5vl9 - Revision history

OCA at 07:07, 16 March 2022

OCA — Wed, 16 Mar 2022 07:07:14 GMT

←Older revision		Revision as of 07:07, 16 March 2022
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	<StructureSection load='5vl9' size='340' side='right'caption='[[5vl9]], [[Resolution\|resolution]] 2.16Å' scene=''>		<StructureSection load='5vl9' size='340' side='right'caption='[[5vl9]], [[Resolution\|resolution]] 2.16Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5vl9]] is a 8 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"enterobacter_lignolyticus"_deangelis_et_al._2011 "enterobacter lignolyticus" deangelis et al. 2011]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VL9 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5VL9 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5vl9]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/"enterobacter_lignolyticus"_deangelis_et_al._2011 "enterobacter lignolyticus" deangelis et al. 2011]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VL9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5VL9 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEZ:HEXANE-1,6-DIOL'>HEZ</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEZ:HEXANE-1,6-DIOL'>HEZ</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5vlm\|5vlm]], [[5vlg\|5vlg]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[5vlm\|5vlm]], [[5vlg\|5vlg]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Entcl_2353 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1334193 "Enterobacter lignolyticus" Deangelis et al. 2011])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Entcl_2353 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1334193 "Enterobacter lignolyticus" Deangelis et al. 2011])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5vl9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5vl9 OCA], [~~http~~://pdbe.org/5vl9 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5vl9 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5vl9 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5vl9 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5vl9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5vl9 OCA], [https://pdbe.org/5vl9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5vl9 RCSB], [https://www.ebi.ac.uk/pdbsum/5vl9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5vl9 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	</div>		</div>
	<div class="pdbe-citations 5vl9" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5vl9" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Tetracycline repressor protein 3D structures\|Tetracycline repressor protein 3D structures]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 10:15, 26 February 2020

OCA — Wed, 26 Feb 2020 10:15:15 GMT

←Older revision		Revision as of 10:15, 26 February 2020
Line 1:		Line 1:

	==Crystal structure of EilR in complex with eilO DNA element==		==Crystal structure of EilR in complex with eilO DNA element==
-	<StructureSection load='5vl9' size='340' side='right' caption='[[5vl9]], [[Resolution\|resolution]] 2.16Å' scene=''>	+	<StructureSection load='5vl9' size='340' side='right'caption='[[5vl9]], [[Resolution\|resolution]] 2.16Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5vl9]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/"enterobacter_lignolyticus"_deangelis_et_al._2011 "enterobacter lignolyticus" deangelis et al. 2011]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VL9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5VL9 FirstGlance]. <br>		<table><tr><td colspan='2'>[[5vl9]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/"enterobacter_lignolyticus"_deangelis_et_al._2011 "enterobacter lignolyticus" deangelis et al. 2011]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VL9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5VL9 FirstGlance]. <br>
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	</StructureSection>		</StructureSection>
	[[Category: Enterobacter lignolyticus deangelis et al. 2011]]		[[Category: Enterobacter lignolyticus deangelis et al. 2011]]
		+	[[Category: Large Structures]]
	[[Category: Adams, P D]]		[[Category: Adams, P D]]
	[[Category: Chen, J]]		[[Category: Chen, J]]

OCA at 20:47, 2 December 2018

OCA — Sun, 02 Dec 2018 20:47:14 GMT

←Older revision		Revision as of 20:47, 2 December 2018
Line 3:		Line 3:
	<StructureSection load='5vl9' size='340' side='right' caption='[[5vl9]], [[Resolution\|resolution]] 2.16Å' scene=''>		<StructureSection load='5vl9' size='340' side='right' caption='[[5vl9]], [[Resolution\|resolution]] 2.16Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5vl9]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VL9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5VL9 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5vl9]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/"enterobacter_lignolyticus"_deangelis_et_al._2011 "enterobacter lignolyticus" deangelis et al. 2011]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VL9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5VL9 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEZ:HEXANE-1,6-DIOL'>HEZ</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEZ:HEXANE-1,6-DIOL'>HEZ</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5vlm\|5vlm]], [[5vlg\|5vlg]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5vlm\|5vlm]], [[5vlg\|5vlg]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Entcl_2353 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1334193 "Enterobacter lignolyticus" Deangelis et al. 2011])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5vl9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5vl9 OCA], [http://pdbe.org/5vl9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5vl9 RCSB], [http://www.ebi.ac.uk/pdbsum/5vl9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5vl9 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5vl9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5vl9 OCA], [http://pdbe.org/5vl9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5vl9 RCSB], [http://www.ebi.ac.uk/pdbsum/5vl9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5vl9 ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Tightly regulated promoters are essential for numerous biological applications, where strong inducibility, portability, and scalability are desirable. Current systems are often incompatible with large-scale fermentations due to high inducer costs and strict media requirements. Here, we describe the bottom-up engineering of 'Jungle Express', an expression system that enables efficient gene regulation in diverse proteobacteria. This system is guided by EilR, a multidrug-binding repressor with high affinity to its optimized operator and cationic dyes that act as powerful inducers at negligible costs. In E. coli, the engineered promoters exhibit minimal basal transcription and are inducible over four orders of magnitude by 1 microM crystal violet, reaching expression levels exceeding those of the strongest current bacterial systems. Further, we provide molecular insights into specific interactions of EilR with its operator and with two inducers. The versatility of Jungle Express opens the way for tightly controlled and efficient gene expression that is not restricted to host organism, substrate, or scale.
		+
		+	Jungle Express is a versatile repressor system for tight transcriptional control.,Ruegg TL, Pereira JH, Chen JC, DeGiovanni A, Novichkov P, Mutalik VK, Tomaleri GP, Singer SW, Hillson NJ, Simmons BA, Adams PD, Thelen MP Nat Commun. 2018 Sep 6;9(1):3617. doi: 10.1038/s41467-018-05857-3. PMID:30190458<ref>PMID:30190458</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5vl9" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Enterobacter lignolyticus deangelis et al. 2011]]
	[[Category: Adams, P D]]		[[Category: Adams, P D]]
	[[Category: Chen, J]]		[[Category: Chen, J]]

OCA at 05:26, 27 June 2018

OCA — Wed, 27 Jun 2018 05:26:33 GMT

←Older revision		Revision as of 05:26, 27 June 2018
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-	'''Unreleased structure'''

-	~~The entry~~ 5vl9 is ~~ON HOLD until Paper Publication~~	+	==Crystal structure of EilR in complex with eilO DNA element==
-		+	<StructureSection load='5vl9' size='340' side='right' caption='[[5vl9]], [[Resolution\|resolution]] 2.16Å' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5vl9]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VL9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5VL9 FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEZ:HEXANE-1,6-DIOL'>HEZ</scene></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5vlm\|5vlm]], [[5vlg\|5vlg]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5vl9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5vl9 OCA], [http://pdbe.org/5vl9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5vl9 RCSB], [http://www.ebi.ac.uk/pdbsum/5vl9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5vl9 ProSAT]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Adams, P D]]
		+	[[Category: Chen, J]]
		+	[[Category: DeGiovani, A]]
		+	[[Category: Novichkov, P]]
		+	[[Category: Pereira, J H]]
		+	[[Category: Ruegg, T L]]
		+	[[Category: Simmons, B]]
		+	[[Category: Singer, S]]
		+	[[Category: Thelen, M]]
		+	[[Category: Tomaleri, G P]]
		+	[[Category: Protein-dna complex]]
		+	[[Category: Transcription factor]]
		+	[[Category: Transcription-dna complex]]

OCA at 14:06, 27 September 2017

OCA — Wed, 27 Sep 2017 14:06:26 GMT

←Older revision		Revision as of 14:06, 27 September 2017
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5vl9 is ON HOLD	+	The entry 5vl9 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "5vl9" [edit=sysop:move=sysop]

OCA — Thu, 04 May 2017 11:26:29 GMT

Protected "5vl9" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:26, 4 May 2017

OCA: New page: '''Unreleased structure''' The entry 5vl9 is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Thu, 04 May 2017 11:26:27 GMT

New page: '''Unreleased structure''' The entry 5vl9 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 5vl9 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]