: Type II DAH7PS from Pseudomonas aeruginosa~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=PEP:PHOSPHOENOLPYRUVATE'>PEP</scene></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5uxm\|5uxm]], [[5uxn\|5uxn]]</td></tr>
-	[[Category: ~~Sterritt~~, ~~O.W~~]]	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/3-deoxy-7-phosphoheptulonate_synthase 3-deoxy-7-phosphoheptulonate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.54 2.5.1.54] </span></td></tr>
-	[[Category: Parker, E.J]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5uxo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5uxo OCA], [http://pdbe.org/5uxo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5uxo RCSB], [http://www.ebi.ac.uk/pdbsum/5uxo PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5uxo ProSAT]</span></td></tr>
-	[[Category: ~~Jameson~~, ~~G.B~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: 3-deoxy-7-phosphoheptulonate synthase]]
		+	[[Category: Jameson, G B]]
		+	[[Category: Parker, E J]]
		+	[[Category: Sterritt, O W]]
		+	[[Category: 3-deoxy-d-arabino-heptulosonate 7-phosphate synthase]]
		+	[[Category: Dah7p]]
		+	[[Category: Shikimate pathway]]
		+	[[Category: Transferase]]

OCA at 13:50, 27 September 2017

OCA — Wed, 27 Sep 2017 13:50:22 GMT

←Older revision		Revision as of 13:50, 27 September 2017
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	Description: Type II DAH7PS from Pseudomonas aeruginosa		Description: Type II DAH7PS from Pseudomonas aeruginosa
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
		+	[[Category: Sterritt, O.W]]
	[[Category: Parker, E.J]]		[[Category: Parker, E.J]]
	[[Category: Jameson, G.B]]		[[Category: Jameson, G.B]]
-	[[Category: Sterritt, O.W]]

OCA at 11:06, 24 May 2017

OCA — Wed, 24 May 2017 11:06:50 GMT

←Older revision		Revision as of 11:06, 24 May 2017
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5uxo is ON HOLD	+	The entry 5uxo is ON HOLD until Paper Publication

	Authors: Sterritt, O.W., Jameson, G.B., Parker, E.J.		Authors: Sterritt, O.W., Jameson, G.B., Parker, E.J.

OCA at 19:29, 15 March 2017

OCA — Wed, 15 Mar 2017 19:29:23 GMT

←Older revision		Revision as of 19:29, 15 March 2017
Line 7:		Line 7:
	Description: Type II DAH7PS from Pseudomonas aeruginosa		Description: Type II DAH7PS from Pseudomonas aeruginosa
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Sterritt, O.W]]
	[[Category: Parker, E.J]]		[[Category: Parker, E.J]]
	[[Category: Jameson, G.B]]		[[Category: Jameson, G.B]]
		+	[[Category: Sterritt, O.W]]

OCA: Protected "5uxo" [edit=sysop:move=sysop]

OCA — Thu, 09 Mar 2017 15:04:17 GMT

Protected "5uxo" [edit=sysop:move=sysop]

←Older revision

Revision as of 15:04, 9 March 2017

OCA: New page: '''Unreleased structure''' The entry 5uxo is ON HOLD Authors: Sterritt, O.W., Jameson, G.B., Parker, E.J. Description: Type II DAH7PS from Pseudomonas aeruginosa [[Category: Unreleased...

OCA — Thu, 09 Mar 2017 15:04:16 GMT

New page: '''Unreleased structure''' The entry 5uxo is ON HOLD Authors: Sterritt, O.W., Jameson, G.B., Parker, E.J. Description: Type II DAH7PS from Pseudomonas aeruginosa [[Category: Unreleased...

New page

'''Unreleased structure'''

The entry 5uxo is ON HOLD

Authors: Sterritt, O.W., Jameson, G.B., Parker, E.J.

Description: Type II DAH7PS from Pseudomonas aeruginosa
[[Category: Unreleased Structures]]
[[Category: Sterritt, O.W]]
[[Category: Parker, E.J]]
[[Category: Jameson, G.B]]