5vlg - Revision history

OCA at 14:11, 13 March 2024

OCA — Wed, 13 Mar 2024 14:11:06 GMT

←Older revision		Revision as of 14:11, 13 March 2024
Line 3:		Line 3:
	<StructureSection load='5vlg' size='340' side='right'caption='[[5vlg]], [[Resolution\|resolution]] 2.93Å' scene=''>		<StructureSection load='5vlg' size='340' side='right'caption='[[5vlg]], [[Resolution\|resolution]] 2.93Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5vlg]] is a 8 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"enterobacter_lignolyticus"_deangelis_et_al._2011 "enterobacter~~ lignolyticus~~" deangelis et al. 2011~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VLG OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5VLG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5vlg]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterobacter_lignolyticus Enterobacter lignolyticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VLG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5VLG FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat"~~><scene name~~=~~'pdbligand=MGR:MALACHITE+GREEN'>MGR</scene~~></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.932Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat">~~[[5vlm\|5vlm]], [[5vl9\|5vl9]]~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MGR:MALACHITE+GREEN'>MGR</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5vlg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5vlg OCA], [~~http~~://pdbe.org/5vlg PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5vlg RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5vlg PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5vlg ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5vlg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5vlg OCA], [https://pdbe.org/5vlg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5vlg RCSB], [https://www.ebi.ac.uk/pdbsum/5vlg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5vlg ProSAT]</span></td></tr>
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/E3G817_ENTLS E3G817_ENTLS]
-	Tightly regulated promoters are essential for numerous biological applications, where strong inducibility, portability, and scalability are desirable. Current systems are often incompatible with large-scale fermentations due to high inducer costs and strict media requirements. Here, we describe the bottom-up engineering of 'Jungle Express', an expression system that enables efficient gene regulation in diverse proteobacteria. This system is guided by EilR, a multidrug-binding repressor with high affinity to its optimized operator and cationic dyes that act as powerful inducers at negligible costs. In E. coli, the engineered promoters exhibit minimal basal transcription and are inducible over four orders of magnitude by 1 microM crystal violet, reaching expression levels exceeding those of the strongest current bacterial systems. Further, we provide molecular insights into specific interactions of EilR with its operator and with two inducers. ~~The versatility of Jungle Express opens the way for tightly controlled and efficient gene expression that is not restricted to host organism, substrate, or scale~~.	+

-	Jungle Express is a versatile repressor system for tight transcriptional control.,Ruegg TL, Pereira JH, Chen JC, DeGiovanni A, Novichkov P, Mutalik VK, Tomaleri GP, Singer SW, Hillson NJ, Simmons BA, Adams PD, Thelen MP Nat Commun. 2018 Sep 6;9(1):3617. doi: 10.1038/s41467-018-05857-3. PMID:30190458<ref>PMID:30190458</ref>	+	==See Also==
-		+	*[[Tetracycline repressor protein 3D structures\|Tetracycline repressor protein 3D structures]]
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class~~=~~"pdbe-citations 5vlg" style~~=~~"background-color:#fffaf0;"></div>~~	+
-	~~== References~~ ==	+
-	~~<references/>~~	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Enterobacter lignolyticus deangelis et al. 2011]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Adams~~, P D~~]]	+	[[Category: Adams PD]]
-	[[Category: Chen, J]]	+	[[Category: Chen J]]
-	[[Category: DeGiovani, A]]	+	[[Category: DeGiovani A]]
-	[[Category: Novichkov, P]]	+	[[Category: Novichkov P]]
-	[[Category: Pereira~~, J H~~]]	+	[[Category: Pereira JH]]
-	[[Category: Ruegg~~, T L~~]]	+	[[Category: Ruegg TL]]
-	[[Category: Simmons, B]]	+	[[Category: Simmons B]]
-	[[Category: Singer, S]]	+	[[Category: Singer S]]
-	[[Category: Thelen, M]]	+	[[Category: Thelen M]]
-	[[Category: Tomaleri~~, G P]]~~	+	[[Category: Tomaleri GP]]
-	~~[[Category: Transcription]]~~	+
-	~~[[Category: Transcription factor~~]]	+

OCA at 09:36, 4 December 2019

OCA — Wed, 04 Dec 2019 09:36:54 GMT

←Older revision		Revision as of 09:36, 4 December 2019
Line 1:		Line 1:

	==Crystal structure of EilR in complex with malachite green==		==Crystal structure of EilR in complex with malachite green==
-	<StructureSection load='5vlg' size='340' side='right' caption='[[5vlg]], [[Resolution\|resolution]] 2.93Å' scene=''>	+	<StructureSection load='5vlg' size='340' side='right'caption='[[5vlg]], [[Resolution\|resolution]] 2.93Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5vlg]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/"enterobacter_lignolyticus"_deangelis_et_al._2011 "enterobacter lignolyticus" deangelis et al. 2011]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VLG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5VLG FirstGlance]. <br>		<table><tr><td colspan='2'>[[5vlg]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/"enterobacter_lignolyticus"_deangelis_et_al._2011 "enterobacter lignolyticus" deangelis et al. 2011]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VLG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5VLG FirstGlance]. <br>
Line 22:		Line 22:
	</StructureSection>		</StructureSection>
	[[Category: Enterobacter lignolyticus deangelis et al. 2011]]		[[Category: Enterobacter lignolyticus deangelis et al. 2011]]
		+	[[Category: Large Structures]]
	[[Category: Adams, P D]]		[[Category: Adams, P D]]
	[[Category: Chen, J]]		[[Category: Chen, J]]

OCA at 20:47, 2 December 2018

OCA — Sun, 02 Dec 2018 20:47:20 GMT

←Older revision		Revision as of 20:47, 2 December 2018
Line 3:		Line 3:
	<StructureSection load='5vlg' size='340' side='right' caption='[[5vlg]], [[Resolution\|resolution]] 2.93Å' scene=''>		<StructureSection load='5vlg' size='340' side='right' caption='[[5vlg]], [[Resolution\|resolution]] 2.93Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5vlg]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VLG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5VLG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5vlg]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/"enterobacter_lignolyticus"_deangelis_et_al._2011 "enterobacter lignolyticus" deangelis et al. 2011]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VLG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5VLG FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MGR:MALACHITE+GREEN'>MGR</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MGR:MALACHITE+GREEN'>MGR</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5vlm\|5vlm]], [[5vl9\|5vl9]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5vlm\|5vlm]], [[5vl9\|5vl9]]</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5vlg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5vlg OCA], [http://pdbe.org/5vlg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5vlg RCSB], [http://www.ebi.ac.uk/pdbsum/5vlg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5vlg ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5vlg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5vlg OCA], [http://pdbe.org/5vlg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5vlg RCSB], [http://www.ebi.ac.uk/pdbsum/5vlg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5vlg ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Tightly regulated promoters are essential for numerous biological applications, where strong inducibility, portability, and scalability are desirable. Current systems are often incompatible with large-scale fermentations due to high inducer costs and strict media requirements. Here, we describe the bottom-up engineering of 'Jungle Express', an expression system that enables efficient gene regulation in diverse proteobacteria. This system is guided by EilR, a multidrug-binding repressor with high affinity to its optimized operator and cationic dyes that act as powerful inducers at negligible costs. In E. coli, the engineered promoters exhibit minimal basal transcription and are inducible over four orders of magnitude by 1 microM crystal violet, reaching expression levels exceeding those of the strongest current bacterial systems. Further, we provide molecular insights into specific interactions of EilR with its operator and with two inducers. The versatility of Jungle Express opens the way for tightly controlled and efficient gene expression that is not restricted to host organism, substrate, or scale.
		+
		+	Jungle Express is a versatile repressor system for tight transcriptional control.,Ruegg TL, Pereira JH, Chen JC, DeGiovanni A, Novichkov P, Mutalik VK, Tomaleri GP, Singer SW, Hillson NJ, Simmons BA, Adams PD, Thelen MP Nat Commun. 2018 Sep 6;9(1):3617. doi: 10.1038/s41467-018-05857-3. PMID:30190458<ref>PMID:30190458</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5vlg" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Enterobacter lignolyticus deangelis et al. 2011]]
	[[Category: Adams, P D]]		[[Category: Adams, P D]]
	[[Category: Chen, J]]		[[Category: Chen, J]]

OCA at 05:26, 27 June 2018

OCA — Wed, 27 Jun 2018 05:26:33 GMT

←Older revision		Revision as of 05:26, 27 June 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5vlg is ~~ON HOLD until Paper Publication~~	+	==Crystal structure of EilR in complex with malachite green==
-		+	<StructureSection load='5vlg' size='340' side='right' caption='[[5vlg]], [[Resolution\|resolution]] 2.93Å' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5vlg]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VLG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5VLG FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MGR:MALACHITE+GREEN'>MGR</scene></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5vlm\|5vlm]], [[5vl9\|5vl9]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5vlg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5vlg OCA], [http://pdbe.org/5vlg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5vlg RCSB], [http://www.ebi.ac.uk/pdbsum/5vlg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5vlg ProSAT]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Adams, P D]]
		+	[[Category: Chen, J]]
		+	[[Category: DeGiovani, A]]
		+	[[Category: Novichkov, P]]
		+	[[Category: Pereira, J H]]
		+	[[Category: Ruegg, T L]]
		+	[[Category: Simmons, B]]
		+	[[Category: Singer, S]]
		+	[[Category: Thelen, M]]
		+	[[Category: Tomaleri, G P]]
		+	[[Category: Transcription]]
		+	[[Category: Transcription factor]]

OCA at 14:06, 27 September 2017

OCA — Wed, 27 Sep 2017 14:06:34 GMT

←Older revision		Revision as of 14:06, 27 September 2017
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5vlg is ON HOLD	+	The entry 5vlg is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "5vlg" [edit=sysop:move=sysop]

OCA — Thu, 04 May 2017 11:26:48 GMT

Protected "5vlg" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:26, 4 May 2017

OCA: New page: '''Unreleased structure''' The entry 5vlg is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Thu, 04 May 2017 11:26:47 GMT

New page: '''Unreleased structure''' The entry 5vlg is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 5vlg is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]