OCA at 10:05, 16 November 2017

OCA — Thu, 16 Nov 2017 10:05:50 GMT

←Older revision		Revision as of 10:05, 16 November 2017
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-	'''Unreleased structure'''

-	~~The entry~~ 5vny is ~~ON HOLD until Paper~~ Publication	+	==Crystal structure of DM14-3 domain of Lgd==
		+	<StructureSection load='5vny' size='340' side='right' caption='[[5vny]], [[Resolution\|resolution]] 1.10Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5vny]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Drome Drome]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VNY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5VNY FirstGlance]. <br>
		+	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">l(2)gd1, CG4713, Dmel_CG4713 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 DROME])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5vny FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5vny OCA], [http://pdbe.org/5vny PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5vny RCSB], [http://www.ebi.ac.uk/pdbsum/5vny PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5vny ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The ESCRT-III complex induces outward membrane budding and fission through homotypic polymerization of its core component Shrub/CHMP4B. Shrub activity is regulated by its direct interaction with a protein called Lgd in flies, or CC2D1A or B in humans. Here, we report the structural basis for this interaction and propose a mechanism for regulation of polymer assembly. The isolated third DM14 repeat of Lgd binds Shrub, and an Lgd fragment containing only this DM14 repeat and its C-terminal C2 domain is sufficient for in vivo function. The DM14 domain forms a helical hairpin with a conserved, positively charged tip, that, in the structure of a DM14 domain-Shrub complex, occupies a negatively charged surface of Shrub that is otherwise used for homopolymerization. Lgd mutations at this interface disrupt its function in flies, confirming functional importance. Together, these data argue that Lgd regulates ESCRT activity by controlling access to the Shrub self-assembly surface.

-	~~Authors:~~ McMillan, ~~B.J.~~, Blacklow, S.C.	+	Structural Basis for Regulation of ESCRT-III Complexes by Lgd.,McMillan BJ, Tibbe C, Drabek AA, Seegar TCM, Blacklow SC, Klein T Cell Rep. 2017 May 30;19(9):1750-1757. doi: 10.1016/j.celrep.2017.05.026. PMID:28564595<ref>PMID:28564595</ref>

-	~~Description: Crystal structure~~ of ~~DM14-3 domain~~ of ~~Lgd~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
-	[[Category: ~~Mcmillan~~, B.J]]	+	<div class="pdbe-citations 5vny" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Blacklow, S.C~~]]	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Drome]]
		+	[[Category: Blacklow, S C]]
		+	[[Category: McMillan, B J]]
		+	[[Category: Endocytosis]]
		+	[[Category: Escrt]]
		+	[[Category: Protein binding]]

OCA at 11:40, 24 May 2017

OCA — Wed, 24 May 2017 11:40:18 GMT

←Older revision		Revision as of 11:40, 24 May 2017
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5vny is ON HOLD	+	The entry 5vny is ON HOLD until Paper Publication

	Authors: McMillan, B.J., Blacklow, S.C.		Authors: McMillan, B.J., Blacklow, S.C.

OCA: Protected "5vny" [edit=sysop:move=sysop]

OCA — Wed, 10 May 2017 11:14:29 GMT

Protected "5vny" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:14, 10 May 2017

OCA: New page: '''Unreleased structure''' The entry 5vny is ON HOLD Authors: McMillan, B.J., Blacklow, S.C. Description: Crystal structure of DM14-3 domain of Lgd Category: Unreleased Structures ...

OCA — Wed, 10 May 2017 11:14:27 GMT

New page: '''Unreleased structure''' The entry 5vny is ON HOLD Authors: McMillan, B.J., Blacklow, S.C. Description: Crystal structure of DM14-3 domain of Lgd Category: Unreleased Structures ...

New page

'''Unreleased structure'''

The entry 5vny is ON HOLD

Authors: McMillan, B.J., Blacklow, S.C.

Description: Crystal structure of DM14-3 domain of Lgd
[[Category: Unreleased Structures]]
[[Category: Mcmillan, B.J]]
[[Category: Blacklow, S.C]]

5vny - Revision history

OCA at 10:05, 16 November 2017

OCA at 11:40, 24 May 2017

OCA: Protected "5vny" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 5vny is ON HOLD Authors: McMillan, B.J., Blacklow, S.C. Description: Crystal structure of DM14-3 domain of Lgd Category: Unreleased Structures ...