5v11 - Revision history

OCA at 11:24, 13 November 2019

OCA — Wed, 13 Nov 2019 11:24:45 GMT

←Older revision		Revision as of 11:24, 13 November 2019
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	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5v11 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5v11 OCA], [http://pdbe.org/5v11 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5v11 RCSB], [http://www.ebi.ac.uk/pdbsum/5v11 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5v11 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5v11 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5v11 OCA], [http://pdbe.org/5v11 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5v11 RCSB], [http://www.ebi.ac.uk/pdbsum/5v11 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5v11 ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The lipid composition of the cellular membrane plays an important role in a number of biological processes including the binding of membrane-active peptides. Characterization of membrane binding remains challenging, due to the technical limitations associated with the use of standard biophysical techniques and available membrane models. Here, we investigate the lipid binding properties of two membrane-active peptides, VSTx1, a well characterized ion-channel inhibitor, identified from spider venom, that preferentially binds to anionic lipid mixtures, and AA139 an antimicrobial beta-hairpin peptide with uncharacterised lipid binding properties, currently in pre-clinical development. The lipid binding properties of these peptides are elucidated using nanodiscs formed by both linear and circularized (sortase-mediated) forms of a membrane scaffold protein (MSP1D1DeltaH5). We find that nanodiscs formed by circularized MSPs-in contrast to those formed by linear MSPs-are sufficiently stable under sample conditions typically used for biophysical measurements (including lipid composition, a range of buffers, temperatures and concentrations). Using these circularized nanodiscs, we are able to extract detailed thermodynamic data using isothermal titration calorimetry (ITC) as well as atomic resolution mapping of the lipid binding interfaces of our isotope labeled peptides using solution-state, heteronuclear, nuclear magnetic resonance (NMR) spectroscopy. This represents a novel and general approach for elucidating the thermodynamics and molecular interface of membrane-active peptides toward flat lipid bilayers of variable composition. Our approach is validated by first determining the thermodynamic parameters and binding interface of VSTx1 toward the lipid bilayer, which shows good agreement with previous studies using lipid micelles and liposomes. The method is then applied to AA139, where the membrane binding properties are unknown. This characterization, involved solving the high-resolution structure of AA139 in solution using NMR spectroscopy and the development of a suitable expression system for isotope labeling. AA139 was found to bind exclusively to anionic membranes with moderate affinity (K d~low muM), and was found to have a lipid binding interface involving the termini of the beta-hairpin structure. The preference of AA139 for anionic lipids supports a role for membrane binding in the mode-of-action of this peptide, which is also consistent with its higher inhibitory activity against bacterial cells compared to mammalian cells. The described approach is a powerful method for investigation of the membrane binding properties of this important class of molecules.
		+
		+	Elucidating the Lipid Binding Properties of Membrane-Active Peptides Using Cyclised Nanodiscs.,Zhang AH, Edwards IA, Mishra BP, Sharma G, Healy MD, Elliott AG, Blaskovich MAT, Cooper MA, Collins BM, Jia X, Mobli M Front Chem. 2019 Apr 16;7:238. doi: 10.3389/fchem.2019.00238. eCollection 2019. PMID:31058133<ref>PMID:31058133</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5v11" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 06:28, 17 April 2019

OCA — Wed, 17 Apr 2019 06:28:58 GMT

←Older revision		Revision as of 06:28, 17 April 2019
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	==Solution structure of arenicin-3 synthetic analog.==		==Solution structure of arenicin-3 synthetic analog.==
-	<StructureSection load='5v11' size='340' side='right' caption='[[5v11]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>	+	<StructureSection load='5v11' size='340' side='right'caption='[[5v11]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5v11]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5V11 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5V11 FirstGlance]. <br>		<table><tr><td colspan='2'>[[5v11]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5V11 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5V11 FirstGlance]. <br>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Edwards, I A]]		[[Category: Edwards, I A]]
	[[Category: Mobli, M]]		[[Category: Mobli, M]]

OCA at 21:39, 9 August 2018

OCA — Thu, 09 Aug 2018 21:39:43 GMT

←Older revision		Revision as of 21:39, 9 August 2018
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-	'''Unreleased structure'''

-	~~The entry~~ 5v11 ~~is ON HOLD until Aug 29 2019~~	+	==Solution structure of arenicin-3 synthetic analog.==
-		+	<StructureSection load='5v11' size='340' side='right' caption='[[5v11]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
-	~~Authors~~: ~~Edwards, I~~.A.~~, Mobli, M~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5v11]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5V11 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5V11 FirstGlance]. <br>
-	~~Description~~: ~~Solution structure of arenicin~~-~~3 synthetic analog~~.	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5u0y\|5u0y]]</td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5v11 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5v11 OCA], [http://pdbe.org/5v11 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5v11 RCSB], [http://www.ebi.ac.uk/pdbsum/5v11 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5v11 ProSAT]</span></td></tr>
-	[[Category: Edwards, I.A]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Edwards, I A]]
	[[Category: Mobli, M]]		[[Category: Mobli, M]]
		+	[[Category: Antimicrobial peptide]]
		+	[[Category: Antimicrobial protein]]
		+	[[Category: B-hairpin]]

OCA at 03:59, 18 April 2018

OCA — Wed, 18 Apr 2018 03:59:35 GMT

←Older revision		Revision as of 03:59, 18 April 2018
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5v11 is ON HOLD until ~~Paper Publication~~	+	The entry 5v11 is ON HOLD until Aug 29 2019

	Authors: Edwards, I.A., Mobli, M.		Authors: Edwards, I.A., Mobli, M.
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	Description: Solution structure of arenicin-3 synthetic analog.		Description: Solution structure of arenicin-3 synthetic analog.
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Mobli, M]]
	[[Category: Edwards, I.A]]		[[Category: Edwards, I.A]]
		+	[[Category: Mobli, M]]

OCA at 04:47, 20 December 2017

OCA — Wed, 20 Dec 2017 04:47:36 GMT

←Older revision		Revision as of 04:47, 20 December 2017
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5v11 is ON HOLD	+	The entry 5v11 is ON HOLD until Paper Publication

	Authors: Edwards, I.A., Mobli, M.		Authors: Edwards, I.A., Mobli, M.

OCA: Protected "5v11" [edit=sysop:move=sysop]

OCA — Thu, 09 Mar 2017 15:12:36 GMT

Protected "5v11" [edit=sysop:move=sysop]

←Older revision

Revision as of 15:12, 9 March 2017

OCA: New page: '''Unreleased structure''' The entry 5v11 is ON HOLD Authors: Edwards, I.A., Mobli, M. Description: Solution structure of arenicin-3 synthetic analog. [[Category: Unreleased Structures...

OCA — Thu, 09 Mar 2017 15:12:35 GMT

New page: '''Unreleased structure''' The entry 5v11 is ON HOLD Authors: Edwards, I.A., Mobli, M. Description: Solution structure of arenicin-3 synthetic analog. [[Category: Unreleased Structures...

New page

'''Unreleased structure'''

The entry 5v11 is ON HOLD

Authors: Edwards, I.A., Mobli, M.

Description: Solution structure of arenicin-3 synthetic analog.
[[Category: Unreleased Structures]]
[[Category: Mobli, M]]
[[Category: Edwards, I.A]]