5voh - Revision history

OCA at 13:53, 4 October 2023

OCA — Wed, 04 Oct 2023 13:53:05 GMT

←Older revision		Revision as of 13:53, 4 October 2023
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	==Crystal structure of engineered water-forming NADPH oxidase (TPNOX) bound to NADPH. The G159A, D177A, A178R, M179S, P184R mutant of LbNOX.==		==Crystal structure of engineered water-forming NADPH oxidase (TPNOX) bound to NADPH. The G159A, D177A, A178R, M179S, P184R mutant of LbNOX.==
-	<StructureSection load='5voh' size='340' side='right' caption='[[5voh]], [[Resolution\|resolution]] 2.30Å' scene=''>	+	<StructureSection load='5voh' size='340' side='right'caption='[[5voh]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5voh]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Lactobacillus_brevis_kb290 Lactobacillus~~ brevis ~~kb290~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VOH OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5VOH FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5voh]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Levilactobacillus_brevis_KB290 Levilactobacillus brevis KB290]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VOH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5VOH FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.302Å</td></tr>
-	~~<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSO:S-HYDROXYCYSTEINE'>CSO</scene></td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CSO:S-HYDROXYCYSTEINE'>CSO</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">LVISKB_1491 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1001583 Lactobacillus brevis KB290])</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5voh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5voh OCA], [https://pdbe.org/5voh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5voh RCSB], [https://www.ebi.ac.uk/pdbsum/5voh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5voh ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5voh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5voh OCA], [~~http~~://pdbe.org/5voh PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5voh RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5voh PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5voh ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/M5B0V2_LEVBR M5B0V2_LEVBR]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Lactobacillus brevis kb290~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Cracan, V~~]]	+	[[Category: Levilactobacillus brevis KB290]]
-	[[Category: ~~Grabarek, Z~~]]	+	[[Category: Cracan V]]
-	[[Category: ~~Nadph]]~~	+	[[Category: Grabarek Z]]
-	~~[[Category: Oxidoreductase]]~~	+
-	~~[[Category: Redox active cysteine]]~~	+
-	~~[[Category: Water-forming oxidase~~]]	+

OCA at 14:41, 6 November 2017

OCA — Mon, 06 Nov 2017 14:41:51 GMT

←Older revision		Revision as of 14:41, 6 November 2017
Line 3:		Line 3:
	<StructureSection load='5voh' size='340' side='right' caption='[[5voh]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='5voh' size='340' side='right' caption='[[5voh]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5voh]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VOH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5VOH FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5voh]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Lactobacillus_brevis_kb290 Lactobacillus brevis kb290]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VOH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5VOH FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSO:S-HYDROXYCYSTEINE'>CSO</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSO:S-HYDROXYCYSTEINE'>CSO</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">LVISKB_1491 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1001583 Lactobacillus brevis KB290])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5voh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5voh OCA], [http://pdbe.org/5voh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5voh RCSB], [http://www.ebi.ac.uk/pdbsum/5voh PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5voh ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5voh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5voh OCA], [http://pdbe.org/5voh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5voh RCSB], [http://www.ebi.ac.uk/pdbsum/5voh PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5voh ProSAT]</span></td></tr>
	</table>		</table>
Line 21:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Lactobacillus brevis kb290]]
	[[Category: Cracan, V]]		[[Category: Cracan, V]]
	[[Category: Grabarek, Z]]		[[Category: Grabarek, Z]]

OCA at 04:03, 30 August 2017

OCA — Wed, 30 Aug 2017 04:03:53 GMT

←Older revision		Revision as of 04:03, 30 August 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	The ~~entry~~ 5voh is ~~ON HOLD~~	+	==Crystal structure of engineered water-forming NADPH oxidase (TPNOX) bound to NADPH. The G159A, D177A, A178R, M179S, P184R mutant of LbNOX.==
		+	<StructureSection load='5voh' size='340' side='right' caption='[[5voh]], [[Resolution\|resolution]] 2.30Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5voh]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VOH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5VOH FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSO:S-HYDROXYCYSTEINE'>CSO</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5voh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5voh OCA], [http://pdbe.org/5voh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5voh RCSB], [http://www.ebi.ac.uk/pdbsum/5voh PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5voh ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The redox coenzymes NADH and NADPH are broadly required for energy metabolism, biosynthesis and detoxification. Despite detailed knowledge of specific enzymes and pathways that utilize these coenzymes, a holistic understanding of the regulation and compartmentalization of NADH- and NADPH-dependent pathways is lacking, partly because of a lack of tools with which to investigate these processes in living cells. We have previously reported the use of the naturally occurring Lactobacillus brevis H2O-forming NADH oxidase (LbNOX) as a genetic tool for manipulation of the NAD+/NADH ratio in human cells. Here, we present triphosphopyridine nucleotide oxidase (TPNOX), a rationally designed and engineered mutant of LbNOX that is strictly specific to NADPH. We characterized the effects of TPNOX expression on cellular metabolism and used it in combination with LbNOX to show how the redox states of mitochondrial NADPH and NADH pools are connected.

-	~~Authors~~:	+	A genetically encoded tool for manipulation of NADP+/NADPH in living cells.,Cracan V, Titov DV, Shen H, Grabarek Z, Mootha VK Nat Chem Biol. 2017 Aug 7. doi: 10.1038/nchembio.2454. PMID:28805804<ref>PMID:28805804</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 5voh" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Cracan, V]]
		+	[[Category: Grabarek, Z]]
		+	[[Category: Nadph]]
		+	[[Category: Oxidoreductase]]
		+	[[Category: Redox active cysteine]]
		+	[[Category: Water-forming oxidase]]

OCA: Protected "5voh" [edit=sysop:move=sysop]

OCA — Wed, 10 May 2017 11:15:51 GMT

Protected "5voh" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:15, 10 May 2017

OCA: New page: '''Unreleased structure''' The entry 5voh is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 10 May 2017 11:15:50 GMT

New page: '''Unreleased structure''' The entry 5voh is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 5voh is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]