5vaw - Revision history

OCA at 13:45, 4 October 2023

OCA — Wed, 04 Oct 2023 13:45:04 GMT

←Older revision		Revision as of 13:45, 4 October 2023
Line 3:		Line 3:
	<StructureSection load='5vaw' size='340' side='right'caption='[[5vaw]], [[Resolution\|resolution]] 1.69Å' scene=''>		<StructureSection load='5vaw' size='340' side='right'caption='[[5vaw]], [[Resolution\|resolution]] 1.69Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5vaw]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Aciba Aciba~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VAW OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5VAW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5vaw]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Acinetobacter_baumannii Acinetobacter baumannii] and [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VAW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5VAW FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GLU:GLUTAMIC+ACID'>GLU</scene>, <scene name='pdbligand=LYS:LYSINE'>LYS</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SER:SERINE'>SER</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.69Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5ihj\|5ihj]], [[5cfv\|5cfv]], [[4xa2\|4xa2]]~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GLU:GLUTAMIC+ACID'>GLU</scene>, <scene name='pdbligand=LYS:LYSINE'>LYS</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SER:SERINE'>SER</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5vaw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5vaw OCA], [~~http~~://pdbe.org/5vaw PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5vaw RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5vaw PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5vaw ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5vaw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5vaw OCA], [https://pdbe.org/5vaw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5vaw RCSB], [https://www.ebi.ac.uk/pdbsum/5vaw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5vaw ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/MALE_ECOLI MALE_ECOLI] Involved in the high-affinity maltose membrane transport system MalEFGK. Initial receptor for the active transport of and chemotaxis toward maltooligosaccharides.[https://www.uniprot.org/uniprot/A0A0D5YCX7_ACIBA A0A0D5YCX7_ACIBA]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Aciba~~]]	+	[[Category: Acinetobacter baumannii]]
		+	[[Category: Escherichia coli]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Piepenbrink~~, K H~~]]	+	[[Category: Piepenbrink KH]]
-	[[Category: Sundberg~~, E J]]~~	+	[[Category: Sundberg EJ]]
-	~~[[Category: Cell adhesion]]~~	+
-	~~[[Category: Type iv pilin~~]]	+

OCA at 09:09, 21 October 2020

OCA — Wed, 21 Oct 2020 09:09:45 GMT

←Older revision		Revision as of 09:09, 21 October 2020
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	==Fusion of Maltose-binding Protein and PilA from Acinetobacter baumannii AB5075==		==Fusion of Maltose-binding Protein and PilA from Acinetobacter baumannii AB5075==
-	<StructureSection load='5vaw' size='340' side='right' caption='[[5vaw]], [[Resolution\|resolution]] 1.69Å' scene=''>	+	<StructureSection load='5vaw' size='340' side='right'caption='[[5vaw]], [[Resolution\|resolution]] 1.69Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5vaw]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Aciba Aciba]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VAW OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5VAW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5vaw]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Aciba Aciba]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VAW OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5VAW FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GLU:GLUTAMIC+ACID'>GLU</scene>, <scene name='pdbligand=LYS:LYSINE'>LYS</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SER:SERINE'>SER</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GLU:GLUTAMIC+ACID'>GLU</scene>, <scene name='pdbligand=LYS:LYSINE'>LYS</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SER:SERINE'>SER</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5ihj\|5ihj]], [[5cfv\|5cfv]], [[4xa2\|4xa2]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5ihj\|5ihj]], [[5cfv\|5cfv]], [[4xa2\|4xa2]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5vaw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5vaw OCA], [http://pdbe.org/5vaw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5vaw RCSB], [http://www.ebi.ac.uk/pdbsum/5vaw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5vaw ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5vaw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5vaw OCA], [http://pdbe.org/5vaw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5vaw RCSB], [http://www.ebi.ac.uk/pdbsum/5vaw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5vaw ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 22:		Line 22:
	</StructureSection>		</StructureSection>
	[[Category: Aciba]]		[[Category: Aciba]]
		+	[[Category: Large Structures]]
	[[Category: Piepenbrink, K H]]		[[Category: Piepenbrink, K H]]
	[[Category: Sundberg, E J]]		[[Category: Sundberg, E J]]
	[[Category: Cell adhesion]]		[[Category: Cell adhesion]]
	[[Category: Type iv pilin]]		[[Category: Type iv pilin]]

OCA at 20:46, 2 December 2018

OCA — Sun, 02 Dec 2018 20:46:52 GMT

←Older revision		Revision as of 20:46, 2 December 2018
Line 3:		Line 3:
	<StructureSection load='5vaw' size='340' side='right' caption='[[5vaw]], [[Resolution\|resolution]] 1.69Å' scene=''>		<StructureSection load='5vaw' size='340' side='right' caption='[[5vaw]], [[Resolution\|resolution]] 1.69Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5vaw]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VAW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5VAW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5vaw]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Aciba Aciba]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VAW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5VAW FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GLU:GLUTAMIC+ACID'>GLU</scene>, <scene name='pdbligand=LYS:LYSINE'>LYS</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SER:SERINE'>SER</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GLU:GLUTAMIC+ACID'>GLU</scene>, <scene name='pdbligand=LYS:LYSINE'>LYS</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SER:SERINE'>SER</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5ihj\|5ihj]], [[5cfv\|5cfv]], [[4xa2\|4xa2]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5ihj\|5ihj]], [[5cfv\|5cfv]], [[4xa2\|4xa2]]</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5vaw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5vaw OCA], [http://pdbe.org/5vaw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5vaw RCSB], [http://www.ebi.ac.uk/pdbsum/5vaw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5vaw ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5vaw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5vaw OCA], [http://pdbe.org/5vaw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5vaw RCSB], [http://www.ebi.ac.uk/pdbsum/5vaw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5vaw ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Type IV pili (T4P) are bacterial appendages composed of protein subunits, called pilins, non-covalently assembled into helical fibers. T4P are essential, in many bacterial species, for processes as diverse as twitching motility, natural competence, biofilm or microcolony formation, and host-cell adhesion. The genes encoding type IV pili are found universally in the Gram-negative, aerobic, non-flagellated, and pathogenic coccobacillus Acinetobacter baumannii, but there is considerable variation in PilA, the major protein subunit, both in amino acid sequence and glycosylation patterns. Here we report the X-tray crystal structure of PilA from AB5075, a recently-characterized, highly-virulent isolate, at 1.9A resolution and compare it to homologues from A. baumannii strains ACICU and BIDMC57, which are C-terminally-glycosylated. These structural comparisons revealed that PilA(AB5075) exhibits a distinctly electronegative surface chemistry. To understand the functional consequences of this change in surface electrostatics, we complemented a DeltapilA knockout strain with divergent pilA genes from ACICU, BIDMC57 and AB5075. The resulting transgenic strains showed differential twitching motility and biofilm formation while maintaining the ability to adhere to epithelial cells. PilA(AB5075) and PilA(ACICU), while structurally similar, promote different characteristics, favoring twitching motility and biofilm formation respectively. These results support a model in which differences in pilus electrostatics affect the equilibrium of microcolony formation, which in turn alters the balance between motility and biofilm formation in Acinetobacter.
		+
		+	The Structure of PilA from Acinetobacter baumannii AB5075 suggests a mechanism for functional specialization in Acinetobacter type IV pili.,Ronish LA, Lillehoj E, Fields JK, Sundberg EJ, Piepenbrink KH J Biol Chem. 2018 Nov 9. pii: RA118.005814. doi: 10.1074/jbc.RA118.005814. PMID:30413536<ref>PMID:30413536</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5vaw" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Aciba]]
	[[Category: Piepenbrink, K H]]		[[Category: Piepenbrink, K H]]
	[[Category: Sundberg, E J]]		[[Category: Sundberg, E J]]
	[[Category: Cell adhesion]]		[[Category: Cell adhesion]]
	[[Category: Type iv pilin]]		[[Category: Type iv pilin]]

OCA at 06:31, 28 March 2018

OCA — Wed, 28 Mar 2018 06:31:31 GMT

←Older revision		Revision as of 06:31, 28 March 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry 5vaw is ON HOLD until Paper Publication~~	+	==Fusion of Maltose-binding Protein and PilA from Acinetobacter baumannii AB5075==
-		+	<StructureSection load='5vaw' size='340' side='right' caption='[[5vaw]], [[Resolution\|resolution]] 1.69Å' scene=''>
-	~~Authors: Piepenbrink, K.H., Sundberg, E.J.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5vaw]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VAW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5VAW FirstGlance]. <br>
-	~~Description:~~ Fusion of Maltose-binding Protein and PilA from Acinetobacter baumannii AB5075	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GLU:GLUTAMIC+ACID'>GLU</scene>, <scene name='pdbligand=LYS:LYSINE'>LYS</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SER:SERINE'>SER</scene></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5ihj\|5ihj]], [[5cfv\|5cfv]], [[4xa2\|4xa2]]</td></tr>
-	[[Category: Piepenbrink, K.H]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5vaw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5vaw OCA], [http://pdbe.org/5vaw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5vaw RCSB], [http://www.ebi.ac.uk/pdbsum/5vaw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5vaw ProSAT]</span></td></tr>
-	[[Category: Sundberg, E.J]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Piepenbrink, K H]]
		+	[[Category: Sundberg, E J]]
		+	[[Category: Cell adhesion]]
		+	[[Category: Type iv pilin]]

OCA at 13:58, 27 September 2017

OCA — Wed, 27 Sep 2017 13:58:16 GMT

←Older revision		Revision as of 13:58, 27 September 2017
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5vaw is ON HOLD	+	The entry 5vaw is ON HOLD until Paper Publication

	Authors: Piepenbrink, K.H., Sundberg, E.J.		Authors: Piepenbrink, K.H., Sundberg, E.J.

OCA at 11:14, 19 April 2017

OCA — Wed, 19 Apr 2017 11:14:46 GMT

←Older revision		Revision as of 11:14, 19 April 2017
Line 3:		Line 3:
	The entry 5vaw is ON HOLD		The entry 5vaw is ON HOLD

-	Authors: Piepenbrink, KH, Sundberg, EJ	+	Authors: Piepenbrink, K.H., Sundberg, E.J.

	Description: Fusion of Maltose-binding Protein and PilA from Acinetobacter baumannii AB5075		Description: Fusion of Maltose-binding Protein and PilA from Acinetobacter baumannii AB5075
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Piepenbrink, ~~Kh,~~ Sundberg, Ej]]	+	[[Category: Piepenbrink, K.H]]
		+	[[Category: Sundberg, E.J]]

OCA: Protected "5vaw" [edit=sysop:move=sysop]

OCA — Wed, 05 Apr 2017 11:52:32 GMT

Protected "5vaw" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:52, 5 April 2017

OCA: New page: '''Unreleased structure''' The entry 5vaw is ON HOLD Authors: Piepenbrink, KH, Sundberg, EJ Description: Fusion of Maltose-binding Protein and PilA from Acinetobacter baumannii AB5075 ...

OCA — Wed, 05 Apr 2017 11:52:31 GMT

New page: '''Unreleased structure''' The entry 5vaw is ON HOLD Authors: Piepenbrink, KH, Sundberg, EJ Description: Fusion of Maltose-binding Protein and PilA from Acinetobacter baumannii AB5075 ...

New page

'''Unreleased structure'''

The entry 5vaw is ON HOLD

Authors: Piepenbrink, KH, Sundberg, EJ

Description: Fusion of Maltose-binding Protein and PilA from Acinetobacter baumannii AB5075
[[Category: Unreleased Structures]]
[[Category: Piepenbrink, Kh, Sundberg, Ej]]