5vnw - Revision history

OCA at 13:52, 4 October 2023

OCA — Wed, 04 Oct 2023 13:52:41 GMT

←Older revision		Revision as of 13:52, 4 October 2023
Line 1:		Line 1:

	==Crystal structure of Nb.b201 bound to human serum albumin==		==Crystal structure of Nb.b201 bound to human serum albumin==
-	<StructureSection load='5vnw' size='340' side='right' caption='[[5vnw]], [[Resolution\|resolution]] 2.60Å' scene=''>	+	<StructureSection load='5vnw' size='340' side='right'caption='[[5vnw]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5vnw]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~] and [~~http~~://en.wikipedia.org/wiki/~~Synthetic_construct_sequences~~ Synthetic construct ~~sequences~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VNW OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5VNW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5vnw]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VNW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5VNW FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BUA:BUTANOIC+ACID'>BUA</scene>, <scene name='pdbligand=DAO:LAURIC+ACID'>DAO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PLM:PALMITIC+ACID'>PLM</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.6Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5vnv\|5vnv]]</td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BUA:BUTANOIC+ACID'>BUA</scene>, <scene name='pdbligand=DAO:LAURIC+ACID'>DAO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PLM:PALMITIC+ACID'>PLM</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ALB, GIG20, GIG42, PRO0903, PRO1708, PRO2044, PRO2619, PRO2675, UNQ696/PRO1341 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5vnw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5vnw OCA], [https://pdbe.org/5vnw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5vnw RCSB], [https://www.ebi.ac.uk/pdbsum/5vnw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5vnw ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5vnw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5vnw OCA], [~~http~~://pdbe.org/5vnw PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5vnw RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5vnw PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5vnw ProSAT]</span></td></tr>	+
	</table>		</table>
	== Disease ==		== Disease ==
-	[~~[http~~://www.uniprot.org/uniprot/ALBU_HUMAN ALBU_HUMAN]] Defects in ALB are a cause of familial dysalbuminemic hyperthyroxinemia (FDH) [MIM:[~~http~~://omim.org/entry/103600 103600]]. FDH is a form of euthyroid hyperthyroxinemia that is due to increased affinity of ALB for T(4). It is the most common cause of inherited euthyroid hyperthyroxinemia in Caucasian population.<ref>PMID:8048949</ref> <ref>PMID:7852505</ref> <ref>PMID:9329347</ref> <ref>PMID:9589637</ref>	+	[https://www.uniprot.org/uniprot/ALBU_HUMAN ALBU_HUMAN] Defects in ALB are a cause of familial dysalbuminemic hyperthyroxinemia (FDH) [MIM:[https://omim.org/entry/103600 103600]. FDH is a form of euthyroid hyperthyroxinemia that is due to increased affinity of ALB for T(4). It is the most common cause of inherited euthyroid hyperthyroxinemia in Caucasian population.<ref>PMID:8048949</ref> <ref>PMID:7852505</ref> <ref>PMID:9329347</ref> <ref>PMID:9589637</ref>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/ALBU_HUMAN ALBU_HUMAN]] Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc.<ref>PMID:19021548</ref>	+	[https://www.uniprot.org/uniprot/ALBU_HUMAN ALBU_HUMAN] Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc.<ref>PMID:19021548</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 24:		Line 23:

	==See Also==		==See Also==
-	*[[Albumin\|Albumin]]	+	*[[Albumin 3D structures\|Albumin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
-	[[Category: ~~Synthetic construct sequences~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Kruse, A C~~]]	+	[[Category: Synthetic construct]]
-	[[Category: ~~McMahon, C~~]]	+	[[Category: Kruse AC]]
-	[[Category: ~~Camelid]]~~	+	[[Category: McMahon C]]
-	~~[[Category: Llama]]~~	+
-	~~[[Category: Nanobody]]~~	+
-	~~[[Category: Synthetic protein]]~~	+
-	~~[[Category: Transport protein-de novo protein complex~~]]	+

OCA at 06:53, 14 March 2018

OCA — Wed, 14 Mar 2018 06:53:20 GMT

←Older revision		Revision as of 06:53, 14 March 2018
Line 3:		Line 3:
	<StructureSection load='5vnw' size='340' side='right' caption='[[5vnw]], [[Resolution\|resolution]] 2.60Å' scene=''>		<StructureSection load='5vnw' size='340' side='right' caption='[[5vnw]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5vnw]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VNW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5VNW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5vnw]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human] and [http://en.wikipedia.org/wiki/Synthetic_construct_sequences Synthetic construct sequences]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VNW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5VNW FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BUA:BUTANOIC+ACID'>BUA</scene>, <scene name='pdbligand=DAO:LAURIC+ACID'>DAO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PLM:PALMITIC+ACID'>PLM</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BUA:BUTANOIC+ACID'>BUA</scene>, <scene name='pdbligand=DAO:LAURIC+ACID'>DAO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PLM:PALMITIC+ACID'>PLM</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5vnv\|5vnv]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5vnv\|5vnv]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ALB, GIG20, GIG42, PRO0903, PRO1708, PRO2044, PRO2619, PRO2675, UNQ696/PRO1341 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5vnw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5vnw OCA], [http://pdbe.org/5vnw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5vnw RCSB], [http://www.ebi.ac.uk/pdbsum/5vnw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5vnw ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5vnw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5vnw OCA], [http://pdbe.org/5vnw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5vnw RCSB], [http://www.ebi.ac.uk/pdbsum/5vnw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5vnw ProSAT]</span></td></tr>
	</table>		</table>
Line 21:		Line 22:
	</div>		</div>
	<div class="pdbe-citations 5vnw" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5vnw" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Albumin\|Albumin]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
		+	[[Category: Synthetic construct sequences]]
	[[Category: Kruse, A C]]		[[Category: Kruse, A C]]
	[[Category: McMahon, C]]		[[Category: McMahon, C]]

OCA at 07:16, 22 February 2018

OCA — Thu, 22 Feb 2018 07:16:32 GMT

←Older revision		Revision as of 07:16, 22 February 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5vnw is ~~ON HOLD~~ ~~until Paper~~ Publication	+	==Crystal structure of Nb.b201 bound to human serum albumin==
		+	<StructureSection load='5vnw' size='340' side='right' caption='[[5vnw]], [[Resolution\|resolution]] 2.60Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5vnw]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VNW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5VNW FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BUA:BUTANOIC+ACID'>BUA</scene>, <scene name='pdbligand=DAO:LAURIC+ACID'>DAO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PLM:PALMITIC+ACID'>PLM</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5vnv\|5vnv]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5vnw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5vnw OCA], [http://pdbe.org/5vnw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5vnw RCSB], [http://www.ebi.ac.uk/pdbsum/5vnw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5vnw ProSAT]</span></td></tr>
		+	</table>
		+	== Disease ==
		+	[[http://www.uniprot.org/uniprot/ALBU_HUMAN ALBU_HUMAN]] Defects in ALB are a cause of familial dysalbuminemic hyperthyroxinemia (FDH) [MIM:[http://omim.org/entry/103600 103600]]. FDH is a form of euthyroid hyperthyroxinemia that is due to increased affinity of ALB for T(4). It is the most common cause of inherited euthyroid hyperthyroxinemia in Caucasian population.<ref>PMID:8048949</ref> <ref>PMID:7852505</ref> <ref>PMID:9329347</ref> <ref>PMID:9589637</ref>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/ALBU_HUMAN ALBU_HUMAN]] Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc.<ref>PMID:19021548</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Camelid single-domain antibody fragments ('nanobodies') provide the remarkable specificity of antibodies within a single 15-kDa immunoglobulin VHH domain. This unique feature has enabled applications ranging from use as biochemical tools to therapeutic agents. Nanobodies have emerged as especially useful tools in protein structural biology, facilitating studies of conformationally dynamic proteins such as G-protein-coupled receptors (GPCRs). Nearly all nanobodies available to date have been obtained by animal immunization, a bottleneck restricting many applications of this technology. To solve this problem, we report a fully in vitro platform for nanobody discovery based on yeast surface display. We provide a blueprint for identifying nanobodies, demonstrate the utility of the library by crystallizing a nanobody with its antigen, and most importantly, we utilize the platform to discover conformationally selective nanobodies to two distinct human GPCRs. To facilitate broad deployment of this platform, the library and associated protocols are freely available for nonprofit research.

-	~~Authors: McMahon~~, C., ~~Kruse~~, A.C.	+	Yeast surface display platform for rapid discovery of conformationally selective nanobodies.,McMahon C, Baier AS, Pascolutti R, Wegrecki M, Zheng S, Ong JX, Erlandson SC, Hilger D, Rasmussen SGF, Ring AM, Manglik A, Kruse AC Nat Struct Mol Biol. 2018 Feb 12. pii: 10.1038/s41594-018-0028-6. doi:, 10.1038/s41594-018-0028-6. PMID:29434346<ref>PMID:29434346</ref>

-	~~Description: Crystal structure~~ of Nb.~~b201 bound to human serum albumin~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
-	[[Category: ~~Kruse~~, A.C]]	+	<div class="pdbe-citations 5vnw" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Mcmahon, C~~]]	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Kruse, A C]]
		+	[[Category: McMahon, C]]
		+	[[Category: Camelid]]
		+	[[Category: Llama]]
		+	[[Category: Nanobody]]
		+	[[Category: Synthetic protein]]
		+	[[Category: Transport protein-de novo protein complex]]

OCA at 14:08, 27 September 2017

OCA — Wed, 27 Sep 2017 14:08:08 GMT

←Older revision		Revision as of 14:08, 27 September 2017
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5vnw is ON HOLD	+	The entry 5vnw is ON HOLD until Paper Publication

	Authors: McMahon, C., Kruse, A.C.		Authors: McMahon, C., Kruse, A.C.

OCA at 14:02, 17 May 2017

OCA — Wed, 17 May 2017 14:02:11 GMT

←Older revision		Revision as of 14:02, 17 May 2017
Line 3:		Line 3:
	The entry 5vnw is ON HOLD		The entry 5vnw is ON HOLD

-	Authors: ~~Conor~~ McMahon, ~~Andrew~~ C. Kruse	+	Authors: McMahon, C., Kruse, A.C.

	Description: Crystal structure of Nb.b201 bound to human serum albumin		Description: Crystal structure of Nb.b201 bound to human serum albumin
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: ~~Conor~~ Mcmahon, ~~Andrew~~ C~~. Kruse~~]]	+	[[Category: Kruse, A.C]]
		+	[[Category: Mcmahon, C]]

OCA: Protected "5vnw" [edit=sysop:move=sysop]

OCA — Wed, 10 May 2017 11:14:20 GMT

Protected "5vnw" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:14, 10 May 2017

OCA: New page: '''Unreleased structure''' The entry 5vnw is ON HOLD Authors: Conor McMahon, Andrew C. Kruse Description: Crystal structure of Nb.b201 bound to human serum albumin [[Category: Unreleas...

OCA — Wed, 10 May 2017 11:14:19 GMT

New page: '''Unreleased structure''' The entry 5vnw is ON HOLD Authors: Conor McMahon, Andrew C. Kruse Description: Crystal structure of Nb.b201 bound to human serum albumin [[Category: Unreleas...

New page

'''Unreleased structure'''

The entry 5vnw is ON HOLD

Authors: Conor McMahon, Andrew C. Kruse

Description: Crystal structure of Nb.b201 bound to human serum albumin
[[Category: Unreleased Structures]]
[[Category: Conor Mcmahon, Andrew C. Kruse]]